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The map label for this gene is 124027379

Identifier: 124027379

GI number: 124027379

Start: 471220

End: 472347

Strand: Direct

Name: 124027379

Synonym: Hbut_0492

Alternate gene names: NA

Gene position: 471220-472347 (Clockwise)

Preceding gene: 124027377

Following gene: 124027380

Centisome position: 28.26

GC content: 51.15

Gene sequence:

>1128_bases
ATGGAAATAAGCGTTAACAGGCATGGGAAAGGCGATGAAGAGCTACTCCATAAACCCCATGTAAGGGAAGCAATGCTATG
GGAACCCCTAAGGGATAGACCTGGCTATGTACGCTGCAGCCTCTGTGCTCGCCGTTGCATCATAGCTCCGGGCCGCTACG
GCGTCTGCGGGGTAAGGAAGAATATTAACGGGAAGCTTTACACGCTCGTCTACGGCTTGCTAACAGCAATGAATATTGAT
CCTATTGAGAAGAAGCCGCTCTACCACTTCGAGCCCGGCAGTGGAGTACTATCAATAAGCACTGTCGGCTGCAACTTCTT
CTGCCAGTTCTGCCAGAACTGGGAGATAAGCCAGTCAAGGCTAGAGCGGGGCCTCTACGGTCACTACGTGCCGCCAGATG
AGGTTGTGAGGAAAGCCCTAGATTTCGGGGCAGAGGGCATAGCCTACACCTATAACGAGCCAACAATATTCTTCGAGTAC
ATGTACGACGTGGCTAGGCTTGCAAAGAAGCATAACCTCTACAACGTAATGGTTACCAATGGCTACATTACACCCGAGGC
CATAAGGATGATAGGCCCCTACATGGACGGAGCGACCGTCGACTTTAAGGGCAGTGGAAACCCAGAGTTCTACCGCAAGT
TCATGGCTGTACCCGACCCATCCCCAATCTACGACGCATTGCTGGAGATGAAGAAGCAAGGATGGTGGATAGAGATAACA
AACCTGGTAGTCCCCAAGTATGGCGATAGGGAGGAGGATCTTCGGAGGCTTGCGAGATGGATAGTCGAGAATCTCGGGCC
AGACATACCATTCCACCTGCTACGATTCCACCCAGACTACAGACTACTCAACCTACCACCAACACCGGTTGAGACCCTAG
AAAAGTTGGCAAAGATAGCCAAGGAGGAGGGCTTACACTACGTCTACATAGGCAACGTGTGGGGTCATCCCCTAGAGAAC
ACGTATTGCCCCAAGTGCAATTATGCCGTGGTAGAGAGAAGAGGCTTCACCATAATAGCATGGCGTCTCACAAAGGACAA
TCAGTGCCCAAAGTGCGGTCACCGAATAGCGATACGAGGAAAATTCCATGGTGGTGGAGGTGTAATACTACCACTAATGG
TGTACTAG

Upstream 100 bases:

>100_bases
GTTACGCATATCTCAGCAATTGTGCCAAGCTGCATGGATACTGATACTATAATATTAGTGGTTAAGTCTACACAACAATA
TAGGGATGGGTGAGCAGGTT

Downstream 100 bases:

>100_bases
CTCATTGCTAGGCAAGTTGTTGTTCCGGCCTACAACATAGGTGGCACACCTAGATGAAAGCCTCCTGGAGCAACATTAGG
GAATACCATATAGCTCCACT

Product: pyruvate-formate lyase-activating enzyme, PflA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MEISVNRHGKGDEELLHKPHVREAMLWEPLRDRPGYVRCSLCARRCIIAPGRYGVCGVRKNINGKLYTLVYGLLTAMNID
PIEKKPLYHFEPGSGVLSISTVGCNFFCQFCQNWEISQSRLERGLYGHYVPPDEVVRKALDFGAEGIAYTYNEPTIFFEY
MYDVARLAKKHNLYNVMVTNGYITPEAIRMIGPYMDGATVDFKGSGNPEFYRKFMAVPDPSPIYDALLEMKKQGWWIEIT
NLVVPKYGDREEDLRRLARWIVENLGPDIPFHLLRFHPDYRLLNLPPTPVETLEKLAKIAKEEGLHYVYIGNVWGHPLEN
TYCPKCNYAVVERRGFTIIAWRLTKDNQCPKCGHRIAIRGKFHGGGGVILPLMVY

Sequences:

>Translated_375_residues
MEISVNRHGKGDEELLHKPHVREAMLWEPLRDRPGYVRCSLCARRCIIAPGRYGVCGVRKNINGKLYTLVYGLLTAMNID
PIEKKPLYHFEPGSGVLSISTVGCNFFCQFCQNWEISQSRLERGLYGHYVPPDEVVRKALDFGAEGIAYTYNEPTIFFEY
MYDVARLAKKHNLYNVMVTNGYITPEAIRMIGPYMDGATVDFKGSGNPEFYRKFMAVPDPSPIYDALLEMKKQGWWIEIT
NLVVPKYGDREEDLRRLARWIVENLGPDIPFHLLRFHPDYRLLNLPPTPVETLEKLAKIAKEEGLHYVYIGNVWGHPLEN
TYCPKCNYAVVERRGFTIIAWRLTKDNQCPKCGHRIAIRGKFHGGGGVILPLMVY
>Mature_375_residues
MEISVNRHGKGDEELLHKPHVREAMLWEPLRDRPGYVRCSLCARRCIIAPGRYGVCGVRKNINGKLYTLVYGLLTAMNID
PIEKKPLYHFEPGSGVLSISTVGCNFFCQFCQNWEISQSRLERGLYGHYVPPDEVVRKALDFGAEGIAYTYNEPTIFFEY
MYDVARLAKKHNLYNVMVTNGYITPEAIRMIGPYMDGATVDFKGSGNPEFYRKFMAVPDPSPIYDALLEMKKQGWWIEIT
NLVVPKYGDREEDLRRLARWIVENLGPDIPFHLLRFHPDYRLLNLPPTPVETLEKLAKIAKEEGLHYVYIGNVWGHPLEN
TYCPKCNYAVVERRGFTIIAWRLTKDNQCPKCGHRIAIRGKFHGGGGVILPLMVY

Specific function: Unknown

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR016431
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 43067; Mature: 43067

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEISVNRHGKGDEELLHKPHVREAMLWEPLRDRPGYVRCSLCARRCIIAPGRYGVCGVRK
CEEECCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHEECCCCCCEEEEEE
NINGKLYTLVYGLLTAMNIDPIEKKPLYHFEPGSGVLSISTVGCNFFCQFCQNWEISQSR
CCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEEEHHHHHHHHHHCCCCCCHHH
LERGLYGHYVPPDEVVRKALDFGAEGIAYTYNEPTIFFEYMYDVARLAKKHNLYNVMVTN
HHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCEEEHHHHHHHHHHHHHCCEEEEEEEC
GYITPEAIRMIGPYMDGATVDFKGSGNPEFYRKFMAVPDPSPIYDALLEMKKQGWWIEIT
CEECHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEE
NLVVPKYGDREEDLRRLARWIVENLGPDIPFHLLRFHPDYRLLNLPPTPVETLEKLAKIA
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHH
KEEGLHYVYIGNVWGHPLENTYCPKCNYAVVERRGFTIIAWRLTKDNQCPKCGHRIAIRG
HHCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCEEEEEE
KFHGGGGVILPLMVY
EEECCCCEEEEEEEC
>Mature Secondary Structure
MEISVNRHGKGDEELLHKPHVREAMLWEPLRDRPGYVRCSLCARRCIIAPGRYGVCGVRK
CEEECCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHEECCCCCCEEEEEE
NINGKLYTLVYGLLTAMNIDPIEKKPLYHFEPGSGVLSISTVGCNFFCQFCQNWEISQSR
CCCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEEEHHHHHHHHHHCCCCCCHHH
LERGLYGHYVPPDEVVRKALDFGAEGIAYTYNEPTIFFEYMYDVARLAKKHNLYNVMVTN
HHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCEEEHHHHHHHHHHHHHCCEEEEEEEC
GYITPEAIRMIGPYMDGATVDFKGSGNPEFYRKFMAVPDPSPIYDALLEMKKQGWWIEIT
CEECHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEE
NLVVPKYGDREEDLRRLARWIVENLGPDIPFHLLRFHPDYRLLNLPPTPVETLEKLAKIA
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHH
KEEGLHYVYIGNVWGHPLENTYCPKCNYAVVERRGFTIIAWRLTKDNQCPKCGHRIAIRG
HHCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCEEEEEE
KFHGGGGVILPLMVY
EEECCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]