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The map label for this gene is rpoD3 [H]

Identifier: 124026787

GI number: 124026787

Start: 1725107

End: 1726114

Strand: Reverse

Name: rpoD3 [H]

Synonym: NATL1_20821

Alternate gene names: 124026787

Gene position: 1726114-1725107 (Counterclockwise)

Preceding gene: 124026788

Following gene: 124026785

Centisome position: 92.57

GC content: 38.99

Gene sequence:

>1008_bases
ATGACAACGGCAGTAGAGGCTCCTAAGACTAAGTATTCATCTTCCGCTGCTTCTGCAAGATCCGTTACGGATATTGACTT
AGTCCGTTCTTACTTGAGAGATATTGGAAGAGTCCCTTTGCTCTCACACGAGCAGGAAATTACCTTGGGAAGACAAGTTC
AAGATCTCGTTGTCCTTGAAAATCTTGAGACCGAACTCGAGAGTGATTTAGGGGAAAAGCCAGACATCAACTTTTTCGCT
GACAAGGCTGGACTTTCTCCGATCCAATTAAAAAAGAAGCTGAAGAGTGGTAGAAGAGCAAAAGAGAGAATGGTTGCAGC
CAATCTTCGTTTGGTAGTAAGTGTTGCTAAGAAATACACAAAAAGAAATATGGAATTATTAGATTTAATTCAAGAAGGAA
CTATCGGCTTAGTTCGGGGAGTTGAAAAATTTGATCCCACTCGTGGATACAAGTTTTCAACCTATGCCTACTGGTGGATA
CGTCAAGGCATCACACGCGCAATTGCTGAAAAAAGTAGATCCATAAGATTGCCTATACACATAACTGAAATGCTAAACAA
GTTGAAAAAAGGTCAACGAGAATTAAGTCAAGAACTTTCTAGAACTCCAACCTTGAAAGAACTATCTGAGTATGTTGAGT
TGCCCGAGACTGACGTTAAAGATTTAATGAGTAGAGCTGGGCAGCCAGTTAGCTTAGAAACAAAAATTGGCGATGGCGAA
GATACTATTTTATTAGATTTATTATCTAACGAAATTGATATGCCCTCCGAGCAAATTGAAAGTGATTGCATGAAGGGTGA
TTTAGAAACATTACTCGAACAATTACCCGAATTACAAAATCGTGTTTTAAGAATGAGATACGGTATGGATGGTGATGATC
CTATGAGTCTTACTGGTATTGGAAGAGTTTTGGGTATTAGTAGAGATAGAGTAAGAAATTTAGAAAGAGATGGTTTGCGA
GGTTTAAGAAAAACCGGAGACTCAGTTGAAGCTTATATGGCTTCTTGA

Upstream 100 bases:

>100_bases
AAGTCGAATGAGTGTTTTTACTCAAAGTGTGGGAAGCCGAGCTATTCTTGTTTGATCTTTACAATTCTTAGTAGTAAGCT
TTACAAAACTCAACAAACCT

Downstream 100 bases:

>100_bases
ATTATCCTTATTAGAACAGGATTAACTTTTTCTGGCATTTCATCATGGGGACAATGCCCTGCGTCTGAAATAACATCTAA
AGATTTTATACATTCAAAAG

Product: type II alternative sigma-70 family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTTAVEAPKTKYSSSAASARSVTDIDLVRSYLRDIGRVPLLSHEQEITLGRQVQDLVVLENLETELESDLGEKPDINFFA
DKAGLSPIQLKKKLKSGRRAKERMVAANLRLVVSVAKKYTKRNMELLDLIQEGTIGLVRGVEKFDPTRGYKFSTYAYWWI
RQGITRAIAEKSRSIRLPIHITEMLNKLKKGQRELSQELSRTPTLKELSEYVELPETDVKDLMSRAGQPVSLETKIGDGE
DTILLDLLSNEIDMPSEQIESDCMKGDLETLLEQLPELQNRVLRMRYGMDGDDPMSLTGIGRVLGISRDRVRNLERDGLR
GLRKTGDSVEAYMAS

