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The map label for this gene is glyS [H]

Identifier: 124026953

GI number: 124026953

Start: 40063

End: 41844

Strand: Reverse

Name: glyS [H]

Synonym: Hbut_0050

Alternate gene names: 124026953

Gene position: 41844-40063 (Counterclockwise)

Preceding gene: 124026955

Following gene: 124026952

Centisome position: 2.51

GC content: 50.56

Gene sequence:

>1782_bases
ATGGCTAAGCCTGTTGACAAGTATGAGAAGCTTGTAGACCTAGCTATTCGACGAGGGTTCTTCTGGCCAAGCTACGAGAT
ATACGGTGGTGTAGCTGGCTTCTACGATCTCGGCCCTCTCGGTGTGAGGCTTAAGGAGAAGATTTTACAGCTCTGGCGCG
ACTTCTTCATCCGCCGCCACGCAGGCTTCGTGGTAGAGATCGAGACGCCCATAATAGCTCCGTCAAGAGTTTTCGAGGCT
AGCGGACACCTTGAGCACTTCACTGATCCCATCGTGGAGTGCCGGCGTTGCGGCAGGAAGTTTAGGGCTGATCATCTCAT
AGAAGAGAAGCTTGGCATTAAGGCTGAGGGACTGAGCATAGAGGAGATGACACGGCTCATAAGAGAGCATGATATCCGCT
GCCCAGTCTGCGGTGGCGAACTAGGCGAGGTCAAGAAATTTAACCTACTATTCAAGACCACTATTGGCCCCTATAGTGAG
AATGTTGGCTACCTTAGGCCTGAGAATGCACAAGGTATGTTTGTCGCGTTTAAGAGAGTCTACGAGGCTATGAGGCAGAG
ACTTCCCCTGGGCATAGCGCAAATAGGACGAGTAGCTAGGAACGAGATATCACCAAGACAAGGAATGATAAGGCTTAGAG
AGTTCACGATAGCCGAGATGGAGTTCTTCTTTGATCCCGAGGATCCAGGACACAACGGCCTTCTCGACGAACTTCTCGGA
AGGGTTGGAGATACAAAGCTTCGTATCTTACGCGCAGAGGAAAAGGAGAAGGGTGCTGATGAGCCTTCCGAGTACAAGGT
CTCAGAGCTTGTAGAGGAGCGCATCGTTGCTAATCCCTGGCTGGCATACTGGATGGCTGTTGCCAGAGACTTTATCAAAG
CACTAGGCGTGCCGGAGCAGAACATGTACTTCGAGGAAAAGCCGCCAGAAGAGAGGGCACACTACTCTGCCCAGACCTTC
GACCAGCTCGTAAGGGTTAGCAGGTTTGGCTGGATAGAGGTCTCCGGACACTCCTACCGAACCGACTACGACCTCTCACG
CCACATGAAGCATAGCGGCCAGGACCTAAGAGTCTTCAAACAGTACAGGGAGCCGGTAATCGTTAAACGCAAAATGATAT
TCGTTGACAGGGCATGGCTTGGAAAGACATACCGAGAGAAAGCCAAGAAGGTTCTCCAAGCACTACAGGAGCTTGACCCA
GAGAGTGCTGAGAAGGAGCTAGCAGAGAAGGGCTTCATAGAGGTTGCTGAGGTTAGGATACCGGCTGACCGTGTTAAGAT
TATTGAGAAGGAGGAGAAGGTTACCGGGAAGAGATTCCTACCACACGTCGTAGAACCATCATTTGGTGTTGAGAGGCTGT
TACTAGTAACGCTTGACTACGCCCATCGAGAGCGAGATGGTAGGACTATACTTAGCCTTCCAAGGTACCTGGCTCCGATA
GAGGTTGCAGTGTTTCCGCTGACGCGGGACGAGAAACTCGTAGCTAAGGCTAAGGAGGTGTTTAGAATAGTACTCGATGC
CGGCTTCTACCCAGTCTATGATGACGATGGCAGTATTGGTAAGAGATATGCAAGGGTTGACGAGATCGGAGTACCAGTTG
CTGTAACCGTAGACTACCAGACACTAGAGGACAACACTGTTACGCTAAGGGATAGGGACACCTGGAAGCAAGTAAGAGTT
AGCATTGACAAGCTAGTAGATGCACTTAGAAGATTCCTAAATGGCGCTGACATAGAGGAACTCGGAACCATTGTTGACGC
TGGCGTAGAGACTAAAAGCTAA

Upstream 100 bases:

>100_bases
ATGCACCCCTAGGAGCCTCGTTGCGTGCATTGGCTTTACTAGACGGGGGCGAGTAATATATCCGCGTGTAAAGGGCTCTT
GCTGGGTGAGGGTTGCAACG

Downstream 100 bases:

>100_bases
CTATTTATGTAGTCCTAGCTGATGTGCTATAGTAACTTGGAATAACTAGGGGGCGAGGTGTAGGCTCCCTTTGAGTGAGG
AGCCTAAGATCGAGCAGAAG

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MAKPVDKYEKLVDLAIRRGFFWPSYEIYGGVAGFYDLGPLGVRLKEKILQLWRDFFIRRHAGFVVEIETPIIAPSRVFEA
SGHLEHFTDPIVECRRCGRKFRADHLIEEKLGIKAEGLSIEEMTRLIREHDIRCPVCGGELGEVKKFNLLFKTTIGPYSE
NVGYLRPENAQGMFVAFKRVYEAMRQRLPLGIAQIGRVARNEISPRQGMIRLREFTIAEMEFFFDPEDPGHNGLLDELLG
RVGDTKLRILRAEEKEKGADEPSEYKVSELVEERIVANPWLAYWMAVARDFIKALGVPEQNMYFEEKPPEERAHYSAQTF
DQLVRVSRFGWIEVSGHSYRTDYDLSRHMKHSGQDLRVFKQYREPVIVKRKMIFVDRAWLGKTYREKAKKVLQALQELDP
ESAEKELAEKGFIEVAEVRIPADRVKIIEKEEKVTGKRFLPHVVEPSFGVERLLLVTLDYAHRERDGRTILSLPRYLAPI
EVAVFPLTRDEKLVAKAKEVFRIVLDAGFYPVYDDDGSIGKRYARVDEIGVPVAVTVDYQTLEDNTVTLRDRDTWKQVRV
SIDKLVDALRRFLNGADIEELGTIVDAGVETKS

Sequences:

>Translated_593_residues
MAKPVDKYEKLVDLAIRRGFFWPSYEIYGGVAGFYDLGPLGVRLKEKILQLWRDFFIRRHAGFVVEIETPIIAPSRVFEA
SGHLEHFTDPIVECRRCGRKFRADHLIEEKLGIKAEGLSIEEMTRLIREHDIRCPVCGGELGEVKKFNLLFKTTIGPYSE
NVGYLRPENAQGMFVAFKRVYEAMRQRLPLGIAQIGRVARNEISPRQGMIRLREFTIAEMEFFFDPEDPGHNGLLDELLG
RVGDTKLRILRAEEKEKGADEPSEYKVSELVEERIVANPWLAYWMAVARDFIKALGVPEQNMYFEEKPPEERAHYSAQTF
DQLVRVSRFGWIEVSGHSYRTDYDLSRHMKHSGQDLRVFKQYREPVIVKRKMIFVDRAWLGKTYREKAKKVLQALQELDP
ESAEKELAEKGFIEVAEVRIPADRVKIIEKEEKVTGKRFLPHVVEPSFGVERLLLVTLDYAHRERDGRTILSLPRYLAPI
EVAVFPLTRDEKLVAKAKEVFRIVLDAGFYPVYDDDGSIGKRYARVDEIGVPVAVTVDYQTLEDNTVTLRDRDTWKQVRV
SIDKLVDALRRFLNGADIEELGTIVDAGVETKS
>Mature_592_residues
AKPVDKYEKLVDLAIRRGFFWPSYEIYGGVAGFYDLGPLGVRLKEKILQLWRDFFIRRHAGFVVEIETPIIAPSRVFEAS
GHLEHFTDPIVECRRCGRKFRADHLIEEKLGIKAEGLSIEEMTRLIREHDIRCPVCGGELGEVKKFNLLFKTTIGPYSEN
VGYLRPENAQGMFVAFKRVYEAMRQRLPLGIAQIGRVARNEISPRQGMIRLREFTIAEMEFFFDPEDPGHNGLLDELLGR
VGDTKLRILRAEEKEKGADEPSEYKVSELVEERIVANPWLAYWMAVARDFIKALGVPEQNMYFEEKPPEERAHYSAQTFD
QLVRVSRFGWIEVSGHSYRTDYDLSRHMKHSGQDLRVFKQYREPVIVKRKMIFVDRAWLGKTYREKAKKVLQALQELDPE
SAEKELAEKGFIEVAEVRIPADRVKIIEKEEKVTGKRFLPHVVEPSFGVERLLLVTLDYAHRERDGRTILSLPRYLAPIE
VAVFPLTRDEKLVAKAKEVFRIVLDAGFYPVYDDDGSIGKRYARVDEIGVPVAVTVDYQTLEDNTVTLRDRDTWKQVRVS
IDKLVDALRRFLNGADIEELGTIVDAGVETKS

Specific function: Catalyzes the attachment of glycine to tRNA(Gly) [H]

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI116805340, Length=607, Percent_Identity=33.7726523887974, Blast_Score=341, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI32564541, Length=592, Percent_Identity=36.4864864864865, Blast_Score=358, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI17554996, Length=592, Percent_Identity=36.4864864864865, Blast_Score=357, Evalue=8e-99,
Organism=Saccharomyces cerevisiae, GI6319597, Length=639, Percent_Identity=33.1768388106416, Blast_Score=339, Evalue=9e-94,
Organism=Saccharomyces cerevisiae, GI6325338, Length=601, Percent_Identity=31.9467554076539, Blast_Score=330, Evalue=5e-91,
Organism=Drosophila melanogaster, GI24664462, Length=583, Percent_Identity=36.7066895368782, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI21357965, Length=583, Percent_Identity=36.7066895368782, Blast_Score=371, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022960
- InterPro:   IPR002315 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 68369; Mature: 68237

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPVDKYEKLVDLAIRRGFFWPSYEIYGGVAGFYDLGPLGVRLKEKILQLWRDFFIRRH
CCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHC
AGFVVEIETPIIAPSRVFEASGHLEHFTDPIVECRRCGRKFRADHLIEEKLGIKAEGLSI
CCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EEMTRLIREHDIRCPVCGGELGEVKKFNLLFKTTIGPYSENVGYLRPENAQGMFVAFKRV
HHHHHHHHHCCCCCCCCCCCCCCCHHEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
YEAMRQRLPLGIAQIGRVARNEISPRQGMIRLREFTIAEMEFFFDPEDPGHNGLLDELLG
HHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHH
RVGDTKLRILRAEEKEKGADEPSEYKVSELVEERIVANPWLAYWMAVARDFIKALGVPEQ
HCCCCCEEEEECHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
NMYFEEKPPEERAHYSAQTFDQLVRVSRFGWIEVSGHSYRTDYDLSRHMKHSGQDLRVFK
CCCCCCCCCHHHHHCCHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHCCCCHHHHHH
QYREPVIVKRKMIFVDRAWLGKTYREKAKKVLQALQELDPESAEKELAEKGFIEVAEVRI
HHCCCHHHHHHHHEEHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEC
PADRVKIIEKEEKVTGKRFLPHVVEPSFGVERLLLVTLDYAHRERDGRTILSLPRYLAPI
CHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCE
EVAVFPLTRDEKLVAKAKEVFRIVLDAGFYPVYDDDGSIGKRYARVDEIGVPVAVTVDYQ
EEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEEEEEEEE
TLEDNTVTLRDRDTWKQVRVSIDKLVDALRRFLNGADIEELGTIVDAGVETKS
ECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AKPVDKYEKLVDLAIRRGFFWPSYEIYGGVAGFYDLGPLGVRLKEKILQLWRDFFIRRH
CCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHC
AGFVVEIETPIIAPSRVFEASGHLEHFTDPIVECRRCGRKFRADHLIEEKLGIKAEGLSI
CCEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EEMTRLIREHDIRCPVCGGELGEVKKFNLLFKTTIGPYSENVGYLRPENAQGMFVAFKRV
HHHHHHHHHCCCCCCCCCCCCCCCHHEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
YEAMRQRLPLGIAQIGRVARNEISPRQGMIRLREFTIAEMEFFFDPEDPGHNGLLDELLG
HHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHH
RVGDTKLRILRAEEKEKGADEPSEYKVSELVEERIVANPWLAYWMAVARDFIKALGVPEQ
HCCCCCEEEEECHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
NMYFEEKPPEERAHYSAQTFDQLVRVSRFGWIEVSGHSYRTDYDLSRHMKHSGQDLRVFK
CCCCCCCCCHHHHHCCHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHCCCCHHHHHH
QYREPVIVKRKMIFVDRAWLGKTYREKAKKVLQALQELDPESAEKELAEKGFIEVAEVRI
HHCCCHHHHHHHHEEHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEC
PADRVKIIEKEEKVTGKRFLPHVVEPSFGVERLLLVTLDYAHRERDGRTILSLPRYLAPI
CHHHHHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCE
EVAVFPLTRDEKLVAKAKEVFRIVLDAGFYPVYDDDGSIGKRYARVDEIGVPVAVTVDYQ
EEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEEEEEEEE
TLEDNTVTLRDRDTWKQVRVSIDKLVDALRRFLNGADIEELGTIVDAGVETKS
ECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]