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The map label for this gene is ispB [H]

Identifier: 124026920

GI number: 124026920

Start: 12983

End: 13921

Strand: Reverse

Name: ispB [H]

Synonym: Hbut_0017

Alternate gene names: 124026920

Gene position: 13921-12983 (Counterclockwise)

Preceding gene: 124026922

Following gene: 124026919

Centisome position: 0.84

GC content: 50.59

Gene sequence:

>939_bases
ATGCAGCAAATAGGCACCAGCTATGGAGGCCTGGTTGAGCACTGGAAGAAGCTCCGGTCACTAGTAGATGATGCTATCAA
GCAGTGGATAGCTCGGCTTCCAGAAGCGCATGCTGTAAGGGTTGCAGAGTATATCGCGAGAGGTGGAAAGCGATTTAGAG
GCTTTCTAGTCCTCGAAATAGCTAGAAGCCTTGGAGGCCGCATAGAAGATGCGCTCGATGCTGCTGTAGCTGTAGAGCTT
ATACACGCCTCAAGCCTTGCACTTGACGACATAATAGACGAGGATGTGGAGCGGAGGGGCCAGAAAGCTGCTTGGGTCGA
GTATGGTGTTAAGAAGACGGTTATGACTGTCTATCTTCTTGTAACATTTGCTCATAAGATAGTCGAGGAAAGGTACGGGT
TTAGAGCTACAAGCCGCACAATAACCGCCACTCTCAACATCTCTCGCGGCGAAGTTCTCGATGCGTTTACGGATCCTGAT
AGTCTTCCCCCAGAATACTATCTTGAAACTGTTAAGCTGAAGACAGCATCCCTCTTCCGCTTAGCTGGCGAGCTTGGCGC
CATTGTTGCAGGCCGCGAAGACCTTTACGGCGAGGCCGGACTCTACGGTGAGTACCTCGGCTTAATGTACCAGGTTGCAG
ACGACATTGTAGACAGCAGGACAGGTACCATCAGTTTCCCCTCGGCGAGAATGTTTAGAAAGTGGCTAGGCAGTGAAGGG
ATAGAGAAAGCTTACAAATTCATAGCAAACCTGCTCACAGAAGCTGAAAAGCTAGCAGAAAAGCTAGCCGCTACACCCGA
CTCCTATCTTCCTATACTACCAGAGTTTATTGTCAGCGCAATGCTTGGTCCAGACATTGAGCGCGTCAAAGCCTATACGT
CCCGGGTAACCCGGTTTAACCCTCCACGTCATGTACCCCGAGGGGATAACCGGGTATGA

Upstream 100 bases:

>100_bases
TAATAATCCACCACGGCAAAACGCCCAGAAACTGGTGCCTGCAAGCATACTGCCAGGGTGCAACCTAGGCTATAACGGCA
CAACTTTGGGTTGATAGCCT

Downstream 100 bases:

>100_bases
CTATTAGGTATGATTACGTAATCATAGGGGCCGGCCCGGCTGGCGCAGCGCTAGCACGTTTCCTAACCGATAAGGGGTTT
AGGGTCAAGGTATATGACAT

Product: geranylgeranyl pyrophosphate synthase, IspA

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MQQIGTSYGGLVEHWKKLRSLVDDAIKQWIARLPEAHAVRVAEYIARGGKRFRGFLVLEIARSLGGRIEDALDAAVAVEL
IHASSLALDDIIDEDVERRGQKAAWVEYGVKKTVMTVYLLVTFAHKIVEERYGFRATSRTITATLNISRGEVLDAFTDPD
SLPPEYYLETVKLKTASLFRLAGELGAIVAGREDLYGEAGLYGEYLGLMYQVADDIVDSRTGTISFPSARMFRKWLGSEG
IEKAYKFIANLLTEAEKLAEKLAATPDSYLPILPEFIVSAMLGPDIERVKAYTSRVTRFNPPRHVPRGDNRV

Sequences:

>Translated_312_residues
MQQIGTSYGGLVEHWKKLRSLVDDAIKQWIARLPEAHAVRVAEYIARGGKRFRGFLVLEIARSLGGRIEDALDAAVAVEL
IHASSLALDDIIDEDVERRGQKAAWVEYGVKKTVMTVYLLVTFAHKIVEERYGFRATSRTITATLNISRGEVLDAFTDPD
SLPPEYYLETVKLKTASLFRLAGELGAIVAGREDLYGEAGLYGEYLGLMYQVADDIVDSRTGTISFPSARMFRKWLGSEG
IEKAYKFIANLLTEAEKLAEKLAATPDSYLPILPEFIVSAMLGPDIERVKAYTSRVTRFNPPRHVPRGDNRV
>Mature_312_residues
MQQIGTSYGGLVEHWKKLRSLVDDAIKQWIARLPEAHAVRVAEYIARGGKRFRGFLVLEIARSLGGRIEDALDAAVAVEL
IHASSLALDDIIDEDVERRGQKAAWVEYGVKKTVMTVYLLVTFAHKIVEERYGFRATSRTITATLNISRGEVLDAFTDPD
SLPPEYYLETVKLKTASLFRLAGELGAIVAGREDLYGEAGLYGEYLGLMYQVADDIVDSRTGTISFPSARMFRKWLGSEG
IEKAYKFIANLLTEAEKLAEKLAATPDSYLPILPEFIVSAMLGPDIERVKAYTSRVTRFNPPRHVPRGDNRV

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=215, Percent_Identity=29.3023255813954, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789578, Length=178, Percent_Identity=32.0224719101124, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786623, Length=285, Percent_Identity=29.8245614035088, Blast_Score=66, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325188, Length=191, Percent_Identity=28.2722513089005, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 34800; Mature: 34800

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQIGTSYGGLVEHWKKLRSLVDDAIKQWIARLPEAHAVRVAEYIARGGKRFRGFLVLEI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHH
ARSLGGRIEDALDAAVAVELIHASSLALDDIIDEDVERRGQKAAWVEYGVKKTVMTVYLL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VTFAHKIVEERYGFRATSRTITATLNISRGEVLDAFTDPDSLPPEYYLETVKLKTASLFR
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
LAGELGAIVAGREDLYGEAGLYGEYLGLMYQVADDIVDSRTGTISFPSARMFRKWLGSEG
HHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCC
IEKAYKFIANLLTEAEKLAEKLAATPDSYLPILPEFIVSAMLGPDIERVKAYTSRVTRFN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
PPRHVPRGDNRV
CCCCCCCCCCCC
>Mature Secondary Structure
MQQIGTSYGGLVEHWKKLRSLVDDAIKQWIARLPEAHAVRVAEYIARGGKRFRGFLVLEI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHH
ARSLGGRIEDALDAAVAVELIHASSLALDDIIDEDVERRGQKAAWVEYGVKKTVMTVYLL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VTFAHKIVEERYGFRATSRTITATLNISRGEVLDAFTDPDSLPPEYYLETVKLKTASLFR
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
LAGELGAIVAGREDLYGEAGLYGEYLGLMYQVADDIVDSRTGTISFPSARMFRKWLGSEG
HHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCC
IEKAYKFIANLLTEAEKLAEKLAATPDSYLPILPEFIVSAMLGPDIERVKAYTSRVTRFN
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
PPRHVPRGDNRV
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]