Sequences:

>Translated_335_residues
MTTAVEAPKTKYSSSAASARSVTDIDLVRSYLRDIGRVPLLSHEQEITLGRQVQDLVVLENLETELESDLGEKPDINFFA
DKAGLSPIQLKKKLKSGRRAKERMVAANLRLVVSVAKKYTKRNMELLDLIQEGTIGLVRGVEKFDPTRGYKFSTYAYWWI
RQGITRAIAEKSRSIRLPIHITEMLNKLKKGQRELSQELSRTPTLKELSEYVELPETDVKDLMSRAGQPVSLETKIGDGE
DTILLDLLSNEIDMPSEQIESDCMKGDLETLLEQLPELQNRVLRMRYGMDGDDPMSLTGIGRVLGISRDRVRNLERDGLR
GLRKTGDSVEAYMAS
>Mature_334_residues
TTAVEAPKTKYSSSAASARSVTDIDLVRSYLRDIGRVPLLSHEQEITLGRQVQDLVVLENLETELESDLGEKPDINFFAD
KAGLSPIQLKKKLKSGRRAKERMVAANLRLVVSVAKKYTKRNMELLDLIQEGTIGLVRGVEKFDPTRGYKFSTYAYWWIR
QGITRAIAEKSRSIRLPIHITEMLNKLKKGQRELSQELSRTPTLKELSEYVELPETDVKDLMSRAGQPVSLETKIGDGED
TILLDLLSNEIDMPSEQIESDCMKGDLETLLEQLPELQNRVLRMRYGMDGDDPMSLTGIGRVLGISRDRVRNLERDGLRG
LRKTGDSVEAYMAS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=233, Percent_Identity=44.6351931330472, Blast_Score=221, Evalue=4e-59,
Organism=Escherichia coli, GI1789098, Length=301, Percent_Identity=36.8770764119601, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1789871, Length=234, Percent_Identity=26.0683760683761, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788231, Length=203, Percent_Identity=27.0935960591133, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 37801; Mature: 37670

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAVEAPKTKYSSSAASARSVTDIDLVRSYLRDIGRVPLLSHEQEITLGRQVQDLVVLE
CCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
NLETELESDLGEKPDINFFADKAGLSPIQLKKKLKSGRRAKERMVAANLRLVVSVAKKYT
HHHHHHHHHCCCCCCCCEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRNMELLDLIQEGTIGLVRGVEKFDPTRGYKFSTYAYWWIRQGITRAIAEKSRSIRLPIH
HCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCEE
ITEMLNKLKKGQRELSQELSRTPTLKELSEYVELPETDVKDLMSRAGQPVSLETKIGDGE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCC
DTILLDLLSNEIDMPSEQIESDCMKGDLETLLEQLPELQNRVLRMRYGMDGDDPMSLTGI
CHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
GRVLGISRDRVRNLERDGLRGLRKTGDSVEAYMAS
HHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
TTAVEAPKTKYSSSAASARSVTDIDLVRSYLRDIGRVPLLSHEQEITLGRQVQDLVVLE
CCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
NLETELESDLGEKPDINFFADKAGLSPIQLKKKLKSGRRAKERMVAANLRLVVSVAKKYT
HHHHHHHHHCCCCCCCCEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRNMELLDLIQEGTIGLVRGVEKFDPTRGYKFSTYAYWWIRQGITRAIAEKSRSIRLPIH
HCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCEE
ITEMLNKLKKGQRELSQELSRTPTLKELSEYVELPETDVKDLMSRAGQPVSLETKIGDGE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCC
DTILLDLLSNEIDMPSEQIESDCMKGDLETLLEQLPELQNRVLRMRYGMDGDDPMSLTGI
CHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
GRVLGISRDRVRNLERDGLRGLRKTGDSVEAYMAS
HHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA