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The map label for this gene is hemL [H]

Identifier: 124028279

GI number: 124028279

Start: 1435969

End: 1437354

Strand: Reverse

Name: hemL [H]

Synonym: Hbut_1430

Alternate gene names: 124028279

Gene position: 1437354-1435969 (Counterclockwise)

Preceding gene: 124028280

Following gene: 124028278

Centisome position: 86.22

GC content: 56.93

Gene sequence:

>1386_bases
ATGGTGCAGCTTGAGGAGAAGCTGGAAGAATGGTGGGCAAGACTGGCATCAGAATATGAGAAGCGGACGCCCCGCTCGAG
AGAACTATTTGAGAGGGCTAGGAGGGTCCTACCCGGGGGTACAACGTACCACATAAGGTTCTTCAAGCCATACCCAGTCT
TCATCGAGCATGGTCTAGGCCCAAGGGTTTGGGATGTAGACGGCAACGAATACACTGACTACTGGATGGGGCATGGAGCC
CTCATACTAGGCCATTGCCCGGACCTCCTCGAGGAGGCTGTACGCAAGGCTCTTAAGGCTAGCAGTCACCTAGGCTACGA
GAACCCCTACGCGCTGGAGTACGCCGAGCTTCTAGTCCAGGTACTCCCGGGTGTCGAGCAGGTACGCTTTACGAATAGTG
GCACCGAGGCGAACATGTACGCGGTGAGGCTCGCCAGGGCCTACACTGGGCGCAAGTACATAATAAAGCTCGAAGGCGCA
TGGCACGGTGGCTACGACGCGCTACACGTAGGCGTAACCCCTCCCTACGAGGGGCCGGAGTCCCTGGGTCTACCAGAGGA
GAGCATCAAGTACACCCTAGTAGCGCCATTCAACGATGCCGGGGCTGTGGAAAGGCTGGTGAAACGCTACGAGGTAGCCG
CAATATGGGTTGAACCGGTGCTCGGTGCTGGCGGTGGAATAGAGCCGGAGCCTGGCTACTTACGGGAGCTCCGGAGGATA
GCTGACGAAGCCGGAGCACTTCTCGTATTCGACGAGGTTATCACGGGGTTCCGCCTAGCCCTCGGAGGCGCCCAGGAATA
CTACAACGTTAAGGCAGACATAGTTGTGCTAGGCAAAATTGTTGGCGGCGGTATGCCAGGTGCGGGTGCGATAGCCTCGC
GAGCCGAGGTTATGGAGCTGCTGGATCAGCTTAAGAGGCCAAAGGGCCGGGAGAGGAGCTTCCACGGGGGCACCTTCACC
GGCAACCCGGTAACGATCCTCACAGGCTACCACACCGTGAAGCATCTTGCTGATAACCGCGGCGTCTACGCCGAGTTCAA
CAGTCTATGGGATAGTGTGAGGAGGAGGATCCAGGAGGAGTGTGAGTCCAGGGACAACATCTGCTGGGCAACCGGCACCG
GAAGCATAGTTGGGATACACTTCACCCGCAAGAGGCCGCGCACGGCGAGGGAAGCCCTGGAGGAGAGGTGGAGTAACAAG
GTATACGAGGTAATGCACATGTACATGAGGCTCAACGGTATACTTTACATGACCGAGCACATGGCACACTTGCTGCCATC
AATGGCTCATAGCCGCCGGGATGCTGACAGGTTTATAGAAGTGTTCGCGAGCTTCCTAGACGAGATCTACAGACTAGTTA
AAAAGGGAGCACTCTCCACATTTTAA

Upstream 100 bases:

>100_bases
GCCAGGCCTACTCACGATGGCGGACCTGGTACCCTTCAAGCCAAAGTTTGCTGTGCAGGTGCGCCGTTAGACCTCTCTGC
GCGGGCTTGGTGAGTGCTCT

Downstream 100 bases:

>100_bases
GCTGTAGAATCTGACGCCGCTGGGAATGATTAGGCCTTGGTTGAGGAGGGCACTGTAAGGGAGCGAATCATAAAGATCTT
GATGGAGGCTCGTCGCCCTC

Product: glutamate-1-semialdehyde 2,1-aminomutase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MVQLEEKLEEWWARLASEYEKRTPRSRELFERARRVLPGGTTYHIRFFKPYPVFIEHGLGPRVWDVDGNEYTDYWMGHGA
LILGHCPDLLEEAVRKALKASSHLGYENPYALEYAELLVQVLPGVEQVRFTNSGTEANMYAVRLARAYTGRKYIIKLEGA
WHGGYDALHVGVTPPYEGPESLGLPEESIKYTLVAPFNDAGAVERLVKRYEVAAIWVEPVLGAGGGIEPEPGYLRELRRI
ADEAGALLVFDEVITGFRLALGGAQEYYNVKADIVVLGKIVGGGMPGAGAIASRAEVMELLDQLKRPKGRERSFHGGTFT
GNPVTILTGYHTVKHLADNRGVYAEFNSLWDSVRRRIQEECESRDNICWATGTGSIVGIHFTRKRPRTAREALEERWSNK
VYEVMHMYMRLNGILYMTEHMAHLLPSMAHSRRDADRFIEVFASFLDEIYRLVKKGALSTF

Sequences:

>Translated_461_residues
MVQLEEKLEEWWARLASEYEKRTPRSRELFERARRVLPGGTTYHIRFFKPYPVFIEHGLGPRVWDVDGNEYTDYWMGHGA
LILGHCPDLLEEAVRKALKASSHLGYENPYALEYAELLVQVLPGVEQVRFTNSGTEANMYAVRLARAYTGRKYIIKLEGA
WHGGYDALHVGVTPPYEGPESLGLPEESIKYTLVAPFNDAGAVERLVKRYEVAAIWVEPVLGAGGGIEPEPGYLRELRRI
ADEAGALLVFDEVITGFRLALGGAQEYYNVKADIVVLGKIVGGGMPGAGAIASRAEVMELLDQLKRPKGRERSFHGGTFT
GNPVTILTGYHTVKHLADNRGVYAEFNSLWDSVRRRIQEECESRDNICWATGTGSIVGIHFTRKRPRTAREALEERWSNK
VYEVMHMYMRLNGILYMTEHMAHLLPSMAHSRRDADRFIEVFASFLDEIYRLVKKGALSTF
>Mature_461_residues
MVQLEEKLEEWWARLASEYEKRTPRSRELFERARRVLPGGTTYHIRFFKPYPVFIEHGLGPRVWDVDGNEYTDYWMGHGA
LILGHCPDLLEEAVRKALKASSHLGYENPYALEYAELLVQVLPGVEQVRFTNSGTEANMYAVRLARAYTGRKYIIKLEGA
WHGGYDALHVGVTPPYEGPESLGLPEESIKYTLVAPFNDAGAVERLVKRYEVAAIWVEPVLGAGGGIEPEPGYLRELRRI
ADEAGALLVFDEVITGFRLALGGAQEYYNVKADIVVLGKIVGGGMPGAGAIASRAEVMELLDQLKRPKGRERSFHGGTFT
GNPVTILTGYHTVKHLADNRGVYAEFNSLWDSVRRRIQEECESRDNICWATGTGSIVGIHFTRKRPRTAREALEERWSNK
VYEVMHMYMRLNGILYMTEHMAHLLPSMAHSRRDADRFIEVFASFLDEIYRLVKKGALSTF

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=317, Percent_Identity=32.1766561514196, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI13994255, Length=442, Percent_Identity=24.6606334841629, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI24119277, Length=325, Percent_Identity=29.5384615384615, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI37574042, Length=381, Percent_Identity=25.4593175853018, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI226442709, Length=345, Percent_Identity=25.7971014492754, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI226442705, Length=382, Percent_Identity=24.869109947644, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI284507298, Length=206, Percent_Identity=32.5242718446602, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1786349, Length=424, Percent_Identity=35.6132075471698, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI1789016, Length=376, Percent_Identity=30.5851063829787, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789759, Length=280, Percent_Identity=34.2857142857143, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI145693181, Length=333, Percent_Identity=31.8318318318318, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787560, Length=395, Percent_Identity=29.1139240506329, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1788044, Length=311, Percent_Identity=30.8681672025724, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1786991, Length=299, Percent_Identity=29.4314381270903, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI25144271, Length=329, Percent_Identity=28.8753799392097, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71992977, Length=357, Percent_Identity=27.4509803921569, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI32564660, Length=342, Percent_Identity=26.3157894736842, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI25144274, Length=148, Percent_Identity=32.4324324324324, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323470, Length=441, Percent_Identity=24.2630385487528, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324432, Length=391, Percent_Identity=25.8312020460358, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21357415, Length=324, Percent_Identity=29.6296296296296, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356575, Length=445, Percent_Identity=25.6179775280899, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161085790, Length=321, Percent_Identity=28.9719626168224, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28574759, Length=321, Percent_Identity=28.9719626168224, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 52113; Mature: 52113

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQLEEKLEEWWARLASEYEKRTPRSRELFERARRVLPGGTTYHIRFFKPYPVFIEHGLG
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHEECCCC
PRVWDVDGNEYTDYWMGHGALILGHCPDLLEEAVRKALKASSHLGYENPYALEYAELLVQ
CEEEECCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VLPGVEQVRFTNSGTEANMYAVRLARAYTGRKYIIKLEGAWHGGYDALHVGVTPPYEGPE
HHCCHHHEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCCCCCCCC
SLGLPEESIKYTLVAPFNDAGAVERLVKRYEVAAIWVEPVLGAGGGIEPEPGYLRELRRI
CCCCCHHHCEEEEEECCCCHHHHHHHHHHHHHHHEEEEHHHCCCCCCCCCCHHHHHHHHH
ADEAGALLVFDEVITGFRLALGGAQEYYNVKADIVVLGKIVGGGMPGAGAIASRAEVMEL
HHHCCEEEEHHHHHHHHHHHHCCHHHHHCCEEHHEEEEHHHCCCCCCCCHHHHHHHHHHH
LDQLKRPKGRERSFHGGTFTGNPVTILTGYHTVKHLADNRGVYAEFNSLWDSVRRRIQEE
HHHHHCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHH
CESRDNICWATGTGSIVGIHFTRKRPRTAREALEERWSNKVYEVMHMYMRLNGILYMTEH
HCCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
MAHLLPSMAHSRRDADRFIEVFASFLDEIYRLVKKGALSTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MVQLEEKLEEWWARLASEYEKRTPRSRELFERARRVLPGGTTYHIRFFKPYPVFIEHGLG
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHEECCCC
PRVWDVDGNEYTDYWMGHGALILGHCPDLLEEAVRKALKASSHLGYENPYALEYAELLVQ
CEEEECCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VLPGVEQVRFTNSGTEANMYAVRLARAYTGRKYIIKLEGAWHGGYDALHVGVTPPYEGPE
HHCCHHHEEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCCCCCCCC
SLGLPEESIKYTLVAPFNDAGAVERLVKRYEVAAIWVEPVLGAGGGIEPEPGYLRELRRI
CCCCCHHHCEEEEEECCCCHHHHHHHHHHHHHHHEEEEHHHCCCCCCCCCCHHHHHHHHH
ADEAGALLVFDEVITGFRLALGGAQEYYNVKADIVVLGKIVGGGMPGAGAIASRAEVMEL
HHHCCEEEEHHHHHHHHHHHHCCHHHHHCCEEHHEEEEHHHCCCCCCCCHHHHHHHHHHH
LDQLKRPKGRERSFHGGTFTGNPVTILTGYHTVKHLADNRGVYAEFNSLWDSVRRRIQEE
HHHHHCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHH
CESRDNICWATGTGSIVGIHFTRKRPRTAREALEERWSNKVYEVMHMYMRLNGILYMTEH
HCCCCCEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
MAHLLPSMAHSRRDADRFIEVFASFLDEIYRLVKKGALSTF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]