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The map label for this gene is pyrG [H]

Identifier: 124027014

GI number: 124027014

Start: 101852

End: 103459

Strand: Direct

Name: pyrG [H]

Synonym: Hbut_0114

Alternate gene names: 124027014

Gene position: 101852-103459 (Clockwise)

Preceding gene: 124027013

Following gene: 124027015

Centisome position: 6.11

GC content: 53.17

Gene sequence:

>1608_bases
ATGACAAAATACATCTTTGTCACGGGCGGAGTCCTCAGCAGCGTTGGCAAAGGTATAACTACAGCCTCTATAGGCCTCCT
CCTCAAGGCGCGCGGCTACAACGTTACAGCGATAAAGATAGATCCATACATCAACGTTGACGCAGGCACTATGAACCCCT
ACATGCACGGCGAGGTCTTCGTAACCGAGGACGGTGGCGAGACAGACCTAGACCTAGGCCACTATGAGAGGTTCCTCGAC
ACCTTCCTCTCCAAGAAGAACAACATCACGACAGGACAGATCTACCTATCAGTGATAGAGAGGGAGCGACGTGGCGACTA
CCTGGGACAAACAGTACAAGTAATCCCCCACATAACAAACGAGATTAAGGACAGGATAAGGGATGTTGCAAGAGAAACCT
CAGCCGACATAGTGCTGGTCGAGATTGGTGGCACTGTAGGTGATATTGAGGGGCTACCATTCCTAGAGGCCGTGAGGCAG
ATGAGGATGGAGGAAGGCTACAATAATACACTATTCATACACGTGGCACTGGTCCCCGTTCTCTCGACGACGGGTGAGCA
GAAGACCAAGCCTGTACAGCATAGTGTACAAGAGCTACGCCGCATAGGCATACAGCCAGACGCCATAATAGCCCGTAGTA
CACGCCCACTAGAGGAGGAGGCCCGAAACAAGATAGCTCTCTACGCAAACCTACCCCCAAGCGCAGTGTTCAGTAACCCA
GACGTCGAGGTGATCTATGAGGTTCCACTAATCCTCGAGGAGCAGGGCCTCGGAAACTTTATCGTTGAGAGGCTCGGCCT
TGAGAAGCGTAAGCCAGACCTAGACGCTTGGAGGGAGTTTGTTCGCCGCGTTAAGGAGGCCTCAAAACCGGTAAGGATAG
CAATGGTTGGCAAGTACACCAAGCTCAAGGACAGCTACCTAAGCATCATAGAGGCGCTACGTCACGCCGGAGCAGCCCTC
GGCGTTAAGCCGGTACTAAACTGGTACGAGTCAACACTCATTGAGAGGGGTAAACTGAACCCCCGCCGGCCAGTAGAGGA
GAACGACGCAGTCATAGTGCTGCCGGGCTTTGGCGCAAGAGGAGCTGAGGGCAAGATAGCGGTCATACGCGAGGTCATAG
AAGCTGGGAAACCATTCCTAGGCATATGCTTTGGCATGCAGCTAGCAGTCATCGCTATAGCTCGGCACCTGGCAGGCCTC
GAGGACGCTAATAGCACAGAGATAGATCCAGCAACACCTTACCCCGTTGTAGACCTGCTTGAGAGTCAGCGTTACCTCAC
CAAGCTAGGAGGAACAATGAGGTTAGGCTCCTACCCGATAAAGCTGATAAGAGGCACGCTAGTCTACAACCTCTACGAGG
GTAAGGAGATAGTATATGAGAGACATCGCCACCGCTACGAGGTAAACCCCAAGTACCTAGACAAGCTAGTAGCGTCGGGC
ATGGAGGTTTCAGGCTACAGCCTGGAAGGTGGAAGGGTCGAGTTCATAGAGCTGAGAAGCCACCGATTCTTCGTTGGGAG
TCAGCCGCACCCAGAGTTCCGGAGCAGACCAATGAAGCCAGCACCGCTATTCTTAGGCCTACTAAGGGCAGCAACTAGCC
AGAACTAG

Upstream 100 bases:

>100_bases
AGATACCCTGACATAATCGCGAAGATAAAACCGCACTCGCCGCAAGTAGACGAACTCCTTAAAGCTTGGCAGTAGCTGTA
GCCACCCCACCGGGCAGCAC

Downstream 100 bases:

>100_bases
GCAAACCCTAGCACCTTTTAAACCACGTAGAATCCTCTATACACAACAATACAGGCCCGCGAATCCCGTCGCGGAAACTA
CTCGCGAGTGGTGACTCAGT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MTKYIFVTGGVLSSVGKGITTASIGLLLKARGYNVTAIKIDPYINVDAGTMNPYMHGEVFVTEDGGETDLDLGHYERFLD
TFLSKKNNITTGQIYLSVIERERRGDYLGQTVQVIPHITNEIKDRIRDVARETSADIVLVEIGGTVGDIEGLPFLEAVRQ
MRMEEGYNNTLFIHVALVPVLSTTGEQKTKPVQHSVQELRRIGIQPDAIIARSTRPLEEEARNKIALYANLPPSAVFSNP
DVEVIYEVPLILEEQGLGNFIVERLGLEKRKPDLDAWREFVRRVKEASKPVRIAMVGKYTKLKDSYLSIIEALRHAGAAL
GVKPVLNWYESTLIERGKLNPRRPVEENDAVIVLPGFGARGAEGKIAVIREVIEAGKPFLGICFGMQLAVIAIARHLAGL
EDANSTEIDPATPYPVVDLLESQRYLTKLGGTMRLGSYPIKLIRGTLVYNLYEGKEIVYERHRHRYEVNPKYLDKLVASG
MEVSGYSLEGGRVEFIELRSHRFFVGSQPHPEFRSRPMKPAPLFLGLLRAATSQN

Sequences:

>Translated_535_residues
MTKYIFVTGGVLSSVGKGITTASIGLLLKARGYNVTAIKIDPYINVDAGTMNPYMHGEVFVTEDGGETDLDLGHYERFLD
TFLSKKNNITTGQIYLSVIERERRGDYLGQTVQVIPHITNEIKDRIRDVARETSADIVLVEIGGTVGDIEGLPFLEAVRQ
MRMEEGYNNTLFIHVALVPVLSTTGEQKTKPVQHSVQELRRIGIQPDAIIARSTRPLEEEARNKIALYANLPPSAVFSNP
DVEVIYEVPLILEEQGLGNFIVERLGLEKRKPDLDAWREFVRRVKEASKPVRIAMVGKYTKLKDSYLSIIEALRHAGAAL
GVKPVLNWYESTLIERGKLNPRRPVEENDAVIVLPGFGARGAEGKIAVIREVIEAGKPFLGICFGMQLAVIAIARHLAGL
EDANSTEIDPATPYPVVDLLESQRYLTKLGGTMRLGSYPIKLIRGTLVYNLYEGKEIVYERHRHRYEVNPKYLDKLVASG
MEVSGYSLEGGRVEFIELRSHRFFVGSQPHPEFRSRPMKPAPLFLGLLRAATSQN
>Mature_534_residues
TKYIFVTGGVLSSVGKGITTASIGLLLKARGYNVTAIKIDPYINVDAGTMNPYMHGEVFVTEDGGETDLDLGHYERFLDT
FLSKKNNITTGQIYLSVIERERRGDYLGQTVQVIPHITNEIKDRIRDVARETSADIVLVEIGGTVGDIEGLPFLEAVRQM
RMEEGYNNTLFIHVALVPVLSTTGEQKTKPVQHSVQELRRIGIQPDAIIARSTRPLEEEARNKIALYANLPPSAVFSNPD
VEVIYEVPLILEEQGLGNFIVERLGLEKRKPDLDAWREFVRRVKEASKPVRIAMVGKYTKLKDSYLSIIEALRHAGAALG
VKPVLNWYESTLIERGKLNPRRPVEENDAVIVLPGFGARGAEGKIAVIREVIEAGKPFLGICFGMQLAVIAIARHLAGLE
DANSTEIDPATPYPVVDLLESQRYLTKLGGTMRLGSYPIKLIRGTLVYNLYEGKEIVYERHRHRYEVNPKYLDKLVASGM
EVSGYSLEGGRVEFIELRSHRFFVGSQPHPEFRSRPMKPAPLFLGLLRAATSQN

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=549, Percent_Identity=47.9052823315118, Blast_Score=497, Evalue=1e-141,
Organism=Homo sapiens, GI28559085, Length=550, Percent_Identity=47.6363636363636, Blast_Score=490, Evalue=1e-138,
Organism=Homo sapiens, GI28559083, Length=550, Percent_Identity=47.6363636363636, Blast_Score=490, Evalue=1e-138,
Organism=Homo sapiens, GI221316689, Length=550, Percent_Identity=47.6363636363636, Blast_Score=490, Evalue=1e-138,
Organism=Escherichia coli, GI1789142, Length=541, Percent_Identity=51.2014787430684, Blast_Score=509, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI25148299, Length=603, Percent_Identity=40.4643449419569, Blast_Score=419, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319432, Length=559, Percent_Identity=42.5760286225402, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6322563, Length=562, Percent_Identity=42.3487544483986, Blast_Score=439, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24664469, Length=551, Percent_Identity=47.9128856624319, Blast_Score=500, Evalue=1e-142,
Organism=Drosophila melanogaster, GI21357815, Length=496, Percent_Identity=45.7661290322581, Blast_Score=422, Evalue=1e-118,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59680; Mature: 59548

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYIFVTGGVLSSVGKGITTASIGLLLKARGYNVTAIKIDPYINVDAGTMNPYMHGEVF
CCEEEEEECHHHHHHCCCCHHHHHEEEEEECCCEEEEEEECCEEEECCCCCCCCCCCEEE
VTEDGGETDLDLGHYERFLDTFLSKKNNITTGQIYLSVIERERRGDYLGQTVQVIPHITN
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EIKDRIRDVARETSADIVLVEIGGTVGDIEGLPFLEAVRQMRMEEGYNNTLFIHVALVPV
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEHHH
LSTTGEQKTKPVQHSVQELRRIGIQPDAIIARSTRPLEEEARNKIALYANLPPSAVFSNP
HHCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCCEEEEEECCCCHHHCCCC
DVEVIYEVPLILEEQGLGNFIVERLGLEKRKPDLDAWREFVRRVKEASKPVRIAMVGKYT
CEEEEEECCEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCH
KLKDSYLSIIEALRHAGAALGVKPVLNWYESTLIERGKLNPRRPVEENDAVIVLPGFGAR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCC
GAEGKIAVIREVIEAGKPFLGICFGMQLAVIAIARHLAGLEDANSTEIDPATPYPVVDLL
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
ESQRYLTKLGGTMRLGSYPIKLIRGTLVYNLYEGKEIVYERHRHRYEVNPKYLDKLVASG
HHHHHHHHCCCCEEECCCCHHHHHHHHEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHCC
MEVSGYSLEGGRVEFIELRSHRFFVGSQPHPEFRSRPMKPAPLFLGLLRAATSQN
CCCCCEECCCCCEEEEEEECCEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKYIFVTGGVLSSVGKGITTASIGLLLKARGYNVTAIKIDPYINVDAGTMNPYMHGEVF
CEEEEEECHHHHHHCCCCHHHHHEEEEEECCCEEEEEEECCEEEECCCCCCCCCCCEEE
VTEDGGETDLDLGHYERFLDTFLSKKNNITTGQIYLSVIERERRGDYLGQTVQVIPHITN
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EIKDRIRDVARETSADIVLVEIGGTVGDIEGLPFLEAVRQMRMEEGYNNTLFIHVALVPV
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEHHH
LSTTGEQKTKPVQHSVQELRRIGIQPDAIIARSTRPLEEEARNKIALYANLPPSAVFSNP
HHCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCHHHHCCCEEEEEECCCCHHHCCCC
DVEVIYEVPLILEEQGLGNFIVERLGLEKRKPDLDAWREFVRRVKEASKPVRIAMVGKYT
CEEEEEECCEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCH
KLKDSYLSIIEALRHAGAALGVKPVLNWYESTLIERGKLNPRRPVEENDAVIVLPGFGAR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCC
GAEGKIAVIREVIEAGKPFLGICFGMQLAVIAIARHLAGLEDANSTEIDPATPYPVVDLL
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
ESQRYLTKLGGTMRLGSYPIKLIRGTLVYNLYEGKEIVYERHRHRYEVNPKYLDKLVASG
HHHHHHHHCCCCEEECCCCHHHHHHHHEEEEECCHHHHHHHHHCCCCCCHHHHHHHHHCC
MEVSGYSLEGGRVEFIELRSHRFFVGSQPHPEFRSRPMKPAPLFLGLLRAATSQN
CCCCCEECCCCCEEEEEEECCEEEECCCCCCHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]