Click here to switch to the map view.

The map label for this gene is gspE [C]

Identifier: 124027967

GI number: 124027967

Start: 1113795

End: 1115462

Strand: Direct

Name: gspE [C]

Synonym: Hbut_1099

Alternate gene names: 124027967

Gene position: 1113795-1115462 (Clockwise)

Preceding gene: 124027955

Following gene: 124027968

Centisome position: 66.81

GC content: 49.94

Gene sequence:

>1668_bases
ATGTTTCTGGCGAAACTGCTCAGCAAGATTAAGCTGCGTAGGCATGAGGAGAAGAAGCCAGAGGCAGACTTTGAGGAAGA
GCTGGAGCCGCTAGATTATAGGAGGCGGACACTATCATCGGTATGTCCCCAAAAGGAGTGTGAGGAGCTGGAATCATATC
CCCTAGAGGATCCATGGGTGCACGCGATAATACTGAGGAATAAGCGTACCGGCGAGATCATATACTGGATAGACGAGATA
CCCCTCTCCCCCGAGGAGAAGAAGATCTACGACAACATAATGGACATACTGTACTGGGAGCTTAAGCCACCAACAAGAGA
AGACGTGGACGTCTTCGCATACTTTGCAAAGGAGGCTCGCAAGGTTGTAGAAGTCTTCCAGATTAGGCTTGGCCGAACAC
CCGGCATCTCATGGGGCAAGATCCTCTACTACGTTCTGCGTGACGCTGTAGGCTTCGGGCCCATAGACCCGCTGATGAAG
GATCCCAACATAGAGGATATATCTTGTAACGGTGTGGGTAGGAGGGTGTATGTGTGGCACCGCCGCTACGAGTATATACC
AACAAACCTCATCTTCCACTCATCCGAGGAGCTGGATAATCTCGTCGTAAAGCTTGCCCATATGGCTGGCAAGCATGTAT
CCGTTGCATTCCCTATAGTTGATGCTATATTGCCTGGCGGCCACCGTCTCGCAGCCACCTATAAGAAGGAGGTTACAACA
GGAGGCTCCACGTTCACCATACGTAAATTTAGGGAGGACCCAATAACAATTGTCGACATGATAAGGTTTAGGACTATAAG
CGCCGAACTCGCTGCATACTACTGGCTGCTCATGGAACACAAGATGCCTGGCATGGTGCTTGGCGTAACTGGTTCCGGTA
AGACCACAACGCTCAACGCCTTGTTAACACTGCTGAAGCCCTCGGTAAAAGTTGTAACTATTGAGGATACACCGGAACTC
AAGCTACCGCTTGAGAACTGGGTTCAGCTAATCCCAAGGATGAGCTATGGCCTTGGCATGGAAAAGATTGGAGAGATAAG
CCTCTACGACCTGGTAAGGGTTAGTCTGCGATATAGGCCCGACATAATCGTTGTCGGTGAGGTACGTGGCGAAGAGGCTT
ACGTGCTATTCCAAGCCATCGCAACAGGCCACGGCGGCATGACTACACTACATGCAGAGTCCATTGACGCCGCTGTTAAG
AGGCTTACAAGTCCACCAATGAATATCCCGGAGGGCTATATACCACTGATGAACTTTACACTACTAATACGTAGGGTCGA
AATCATTGATCCCCAAACCGGTAGGCCTAAGATCACTAGGAGGATTACCTATACTTGGGAGATAGCAGATTATGGTAAGT
ACATCGTGTCCCATAAGTGGGACGCGCGTAGGGATAAGCACATAGTAAAACTTAAGGACAGCGTGCTCATGAAGAGGATT
ATGGAGCTGCACGGCTGGGATAAGGAGTACTTGCTCCAGGAGTTCAATAGAAGAGCTACAGTCCTGAAGTGGATGGCAGC
TAAGAACATGAGGAGCTATGTTGAAGTTGCAAGGATTATACGCGAGTACTACATGGATCCGGAGGGCACCCACAAGATTG
CCATACGCGACCTAGGCGGGGAGGCTGAAGTAGAGGCTACAGAATACCAGCCTGGAAGCCTGATGTAG

Upstream 100 bases:

>100_bases
TTAGTGATTGACATGTGTCACGTTTAGCTTAAAGCCCTGGAGTAAAAAAGGTATTTAGAGGCCACGAGATATGGAATGAG
AGCTAGGAGGAAAAGGGTAG

Downstream 100 bases:

>100_bases
AGCTGTTGGCTACCGAGGGATGTAGCCATATGCACTACGTGGTTTATCCGGTAGTCTCGCTACATCTGACCTAACTGGCA
AGAAGCTAGCAGTGACACCC

Product: Type II/IV secretion system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MFLAKLLSKIKLRRHEEKKPEADFEEELEPLDYRRRTLSSVCPQKECEELESYPLEDPWVHAIILRNKRTGEIIYWIDEI
PLSPEEKKIYDNIMDILYWELKPPTREDVDVFAYFAKEARKVVEVFQIRLGRTPGISWGKILYYVLRDAVGFGPIDPLMK
DPNIEDISCNGVGRRVYVWHRRYEYIPTNLIFHSSEELDNLVVKLAHMAGKHVSVAFPIVDAILPGGHRLAATYKKEVTT
GGSTFTIRKFREDPITIVDMIRFRTISAELAAYYWLLMEHKMPGMVLGVTGSGKTTTLNALLTLLKPSVKVVTIEDTPEL
KLPLENWVQLIPRMSYGLGMEKIGEISLYDLVRVSLRYRPDIIVVGEVRGEEAYVLFQAIATGHGGMTTLHAESIDAAVK
RLTSPPMNIPEGYIPLMNFTLLIRRVEIIDPQTGRPKITRRITYTWEIADYGKYIVSHKWDARRDKHIVKLKDSVLMKRI
MELHGWDKEYLLQEFNRRATVLKWMAAKNMRSYVEVARIIREYYMDPEGTHKIAIRDLGGEAEVEATEYQPGSLM

Sequences:

>Translated_555_residues
MFLAKLLSKIKLRRHEEKKPEADFEEELEPLDYRRRTLSSVCPQKECEELESYPLEDPWVHAIILRNKRTGEIIYWIDEI
PLSPEEKKIYDNIMDILYWELKPPTREDVDVFAYFAKEARKVVEVFQIRLGRTPGISWGKILYYVLRDAVGFGPIDPLMK
DPNIEDISCNGVGRRVYVWHRRYEYIPTNLIFHSSEELDNLVVKLAHMAGKHVSVAFPIVDAILPGGHRLAATYKKEVTT
GGSTFTIRKFREDPITIVDMIRFRTISAELAAYYWLLMEHKMPGMVLGVTGSGKTTTLNALLTLLKPSVKVVTIEDTPEL
KLPLENWVQLIPRMSYGLGMEKIGEISLYDLVRVSLRYRPDIIVVGEVRGEEAYVLFQAIATGHGGMTTLHAESIDAAVK
RLTSPPMNIPEGYIPLMNFTLLIRRVEIIDPQTGRPKITRRITYTWEIADYGKYIVSHKWDARRDKHIVKLKDSVLMKRI
MELHGWDKEYLLQEFNRRATVLKWMAAKNMRSYVEVARIIREYYMDPEGTHKIAIRDLGGEAEVEATEYQPGSLM
>Mature_555_residues
MFLAKLLSKIKLRRHEEKKPEADFEEELEPLDYRRRTLSSVCPQKECEELESYPLEDPWVHAIILRNKRTGEIIYWIDEI
PLSPEEKKIYDNIMDILYWELKPPTREDVDVFAYFAKEARKVVEVFQIRLGRTPGISWGKILYYVLRDAVGFGPIDPLMK
DPNIEDISCNGVGRRVYVWHRRYEYIPTNLIFHSSEELDNLVVKLAHMAGKHVSVAFPIVDAILPGGHRLAATYKKEVTT
GGSTFTIRKFREDPITIVDMIRFRTISAELAAYYWLLMEHKMPGMVLGVTGSGKTTTLNALLTLLKPSVKVVTIEDTPEL
KLPLENWVQLIPRMSYGLGMEKIGEISLYDLVRVSLRYRPDIIVVGEVRGEEAYVLFQAIATGHGGMTTLHAESIDAAVK
RLTSPPMNIPEGYIPLMNFTLLIRRVEIIDPQTGRPKITRRITYTWEIADYGKYIVSHKWDARRDKHIVKLKDSVLMKRI
MELHGWDKEYLLQEFNRRATVLKWMAAKNMRSYVEVARIIREYYMDPEGTHKIAIRDLGGEAEVEATEYQPGSLM

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG0630

COG function: function code NU; Type IV secretory pathway, VirB11 components, and related ATPases involved in archaeal flagella biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=122, Percent_Identity=39.344262295082, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786296, Length=136, Percent_Identity=33.0882352941176, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 64159; Mature: 64159

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLAKLLSKIKLRRHEEKKPEADFEEELEPLDYRRRTLSSVCPQKECEELESYPLEDPWV
CCHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCE
HAIILRNKRTGEIIYWIDEIPLSPEEKKIYDNIMDILYWELKPPTREDVDVFAYFAKEAR
EEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHH
KVVEVFQIRLGRTPGISWGKILYYVLRDAVGFGPIDPLMKDPNIEDISCNGVGRRVYVWH
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEECCCCCCEEEEEE
RRYEYIPTNLIFHSSEELDNLVVKLAHMAGKHVSVAFPIVDAILPGGHRLAATYKKEVTT
EEEEECCCEEEEECHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCEEEHHHHHHHCC
GGSTFTIRKFREDPITIVDMIRFRTISAELAAYYWLLMEHKMPGMVLGVTGSGKTTTLNA
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
LLTLLKPSVKVVTIEDTPELKLPLENWVQLIPRMSYGLGMEKIGEISLYDLVRVSLRYRP
HHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHEECC
DIIVVGEVRGEEAYVLFQAIATGHGGMTTLHAESIDAAVKRLTSPPMNIPEGYIPLMNFT
CEEEEEECCCCCCEEEEEHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
LLIRRVEIIDPQTGRPKITRRITYTWEIADYGKYIVSHKWDARRDKHIVKLKDSVLMKRI
HHHHHHHEECCCCCCCCEEEEEEEEEEEHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHH
MELHGWDKEYLLQEFNRRATVLKWMAAKNMRSYVEVARIIREYYMDPEGTHKIAIRDLGG
HHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCC
EAEVEATEYQPGSLM
CCCEEEECCCCCCCC
>Mature Secondary Structure
MFLAKLLSKIKLRRHEEKKPEADFEEELEPLDYRRRTLSSVCPQKECEELESYPLEDPWV
CCHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCE
HAIILRNKRTGEIIYWIDEIPLSPEEKKIYDNIMDILYWELKPPTREDVDVFAYFAKEAR
EEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHH
KVVEVFQIRLGRTPGISWGKILYYVLRDAVGFGPIDPLMKDPNIEDISCNGVGRRVYVWH
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEECCCCCCEEEEEE
RRYEYIPTNLIFHSSEELDNLVVKLAHMAGKHVSVAFPIVDAILPGGHRLAATYKKEVTT
EEEEECCCEEEEECHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCEEEHHHHHHHCC
GGSTFTIRKFREDPITIVDMIRFRTISAELAAYYWLLMEHKMPGMVLGVTGSGKTTTLNA
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
LLTLLKPSVKVVTIEDTPELKLPLENWVQLIPRMSYGLGMEKIGEISLYDLVRVSLRYRP
HHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHEECC
DIIVVGEVRGEEAYVLFQAIATGHGGMTTLHAESIDAAVKRLTSPPMNIPEGYIPLMNFT
CEEEEEECCCCCCEEEEEHHHCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
LLIRRVEIIDPQTGRPKITRRITYTWEIADYGKYIVSHKWDARRDKHIVKLKDSVLMKRI
HHHHHHHEECCCCCCCCEEEEEEEEEEEHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHH
MELHGWDKEYLLQEFNRRATVLKWMAAKNMRSYVEVARIIREYYMDPEGTHKIAIRDLGG
HHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCC
EAEVEATEYQPGSLM
CCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]