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The map label for this gene is mptN [H]

Identifier: 124026922

GI number: 124026922

Start: 14923

End: 15816

Strand: Reverse

Name: mptN [H]

Synonym: Hbut_0019

Alternate gene names: 124026922

Gene position: 15816-14923 (Counterclockwise)

Preceding gene: 124026923

Following gene: 124026920

Centisome position: 0.95

GC content: 50.34

Gene sequence:

>894_bases
GTGGCTAAAATAGCAGTACTGCACCACACCCCTAGGCCCACGTGGAGTAGCCGACAGTTACTAAAGGCCATAGCCGATAC
GGGCTCGACACCACTCTACATTCTCTGGAACTACATATCTGCAGAACTTGGAACTCCATCATGCCCGTTAAAATATAGAG
GCAGATGCCTCAACGTGGACGCTATCATAGTGCGGGGCCTCGGCAGGGGGCTCAGCATAGAGAGGTATGCGGTGAGGAGA
GCTATTCTAGAGGCAGCGGAATCGTGGGGCTACGTAGTTGTAAACCCGCCAGAGGGACTCTTCCGTGCCAGGGACAAGTT
TACGAGTCTACGAATACTACAGGAGGCGGGGATACCCGTCCCCCGGACACTGGTTACAGAGGATCCTACCACTGCACTAC
ATGCTGTAGAACAGCTCGGTGACGTAGTGTTTAAACCTATAATAGGTAGTCTAGGCCTTGGCTCATTCCGCGTAAAGGAT
ACCGATACGGCCTACCATATCATAAACCTTCTGCTAACCCTCAACCAGCCCCTCTACATACAAAAATACCTGGAAAAGCC
GGGCAATAGGGATCTTCGCGTATTCGTTGTCGGCGACCACGTGGTAGCTGCAATGTACCGCATTGCACCACGAAATAGTT
GGAAAACAAATATAGCTCAAGGAGCAAAACCTGTACCAGCAACAGTCCGTGATGAAGTAGCAAAGGCTGTTATCCGCGCT
GTAAAGGTACTAGGCCTAGTCTACGCCGGTGTAGACGTGATAGAGTATGATGAGAATCGGTATGCAGTCATAGAGGTTAA
TGCCTCACCACTCTGGCGCGGACTACAATCAGCAACTGGGGTAAACCCTGCAAGATACATTGTTGAAAAAGTACTCGAAC
TTGTGAAGAAGTAG

Upstream 100 bases:

>100_bases
CTGGCAGAGATAATTATGCATACCGACCCACTAACAGCACCTGTATACGCGTCACTCATAGCAGAACTCATCGGAGATAT
GCTTAGCGGGCGTGAACGCC

Downstream 100 bases:

>100_bases
GTGGCTCAGCTGTCCTGGCCATCCTCACGGCCTAGAGAGCGTCGGATGAGCCGGTGAAACTCATCACTGCCCGGTGCGCT
ATGCCGCTTTCGGCGCTAGG

Product: glutathione synthase-RimK/ribosomal protein S6 modification (glutaminyl transferase)

Products: NA

Alternate protein names: H(4)MPT:alpha-L-glutamate ligase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MAKIAVLHHTPRPTWSSRQLLKAIADTGSTPLYILWNYISAELGTPSCPLKYRGRCLNVDAIIVRGLGRGLSIERYAVRR
AILEAAESWGYVVVNPPEGLFRARDKFTSLRILQEAGIPVPRTLVTEDPTTALHAVEQLGDVVFKPIIGSLGLGSFRVKD
TDTAYHIINLLLTLNQPLYIQKYLEKPGNRDLRVFVVGDHVVAAMYRIAPRNSWKTNIAQGAKPVPATVRDEVAKAVIRA
VKVLGLVYAGVDVIEYDENRYAVIEVNASPLWRGLQSATGVNPARYIVEKVLELVKK

Sequences:

>Translated_297_residues
MAKIAVLHHTPRPTWSSRQLLKAIADTGSTPLYILWNYISAELGTPSCPLKYRGRCLNVDAIIVRGLGRGLSIERYAVRR
AILEAAESWGYVVVNPPEGLFRARDKFTSLRILQEAGIPVPRTLVTEDPTTALHAVEQLGDVVFKPIIGSLGLGSFRVKD
TDTAYHIINLLLTLNQPLYIQKYLEKPGNRDLRVFVVGDHVVAAMYRIAPRNSWKTNIAQGAKPVPATVRDEVAKAVIRA
VKVLGLVYAGVDVIEYDENRYAVIEVNASPLWRGLQSATGVNPARYIVEKVLELVKK
>Mature_296_residues
AKIAVLHHTPRPTWSSRQLLKAIADTGSTPLYILWNYISAELGTPSCPLKYRGRCLNVDAIIVRGLGRGLSIERYAVRRA
ILEAAESWGYVVVNPPEGLFRARDKFTSLRILQEAGIPVPRTLVTEDPTTALHAVEQLGDVVFKPIIGSLGLGSFRVKDT
DTAYHIINLLLTLNQPLYIQKYLEKPGNRDLRVFVVGDHVVAAMYRIAPRNSWKTNIAQGAKPVPATVRDEVAKAVIRAV
KVLGLVYAGVDVIEYDENRYAVIEVNASPLWRGLQSATGVNPARYIVEKVLELVKK

Specific function: Catalyzes the ATP or GTP-dependent addition of one L- glutamate molecule to tetrahydromethanopterin, producing tetrahydrosarcinapterin [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=218, Percent_Identity=25.6880733944954, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1787076, Length=294, Percent_Identity=28.5714285714286, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789316, Length=184, Percent_Identity=32.0652173913043, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 32915; Mature: 32784

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50975 ATP_GRASP ; PS00141 ASP_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIAVLHHTPRPTWSSRQLLKAIADTGSTPLYILWNYISAELGTPSCPLKYRGRCLNVD
CCEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCCHHHCCEEECHH
AIIVRGLGRGLSIERYAVRRAILEAAESWGYVVVNPPEGLFRARDKFTSLRILQEAGIPV
HHEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCC
PRTLVTEDPTTALHAVEQLGDVVFKPIIGSLGLGSFRVKDTDTAYHIINLLLTLNQPLYI
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCHHH
QKYLEKPGNRDLRVFVVGDHVVAAMYRIAPRNSWKTNIAQGAKPVPATVRDEVAKAVIRA
HHHHCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHH
VKVLGLVYAGVDVIEYDENRYAVIEVNASPLWRGLQSATGVNPARYIVEKVLELVKK
HHHHHHHHHCCEEEEECCCEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKIAVLHHTPRPTWSSRQLLKAIADTGSTPLYILWNYISAELGTPSCPLKYRGRCLNVD
CEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCCHHHCCEEECHH
AIIVRGLGRGLSIERYAVRRAILEAAESWGYVVVNPPEGLFRARDKFTSLRILQEAGIPV
HHEEECCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCC
PRTLVTEDPTTALHAVEQLGDVVFKPIIGSLGLGSFRVKDTDTAYHIINLLLTLNQPLYI
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHCCCCHHH
QKYLEKPGNRDLRVFVVGDHVVAAMYRIAPRNSWKTNIAQGAKPVPATVRDEVAKAVIRA
HHHHCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHH
VKVLGLVYAGVDVIEYDENRYAVIEVNASPLWRGLQSATGVNPARYIVEKVLELVKK
HHHHHHHHHCCEEEEECCCEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12125824 [H]