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The map label for this gene is rfaG [C]

Identifier: 124027963

GI number: 124027963

Start: 1107495

End: 1108742

Strand: Reverse

Name: rfaG [C]

Synonym: Hbut_1095

Alternate gene names: 124027963

Gene position: 1108742-1107495 (Counterclockwise)

Preceding gene: 124027964

Following gene: 124027962

Centisome position: 66.5

GC content: 54.65

Gene sequence:

>1248_bases
TTGGACGCTCTTAAGGTTGCGTTGGCGTCAGACTGGTTCTACCCAAAGATAGGAGGCATCGAGACACATATACACGAGCT
TGCACTCCAACTCCTCGAGATGGGTCATGAGCCTCACGTTATAACCCACGACTATCGCTACATGAAGCCCTATCGTGATG
ATTTCCCTTATGCTGTGCACAGGATTAAGGGTTTCTTCTACTTTCGCAAGAGCCATGTAAGCCTCGGAGTGGATACGCTC
TTCAAGCTCAACCGGCTCTACAAGACTATAGGCTTTGACATCACGCATATACACAGCATATATTCGCCATTCGCTGTTGC
AGCGGCCAATCTCTCCAGGGGTATCCGCGGCGTACCAGTAGTCGCTACCAACCACTCCCTCTACTACTGGTCTCCCGTCA
CTAAGCCCCTCATACCGCTCCTTCGTAATACGCTGAAACGGGTGGATGCATTCATAGCTGTTAGCCGCCGGGTAGCGGAG
GATACGAGGCGGCTCCTAGGCATAGTCGATGGCAAGGTTCCCCTCTACATTATCCCCAATGGGGTTAACACGGGGTTCTG
GAGGCCGCCAGAAGCTGAGGAGGAGAGGGCTGCCCGGAGAAAGCTTGGCCTAGATGAGCGCGAGTTTGTGGTCCTAGTGG
TTGGGAGGTTTACGGAGCGCAAACGGATACACCAAGCTCCTGCAATCGTGGCTTACGCTGCCAAGATAGTTGCTGGGAGG
AGGCGACTTCACCTCCTCATTATTGGTGATGGTCCGCTGCGCAGCAAGGTTCTACGAGCTGTTGAGGAGCACCTCTATGG
CCTGGATGGTGTAAGGGTAACCGTTCACGGTTTTATGGGTCGTGCTGGGCTGCGAGAGGCTTACTGGGCTTCGGATCTTC
TACTCGTGCCGGCGAGGCTGGAGGCATTCTCGATAGCAGCCCTCGAGGCCATGGCTTGTGGCAGACCAGTAATAGGGTTC
CGTGATGGCGGAATAGAGGACGTTGTAGCTGATGGTCGTACCGGGTTCCTTGTATCCTCTGATGAGGAGGCATCGGAGAG
GATAGCCGAGCTAGCCCTAGATCCTAACCTGCACCGTAGACTCGCAGAGACAGCCCCGCTACATGCTGCAGAGAACTTCT
CCTGGACGAGGATAGCCAGAAGCATCGTTGAGGTGTATAGGGCTACCATGGACGCGGCTATGGACAGCGATAAACGCTAC
CTCCTATACCGTGCCTGGCTCCGGGTATCAAGGATTCTTGGAAGGTGA

Upstream 100 bases:

>100_bases
GTACTGCACTAGCTATAGTCATGGTAGAGAGGCTTATTTCCTATGTATTTGCCTCGCTATTGGGCGGGCTGGCTGTTGCT
ATTGGTGGAGGCCGGCAAGC

Downstream 100 bases:

>100_bases
GCAGTCCGGTTTGCGCATAGCAGTACTCAGCTTTGACGTGGAGCAGGACTGTCCGCCATACCTCGGCGAGAGCTACCGCG
GGGTAGAGGAAGGGCTCCCA

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MDALKVALASDWFYPKIGGIETHIHELALQLLEMGHEPHVITHDYRYMKPYRDDFPYAVHRIKGFFYFRKSHVSLGVDTL
FKLNRLYKTIGFDITHIHSIYSPFAVAAANLSRGIRGVPVVATNHSLYYWSPVTKPLIPLLRNTLKRVDAFIAVSRRVAE
DTRRLLGIVDGKVPLYIIPNGVNTGFWRPPEAEEERAARRKLGLDEREFVVLVVGRFTERKRIHQAPAIVAYAAKIVAGR
RRLHLLIIGDGPLRSKVLRAVEEHLYGLDGVRVTVHGFMGRAGLREAYWASDLLLVPARLEAFSIAALEAMACGRPVIGF
RDGGIEDVVADGRTGFLVSSDEEASERIAELALDPNLHRRLAETAPLHAAENFSWTRIARSIVEVYRATMDAAMDSDKRY
LLYRAWLRVSRILGR

Sequences:

>Translated_415_residues
MDALKVALASDWFYPKIGGIETHIHELALQLLEMGHEPHVITHDYRYMKPYRDDFPYAVHRIKGFFYFRKSHVSLGVDTL
FKLNRLYKTIGFDITHIHSIYSPFAVAAANLSRGIRGVPVVATNHSLYYWSPVTKPLIPLLRNTLKRVDAFIAVSRRVAE
DTRRLLGIVDGKVPLYIIPNGVNTGFWRPPEAEEERAARRKLGLDEREFVVLVVGRFTERKRIHQAPAIVAYAAKIVAGR
RRLHLLIIGDGPLRSKVLRAVEEHLYGLDGVRVTVHGFMGRAGLREAYWASDLLLVPARLEAFSIAALEAMACGRPVIGF
RDGGIEDVVADGRTGFLVSSDEEASERIAELALDPNLHRRLAETAPLHAAENFSWTRIARSIVEVYRATMDAAMDSDKRY
LLYRAWLRVSRILGR
>Mature_415_residues
MDALKVALASDWFYPKIGGIETHIHELALQLLEMGHEPHVITHDYRYMKPYRDDFPYAVHRIKGFFYFRKSHVSLGVDTL
FKLNRLYKTIGFDITHIHSIYSPFAVAAANLSRGIRGVPVVATNHSLYYWSPVTKPLIPLLRNTLKRVDAFIAVSRRVAE
DTRRLLGIVDGKVPLYIIPNGVNTGFWRPPEAEEERAARRKLGLDEREFVVLVVGRFTERKRIHQAPAIVAYAAKIVAGR
RRLHLLIIGDGPLRSKVLRAVEEHLYGLDGVRVTVHGFMGRAGLREAYWASDLLLVPARLEAFSIAALEAMACGRPVIGF
RDGGIEDVVADGRTGFLVSSDEEASERIAELALDPNLHRRLAETAPLHAAENFSWTRIARSIVEVYRATMDAAMDSDKRY
LLYRAWLRVSRILGR

Specific function: Involved In The Addition Of The First Glucose Residue To The Lipopolysaccharide Core. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Homo sapiens, GI11863130, Length=410, Percent_Identity=26.3414634146341, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1790061, Length=182, Percent_Identity=31.8681318681319, Blast_Score=62, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17532709, Length=409, Percent_Identity=26.161369193154, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI9755344, Length=409, Percent_Identity=24.6943765281174, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24654571, Length=333, Percent_Identity=25.8258258258258, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 46939; Mature: 46939

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALKVALASDWFYPKIGGIETHIHELALQLLEMGHEPHVITHDYRYMKPYRDDFPYAVH
CCCCEEEHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCHHHCCCCCCCCHHHHH
RIKGFFYFRKSHVSLGVDTLFKLNRLYKTIGFDITHIHSIYSPFAVAAANLSRGIRGVPV
HHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEE
VATNHSLYYWSPVTKPLIPLLRNTLKRVDAFIAVSRRVAEDTRRLLGIVDGKVPLYIIPN
EEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
GVNTGFWRPPEAEEERAARRKLGLDEREFVVLVVGRFTERKRIHQAPAIVAYAAKIVAGR
CCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCHHHHHHHHHHHCC
RRLHLLIIGDGPLRSKVLRAVEEHLYGLDGVRVTVHGFMGRAGLREAYWASDLLLVPARL
CEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCHHHHHCCCEEEECCHH
EAFSIAALEAMACGRPVIGFRDGGIEDVVADGRTGFLVSSDEEASERIAELALDPNLHRR
HHHHHHHHHHHHCCCCCEECCCCCHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCHHHHH
LAETAPLHAAENFSWTRIARSIVEVYRATMDAAMDSDKRYLLYRAWLRVSRILGR
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDALKVALASDWFYPKIGGIETHIHELALQLLEMGHEPHVITHDYRYMKPYRDDFPYAVH
CCCCEEEHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCHHHCCCCCCCCHHHHH
RIKGFFYFRKSHVSLGVDTLFKLNRLYKTIGFDITHIHSIYSPFAVAAANLSRGIRGVPV
HHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEE
VATNHSLYYWSPVTKPLIPLLRNTLKRVDAFIAVSRRVAEDTRRLLGIVDGKVPLYIIPN
EEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
GVNTGFWRPPEAEEERAARRKLGLDEREFVVLVVGRFTERKRIHQAPAIVAYAAKIVAGR
CCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHCCHHHHHHHHHHHCC
RRLHLLIIGDGPLRSKVLRAVEEHLYGLDGVRVTVHGFMGRAGLREAYWASDLLLVPARL
CEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCHHHHHCCCEEEECCHH
EAFSIAALEAMACGRPVIGFRDGGIEDVVADGRTGFLVSSDEEASERIAELALDPNLHRR
HHHHHHHHHHHHCCCCCEECCCCCHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCHHHHH
LAETAPLHAAENFSWTRIARSIVEVYRATMDAAMDSDKRYLLYRAWLRVSRILGR
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Hexosyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]