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The map label for this gene is tfb [H]

Identifier: 124026957

GI number: 124026957

Start: 43820

End: 44776

Strand: Direct

Name: tfb [H]

Synonym: Hbut_0054

Alternate gene names: 124026957

Gene position: 43820-44776 (Clockwise)

Preceding gene: 124026956

Following gene: 124026958

Centisome position: 2.63

GC content: 53.92

Gene sequence:

>957_bases
GTGTTTGAGGAAGGTTATACCCCCGTAGAGGGGGGAGAGGAGAGCGAGGCAAAAGAAGGTATCGGGGATCGGTGCCCCCC
AGAATATATTGTATTCGATGAGGAGAGAGGCGAGTATATCTGCACGCTTACTGGCGAAGTTGTAGAAGATACTGTTATCG
ATACTGGGCCGGAATGGCGTGCCTATACCCCAGAGGAGAAGACGAAACGTAGTCGTGTCGGTAGCCCACTGACACACACG
CTTCCAGACTATGGTGTTCTCACAACTATCTCTGGCTACCGTGACGCCACTGGACGGAAGCTCGAGGCTAGGCTGCGTAT
AGAGGCTAGTAGGCTGCGCAGACTACAAGCCAAGCTGCGTGCAACAACTAGCATTGAGAAGAACATTGAGCAGGCTGCTA
GGGAGATTACTCGCCTCATTGAGGCGCTCAACCTGCCCCGTAGCGTGATAGACACTGCCATGATGATCTATAGACAAGCT
GCAGAGAAGGGTCTTGTGCGTGGCAGAAGCCTAGAATCCATGGCTGCGGCAGCTGTCTATGCAGCTTGCAGGATTAGGGG
TATACCTAGGAGCATTGACGACATAGCAGAGCTTGTGAAGGGCGGTAGGAAGGAGGTTGCTAGGTGCTACAGGCTCATAG
TGAGAGAGTTAAGGCTCCGCATGCCGATAGTGGATCCGGTACGCTACGTATCAAGGATAACTTCAGCTCTTCGGCTGAGC
CCGGCGGTTGAAAAGAGGGCTGCTGAGATCCTAATCAGGGCTCGCAAGATGGGGCTTACGGCAGGCAAGGATCCAGCTGG
CCTTGCAGCAGCGGCGATATACATCGCCGCTCTTGAACTCGGCGAGAGGAGGACACAGAAGGAGATAGCTGCAGCAGCGG
GTGTAACCGAGGTAACTGTTAGGAACCGCTACAAGGAGCTAGTCCAGAAGCTCAATATAACCCTTCCAGCACAGTAG

Upstream 100 bases:

>100_bases
ACTAAAAGCTTATAAACCCCTACTAAGGAATAGGATGTGTTGGGCTTTAAAGGGTTTCGGGCCATACCCCCCGGGGTACT
ATGCTGTTGGGTGGGGCTAC

Downstream 100 bases:

>100_bases
CTTGGACACTACTCAAGCCTCCGGTAGCTAGGTTTTTCCCTCCTCCAGGCCCCCAGGCCCTGTTACCGGGTGTGTTCTAG
CCCTTGGCCGTGGAGTTTGT

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MFEEGYTPVEGGEESEAKEGIGDRCPPEYIVFDEERGEYICTLTGEVVEDTVIDTGPEWRAYTPEEKTKRSRVGSPLTHT
LPDYGVLTTISGYRDATGRKLEARLRIEASRLRRLQAKLRATTSIEKNIEQAAREITRLIEALNLPRSVIDTAMMIYRQA
AEKGLVRGRSLESMAAAAVYAACRIRGIPRSIDDIAELVKGGRKEVARCYRLIVRELRLRMPIVDPVRYVSRITSALRLS
PAVEKRAAEILIRARKMGLTAGKDPAGLAAAAIYIAALELGERRTQKEIAAAAGVTEVTVRNRYKELVQKLNITLPAQ

Sequences:

