Click here to switch to the map view.

The map label for this gene is pckG [H]

Identifier: 124028294

GI number: 124028294

Start: 1451093

End: 1452991

Strand: Reverse

Name: pckG [H]

Synonym: Hbut_1445

Alternate gene names: 124028294

Gene position: 1452991-1451093 (Counterclockwise)

Preceding gene: 124028299

Following gene: 124028292

Centisome position: 87.15

GC content: 56.13

Gene sequence:

>1899_bases
GTGCCTAGGACCCGACTTCTTGACGAAGAGTACGACTATATGGCTGTCCTTGAGGCGCGGATGAGCCGGGATATGCTGGA
GAAGCTTCTGCGTATCCGTGACCCGGAGCTGCACCGCTGGATAGCGGATGTCATAAGGGTTACCGAACCAGCCATGGTTT
ACCTTATCACGAGTGAGGAAGACTTTGAGTATGTGAGGAAGGCTGCGCTGCGTAACCGTGAGGAACTCCCGACCCGCTAT
CCCAGGCATACGGTGCACTTTGACGGGCCTCGGGATATTGCGAGGGATCGTAAGAATACCCGCATCCTGCTGCCAGGCGG
GGAGAAGATACCCTTCATAAACACCTACGACCGGGACAAGGGACTGGCTGAGATCAGGGAGCTGCTACGCGGCATTATGC
GCGGTCGCGAGATGTTCATAGGCTTCTACTGTTTCGGGCCTAAGAGGTCGCCGTTTACCCTCCACGCTGTACAGATCACC
GACTCGGCCTACGTGGCTCACAACGAGAACATACTCTACCGTCTCTGCTACGACGAGTTCGTGGAGAAGGCTCCCAACCT
GTTCTATGCCCGGTTCATACACTCTGCGGGGGAGCGGGGCGAGAATGGCTGGAGCAAGAACATCGATAAAAGGAGGATAT
ACGTAGACCTCCACGACTACACTGTCTACAGTGTTAACACCCAGTACGGGGGTAACACTATAGGCCTCAAGAAGCTAATG
TTCCGCCTCTGTATATACAAGGGCTACCTGGAGGGCTGGCTCTGCGAACACATGTTCATAGTCGGTGTCCGTGGCCCTGG
CGGGAGGCTTACATACTTCACAGGCGCATTCCCAGCAGGCTGTGGCAAGACCTCAACGGCTTTCATCTCCGACACCATTG
TAGGCGACGACCTAGCAATCATAAAGCCCGTCAACGGCGTACCCCACGGCGTAAATCCAGAGATGGGTATGTTCGGCATA
ATTGACGGTGTCAACCCCCGGGACGACCCGGTACTCTACAAGCTCCTAACAGACCCAAGCAACGAGATAATATTCTCAAA
TGTCTTGCTCACCGATGATGGCGAGGTCTGGTGGAACGGCAAGGAGGGCGAGCCCCGGCCCGGCCTAAACTATGCTGGCC
GCTGGTGGCCCGGCAAGAAGGACGAAAACGGCAGGGAGGTACCGCCGTCACACCCCAATGCAAGGTTCACCATATCGCTC
CGCAGCCTACCCAACCTAGACCCTCGGGTCGACGACCCGATGGGCGTACCCATTAGCGCGATGATCTTTGGCGGCAGGGA
TCCTGACACCTGGGTCCCGGTGGAGGAGGCTTTTGACTGGGTACACGGCATTATAACGAAGGCCGCGGCGTTGGAGTCTG
AGAAGACTGCTGCGATACTCGAGAAGGCGGGTGTTCGGGAGCTAAACCCATTCGCCATCCTGGACTTCCTACCAATCTCC
ATAGGCAAGTTTATCCAGCTGCACCTAGACTTTGCAGAAAAGGTCGAGAAGCTGCCCAGGATATTCAGCGTCAACTACTT
CCTCCGGGACGAGCAGGGCCGCTACATAGCTGAGAAGGTTGATAAGAGGGTTTGGCTGAAGTGGATGGAGCTACGCGTCC
ACGGCGACGTAGATGCCGTACGAACTCCCACCGGGCTGATACCGGTCTACCAGGATCTGGTGAAGCTATTCGAGGAGCAC
CTTGGGAAAGATTATCCAGAGTCCCGGTACGAGAAGGAGTTCATGCTGAGAGTGCCCCAGCACCTAGCAAAGATAGAGAG
GGTGTGGAAGGTGTACGAGCAGATACCCGATACGCCCAAGGTGCTCTTCGACGTGTTGAGGAAGCAGAAGGAGAGGCTTA
AGGAGGCTCAGAGCCGCTGGGGCGACTACATATCACCCTTCAAGTTTGACCGTCGCTAG

Upstream 100 bases:

>100_bases
GACCTGCTAGTACGTCTTGAGTTTTGCCACCGTTTTTGCTGTGGTGTACGGATTTTTATTAATGCCCCACAATTGTACCT
TCTTTGCGCGGGTTTGAGCC

Downstream 100 bases:

>100_bases
CCCACCGGCAGGGCCGGCACCTGCACCCCCACAGCGCCATCGCCGCAGTCCACTACCATCTCCACGCTGAGCCTCCCCCT
ACCCATTCTCTCTGCATCTA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MPRTRLLDEEYDYMAVLEARMSRDMLEKLLRIRDPELHRWIADVIRVTEPAMVYLITSEEDFEYVRKAALRNREELPTRY
PRHTVHFDGPRDIARDRKNTRILLPGGEKIPFINTYDRDKGLAEIRELLRGIMRGREMFIGFYCFGPKRSPFTLHAVQIT
DSAYVAHNENILYRLCYDEFVEKAPNLFYARFIHSAGERGENGWSKNIDKRRIYVDLHDYTVYSVNTQYGGNTIGLKKLM
FRLCIYKGYLEGWLCEHMFIVGVRGPGGRLTYFTGAFPAGCGKTSTAFISDTIVGDDLAIIKPVNGVPHGVNPEMGMFGI
IDGVNPRDDPVLYKLLTDPSNEIIFSNVLLTDDGEVWWNGKEGEPRPGLNYAGRWWPGKKDENGREVPPSHPNARFTISL
RSLPNLDPRVDDPMGVPISAMIFGGRDPDTWVPVEEAFDWVHGIITKAAALESEKTAAILEKAGVRELNPFAILDFLPIS
IGKFIQLHLDFAEKVEKLPRIFSVNYFLRDEQGRYIAEKVDKRVWLKWMELRVHGDVDAVRTPTGLIPVYQDLVKLFEEH
LGKDYPESRYEKEFMLRVPQHLAKIERVWKVYEQIPDTPKVLFDVLRKQKERLKEAQSRWGDYISPFKFDRR

Sequences:

>Translated_632_residues
MPRTRLLDEEYDYMAVLEARMSRDMLEKLLRIRDPELHRWIADVIRVTEPAMVYLITSEEDFEYVRKAALRNREELPTRY
PRHTVHFDGPRDIARDRKNTRILLPGGEKIPFINTYDRDKGLAEIRELLRGIMRGREMFIGFYCFGPKRSPFTLHAVQIT
DSAYVAHNENILYRLCYDEFVEKAPNLFYARFIHSAGERGENGWSKNIDKRRIYVDLHDYTVYSVNTQYGGNTIGLKKLM
FRLCIYKGYLEGWLCEHMFIVGVRGPGGRLTYFTGAFPAGCGKTSTAFISDTIVGDDLAIIKPVNGVPHGVNPEMGMFGI
IDGVNPRDDPVLYKLLTDPSNEIIFSNVLLTDDGEVWWNGKEGEPRPGLNYAGRWWPGKKDENGREVPPSHPNARFTISL
RSLPNLDPRVDDPMGVPISAMIFGGRDPDTWVPVEEAFDWVHGIITKAAALESEKTAAILEKAGVRELNPFAILDFLPIS
IGKFIQLHLDFAEKVEKLPRIFSVNYFLRDEQGRYIAEKVDKRVWLKWMELRVHGDVDAVRTPTGLIPVYQDLVKLFEEH
LGKDYPESRYEKEFMLRVPQHLAKIERVWKVYEQIPDTPKVLFDVLRKQKERLKEAQSRWGDYISPFKFDRR
>Mature_631_residues
PRTRLLDEEYDYMAVLEARMSRDMLEKLLRIRDPELHRWIADVIRVTEPAMVYLITSEEDFEYVRKAALRNREELPTRYP
RHTVHFDGPRDIARDRKNTRILLPGGEKIPFINTYDRDKGLAEIRELLRGIMRGREMFIGFYCFGPKRSPFTLHAVQITD
SAYVAHNENILYRLCYDEFVEKAPNLFYARFIHSAGERGENGWSKNIDKRRIYVDLHDYTVYSVNTQYGGNTIGLKKLMF
RLCIYKGYLEGWLCEHMFIVGVRGPGGRLTYFTGAFPAGCGKTSTAFISDTIVGDDLAIIKPVNGVPHGVNPEMGMFGII
DGVNPRDDPVLYKLLTDPSNEIIFSNVLLTDDGEVWWNGKEGEPRPGLNYAGRWWPGKKDENGREVPPSHPNARFTISLR
SLPNLDPRVDDPMGVPISAMIFGGRDPDTWVPVEEAFDWVHGIITKAAALESEKTAAILEKAGVRELNPFAILDFLPISI
GKFIQLHLDFAEKVEKLPRIFSVNYFLRDEQGRYIAEKVDKRVWLKWMELRVHGDVDAVRTPTGLIPVYQDLVKLFEEHL
GKDYPESRYEKEFMLRVPQHLAKIERVWKVYEQIPDTPKVLFDVLRKQKERLKEAQSRWGDYISPFKFDRR

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI66346721, Length=546, Percent_Identity=33.3333333333333, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI187281517, Length=502, Percent_Identity=31.0756972111554, Blast_Score=220, Evalue=4e-57,
Organism=Homo sapiens, GI66346723, Length=388, Percent_Identity=30.4123711340206, Blast_Score=140, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17555492, Length=588, Percent_Identity=28.2312925170068, Blast_Score=227, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI32564411, Length=556, Percent_Identity=28.5971223021583, Blast_Score=223, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI71989658, Length=589, Percent_Identity=28.3531409168082, Blast_Score=219, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI71989645, Length=589, Percent_Identity=28.3531409168082, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI32564407, Length=517, Percent_Identity=29.4003868471954, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI71989653, Length=490, Percent_Identity=30.6122448979592, Blast_Score=214, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI71989649, Length=509, Percent_Identity=29.2730844793713, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI32564409, Length=342, Percent_Identity=30.4093567251462, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24655085, Length=593, Percent_Identity=29.8482293423271, Blast_Score=238, Evalue=9e-63,
Organism=Drosophila melanogaster, GI24655088, Length=495, Percent_Identity=31.5151515151515, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24655082, Length=629, Percent_Identity=29.570747217806, Blast_Score=231, Evalue=8e-61,
Organism=Drosophila melanogaster, GI161077226, Length=568, Percent_Identity=30.6338028169014, Blast_Score=226, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 73182; Mature: 73051

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRTRLLDEEYDYMAVLEARMSRDMLEKLLRIRDPELHRWIADVIRVTEPAMVYLITSEE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCH
DFEYVRKAALRNREELPTRYPRHTVHFDGPRDIARDRKNTRILLPGGEKIPFINTYDRDK
HHHHHHHHHHCCHHHCCCCCCCCEEECCCCHHHHCCCCCCEEEECCCCCCCEECCCCCCC
GLAEIRELLRGIMRGREMFIGFYCFGPKRSPFTLHAVQITDSAYVAHNENILYRLCYDEF
CHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEEEEECCEEEEECCCEEEEEHHHHH
VEKAPNLFYARFIHSAGERGENGWSKNIDKRRIYVDLHDYTVYSVNTQYGGNTIGLKKLM
HHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECEEEEEEECCCCCCCHHHHHHH
FRLCIYKGYLEGWLCEHMFIVGVRGPGGRLTYFTGAFPAGCGKTSTAFISDTIVGDDLAI
HHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCEEEHEECCCCEEE
IKPVNGVPHGVNPEMGMFGIIDGVNPRDDPVLYKLLTDPSNEIIFSNVLLTDDGEVWWNG
EECCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCEEEEEEEEECCCCEEECC
KEGEPRPGLNYAGRWWPGKKDENGREVPPSHPNARFTISLRSLPNLDPRVDDPMGVPISA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEE
MIFGGRDPDTWVPVEEAFDWVHGIITKAAALESEKTAAILEKAGVRELNPFAILDFLPIS
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
IGKFIQLHLDFAEKVEKLPRIFSVNYFLRDEQGRYIAEKVDKRVWLKWMELRVHGDVDAV
HHHHHEEEECHHHHHHHCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCHH
RTPTGLIPVYQDLVKLFEEHLGKDYPESRYEKEFMLRVPQHLAKIERVWKVYEQIPDTPK
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VLFDVLRKQKERLKEAQSRWGDYISPFKFDRR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PRTRLLDEEYDYMAVLEARMSRDMLEKLLRIRDPELHRWIADVIRVTEPAMVYLITSEE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCH
DFEYVRKAALRNREELPTRYPRHTVHFDGPRDIARDRKNTRILLPGGEKIPFINTYDRDK
HHHHHHHHHHCCHHHCCCCCCCCEEECCCCHHHHCCCCCCEEEECCCCCCCEECCCCCCC
GLAEIRELLRGIMRGREMFIGFYCFGPKRSPFTLHAVQITDSAYVAHNENILYRLCYDEF
CHHHHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEEEEEECCEEEEECCCEEEEEHHHHH
VEKAPNLFYARFIHSAGERGENGWSKNIDKRRIYVDLHDYTVYSVNTQYGGNTIGLKKLM
HHHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECEEEEEEECCCCCCCHHHHHHH
FRLCIYKGYLEGWLCEHMFIVGVRGPGGRLTYFTGAFPAGCGKTSTAFISDTIVGDDLAI
HHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCEEEHEECCCCEEE
IKPVNGVPHGVNPEMGMFGIIDGVNPRDDPVLYKLLTDPSNEIIFSNVLLTDDGEVWWNG
EECCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCEEEEEEEEECCCCEEECC
KEGEPRPGLNYAGRWWPGKKDENGREVPPSHPNARFTISLRSLPNLDPRVDDPMGVPISA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEE
MIFGGRDPDTWVPVEEAFDWVHGIITKAAALESEKTAAILEKAGVRELNPFAILDFLPIS
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
IGKFIQLHLDFAEKVEKLPRIFSVNYFLRDEQGRYIAEKVDKRVWLKWMELRVHGDVDAV
HHHHHEEEECHHHHHHHCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCHH
RTPTGLIPVYQDLVKLFEEHLGKDYPESRYEKEFMLRVPQHLAKIERVWKVYEQIPDTPK
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VLFDVLRKQKERLKEAQSRWGDYISPFKFDRR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA