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The map label for this gene is nfi [H]

Identifier: 124028411

GI number: 124028411

Start: 1574593

End: 1575336

Strand: Direct

Name: nfi [H]

Synonym: Hbut_1565

Alternate gene names: 124028411

Gene position: 1574593-1575336 (Clockwise)

Preceding gene: 124028410

Following gene: 124028413

Centisome position: 94.45

GC content: 50.81

Gene sequence:

>744_bases
TTGACATACAGCTCGGTTGCAAAGCTCATAGAGCTACAGAAGAGGCTCTCCAGGAGGATAGTCTCAAGCCTAAAACCCAT
GGATACAGCAAGCATAAGAAGAATAGTCGGCCTAGATGCCGCGTACTCCAAGCGGTACGGTGGAGTTGCAATAGCAGCCC
TTACAACCCGCGACGGCCAGCTACTCACATACTCGGTTGCACTAGGAGAGCCACCACTGCAATACATACCCGGCCTACTA
GCCTTTCGAGAAGCCCCACTCTTCTACACAGCGCTACGCCTACTGGACAGTAATTATGATCTAGTAGTTGTTGACGGCCA
CGGCATCTCGCACCCCCGCAGAGCTGGCATAGCATCACACATAGGTATAGCTATAGCAAAGCCCTCCATAGGTGTTGCAA
AAAAGAAGCTTTACGGCCACGAGCAGATAGTAAGCGAGGACTGTAAACCACCCTGCATAGCCGGGTACGTGGTAGATGAA
GATACCGGAGAGAAGCTTGCAGCAATAGTTAGGACTGGAAGGAGCAAAAAGAGCCGCCTATACGTAAGCCCAGGAGCCTA
CACAGACCTAGACTCTGCTACTACTATAGTACTTGAACTCCTAGAGGCTAGGAAGACACCACTACCCGCACCCACATATC
ATGCTGACCGCATATCAAAGACTATAGCACGACAGCTGGACAGCGGCGAGCTAAGCCCGAGAAGCCTTAAGAAAAGACAC
CGCACATTACTAGACTACATCTAA

Upstream 100 bases:

>100_bases
GGCGACGGGATAGACCTGCTAGTGATACGCAGGGAGGAGGACAGCATAAAGGCCGAAGAGGAGTCCATAACAATCTAGAC
AGCCTCTGGGGCGGCTGTTT

Downstream 100 bases:

>100_bases
GCATAAGCTATGTGCAAAAGCAATCAGTGTACTCCTCAGCCTATCACACCTGTGACTCGTGCAAGCCTCTCAGCTGCTTC
CCACGTGAGGCCAGATTCTC

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MTYSSVAKLIELQKRLSRRIVSSLKPMDTASIRRIVGLDAAYSKRYGGVAIAALTTRDGQLLTYSVALGEPPLQYIPGLL
AFREAPLFYTALRLLDSNYDLVVVDGHGISHPRRAGIASHIGIAIAKPSIGVAKKKLYGHEQIVSEDCKPPCIAGYVVDE
DTGEKLAAIVRTGRSKKSRLYVSPGAYTDLDSATTIVLELLEARKTPLPAPTYHADRISKTIARQLDSGELSPRSLKKRH
RTLLDYI

Sequences:

>Translated_247_residues
MTYSSVAKLIELQKRLSRRIVSSLKPMDTASIRRIVGLDAAYSKRYGGVAIAALTTRDGQLLTYSVALGEPPLQYIPGLL
AFREAPLFYTALRLLDSNYDLVVVDGHGISHPRRAGIASHIGIAIAKPSIGVAKKKLYGHEQIVSEDCKPPCIAGYVVDE
DTGEKLAAIVRTGRSKKSRLYVSPGAYTDLDSATTIVLELLEARKTPLPAPTYHADRISKTIARQLDSGELSPRSLKKRH
RTLLDYI
>Mature_246_residues
TYSSVAKLIELQKRLSRRIVSSLKPMDTASIRRIVGLDAAYSKRYGGVAIAALTTRDGQLLTYSVALGEPPLQYIPGLLA
FREAPLFYTALRLLDSNYDLVVVDGHGISHPRRAGIASHIGIAIAKPSIGVAKKKLYGHEQIVSEDCKPPCIAGYVVDED
TGEKLAAIVRTGRSKKSRLYVSPGAYTDLDSATTIVLELLEARKTPLPAPTYHADRISKTIARQLDSGELSPRSLKKRHR
TLLDYI

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Escherichia coli, GI87082357, Length=189, Percent_Identity=37.5661375661376, Blast_Score=111, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 27041; Mature: 26909

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYSSVAKLIELQKRLSRRIVSSLKPMDTASIRRIVGLDAAYSKRYGGVAIAALTTRDGQ
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHCCCCEEEEEEEECCCC
LLTYSVALGEPPLQYIPGLLAFREAPLFYTALRLLDSNYDLVVVDGHGISHPRRAGIASH
EEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHCHHHH
IGIAIAKPSIGVAKKKLYGHEQIVSEDCKPPCIAGYVVDEDTGEKLAAIVRTGRSKKSRL
HCEEEECCCCCHHHHHHHCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCEE
YVSPGAYTDLDSATTIVLELLEARKTPLPAPTYHADRISKTIARQLDSGELSPRSLKKRH
EECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
RTLLDYI
HHHHHCC
>Mature Secondary Structure 
TYSSVAKLIELQKRLSRRIVSSLKPMDTASIRRIVGLDAAYSKRYGGVAIAALTTRDGQ
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHCCCCEEEEEEEECCCC
LLTYSVALGEPPLQYIPGLLAFREAPLFYTALRLLDSNYDLVVVDGHGISHPRRAGIASH
EEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHCHHHH
IGIAIAKPSIGVAKKKLYGHEQIVSEDCKPPCIAGYVVDEDTGEKLAAIVRTGRSKKSRL
HCEEEECCCCCHHHHHHHCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCEE
YVSPGAYTDLDSATTIVLELLEARKTPLPAPTYHADRISKTIARQLDSGELSPRSLKKRH
EECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHH
RTLLDYI
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA