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The map label for this gene is metG

Identifier: 124027978

GI number: 124027978

Start: 1124839

End: 1126557

Strand: Reverse

Name: metG

Synonym: Hbut_1110

Alternate gene names: 124027978

Gene position: 1126557-1124839 (Counterclockwise)

Preceding gene: 124027982

Following gene: 124027977

Centisome position: 67.57

GC content: 49.85

Gene sequence:

>1719_bases
ATGGGTAGGTGGATAGTAGCCTCTGCATGGCCATATGTTAACAGCATTCCACATCTCGGCAACCTCATAGGCTCCATACT
CTCTGCCGACGTCTTCGCCCGCTACCTGCGCCTCAAGGGCGAAGACGTAGTATTCGTTAGCGGTAGTGATGAACATGGCA
CTCCAATAGAGGTTGAAGCCATAAGGAGGGGTGTACACCCCAAGCAGCTCACCGACCAAGCACACGAGTACGTGAAGAAG
CTCTTTGAAGAATTTAGGATAAGCTTTGACAATTACACGCGCACCGAGAACCCTGTACACAAGGAGTTTGTGAGAGAATT
CATGATGAAGCTCTACCAGAACGGCTACATATTCGAGCAGGACGACGTACTACCCTACTGTCCACGCGACAAAATGTTCC
TCCCAGACCGCTTTGTCGTAGGTACATGTCCATACTGTGGCTTCGAGAAAGCCTATGGGGACCAGTGCGACAACTGTGGC
AGACTACTTCACCCCACAGAGCTCAAAAACCCACGTTGCTCAATATGTGGTGGCCCCGTAGAGTTCCGCAAGAGTAAGCA
TTGGTTTTTCAATCTTCCAAAACTCCAAGAGAAGGTTGAGAAGTGGCTCCGCGAGTCAAATCTCCCACCAAACGTCAAAA
ACTACAGCCTTAACATGCTTAAGGAAGGTCTCAAGCCTAGGAGCGTAACAAGGGACAACAAGTGGGGGATTCCAGCCCCA
TTCCCCGGCGCCGAGGGCAAGACAATATACGTCTGGTTTGACGCGCTCCTAGGCTACATTTCAGCCACAAAAGAGTATGG
GCTGAAGAAGGGAGATCCCGAACTATGGAAGAAGTATTGGTTCAACCCCGAGACAAAGACCGTGTACTTCATAGGCAAGG
ACAACATACCTTTCCATGCAATAATCCTGCCAGCAATGCTAATCGGTAGCGGTGATCCATACGTACTACCAAGCTACATC
TCTGCTACAGAGTACTTGATGTACGAGGGCGAACAGTTCTCAAAGAGCCGCAGATGGGGCATCTGGATAGATGAGGCTCT
AGAGATACTTCCAGCTGACTATTGGCGGTTTGCCCTCATAAGGATGAGGCCTGAGGCTAAGGACACAAACTTTACTTGGA
GTGAGTTCTTGAGAATAGTTAATACCGAAATGAATGACGACATAGGCAACTACGTCCATCGAGTACTTAGATTCATAGAA
TCAAAGTTTGGCTCTGTAGTGCCAGAACCAGGCTCCTATAGCGATGCAGACAAGGAGTTCGAGAAGGCTATAAGAGAGAC
CCCGCGAAGGGCCGAGAAGTATTTCGAAGAAGTCAGACTCAAGCAGGCCCTAGGAGAAGTTATAGAGCTAGCCCGTAAGG
GCAACCAGTACTTGAACACCAAGGCACCCTGGGATAAGATAAAGACTGATCCAAAGGATGCAGCTACAACAATGTACCTC
GCAGTAAACTCTGTAGCTACTCTAGCGATACTCTTGGCGCCGTTCACGCCAGACTCTGCCGAGAGGCTCTGGAAGATGCT
AAATCTTCCAGGTAGCGTGCATGAGCCAGGGCGCTGGAAGCAGGCTGGTGAGATGATAATAAAGCCAGGCCACAGGATAG
GTAAGCCAGAACAGCTCTTCCGCAAGCTCCCAGCACGCTTCGACAAGGAGATAGCAAGTAAGCTACAGGAGATAAGGAGA
AAAGTCATGGAGAAGAGGCCGCCGTTACTACGCTGGTAG

Upstream 100 bases:

>100_bases
TAGACTGGTACCGGGCAGAGTTAATAAAGAGCCAAAACAAGCCGCGCTAGAGATAGGTAGCACATAGCCCCTTCCAGGGT
AGTAGTAGCGGTGAGTTGTT

Downstream 100 bases:

>100_bases
GAATTGTGGGGCACGGAACTGTTTTTGCCCCAGCCTAGGCTCAGCTCAACATTAAGTCCTCGCCTCGGGGCAGTGTTAAG
GTGCCGCGCCGGCTACGGAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MGRWIVASAWPYVNSIPHLGNLIGSILSADVFARYLRLKGEDVVFVSGSDEHGTPIEVEAIRRGVHPKQLTDQAHEYVKK
LFEEFRISFDNYTRTENPVHKEFVREFMMKLYQNGYIFEQDDVLPYCPRDKMFLPDRFVVGTCPYCGFEKAYGDQCDNCG
RLLHPTELKNPRCSICGGPVEFRKSKHWFFNLPKLQEKVEKWLRESNLPPNVKNYSLNMLKEGLKPRSVTRDNKWGIPAP
FPGAEGKTIYVWFDALLGYISATKEYGLKKGDPELWKKYWFNPETKTVYFIGKDNIPFHAIILPAMLIGSGDPYVLPSYI
SATEYLMYEGEQFSKSRRWGIWIDEALEILPADYWRFALIRMRPEAKDTNFTWSEFLRIVNTEMNDDIGNYVHRVLRFIE
SKFGSVVPEPGSYSDADKEFEKAIRETPRRAEKYFEEVRLKQALGEVIELARKGNQYLNTKAPWDKIKTDPKDAATTMYL
AVNSVATLAILLAPFTPDSAERLWKMLNLPGSVHEPGRWKQAGEMIIKPGHRIGKPEQLFRKLPARFDKEIASKLQEIRR
KVMEKRPPLLRW

Sequences:

>Translated_572_residues
MGRWIVASAWPYVNSIPHLGNLIGSILSADVFARYLRLKGEDVVFVSGSDEHGTPIEVEAIRRGVHPKQLTDQAHEYVKK
LFEEFRISFDNYTRTENPVHKEFVREFMMKLYQNGYIFEQDDVLPYCPRDKMFLPDRFVVGTCPYCGFEKAYGDQCDNCG
RLLHPTELKNPRCSICGGPVEFRKSKHWFFNLPKLQEKVEKWLRESNLPPNVKNYSLNMLKEGLKPRSVTRDNKWGIPAP
FPGAEGKTIYVWFDALLGYISATKEYGLKKGDPELWKKYWFNPETKTVYFIGKDNIPFHAIILPAMLIGSGDPYVLPSYI
SATEYLMYEGEQFSKSRRWGIWIDEALEILPADYWRFALIRMRPEAKDTNFTWSEFLRIVNTEMNDDIGNYVHRVLRFIE
SKFGSVVPEPGSYSDADKEFEKAIRETPRRAEKYFEEVRLKQALGEVIELARKGNQYLNTKAPWDKIKTDPKDAATTMYL
AVNSVATLAILLAPFTPDSAERLWKMLNLPGSVHEPGRWKQAGEMIIKPGHRIGKPEQLFRKLPARFDKEIASKLQEIRR
KVMEKRPPLLRW
>Mature_571_residues
GRWIVASAWPYVNSIPHLGNLIGSILSADVFARYLRLKGEDVVFVSGSDEHGTPIEVEAIRRGVHPKQLTDQAHEYVKKL
FEEFRISFDNYTRTENPVHKEFVREFMMKLYQNGYIFEQDDVLPYCPRDKMFLPDRFVVGTCPYCGFEKAYGDQCDNCGR
LLHPTELKNPRCSICGGPVEFRKSKHWFFNLPKLQEKVEKWLRESNLPPNVKNYSLNMLKEGLKPRSVTRDNKWGIPAPF
PGAEGKTIYVWFDALLGYISATKEYGLKKGDPELWKKYWFNPETKTVYFIGKDNIPFHAIILPAMLIGSGDPYVLPSYIS
ATEYLMYEGEQFSKSRRWGIWIDEALEILPADYWRFALIRMRPEAKDTNFTWSEFLRIVNTEMNDDIGNYVHRVLRFIES
KFGSVVPEPGSYSDADKEFEKAIRETPRRAEKYFEEVRLKQALGEVIELARKGNQYLNTKAPWDKIKTDPKDAATTMYLA
VNSVATLAILLAPFTPDSAERLWKMLNLPGSVHEPGRWKQAGEMIIKPGHRIGKPEQLFRKLPARFDKEIASKLQEIRRK
VMEKRPPLLRW

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily

Homologues:

Organism=Homo sapiens, GI14043022, Length=550, Percent_Identity=38.7272727272727, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI24308436, Length=530, Percent_Identity=28.1132075471698, Blast_Score=191, Evalue=1e-48,
Organism=Escherichia coli, GI1788432, Length=548, Percent_Identity=30.8394160583942, Blast_Score=270, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17540794, Length=556, Percent_Identity=40.2877697841727, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI212645096, Length=524, Percent_Identity=28.2442748091603, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71992781, Length=419, Percent_Identity=26.2529832935561, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321704, Length=552, Percent_Identity=36.7753623188406, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321610, Length=543, Percent_Identity=24.8618784530387, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323414, Length=342, Percent_Identity=23.6842105263158, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24655311, Length=568, Percent_Identity=38.556338028169, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI21358483, Length=524, Percent_Identity=26.5267175572519, Blast_Score=166, Evalue=4e-41,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_HYPBU (A2BLU2)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013298.1
- STRING:   A2BLU2
- GeneID:   4782382
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_1110
- eggNOG:   arNOG04371
- HOGENOM:   HBG721667
- OMA:   WGIWIDE
- PhylomeDB:   A2BLU2
- ProtClustDB:   PRK12268
- BioCyc:   HBUT415426:HBUT_1110-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 66433; Mature: 66302

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRWIVASAWPYVNSIPHLGNLIGSILSADVFARYLRLKGEDVVFVSGSDEHGTPIEVEA
CCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHHH
IRRGVHPKQLTDQAHEYVKKLFEEFRISFDNYTRTENPVHKEFVREFMMKLYQNGYIFEQ
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECC
DDVLPYCPRDKMFLPDRFVVGTCPYCGFEKAYGDQCDNCGRLLHPTELKNPRCSICGGPV
CCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCH
EFRKSKHWFFNLPKLQEKVEKWLRESNLPPNVKNYSLNMLKEGLKPRSVTRDNKWGIPAP
HHCCCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCC
FPGAEGKTIYVWFDALLGYISATKEYGLKKGDPELWKKYWFNPETKTVYFIGKDNIPFHA
CCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCHHH
IILPAMLIGSGDPYVLPSYISATEYLMYEGEQFSKSRRWGIWIDEALEILPADYWRFALI
HHHHHHHHCCCCCEECHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHCCCHHHHEEHH
RMRPEAKDTNFTWSEFLRIVNTEMNDDIGNYVHRVLRFIESKFGSVVPEPGSYSDADKEF
EECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
EKAIRETPRRAEKYFEEVRLKQALGEVIELARKGNQYLNTKAPWDKIKTDPKDAATTMYL
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHH
AVNSVATLAILLAPFTPDSAERLWKMLNLPGSVHEPGRWKQAGEMIIKPGHRIGKPEQLF
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCEECCCCCCCCHHHHH
RKLPARFDKEIASKLQEIRRKVMEKRPPLLRW
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GRWIVASAWPYVNSIPHLGNLIGSILSADVFARYLRLKGEDVVFVSGSDEHGTPIEVEA
CCEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEHHH
IRRGVHPKQLTDQAHEYVKKLFEEFRISFDNYTRTENPVHKEFVREFMMKLYQNGYIFEQ
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECC
DDVLPYCPRDKMFLPDRFVVGTCPYCGFEKAYGDQCDNCGRLLHPTELKNPRCSICGGPV
CCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCH
EFRKSKHWFFNLPKLQEKVEKWLRESNLPPNVKNYSLNMLKEGLKPRSVTRDNKWGIPAP
HHCCCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCC
FPGAEGKTIYVWFDALLGYISATKEYGLKKGDPELWKKYWFNPETKTVYFIGKDNIPFHA
CCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCHHH
IILPAMLIGSGDPYVLPSYISATEYLMYEGEQFSKSRRWGIWIDEALEILPADYWRFALI
HHHHHHHHCCCCCEECHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHCCCHHHHEEHH
RMRPEAKDTNFTWSEFLRIVNTEMNDDIGNYVHRVLRFIESKFGSVVPEPGSYSDADKEF
EECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
EKAIRETPRRAEKYFEEVRLKQALGEVIELARKGNQYLNTKAPWDKIKTDPKDAATTMYL
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHH
AVNSVATLAILLAPFTPDSAERLWKMLNLPGSVHEPGRWKQAGEMIIKPGHRIGKPEQLF
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCEECCCCCCCCHHHHH
RKLPARFDKEIASKLQEIRRKVMEKRPPLLRW
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA