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The map label for this gene is nuoI [H]

Identifier: 124027084

GI number: 124027084

Start: 168325

End: 168870

Strand: Direct

Name: nuoI [H]

Synonym: Hbut_0187

Alternate gene names: 124027084

Gene position: 168325-168870 (Clockwise)

Preceding gene: 124027083

Following gene: 124027085

Centisome position: 10.1

GC content: 57.33

Gene sequence:

>546_bases
ATGAGCCAGAGCGGGCAACCCGGAGCCGGGCAGACGGCAAGCAGTGGTGTTAGGGTCCGCAGGGTTAGTAGGCCAAAGGC
CGGCATAGTGTCGGGCCACGTCTCGGCGATCTCTGCGGCCCTGCGTAGAGCGTCGAAGAAACCCATGACGCTAATGTACC
CCACGGTGGAGGAGGAGAAGCCACAGCTCTTCAGGGGCTTCATACTCTACGACTACGACAAGTGCATAGGCTGCAGCTTG
TGCGCCCAGATATGCCCAGCCAGGGCTATCAAGATGTATCGTGTCCCGGGCGACAAGAGGCTAAGACCCGGCTACGACGT
TGGGCGCTGCATATACTGTGGCCTCTGCACAGACATATGCCCAACAGATGCCCTCATCCTTAGCGACCGGTTCGACCACG
TATTCGAGAAGCTTGAGGACATGATTTTCGATCCGATAGATTGGGCTCAGATCTCCAAGAAGATTAGGGAGGAGAAGCCC
ACGAGGAAGAGGGTTAGAACACTCGTAGATGAGGAGGTGGGTCTCCGGTATGAGCCTATCGGCTAG

Upstream 100 bases:

>100_bases
CCGCTTCATGCCCCTAGCCTTTGCCGCCCTCATACTTGCAGTATTCGTGCGCTCGCTCTATCTCGGCTAAGTATGGGAAG
TAGCGGAGGTGTAGGCTTGG

Downstream 100 bases:

>100_bases
CCTTGAGAGTGTTCTCTACTACTCTGTACTGCTCGGCTCGCTAGCTACAGTGGCAGTGGGCTCCATAGCAGCAGTAAGGG
CTAGGAAGACCATCTACAGC

Product: NADH-ubiquinone oxidoreductase subunit 8

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MSQSGQPGAGQTASSGVRVRRVSRPKAGIVSGHVSAISAALRRASKKPMTLMYPTVEEEKPQLFRGFILYDYDKCIGCSL
CAQICPARAIKMYRVPGDKRLRPGYDVGRCIYCGLCTDICPTDALILSDRFDHVFEKLEDMIFDPIDWAQISKKIREEKP
TRKRVRTLVDEEVGLRYEPIG

Sequences:

>Translated_181_residues
MSQSGQPGAGQTASSGVRVRRVSRPKAGIVSGHVSAISAALRRASKKPMTLMYPTVEEEKPQLFRGFILYDYDKCIGCSL
CAQICPARAIKMYRVPGDKRLRPGYDVGRCIYCGLCTDICPTDALILSDRFDHVFEKLEDMIFDPIDWAQISKKIREEKP
TRKRVRTLVDEEVGLRYEPIG
>Mature_180_residues
SQSGQPGAGQTASSGVRVRRVSRPKAGIVSGHVSAISAALRRASKKPMTLMYPTVEEEKPQLFRGFILYDYDKCIGCSLC
AQICPARAIKMYRVPGDKRLRPGYDVGRCIYCGLCTDICPTDALILSDRFDHVFEKLEDMIFDPIDWAQISKKIREEKPT
RKRVRTLVDEEVGLRYEPIG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=108, Percent_Identity=35.1851851851852, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1788617, Length=124, Percent_Identity=32.258064516129, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789075, Length=79, Percent_Identity=34.1772151898734, Blast_Score=61, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17555194, Length=126, Percent_Identity=34.1269841269841, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17864306, Length=117, Percent_Identity=34.1880341880342, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 20330; Mature: 20199

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSGQPGAGQTASSGVRVRRVSRPKAGIVSGHVSAISAALRRASKKPMTLMYPTVEEEK
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
PQLFRGFILYDYDKCIGCSLCAQICPARAIKMYRVPGDKRLRPGYDVGRCIYCGLCTDIC
HHHHHHHHEECCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHCCC
PTDALILSDRFDHVFEKLEDMIFDPIDWAQISKKIREEKPTRKRVRTLVDEEVGLRYEPI
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCEECCC
G
C
>Mature Secondary Structure 
SQSGQPGAGQTASSGVRVRRVSRPKAGIVSGHVSAISAALRRASKKPMTLMYPTVEEEK
CCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
PQLFRGFILYDYDKCIGCSLCAQICPARAIKMYRVPGDKRLRPGYDVGRCIYCGLCTDIC
HHHHHHHHEECCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHCCC
PTDALILSDRFDHVFEKLEDMIFDPIDWAQISKKIREEKPTRKRVRTLVDEEVGLRYEPI
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCEECCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA