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The map label for this gene is dnaB [H]

Identifier: 124026849

GI number: 124026849

Start: 1801292

End: 1802710

Strand: Direct

Name: dnaB [H]

Synonym: NATL1_21441

Alternate gene names: 124026849

Gene position: 1801292-1802710 (Clockwise)

Preceding gene: 124026848

Following gene: 124026850

Centisome position: 96.6

GC content: 38.48

Gene sequence:

>1419_bases
ATGATAAGCACCCCCTCTTCAAATAACGGGGATTTTTCAAAGCAAGGTAGAGATTTTGGAGCTTTTAATAATTCCAAGAT
TGAGAATCCTCGTTTTGAAGCTCAACCTGACCAAATACCACCACAAAATTTAGAAGCAGAAGAATCTGTTCTTGGTGGAA
TTTTATTGGATCCTGATGCGATAGGACGAATAGCTGATTTGCTACAAGTTGAAGCTTTTTACATATCTGCTCATCGAGAA
ATATATAGAACTGCATTAATGCTTCATAGCCAGGGCAAGCCTACAGATTTAACAGCAATGAGCGCTTGGCTTGCGGATAC
AAATTCTTTAGAAAAAGTTGGAGGCAACAACCGATTAGTTGAACTTGTTGAGCGGGTTACATCTACAGCTTCAATCGAAC
AAGTTGCTAAATTAATTAGCGATAAATTTCTGCGCAGACAATTAATTAAATCTGGAAATGATGTAATCAAATTAGGCTTT
GATCAAAGTCTGCCCATGGAAGAAGCCATTGATCAAGCTGAACAAAAGATCTTTGCTATTAGCCAAGAGCAACCATCCAA
AGGTCTAACACCCACAGCAGAAATTTTGACAAGTACTTTCAATGAAATAGAGAGTAGATCACTTGGTACATCAGTAGCAG
GCATACCAGTTAATTTTTACGATCTGGATGCAATGACACAAGGACTGCAGCGGAGCGATCTAATAATTGTTGCAGGAAGA
CCAGCAATGGGAAAAACTTCAATTGTTCTTAATTTGGCTAAAAACGTTGCACAACTACATGACTTACCTGTTTGTGTATT
CAGTCTTGAGATGAGTAAAGAGCAACTAACTTACAGATTGCTATCAAGCGAGGTAGGTATTGAGAGCAGTAGATTAAGAA
CGGGACGTTTGCAACAAGATGAATGGCCATTACTTGGTCAAGGTATTAATACGCTTGGTCAATTACCAATCTTCATTGAT
GACAAGCCAAATTCCAGTGTTCTTGAGATGAGGTCCTTATGTCGTCGCCTAATTGCTGAACAAGGTAAAGAGCTTGGACT
CATTGTGATTGATTATCTTCAACTAATGGAAGGTACTTCTCCAGATAATCGAGTTCAAGAAATTTCAAGGATTACTAGAG
GCCTTAAGGGTATGGCAAGAGAACTTAAAGTGCCTGTAATCGCTTTATCTCAATTAAGTAGAGGAGTTGAATCAAGAACC
AATAAAAGACCAATGCTTAGCGATCTGCGTGAATCTGGCTCAATAGAACAGGATGCGGACTTGGTTCTAATGATTTATAG
AGATGAATATTACAATCCAGAAACTACTGATAGAGGAATTACAGAAGTTATCGTGACAAAGCACCGGAATGGACCCGTTG
GAACAGTTAAATTACTTTTTGAACCCCAATTCACTAGATTTAGAAATCTTGCTGCTTAA

Upstream 100 bases:

>100_bases
CGAAGTAAATGCTGAGATAAACCTTGAAGTTACAAGTTACTAATAGTTGCATCTTTGCCTGAACAAGCCAAAGTAGGTGT
CCTTGTCCAAAAAGCGATAA

Downstream 100 bases:

>100_bases
TTGTTTGAAAATTTATTTCCTAAATGCTTAATTGATGATTACTAAACAATCTTCAAATGAAAGTTTTGACGTAATTGTTG
TAGGAGGTGGTCATGCAGGC

Product: DnaB replicative helicase

Products: NA

Alternate protein names: Asp dnaB intein [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MISTPSSNNGDFSKQGRDFGAFNNSKIENPRFEAQPDQIPPQNLEAEESVLGGILLDPDAIGRIADLLQVEAFYISAHRE
IYRTALMLHSQGKPTDLTAMSAWLADTNSLEKVGGNNRLVELVERVTSTASIEQVAKLISDKFLRRQLIKSGNDVIKLGF
DQSLPMEEAIDQAEQKIFAISQEQPSKGLTPTAEILTSTFNEIESRSLGTSVAGIPVNFYDLDAMTQGLQRSDLIIVAGR
PAMGKTSIVLNLAKNVAQLHDLPVCVFSLEMSKEQLTYRLLSSEVGIESSRLRTGRLQQDEWPLLGQGINTLGQLPIFID
DKPNSSVLEMRSLCRRLIAEQGKELGLIVIDYLQLMEGTSPDNRVQEISRITRGLKGMARELKVPVIALSQLSRGVESRT
NKRPMLSDLRESGSIEQDADLVLMIYRDEYYNPETTDRGITEVIVTKHRNGPVGTVKLLFEPQFTRFRNLAA

Sequences:

>Translated_472_residues
MISTPSSNNGDFSKQGRDFGAFNNSKIENPRFEAQPDQIPPQNLEAEESVLGGILLDPDAIGRIADLLQVEAFYISAHRE
IYRTALMLHSQGKPTDLTAMSAWLADTNSLEKVGGNNRLVELVERVTSTASIEQVAKLISDKFLRRQLIKSGNDVIKLGF
DQSLPMEEAIDQAEQKIFAISQEQPSKGLTPTAEILTSTFNEIESRSLGTSVAGIPVNFYDLDAMTQGLQRSDLIIVAGR
PAMGKTSIVLNLAKNVAQLHDLPVCVFSLEMSKEQLTYRLLSSEVGIESSRLRTGRLQQDEWPLLGQGINTLGQLPIFID
DKPNSSVLEMRSLCRRLIAEQGKELGLIVIDYLQLMEGTSPDNRVQEISRITRGLKGMARELKVPVIALSQLSRGVESRT
NKRPMLSDLRESGSIEQDADLVLMIYRDEYYNPETTDRGITEVIVTKHRNGPVGTVKLLFEPQFTRFRNLAA
>Mature_472_residues
MISTPSSNNGDFSKQGRDFGAFNNSKIENPRFEAQPDQIPPQNLEAEESVLGGILLDPDAIGRIADLLQVEAFYISAHRE
IYRTALMLHSQGKPTDLTAMSAWLADTNSLEKVGGNNRLVELVERVTSTASIEQVAKLISDKFLRRQLIKSGNDVIKLGF
DQSLPMEEAIDQAEQKIFAISQEQPSKGLTPTAEILTSTFNEIESRSLGTSVAGIPVNFYDLDAMTQGLQRSDLIIVAGR
PAMGKTSIVLNLAKNVAQLHDLPVCVFSLEMSKEQLTYRLLSSEVGIESSRLRTGRLQQDEWPLLGQGINTLGQLPIFID
DKPNSSVLEMRSLCRRLIAEQGKELGLIVIDYLQLMEGTSPDNRVQEISRITRGLKGMARELKVPVIALSQLSRGVESRT
NKRPMLSDLRESGSIEQDADLVLMIYRDEYYNPETTDRGITEVIVTKHRNGPVGTVKLLFEPQFTRFRNLAA

Specific function: The intein is an endonuclease (Potential) [H]

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=462, Percent_Identity=41.991341991342, Blast_Score=344, Evalue=9e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR003586
- InterPro:   IPR003587
- InterPro:   IPR006142
- InterPro:   IPR004042
- InterPro:   IPR006141 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52401; Mature: 52401

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISTPSSNNGDFSKQGRDFGAFNNSKIENPRFEAQPDQIPPQNLEAEESVLGGILLDPDA
CCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEECHHH
IGRIADLLQVEAFYISAHREIYRTALMLHSQGKPTDLTAMSAWLADTNSLEKVGGNNRLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHCCCHHHH
ELVERVTSTASIEQVAKLISDKFLRRQLIKSGNDVIKLGFDQSLPMEEAIDQAEQKIFAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHEEEE
SQEQPSKGLTPTAEILTSTFNEIESRSLGTSVAGIPVNFYDLDAMTQGLQRSDLIIVAGR
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHCCCCCCEEEEECC
PAMGKTSIVLNLAKNVAQLHDLPVCVFSLEMSKEQLTYRLLSSEVGIESSRLRTGRLQQD
CCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
EWPLLGQGINTLGQLPIFIDDKPNSSVLEMRSLCRRLIAEQGKELGLIVIDYLQLMEGTS
CCCCHHHCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
PDNRVQEISRITRGLKGMARELKVPVIALSQLSRGVESRTNKRPMLSDLRESGSIEQDAD
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCC
LVLMIYRDEYYNPETTDRGITEVIVTKHRNGPVGTVKLLFEPQFTRFRNLAA
EEEEEEECCCCCCCCCCCCHHHHHEEECCCCCCEEEEEEECCCHHHHHHCCC
>Mature Secondary Structure
MISTPSSNNGDFSKQGRDFGAFNNSKIENPRFEAQPDQIPPQNLEAEESVLGGILLDPDA
CCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEECHHH
IGRIADLLQVEAFYISAHREIYRTALMLHSQGKPTDLTAMSAWLADTNSLEKVGGNNRLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHCCCHHHH
ELVERVTSTASIEQVAKLISDKFLRRQLIKSGNDVIKLGFDQSLPMEEAIDQAEQKIFAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHEEEE
SQEQPSKGLTPTAEILTSTFNEIESRSLGTSVAGIPVNFYDLDAMTQGLQRSDLIIVAGR
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHCCCCCCEEEEECC
PAMGKTSIVLNLAKNVAQLHDLPVCVFSLEMSKEQLTYRLLSSEVGIESSRLRTGRLQQD
CCCCCHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
EWPLLGQGINTLGQLPIFIDDKPNSSVLEMRSLCRRLIAEQGKELGLIVIDYLQLMEGTS
CCCCHHHCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
PDNRVQEISRITRGLKGMARELKVPVIALSQLSRGVESRTNKRPMLSDLRESGSIEQDAD
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCC
LVLMIYRDEYYNPETTDRGITEVIVTKHRNGPVGTVKLLFEPQFTRFRNLAA
EEEEEEECCCCCCCCCCCCHHHHHEEECCCCCCEEEEEEECCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]