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The map label for this gene is nadE [H]

Identifier: 124026550

GI number: 124026550

Start: 1507085

End: 1508794

Strand: Direct

Name: nadE [H]

Synonym: NATL1_18451

Alternate gene names: 124026550

Gene position: 1507085-1508794 (Clockwise)

Preceding gene: 124026549

Following gene: 124026562

Centisome position: 80.82

GC content: 35.2

Gene sequence:

>1710_bases
ATGAAATTAGCTCTGGCCCAACTAAATCCAGTTATTGGAGATCTCGATGGTAATTCCGAGAAAATATTTGAAGTATGCGA
AGAGATCAAAGACAAGGATGTGGATCTAGTAATAACACCAGAGCTTTCTTTAATCGGCTACCCTCCAAAAGACTTATTAT
TTAATCCAAGACTTTTTGAAGAGGAAAAAGATGCCCTAAATAAATTAAGCAAAAGATTAAGTAATCTTAGTCAAAATTTA
TCTGTATTAATTGGTATTGCAGAACCAACTCCAGATATAGAAATTCCTAAACTCTACAATTCAGTTGTGCTTTTAGAAAA
AGGTACCTGGAGAATCGTAGCCAGGAAGCAACTCCTACCTACTTACGATGTATTTGATGAAAAACGTTATTTTCGTTCAG
CAGAGAATAGTAGTATTTTAAGTTTTAATTTTCAAAAAAAACTTTGGAAGATAGGAATCACTATTTGTGAAGATATATGG
GTTGAGCAAAATCTACAAAATGAAAAGATCCTAGGGAAAGATCCTTTAAAATTCTTAGAAAACGAAAAATTAGATTTGCT
TATCAATCTTTCAGCTTCTCCATTTATTGAATCGAAAAGTTTAATACGTCAACGCATAGCAGCTAAGGCAGCCATGCGTC
TTTCATGCCCAGTGATCTATGTCAATCAAGTAGGAGGGAATGATGAATTGATCTTTGACGGATCTAGTTTTGCGATTAAT
AAAGAAGGCGAGTTAAAACAAGAACTTCCTAGTTTTAAAGAATCAGTTGGTCTTTGTGATATATCTTCCCTCAAGCAACA
ACCTTCGATATTAAGTAAATACCCAACATCACAAGAAGTCATTTTCAGGGCTCTAGTGCTTGGAGTTAAAGATTATGCAA
GAAAATGTAATTTCCACAGTGCCGTTATTGGATTAAGTGGGGGTATTGACTCAGCCCTAGTAGCAACTATTGCAGTAGCA
GCTTTAGGTATCTCTAAAGTTCATGCGATTTTAATGCCTTCTCCATGGAGTTCAGCAGGGTCCGTAAAAGATGCAACCAC
ATTAGCTAATCGACTGGGAATAAATCATCAAAAGATTCCGATCTCAGATGTAATGAATAGTTTTAACGATGTTTTATCCA
ATTCAATATGGGGAATACCAATCGGTATCACAGCTGAGAATCTACAATCTCGCATTAGGGGAACAATATTGATGGCCATA
GCAAATCAAAAAAAATATTTACTTCTATCAACAGGAAATAAGTCAGAACTTGCCGTAGGATATTGCACACTTTACGGAGA
CATGAATGGAGGCTTGTCTGTAATTGGAGATCTTTATAAGACAAGTGTATTTGATCTATGTGATTGGATAGATAGTGAAT
CGTCATCTAGTTGTAGAAATGATTTTTCTCTCCCTGAAAAAGGAGAAATTATAAGCTCTGAGATAAGAGACAAGCCTCCA
AGTGCTGAATTACGGCCTGAACAATTAGATAGCGACTCTTTGCCTGAGTATTCCATTCTTGATCAAATACTAAAAGGATT
AATTGAACAGCACTTACCAACTGAGGTGTTAATAGAAAAAGGATTTGATAAAAAAATTGTTCATAAAGTAGCCAATCTTT
TAAAGAACGCTGAATTCAAACGTTATCAAGCACCTCCTTTATTGAAAATTAGTAATCAAGCATTTGGAAGTGGGTGGAAA
AAACCTATAGCCTCAGGACAAATCCTTTAA

Upstream 100 bases:

>100_bases
GTTCAAAATTTAGAGAGAGACCTCAAAAAATACTTGTTCCTAAGGAGCTTGTCCCATTACTGCTTGAAGAAAATCTATAC
GGTCTAGCAGATAACAGAAA

Downstream 100 bases:

>100_bases
TTAACTTCTATTCGTCATTTAATTGCTTTAAACAATCAATTGGTCTTCGCAGAGACTTAACATTAATCGCATGACGTAAA
GCTGTGATTAATCCTGCAGC

Product: carbon-nitrogen hydrolase:NAD+ synthase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MKLALAQLNPVIGDLDGNSEKIFEVCEEIKDKDVDLVITPELSLIGYPPKDLLFNPRLFEEEKDALNKLSKRLSNLSQNL
SVLIGIAEPTPDIEIPKLYNSVVLLEKGTWRIVARKQLLPTYDVFDEKRYFRSAENSSILSFNFQKKLWKIGITICEDIW
VEQNLQNEKILGKDPLKFLENEKLDLLINLSASPFIESKSLIRQRIAAKAAMRLSCPVIYVNQVGGNDELIFDGSSFAIN
KEGELKQELPSFKESVGLCDISSLKQQPSILSKYPTSQEVIFRALVLGVKDYARKCNFHSAVIGLSGGIDSALVATIAVA
ALGISKVHAILMPSPWSSAGSVKDATTLANRLGINHQKIPISDVMNSFNDVLSNSIWGIPIGITAENLQSRIRGTILMAI
ANQKKYLLLSTGNKSELAVGYCTLYGDMNGGLSVIGDLYKTSVFDLCDWIDSESSSSCRNDFSLPEKGEIISSEIRDKPP
SAELRPEQLDSDSLPEYSILDQILKGLIEQHLPTEVLIEKGFDKKIVHKVANLLKNAEFKRYQAPPLLKISNQAFGSGWK
KPIASGQIL

Sequences:

>Translated_569_residues
MKLALAQLNPVIGDLDGNSEKIFEVCEEIKDKDVDLVITPELSLIGYPPKDLLFNPRLFEEEKDALNKLSKRLSNLSQNL
SVLIGIAEPTPDIEIPKLYNSVVLLEKGTWRIVARKQLLPTYDVFDEKRYFRSAENSSILSFNFQKKLWKIGITICEDIW
VEQNLQNEKILGKDPLKFLENEKLDLLINLSASPFIESKSLIRQRIAAKAAMRLSCPVIYVNQVGGNDELIFDGSSFAIN
KEGELKQELPSFKESVGLCDISSLKQQPSILSKYPTSQEVIFRALVLGVKDYARKCNFHSAVIGLSGGIDSALVATIAVA
ALGISKVHAILMPSPWSSAGSVKDATTLANRLGINHQKIPISDVMNSFNDVLSNSIWGIPIGITAENLQSRIRGTILMAI
ANQKKYLLLSTGNKSELAVGYCTLYGDMNGGLSVIGDLYKTSVFDLCDWIDSESSSSCRNDFSLPEKGEIISSEIRDKPP
SAELRPEQLDSDSLPEYSILDQILKGLIEQHLPTEVLIEKGFDKKIVHKVANLLKNAEFKRYQAPPLLKISNQAFGSGWK
KPIASGQIL
>Mature_569_residues
MKLALAQLNPVIGDLDGNSEKIFEVCEEIKDKDVDLVITPELSLIGYPPKDLLFNPRLFEEEKDALNKLSKRLSNLSQNL
SVLIGIAEPTPDIEIPKLYNSVVLLEKGTWRIVARKQLLPTYDVFDEKRYFRSAENSSILSFNFQKKLWKIGITICEDIW
VEQNLQNEKILGKDPLKFLENEKLDLLINLSASPFIESKSLIRQRIAAKAAMRLSCPVIYVNQVGGNDELIFDGSSFAIN
KEGELKQELPSFKESVGLCDISSLKQQPSILSKYPTSQEVIFRALVLGVKDYARKCNFHSAVIGLSGGIDSALVATIAVA
ALGISKVHAILMPSPWSSAGSVKDATTLANRLGINHQKIPISDVMNSFNDVLSNSIWGIPIGITAENLQSRIRGTILMAI
ANQKKYLLLSTGNKSELAVGYCTLYGDMNGGLSVIGDLYKTSVFDLCDWIDSESSSSCRNDFSLPEKGEIISSEIRDKPP
SAELRPEQLDSDSLPEYSILDQILKGLIEQHLPTEVLIEKGFDKKIVHKVANLLKNAEFKRYQAPPLLKISNQAFGSGWK
KPIASGQIL

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 63011; Mature: 63011

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLALAQLNPVIGDLDGNSEKIFEVCEEIKDKDVDLVITPELSLIGYPPKDLLFNPRLFE
CCEEHHHCCCCEECCCCCHHHHHHHHHHHCCCCCCEEECCCCEEECCCCHHHCCCCHHHH
EEKDALNKLSKRLSNLSQNLSVLIGIAEPTPDIEIPKLYNSVVLLEKGTWRIVARKQLLP
HHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHCCEEEEECCCEEEEEHHHCCC
TYDVFDEKRYFRSAENSSILSFNFQKKLWKIGITICEDIWVEQNLQNEKILGKDPLKFLE
CHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHC
NEKLDLLINLSASPFIESKSLIRQRIAAKAAMRLSCPVIYVNQVGGNDELIFDGSSFAIN
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCEEEC
KEGELKQELPSFKESVGLCDISSLKQQPSILSKYPTSQEVIFRALVLGVKDYARKCNFHS
CCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
AVIGLSGGIDSALVATIAVAALGISKVHAILMPSPWSSAGSVKDATTLANRLGINHQKIP
EEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCC
ISDVMNSFNDVLSNSIWGIPIGITAENLQSRIRGTILMAIANQKKYLLLSTGNKSELAVG
HHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHEEEEEEECCCEEEEEECCCCCCEEEE
YCTLYGDMNGGLSVIGDLYKTSVFDLCDWIDSESSSSCRNDFSLPEKGEIISSEIRDKPP
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCHHHHHHCCCCC
SAELRPEQLDSDSLPEYSILDQILKGLIEQHLPTEVLIEKGFDKKIVHKVANLLKNAEFK
CCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHCCCCH
RYQAPPLLKISNQAFGSGWKKPIASGQIL
HCCCCCCEEECCCHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLALAQLNPVIGDLDGNSEKIFEVCEEIKDKDVDLVITPELSLIGYPPKDLLFNPRLFE
CCEEHHHCCCCEECCCCCHHHHHHHHHHHCCCCCCEEECCCCEEECCCCHHHCCCCHHHH
EEKDALNKLSKRLSNLSQNLSVLIGIAEPTPDIEIPKLYNSVVLLEKGTWRIVARKQLLP
HHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHCCEEEEECCCEEEEEHHHCCC
TYDVFDEKRYFRSAENSSILSFNFQKKLWKIGITICEDIWVEQNLQNEKILGKDPLKFLE
CHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHC
NEKLDLLINLSASPFIESKSLIRQRIAAKAAMRLSCPVIYVNQVGGNDELIFDGSSFAIN
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCEEEC
KEGELKQELPSFKESVGLCDISSLKQQPSILSKYPTSQEVIFRALVLGVKDYARKCNFHS
CCCCHHHHHHHHHHHCCCCCHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
AVIGLSGGIDSALVATIAVAALGISKVHAILMPSPWSSAGSVKDATTLANRLGINHQKIP
EEEECCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCC
ISDVMNSFNDVLSNSIWGIPIGITAENLQSRIRGTILMAIANQKKYLLLSTGNKSELAVG
HHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHEEEEEEECCCEEEEEECCCCCCEEEE
YCTLYGDMNGGLSVIGDLYKTSVFDLCDWIDSESSSSCRNDFSLPEKGEIISSEIRDKPP
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCHHHHHHCCCCC
SAELRPEQLDSDSLPEYSILDQILKGLIEQHLPTEVLIEKGFDKKIVHKVANLLKNAEFK
CCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHCCCCH
RYQAPPLLKISNQAFGSGWKKPIASGQIL
HCCCCCCEEECCCHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]