>Translated_318_residues
MFEEGYTPVEGGEESEAKEGIGDRCPPEYIVFDEERGEYICTLTGEVVEDTVIDTGPEWRAYTPEEKTKRSRVGSPLTHT
LPDYGVLTTISGYRDATGRKLEARLRIEASRLRRLQAKLRATTSIEKNIEQAAREITRLIEALNLPRSVIDTAMMIYRQA
AEKGLVRGRSLESMAAAAVYAACRIRGIPRSIDDIAELVKGGRKEVARCYRLIVRELRLRMPIVDPVRYVSRITSALRLS
PAVEKRAAEILIRARKMGLTAGKDPAGLAAAAIYIAALELGERRTQKEIAAAAGVTEVTVRNRYKELVQKLNITLPAQ
>Mature_318_residues
MFEEGYTPVEGGEESEAKEGIGDRCPPEYIVFDEERGEYICTLTGEVVEDTVIDTGPEWRAYTPEEKTKRSRVGSPLTHT
LPDYGVLTTISGYRDATGRKLEARLRIEASRLRRLQAKLRATTSIEKNIEQAAREITRLIEALNLPRSVIDTAMMIYRQA
AEKGLVRGRSLESMAAAAVYAACRIRGIPRSIDDIAELVKGGRKEVARCYRLIVRELRLRMPIVDPVRYVSRITSALRLS
PAVEKRAAEILIRARKMGLTAGKDPAGLAAAAIYIAALELGERRTQKEIAAAAGVTEVTVRNRYKELVQKLNITLPAQ

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB) [H]

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TFIIB family [H]

Homologues:

Organism=Homo sapiens, GI4504193, Length=292, Percent_Identity=33.5616438356164, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI22035556, Length=286, Percent_Identity=26.5734265734266, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17564892, Length=287, Percent_Identity=32.404181184669, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17531397, Length=289, Percent_Identity=22.1453287197232, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325343, Length=304, Percent_Identity=26.6447368421053, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321685, Length=191, Percent_Identity=27.2251308900524, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17136758, Length=281, Percent_Identity=33.0960854092527, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI281361961, Length=289, Percent_Identity=26.2975778546713, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24647658, Length=289, Percent_Identity=26.2975778546713, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137 [H]

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon [H]

EC number: NA

Molecular weight: Translated: 35355; Mature: 35355

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00782 TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEEGYTPVEGGEESEAKEGIGDRCPPEYIVFDEERGEYICTLTGEVVEDTVIDTGPEWR
CCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCEEEEECHHHHHHHHHCCCCCCC
AYTPEEKTKRSRVGSPLTHTLPDYGVLTTISGYRDATGRKLEARLRIEASRLRRLQAKLR
CCCCHHHHHHHHCCCCHHHCCCCCCHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ATTSIEKNIEQAAREITRLIEALNLPRSVIDTAMMIYRQAAEKGLVRGRSLESMAAAAVY
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AACRIRGIPRSIDDIAELVKGGRKEVARCYRLIVRELRLRMPIVDPVRYVSRITSALRLS
HHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
PAVEKRAAEILIRARKMGLTAGKDPAGLAAAAIYIAALELGERRTQKEIAAAAGVTEVTV
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RNRYKELVQKLNITLPAQ
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFEEGYTPVEGGEESEAKEGIGDRCPPEYIVFDEERGEYICTLTGEVVEDTVIDTGPEWR
CCCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCEEEEECHHHHHHHHHCCCCCCC
AYTPEEKTKRSRVGSPLTHTLPDYGVLTTISGYRDATGRKLEARLRIEASRLRRLQAKLR
CCCCHHHHHHHHCCCCHHHCCCCCCHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ATTSIEKNIEQAAREITRLIEALNLPRSVIDTAMMIYRQAAEKGLVRGRSLESMAAAAVY
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AACRIRGIPRSIDDIAELVKGGRKEVARCYRLIVRELRLRMPIVDPVRYVSRITSALRLS
HHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
PAVEKRAAEILIRARKMGLTAGKDPAGLAAAAIYIAALELGERRTQKEIAAAAGVTEVTV
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
RNRYKELVQKLNITLPAQ
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA