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The map label for this gene is 124027224

Identifier: 124027224

GI number: 124027224

Start: 318884

End: 320041

Strand: Direct

Name: 124027224

Synonym: Hbut_0327

Alternate gene names: NA

Gene position: 318884-320041 (Clockwise)

Preceding gene: 124027216

Following gene: 124027226

Centisome position: 19.13

GC content: 60.36

Gene sequence:

>1158_bases
TTGGGGCGCTGTCTGCTCTGCGGGCGCGAGGGGCTTGTAGCCTCCTCTATCGGCGTCTGCGCCGAGTGTCTGCGCCAGCA
TCCTGGGGAGGCCCTGGAGCTTGTTTGGAGCCGGAGGGCCCGGTGGAGGAGAGAGCTTGGGTTACCGCCCGAACCACCCA
GGGACCCTAGTGGTGTTCCGTGCAGGCTATGCGTTAACGAGTGCAGGATACCACCTGGTGGCCGTGGCTACTGCGGCATC
TGGGCCAACCGCGGCGGAAGGCTCGAGCCGATAACCGGCCGTGGCAGGTTGGCTGTCTACACTTACCTCGACCCCCACCC
AACAAACTGTGTCGCTGAACCCGTCTGCCCAGCCAGCACGTCGAGGGGCTATCCACGGTACACGTTTACCCGGGGCGTTG
AGAAGGGCTACTATAACCTAGCCGTGTTTATGGCTGGGTGCCCGCTCGACTGCATTTTCTGCCAGAACTGGGAGCACAAA
ACGATGATAGCCCACGGCAGGATAGCAGCTAGGTACGTGATGAGTGTGGAGAAGCTTGTAGAGGAGGCTCTCAACCCGAG
GGTGACATGTGTCTGCTACTTCGGAGGGGACCCAACACCATACATGCCCATGCTGATAGAAGCCTCAAGGAGGATCCTTG
AAGAGGCCAGAAAGAAGGGTCAATGGCCTAAAAGGATTTGCTGGGAGACCGACGGCCTGGCAAACCCCGCTGTCCTTCGG
CAAGCCGCGAGGCTGAGCCTGGAGAGTGGCGGCATAGTGAAGATTGACTGGAAGGCCTGGACACCAAGCATATACGAGGC
GCTAACCGGCGTGGACGGCGCCAAGGCTGTTGAGAGGTTGAAGGAGAATACTAGGATGCTAGCCGAGATGGCTGCTGAGA
GGCCGGAACCACCGCTCCTCGTGGTCAGCATACTACTCGTACCGGGCTACGTTGATGCTAGGGAGGTCCGGGGTATAGCG
GGCTACGTGGCAGGCCTCATGGAGGAGTATGGCGTGAACATACCCGTTGTCCTGCTAGCATTCCACCCCGACTACCTTGC
CAGGGACCTGCCGCCAACCAGCCGCAGACACGCCGCCGAGGCGAGAAAAGCCGTGCTCGAGGCAGGCGTGAAAGAGGTCT
ACATCGGGAACATCTGGCTGCTGGGAGACTACTATTAG

Upstream 100 bases:

>100_bases
TAGGGTTTGCTTCTAGGAGCATGAGGGAGGGCCATGAGGGTACTTCACAAGTCTTGCCTGGCTTCCAGGCTACTGGTTCG
TGGCGAGGGCTGACCCGGTC

Downstream 100 bases:

>100_bases
TCGCTGCCGCCCAGGCTATTATCCAGCATGGACGCTATCTCCCGCGCTATCTCGCCCGCCCTCAACGTAGCATCACGAAC
GGCATCGATCACAGCCGACT

Product: pyruvate-formate lyase-activating enzyme, PflA

Products: NA

Alternate protein names: Radical SAM Domain Protein; Pyruvate-Formate Lyase-Activating; Radical Sam Domain Protein; Glycyl Radical-Activating; Pyruvate Formate-Lyase Activating; PflA Pyruvate-Formate Lyase-Activating; Pyruvate Formate-Lyase Activating Related Protein; Pyruvate-Formate Lyase-Activating PflA; Pyruvate Formate-Lyase Activating -Like Protein; Radical SAM Family Protein

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MGRCLLCGREGLVASSIGVCAECLRQHPGEALELVWSRRARWRRELGLPPEPPRDPSGVPCRLCVNECRIPPGGRGYCGI
WANRGGRLEPITGRGRLAVYTYLDPHPTNCVAEPVCPASTSRGYPRYTFTRGVEKGYYNLAVFMAGCPLDCIFCQNWEHK
TMIAHGRIAARYVMSVEKLVEEALNPRVTCVCYFGGDPTPYMPMLIEASRRILEEARKKGQWPKRICWETDGLANPAVLR
QAARLSLESGGIVKIDWKAWTPSIYEALTGVDGAKAVERLKENTRMLAEMAAERPEPPLLVVSILLVPGYVDAREVRGIA
GYVAGLMEEYGVNIPVVLLAFHPDYLARDLPPTSRRHAAEARKAVLEAGVKEVYIGNIWLLGDYY

Sequences:

>Translated_385_residues
MGRCLLCGREGLVASSIGVCAECLRQHPGEALELVWSRRARWRRELGLPPEPPRDPSGVPCRLCVNECRIPPGGRGYCGI
WANRGGRLEPITGRGRLAVYTYLDPHPTNCVAEPVCPASTSRGYPRYTFTRGVEKGYYNLAVFMAGCPLDCIFCQNWEHK
TMIAHGRIAARYVMSVEKLVEEALNPRVTCVCYFGGDPTPYMPMLIEASRRILEEARKKGQWPKRICWETDGLANPAVLR
QAARLSLESGGIVKIDWKAWTPSIYEALTGVDGAKAVERLKENTRMLAEMAAERPEPPLLVVSILLVPGYVDAREVRGIA
GYVAGLMEEYGVNIPVVLLAFHPDYLARDLPPTSRRHAAEARKAVLEAGVKEVYIGNIWLLGDYY
>Mature_384_residues
GRCLLCGREGLVASSIGVCAECLRQHPGEALELVWSRRARWRRELGLPPEPPRDPSGVPCRLCVNECRIPPGGRGYCGIW
ANRGGRLEPITGRGRLAVYTYLDPHPTNCVAEPVCPASTSRGYPRYTFTRGVEKGYYNLAVFMAGCPLDCIFCQNWEHKT
MIAHGRIAARYVMSVEKLVEEALNPRVTCVCYFGGDPTPYMPMLIEASRRILEEARKKGQWPKRICWETDGLANPAVLRQ
AARLSLESGGIVKIDWKAWTPSIYEALTGVDGAKAVERLKENTRMLAEMAAERPEPPLLVVSILLVPGYVDAREVRGIAG
YVAGLMEEYGVNIPVVLLAFHPDYLARDLPPTSRRHAAEARKAVLEAGVKEVYIGNIWLLGDYY

Specific function: Unknown

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 42799; Mature: 42668

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRCLLCGREGLVASSIGVCAECLRQHPGEALELVWSRRARWRRELGLPPEPPRDPSGVP
CCCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
CRLCVNECRIPPGGRGYCGIWANRGGRLEPITGRGRLAVYTYLDPHPTNCVAEPVCPAST
HHEEHHHCCCCCCCCEEEEEEECCCCEECEECCCCCEEEEEEECCCCCCCCCCCCCCCCC
SRGYPRYTFTRGVEKGYYNLAVFMAGCPLDCIFCQNWEHKTMIAHGRIAARYVMSVEKLV
CCCCCCCHHHCCCCCCCEEEEEEEECCCEEEEEECCCCCCEEEECCHHHHHHHHHHHHHH
EEALNPRVTCVCYFGGDPTPYMPMLIEASRRILEEARKKGQWPKRICWETDGLANPAVLR
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHH
QAARLSLESGGIVKIDWKAWTPSIYEALTGVDGAKAVERLKENTRMLAEMAAERPEPPLL
HHHHHEECCCCEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VVSILLVPGYVDAREVRGIAGYVAGLMEEYGVNIPVVLLAFHPDYLARDLPPTSRRHAAE
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCCHHHHHHH
ARKAVLEAGVKEVYIGNIWLLGDYY
HHHHHHHCCCCEEEECCEEEEECCC
>Mature Secondary Structure 
GRCLLCGREGLVASSIGVCAECLRQHPGEALELVWSRRARWRRELGLPPEPPRDPSGVP
CCEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
CRLCVNECRIPPGGRGYCGIWANRGGRLEPITGRGRLAVYTYLDPHPTNCVAEPVCPAST
HHEEHHHCCCCCCCCEEEEEEECCCCEECEECCCCCEEEEEEECCCCCCCCCCCCCCCCC
SRGYPRYTFTRGVEKGYYNLAVFMAGCPLDCIFCQNWEHKTMIAHGRIAARYVMSVEKLV
CCCCCCCHHHCCCCCCCEEEEEEEECCCEEEEEECCCCCCEEEECCHHHHHHHHHHHHHH
EEALNPRVTCVCYFGGDPTPYMPMLIEASRRILEEARKKGQWPKRICWETDGLANPAVLR
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHH
QAARLSLESGGIVKIDWKAWTPSIYEALTGVDGAKAVERLKENTRMLAEMAAERPEPPLL
HHHHHEECCCCEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VVSILLVPGYVDAREVRGIAGYVAGLMEEYGVNIPVVLLAFHPDYLARDLPPTSRRHAAE
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCCHHHHHHH
ARKAVLEAGVKEVYIGNIWLLGDYY
HHHHHHHCCCCEEEECCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA