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The map label for this gene is pcm

Identifier: 124027514

GI number: 124027514

Start: 608941

End: 609666

Strand: Direct

Name: pcm

Synonym: Hbut_0632

Alternate gene names: 124027514

Gene position: 608941-609666 (Clockwise)

Preceding gene: 124027513

Following gene: 124027515

Centisome position: 36.53

GC content: 57.02

Gene sequence:

>726_bases
TTGGCTCCATGGACTAGGGATGAGGAGTGGTTTAGAGAGGCTCGCCGCCGACTTGTAGAGAGGCTTGTCAGGGAGGGATA
CATAAGGTCGGAGCATGTGAAGAGGGCCATGCTTAGAGTGCCACGGGAGCTATTTGTGCCGGACGAGCTTCGCCACTTGG
CCTACGAGGATACCCCGCTACCAATAGGGCATGGGCAGACGATCAGTGCTCCCCACATGGTTGCAATGATGACAGAATAT
GCGGACCTAAAACCAGGTATGAAGGTTTTAGAGGTTGGCGCAGGCTCGGGCTACCATGCAGCAGTAATGGCGGAGGTTGT
AGCGCCTAGCGATGAGCCTCGAGAGCATTGGGGCCACGTATACACGATTGAGCGTATACCGGAGCTAGCAGAGTTCGCCC
GGAGAAACCTGGAGCGGGCAGGATATGCTGACCGCGTCACAGTCATAGTAGGCGATGGCTCGAGGGGCTACCCGGAGAAG
GCTCCCTACGACAGGATAATAGTGACAGCAGCGGCACCCGACATCCCCGGCCCACTCATAGACCAGCTCAAGCCGGGAGG
CAAAATGGTGATACCAATAGGCGACCGCTACCTCCAACACCTCTACGTGGTGGTGAAGACCAGGGACGGTAAGATAGAAT
CCAGACCGGTTACACCCTGCCTCTTCGTCCCACTAGTCGGGGAGTACGGCTGGAGAGAGTACGAAGCCGACACTACTCTA
GCCTAG

Upstream 100 bases:

>100_bases
AAACAATATCTACCCGTATTGGCTGTTTATTCAGAGAGGCTTGGCTGCGAGTGGAGTTATAGCCTATGCTCATGCAATAC
CTTGACTTAGGTGGCAGCGG

Downstream 100 bases:

>100_bases
CAATCCCGCTGCTCAGAGGCACTATACGCGGAAAGGAGGGCCACTGGAGAGGCCATGGTCTACACAAATCACGCCCCCTT
CCCGGCACGCGTCTAGGCTG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MAPWTRDEEWFREARRRLVERLVREGYIRSEHVKRAMLRVPRELFVPDELRHLAYEDTPLPIGHGQTISAPHMVAMMTEY
ADLKPGMKVLEVGAGSGYHAAVMAEVVAPSDEPREHWGHVYTIERIPELAEFARRNLERAGYADRVTVIVGDGSRGYPEK
APYDRIIVTAAAPDIPGPLIDQLKPGGKMVIPIGDRYLQHLYVVVKTRDGKIESRPVTPCLFVPLVGEYGWREYEADTTL
A

Sequences:

>Translated_241_residues
MAPWTRDEEWFREARRRLVERLVREGYIRSEHVKRAMLRVPRELFVPDELRHLAYEDTPLPIGHGQTISAPHMVAMMTEY
ADLKPGMKVLEVGAGSGYHAAVMAEVVAPSDEPREHWGHVYTIERIPELAEFARRNLERAGYADRVTVIVGDGSRGYPEK
APYDRIIVTAAAPDIPGPLIDQLKPGGKMVIPIGDRYLQHLYVVVKTRDGKIESRPVTPCLFVPLVGEYGWREYEADTTL
A
>Mature_240_residues
APWTRDEEWFREARRRLVERLVREGYIRSEHVKRAMLRVPRELFVPDELRHLAYEDTPLPIGHGQTISAPHMVAMMTEYA
DLKPGMKVLEVGAGSGYHAAVMAEVVAPSDEPREHWGHVYTIERIPELAEFARRNLERAGYADRVTVIVGDGSRGYPEKA
PYDRIIVTAAAPDIPGPLIDQLKPGGKMVIPIGDRYLQHLYVVVKTRDGKIESRPVTPCLFVPLVGEYGWREYEADTTLA

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=230, Percent_Identity=36.5217391304348, Blast_Score=124, Evalue=6e-29,
Organism=Homo sapiens, GI190360566, Length=225, Percent_Identity=27.5555555555556, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI157388995, Length=225, Percent_Identity=24.4444444444444, Blast_Score=71, Evalue=9e-13,
Organism=Escherichia coli, GI1789100, Length=210, Percent_Identity=42.3809523809524, Blast_Score=144, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71983477, Length=216, Percent_Identity=36.1111111111111, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI193207222, Length=214, Percent_Identity=34.1121495327103, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17981723, Length=216, Percent_Identity=36.5740740740741, Blast_Score=121, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_HYPBU (A2BKH8)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001012834.1
- ProteinModelPortal:   A2BKH8
- SMR:   A2BKH8
- STRING:   A2BKH8
- GeneID:   4781821
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0632
- eggNOG:   arNOG08853
- HOGENOM:   HBG699907
- OMA:   GYHAAVV
- PhylomeDB:   A2BKH8
- ProtClustDB:   PRK00312
- BioCyc:   HBUT415426:HBUT_0632-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 27311; Mature: 27180

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 69-69

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPWTRDEEWFREARRRLVERLVREGYIRSEHVKRAMLRVPRELFVPDELRHLAYEDTPL
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCCHHHHHHHCCCCCC
PIGHGQTISAPHMVAMMTEYADLKPGMKVLEVGAGSGYHAAVMAEVVAPSDEPREHWGHV
CCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHCCCE
YTIERIPELAEFARRNLERAGYADRVTVIVGDGSRGYPEKAPYDRIIVTAAAPDIPGPLI
EEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCHHH
DQLKPGGKMVIPIGDRYLQHLYVVVKTRDGKIESRPVTPCLFVPLVGEYGWREYEADTTL
HHCCCCCEEEEECCHHHHHHEEEEEECCCCCCCCCCCCCEEEEEHHCCCCCEEECCCCCC
A
C
>Mature Secondary Structure 
APWTRDEEWFREARRRLVERLVREGYIRSEHVKRAMLRVPRELFVPDELRHLAYEDTPL
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCCHHHHHHHCCCCCC
PIGHGQTISAPHMVAMMTEYADLKPGMKVLEVGAGSGYHAAVMAEVVAPSDEPREHWGHV
CCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCHHHCCCE
YTIERIPELAEFARRNLERAGYADRVTVIVGDGSRGYPEKAPYDRIIVTAAAPDIPGPLI
EEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCHHH
DQLKPGGKMVIPIGDRYLQHLYVVVKTRDGKIESRPVTPCLFVPLVGEYGWREYEADTTL
HHCCCCCEEEEECCHHHHHHEEEEEECCCCCCCCCCCCCEEEEEHHCCCCCEEECCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA