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The map label for this gene is gltX

Identifier: 124027427

GI number: 124027427

Start: 521248

End: 522984

Strand: Direct

Name: gltX

Synonym: Hbut_0542

Alternate gene names: 124027427

Gene position: 521248-522984 (Clockwise)

Preceding gene: 124027426

Following gene: 124027428

Centisome position: 31.27

GC content: 52.16

Gene sequence:

>1737_bases
TTGCTTAACGCTGTCGGACACGGTGGCAGGGCAGCGGTAGGCCCAGTAATGGGCAAGATAATGGCTGAAAGACCTGACCT
AAGGCCACAAGCCAAGCAGATAGTAGCAATTGTGCGTGAGGTCGTGGAGGAGGTTAACAAGCTGAGCCTCGAGGAGCAGA
AGAGGCTGCTGGAAGAGAAGTATAGCTGGGTTATCGAGAAGAAACTGCAGAAGCGGAGGGAGGCTGTGGAAAAGAAGCTA
CCGCCACTGCCTGATGCTGAGGAGGGTCGGGTGGTTACACGGTTCGCTCCAAACCCTGACTTCGTCATACATCTCGGCAA
TGCTAGACCCGCTCTCCTCTCCTACGAATACGCTGTAATGTATCGTGGCAAGATGATACTCCGCTTTGAGGATACCGATC
CGAGGATTAAGACGCCCCTACCCGAGGCATACGAGTTGATAAGGGAGGATCTCAAATGGCTCGGTATACGCTGGGACGAG
GAGTACATACAGAGTCTAAGGATGCACATCTACTATGACATAGCCAGGAAACTAATAGAGGTTGGTGGTGCGTACGTAGA
CGACCGCAGCCCGGAGGAGTTTAGGAGGTACCGAGACGAAGGCAGGCTAGAGGACTATCCGCCGCGCAAGCGCAGTGTCG
AGGAGAACCTCGAGCTATGGGACAAAATGGTCTCCGGAGCCTTTGGCGAAGGCGAAGCAGTACTAAGAGTAAAGACCGAC
CCCCGGCACCCTGATCCCAGCGTGCGTGACTGGGTAGCATTCCGCATCATAGACACCAGCCGCTATCCCCACCCCCTAGT
AGGCGACCGTTATGTAGCATGGCCGACCTACAACTTCGCAGCAGCAGTCGACGACAAGTTAATGGGTGTAACCCACATAC
TTCGTGCAAAAGAGCACATGCAGAACACAATTAAACAACAGTTCCTCTACAAGCACCTAGGCTGGAGCTATCCTCACGTA
GTACACTTTGGAAGGCTTAAGCTTGAAGGCTTCATAATGAGTAAGTCTACGCTAAAACGGTTTCTCGATGCAGGCATAAG
TAGGGGCATTGATGACCCACGCTTTGCAACGATTGCAGGGCTTAGACGTCGGGGGATTGTGCCGGAAGCTATAAAGAAGC
TAATAATGGAGGTCGGCGTAAAATATACCGATGCAAGTATAAGCTATGACAACTTAGCAGCGATAAACAGATCGATAATC
GATCCACGTGCCAAGAGGATAATGGCTGCATTGAGTCCTGTACCAGTAGAGGTTGAGGGATTGCCGTGGAGGGAGAAGGA
GTTCAGAATACCTTTCCATCCTTCAGGCCAGCTTGGCGAGAGAATAGTCAAGCTTGCCGGGCCTAAGGCAACCATATACG
TGTCCCAGAGCGATGCAGTGCAGCTTGCCAAGGGCAACATTGTGAGGCTAATGGAAGCCTTCAACATAGAGGTTCTAGGG
GCGTCACCTAGACGTGTAAGGGCACGCTATCACAGCTTAACAGTTGATGAGGCTAGGCAGCATAACGCGCCCATAATACA
GTGGGTGCCAGCCACGGATAACATTGCAGTTGAAGTGTTGAAGCCGGAAGGTCTAGACCTAGTACAGGAGCGTGGCCTCG
CAGAACCAGCAGCAGCCCAGCTGAAACCGGGCGAAATAATACAGCTCGTACGCATAGGATTTGCGCGGGTTGATAGCGTC
GAGACAGACGAAAAGGGGAAAGTAAGGAAGGTAGTGCTCATCTATGCTCATGACTGA

Upstream 100 bases:

>100_bases
CCACCAGACCGGAACATAGTGTTCATAGCGCCAGAGGAGGTGGTTCAGACTGTCACAGTACAATCCTGAGGAGATTAGAG
AGCTAGCTCGCGCTTATGCA

Downstream 100 bases:

>100_bases
GAAGAAGCTTTCATTCAAAGTGGGGCCGCTAGTTTTGTAAACCCTTCCAGGCCTAGAGCCCTAAGCGTACTATGGAGGGG
CGACGACCGAGCCCCGGGAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MLNAVGHGGRAAVGPVMGKIMAERPDLRPQAKQIVAIVREVVEEVNKLSLEEQKRLLEEKYSWVIEKKLQKRREAVEKKL
PPLPDAEEGRVVTRFAPNPDFVIHLGNARPALLSYEYAVMYRGKMILRFEDTDPRIKTPLPEAYELIREDLKWLGIRWDE
EYIQSLRMHIYYDIARKLIEVGGAYVDDRSPEEFRRYRDEGRLEDYPPRKRSVEENLELWDKMVSGAFGEGEAVLRVKTD
PRHPDPSVRDWVAFRIIDTSRYPHPLVGDRYVAWPTYNFAAAVDDKLMGVTHILRAKEHMQNTIKQQFLYKHLGWSYPHV
VHFGRLKLEGFIMSKSTLKRFLDAGISRGIDDPRFATIAGLRRRGIVPEAIKKLIMEVGVKYTDASISYDNLAAINRSII
DPRAKRIMAALSPVPVEVEGLPWREKEFRIPFHPSGQLGERIVKLAGPKATIYVSQSDAVQLAKGNIVRLMEAFNIEVLG
ASPRRVRARYHSLTVDEARQHNAPIIQWVPATDNIAVEVLKPEGLDLVQERGLAEPAAAQLKPGEIIQLVRIGFARVDSV
ETDEKGKVRKVVLIYAHD

Sequences:

>Translated_578_residues
MLNAVGHGGRAAVGPVMGKIMAERPDLRPQAKQIVAIVREVVEEVNKLSLEEQKRLLEEKYSWVIEKKLQKRREAVEKKL
PPLPDAEEGRVVTRFAPNPDFVIHLGNARPALLSYEYAVMYRGKMILRFEDTDPRIKTPLPEAYELIREDLKWLGIRWDE
EYIQSLRMHIYYDIARKLIEVGGAYVDDRSPEEFRRYRDEGRLEDYPPRKRSVEENLELWDKMVSGAFGEGEAVLRVKTD
PRHPDPSVRDWVAFRIIDTSRYPHPLVGDRYVAWPTYNFAAAVDDKLMGVTHILRAKEHMQNTIKQQFLYKHLGWSYPHV
VHFGRLKLEGFIMSKSTLKRFLDAGISRGIDDPRFATIAGLRRRGIVPEAIKKLIMEVGVKYTDASISYDNLAAINRSII
DPRAKRIMAALSPVPVEVEGLPWREKEFRIPFHPSGQLGERIVKLAGPKATIYVSQSDAVQLAKGNIVRLMEAFNIEVLG
ASPRRVRARYHSLTVDEARQHNAPIIQWVPATDNIAVEVLKPEGLDLVQERGLAEPAAAQLKPGEIIQLVRIGFARVDSV
ETDEKGKVRKVVLIYAHD
>Mature_578_residues
MLNAVGHGGRAAVGPVMGKIMAERPDLRPQAKQIVAIVREVVEEVNKLSLEEQKRLLEEKYSWVIEKKLQKRREAVEKKL
PPLPDAEEGRVVTRFAPNPDFVIHLGNARPALLSYEYAVMYRGKMILRFEDTDPRIKTPLPEAYELIREDLKWLGIRWDE
EYIQSLRMHIYYDIARKLIEVGGAYVDDRSPEEFRRYRDEGRLEDYPPRKRSVEENLELWDKMVSGAFGEGEAVLRVKTD
PRHPDPSVRDWVAFRIIDTSRYPHPLVGDRYVAWPTYNFAAAVDDKLMGVTHILRAKEHMQNTIKQQFLYKHLGWSYPHV
VHFGRLKLEGFIMSKSTLKRFLDAGISRGIDDPRFATIAGLRRRGIVPEAIKKLIMEVGVKYTDASISYDNLAAINRSII
DPRAKRIMAALSPVPVEVEGLPWREKEFRIPFHPSGQLGERIVKLAGPKATIYVSQSDAVQLAKGNIVRLMEAFNIEVLG
ASPRRVRARYHSLTVDEARQHNAPIIQWVPATDNIAVEVLKPEGLDLVQERGLAEPAAAQLKPGEIIQLVRIGFARVDSV
ETDEKGKVRKVVLIYAHD

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI62241042, Length=511, Percent_Identity=31.3111545988258, Blast_Score=219, Evalue=7e-57,
Organism=Homo sapiens, GI4826960, Length=357, Percent_Identity=31.0924369747899, Blast_Score=156, Evalue=6e-38,
Organism=Homo sapiens, GI134288884, Length=310, Percent_Identity=29.0322580645161, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1786895, Length=524, Percent_Identity=30.9160305343511, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1788743, Length=380, Percent_Identity=27.1052631578947, Blast_Score=108, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17511007, Length=508, Percent_Identity=30.1181102362205, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17543332, Length=335, Percent_Identity=31.3432835820896, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71993394, Length=382, Percent_Identity=27.2251308900524, Blast_Score=95, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321192, Length=539, Percent_Identity=29.4990723562152, Blast_Score=200, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6324742, Length=386, Percent_Identity=31.8652849740933, Blast_Score=194, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6324540, Length=309, Percent_Identity=32.0388349514563, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24649466, Length=509, Percent_Identity=31.6306483300589, Blast_Score=221, Evalue=9e-58,
Organism=Drosophila melanogaster, GI17864488, Length=374, Percent_Identity=30.4812834224599, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24665321, Length=341, Percent_Identity=31.3782991202346, Blast_Score=114, Evalue=3e-25,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_HYPBU (A2BK91)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001012747.1
- STRING:   A2BK91
- GeneID:   4782144
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0542
- eggNOG:   arNOG04636
- HOGENOM:   HBG334108
- OMA:   MRFAPNP
- PhylomeDB:   A2BK91
- ProtClustDB:   PRK04156
- BioCyc:   HBUT415426:HBUT_0542-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_A
- InterPro:   IPR004526
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR022888
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:2.40.240.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00463

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.17

Molecular weight: Translated: 66010; Mature: 66010

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNAVGHGGRAAVGPVMGKIMAERPDLRPQAKQIVAIVREVVEEVNKLSLEEQKRLLEEK
CCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YSWVIEKKLQKRREAVEKKLPPLPDAEEGRVVTRFAPNPDFVIHLGNARPALLSYEYAVM
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCEEEEEEEE
YRGKMILRFEDTDPRIKTPLPEAYELIREDLKWLGIRWDEEYIQSLRMHIYYDIARKLIE
ECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VGGAYVDDRSPEEFRRYRDEGRLEDYPPRKRSVEENLELWDKMVSGAFGEGEAVLRVKTD
HCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECC
PRHPDPSVRDWVAFRIIDTSRYPHPLVGDRYVAWPTYNFAAAVDDKLMGVTHILRAKEHM
CCCCCCCHHHEEEEEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
QNTIKQQFLYKHLGWSYPHVVHFGRLKLEGFIMSKSTLKRFLDAGISRGIDDPRFATIAG
HHHHHHHHHHHHHCCCCCHHEEECEEEEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHH
LRRRGIVPEAIKKLIMEVGVKYTDASISYDNLAAINRSIIDPRAKRIMAALSPVPVEVEG
HHHCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEECC
LPWREKEFRIPFHPSGQLGERIVKLAGPKATIYVSQSDAVQLAKGNIVRLMEAFNIEVLG
CCCCCCCEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCEEHHCCCHHHHHHHHCEEEEC
ASPRRVRARYHSLTVDEARQHNAPIIQWVPATDNIAVEVLKPEGLDLVQERGLAEPAAAQ
CCHHHHHHHHHHCCHHHHHHCCCCEEEEECCCCCEEEEEECCCCCHHHHHCCCCCCHHHC
LKPGEIIQLVRIGFARVDSVETDEKGKVRKVVLIYAHD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MLNAVGHGGRAAVGPVMGKIMAERPDLRPQAKQIVAIVREVVEEVNKLSLEEQKRLLEEK
CCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YSWVIEKKLQKRREAVEKKLPPLPDAEEGRVVTRFAPNPDFVIHLGNARPALLSYEYAVM
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCEEEEEEEE
YRGKMILRFEDTDPRIKTPLPEAYELIREDLKWLGIRWDEEYIQSLRMHIYYDIARKLIE
ECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
VGGAYVDDRSPEEFRRYRDEGRLEDYPPRKRSVEENLELWDKMVSGAFGEGEAVLRVKTD
HCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECC
PRHPDPSVRDWVAFRIIDTSRYPHPLVGDRYVAWPTYNFAAAVDDKLMGVTHILRAKEHM
CCCCCCCHHHEEEEEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
QNTIKQQFLYKHLGWSYPHVVHFGRLKLEGFIMSKSTLKRFLDAGISRGIDDPRFATIAG
HHHHHHHHHHHHHCCCCCHHEEECEEEEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHH
LRRRGIVPEAIKKLIMEVGVKYTDASISYDNLAAINRSIIDPRAKRIMAALSPVPVEVEG
HHHCCCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEECC
LPWREKEFRIPFHPSGQLGERIVKLAGPKATIYVSQSDAVQLAKGNIVRLMEAFNIEVLG
CCCCCCCEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCEEHHCCCHHHHHHHHCEEEEC
ASPRRVRARYHSLTVDEARQHNAPIIQWVPATDNIAVEVLKPEGLDLVQERGLAEPAAAQ
CCHHHHHHHHHHCCHHHHHHCCCCEEEEECCCCCEEEEEECCCCCHHHHHCCCCCCHHHC
LKPGEIIQLVRIGFARVDSVETDEKGKVRKVVLIYAHD
CCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA