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The map label for this gene is dphB [H]

Identifier: 124027028

GI number: 124027028

Start: 112370

End: 113164

Strand: Reverse

Name: dphB [H]

Synonym: Hbut_0128

Alternate gene names: 124027028

Gene position: 113164-112370 (Counterclockwise)

Preceding gene: 124027030

Following gene: 124027025

Centisome position: 6.79

GC content: 56.1

Gene sequence:

>795_bases
TTGCCGCTTTACCTGGTTGGTGCCGGTATTTCCGCTAGGTATCTTACGCTTAGAGCGATACAGCTCATAGGCTCCGCTGA
TGTGGTTTACATTGATACATACACCAGTATAGCGCCGGGCATCGATGAGGAGCTGGTAACTAGGCTTAACCCGCGGGCCC
GCATTGTTAAGGCCTCGAGGGAACTGCTTGAGCAAGGGTCGAGGAGGATTGTCGAGGAGGCCCAGAGAATGAATGTTGTC
GTGCTTGTACCTGGTGATCCCTTACACGCTACGACCCATATAGCGCTGTTGCTCGAAGCTCGTCGGGCAGGAGTTGACGC
CGAGGTTGTGCCAGGGGTATCCGGGCTCCAGGCTGTGGTTGATGCTACGGGGTTGCAGGTCTACAGATTTGGGAGGCCGG
TGACTCTCGTCTACCCCGAGGAGGGCTTTAAGCCATATAGCACGATCGATGTTGTAAGGAATAACCGTAGAGCAGGTTTG
CACACAATGGTCCTCCTTGACCTCCGCCTAGACGAAGGTCGTGCAATGACTGTTCCAGAGGCTGTCGAGCTGCTTCTGAG
CCTAGAGGAGGAACTAGCCCATGAGGAAGGCTGGGAGCCCGAGCTGAGAGACGCGGTAATTGTTGGTGTGGCTAGGGCAG
GGTTGGAGGATGGAGTGTGTATAGCGGGGCGCCCCGAGGAGGTTGCCTCTGCTAGTTATCCGCCGCCGCCTCACACGCTT
GTAGTAACGGCGCCACGTCTACATCCTATGGAGGCTGAGGCGTTGAAAACATTGTGTGGTTGCAAGAGCTGCTAG

Upstream 100 bases:

>100_bases
CAACGCGTGGCCTGGAGGGCACACTAGTCACCGGGCGTAGGGGCTGGCTGTGTAGGACTATAATCTCCAGCGGCACGACT
CTCAAGGCGGAGGGAGGGTT

Downstream 100 bases:

>100_bases
GCCTACATGTGGGTGGGGGGCTGCGGCTCCTCAACCTCTATGTACTCGCAGGTCGAGGGTATGAGGAGGAGTAAGGGACC
TGCATCACTCGCCTCCACAT

Product: diphthine synthase

Products: NA

Alternate protein names: Diphthamide biosynthesis methyltransferase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MPLYLVGAGISARYLTLRAIQLIGSADVVYIDTYTSIAPGIDEELVTRLNPRARIVKASRELLEQGSRRIVEEAQRMNVV
VLVPGDPLHATTHIALLLEARRAGVDAEVVPGVSGLQAVVDATGLQVYRFGRPVTLVYPEEGFKPYSTIDVVRNNRRAGL
HTMVLLDLRLDEGRAMTVPEAVELLLSLEEELAHEEGWEPELRDAVIVGVARAGLEDGVCIAGRPEEVASASYPPPPHTL
VVTAPRLHPMEAEALKTLCGCKSC

Sequences:

>Translated_264_residues
MPLYLVGAGISARYLTLRAIQLIGSADVVYIDTYTSIAPGIDEELVTRLNPRARIVKASRELLEQGSRRIVEEAQRMNVV
VLVPGDPLHATTHIALLLEARRAGVDAEVVPGVSGLQAVVDATGLQVYRFGRPVTLVYPEEGFKPYSTIDVVRNNRRAGL
HTMVLLDLRLDEGRAMTVPEAVELLLSLEEELAHEEGWEPELRDAVIVGVARAGLEDGVCIAGRPEEVASASYPPPPHTL
VVTAPRLHPMEAEALKTLCGCKSC
>Mature_263_residues
PLYLVGAGISARYLTLRAIQLIGSADVVYIDTYTSIAPGIDEELVTRLNPRARIVKASRELLEQGSRRIVEEAQRMNVVV
LVPGDPLHATTHIALLLEARRAGVDAEVVPGVSGLQAVVDATGLQVYRFGRPVTLVYPEEGFKPYSTIDVVRNNRRAGLH
TMVLLDLRLDEGRAMTVPEAVELLLSLEEELAHEEGWEPELRDAVIVGVARAGLEDGVCIAGRPEEVASASYPPPPHTLV
VTAPRLHPMEAEALKTLCGCKSC

Specific function: Required for the methylation step in diphthamide biosynthesis [H]

COG id: COG1798

COG function: function code J; Diphthamide biosynthesis methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diphthine synthase family [H]

Homologues:

Organism=Homo sapiens, GI117190324, Length=269, Percent_Identity=32.7137546468402, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI117190326, Length=270, Percent_Identity=32.5925925925926, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI117190262, Length=270, Percent_Identity=32.5925925925926, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17531369, Length=282, Percent_Identity=29.4326241134752, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323201, Length=262, Percent_Identity=33.206106870229, Blast_Score=139, Evalue=4e-34,
Organism=Drosophila melanogaster, GI28572120, Length=270, Percent_Identity=32.5925925925926, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR004551 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.98 [H]

Molecular weight: Translated: 28653; Mature: 28522

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLYLVGAGISARYLTLRAIQLIGSADVVYIDTYTSIAPGIDEELVTRLNPRARIVKASR
CCEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHH
ELLEQGSRRIVEEAQRMNVVVLVPGDPLHATTHIALLLEARRAGVDAEVVPGVSGLQAVV
HHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHH
DATGLQVYRFGRPVTLVYPEEGFKPYSTIDVVRNNRRAGLHTMVLLDLRLDEGRAMTVPE
HHCCCEEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEHHH
AVELLLSLEEELAHEEGWEPELRDAVIVGVARAGLEDGVCIAGRPEEVASASYPPPPHTL
HHHHHHHHHHHHHHHCCCCCCHHHHEEEHHHHCCCCCCEEECCCCHHHHCCCCCCCCCEE
VVTAPRLHPMEAEALKTLCGCKSC
EEECCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
PLYLVGAGISARYLTLRAIQLIGSADVVYIDTYTSIAPGIDEELVTRLNPRARIVKASR
CEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHH
ELLEQGSRRIVEEAQRMNVVVLVPGDPLHATTHIALLLEARRAGVDAEVVPGVSGLQAVV
HHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHHH
DATGLQVYRFGRPVTLVYPEEGFKPYSTIDVVRNNRRAGLHTMVLLDLRLDEGRAMTVPE
HHCCCEEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEHHH
AVELLLSLEEELAHEEGWEPELRDAVIVGVARAGLEDGVCIAGRPEEVASASYPPPPHTL
HHHHHHHHHHHHHHHCCCCCCHHHHEEEHHHHCCCCCCEEECCCCHHHHCCCCCCCCCEE
VVTAPRLHPMEAEALKTLCGCKSC
EEECCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA