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The map label for this gene is purD [H]

Identifier: 124026472

GI number: 124026472

Start: 1448378

End: 1449682

Strand: Direct

Name: purD [H]

Synonym: NATL1_17671

Alternate gene names: 124026472

Gene position: 1448378-1449682 (Clockwise)

Preceding gene: 124026464

Following gene: 124026473

Centisome position: 77.67

GC content: 37.47

Gene sequence:

>1305_bases
ATGAAAAAGAATCAAAAAGCTGAAGAATTGAAAAGGATATTAATAGTTGGAAGCGGAGGTAGAGAAAACTCTATTGCTTG
GGCTCTATCAAAAAATCAATCTATTGAGCAAATATATGTTTGTCCTGGAAATGGTGGGACAGCCAAATTTGAAAAATGTA
TTTGCCTAAAACCCAACTCTGAAGATGAAAAAATAATCATTAATGAGTGTAAGCGACTTGCCATTGATTTAGTCATCATT
GGTCCTGAAGACCCTTTGGCTGAGGGCTTAGCAAATAAAATGCGTGAGGCAGGGTTAACAGTTTTTGGTCCAGGCAAAGA
TGGTGCTCAATTAGAGGCAAGTAAAGACTGGTCTAAGGCTCTGATGATAGAGAACAATATCCCTACAGCAAAGTACTGGT
CTGCAAAATCTAAAGAAGAAGCACTAGAAATTCTTAAAAGATTTAACCAACCTCTTGTTATCAAAGCAGATGGTCTTGCG
GCAGGTAAAGGTGTCACTGTTTGTGAAACCATTGAGGAATCTAAGGAAGCTATTAAGGACATATTTTCTGGAAAGTTTGG
CTCTGCAGGAAATAAAGTTATTCTCGAAGAAAAAATTGAAGGGCCAGAAGTTTCTATTTTTGCCCTTTGTGATGGAGAGA
AATTAATTGTTCTTCCTCCAGCCCAAGATCATAAAAGATTACTTGATGGAGACAATGGTCCAAATACTGGCGGAATGGGA
GCTTATGCACCTGCACTTTTAATTAATGAACAAGATCTTAAGGACTTAACTGAACTGGTTCTTATCCCAACTTTAAAAGG
TTTGAAAAAAAAGAATATCAATTATATCGGTGTCATTTATGCCGGTTTAATGCTTACATCGTCAGGGCCAAAAGTTATTG
AATTCAATTGCCGCTTTGGAGATCCAGAATGTCAAGCTTTAATGCCCTTAATGGGAGAGGAATTTGCTTCTGTCCTTTTT
GCTTGCGCTCGAGGAGAGATTGAGAATGCACCAAAACTTACATTTAACTCTGAATGCAGTGCTTGTATAGTTGCAGCCTC
TAAGGGATACCCTGAAAGCCCACAAAAAGGTGAAAAGATTGCCATCAATGTTGAATCAAATTCTTCACTCCAAATTTTTC
ACGCAGGCACCACAATTGACAAATTTGACAATATAATTACCTCAGGTGGAAGAGTTCTTTCAGTAGTTGCTCAGGGAGAA
AGTTTCGACAAAGCTTTCGATTTAGCCTATTCTAACCTTAAAAAAATTAACTTTAATGGTATGCATTTTCGCGAAGATAT
AGGTTACCAAGTTAGGAATATTTGA

Upstream 100 bases:

>100_bases
ACAAATAAATACCTTTTTCAAGCAATGATATGAACTAATTAATCAAACGGTAATTAAAAATCTCTAAAAACTATTTTTTC
ATTTAAGCAAGAGTAAAACT

Downstream 100 bases:

>100_bases
ATTAATTTATCTTATATGAATAATACTAATTTAAATCAGAATAAAAACTTTATAGCATCAAAACATGATGTAATATTAGA
TGATTTAACCTTTTTAGAAA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MKKNQKAEELKRILIVGSGGRENSIAWALSKNQSIEQIYVCPGNGGTAKFEKCICLKPNSEDEKIIINECKRLAIDLVII
GPEDPLAEGLANKMREAGLTVFGPGKDGAQLEASKDWSKALMIENNIPTAKYWSAKSKEEALEILKRFNQPLVIKADGLA
AGKGVTVCETIEESKEAIKDIFSGKFGSAGNKVILEEKIEGPEVSIFALCDGEKLIVLPPAQDHKRLLDGDNGPNTGGMG
AYAPALLINEQDLKDLTELVLIPTLKGLKKKNINYIGVIYAGLMLTSSGPKVIEFNCRFGDPECQALMPLMGEEFASVLF
ACARGEIENAPKLTFNSECSACIVAASKGYPESPQKGEKIAINVESNSSLQIFHAGTTIDKFDNIITSGGRVLSVVAQGE
SFDKAFDLAYSNLKKINFNGMHFREDIGYQVRNI

Sequences:

>Translated_434_residues
MKKNQKAEELKRILIVGSGGRENSIAWALSKNQSIEQIYVCPGNGGTAKFEKCICLKPNSEDEKIIINECKRLAIDLVII
GPEDPLAEGLANKMREAGLTVFGPGKDGAQLEASKDWSKALMIENNIPTAKYWSAKSKEEALEILKRFNQPLVIKADGLA
AGKGVTVCETIEESKEAIKDIFSGKFGSAGNKVILEEKIEGPEVSIFALCDGEKLIVLPPAQDHKRLLDGDNGPNTGGMG
AYAPALLINEQDLKDLTELVLIPTLKGLKKKNINYIGVIYAGLMLTSSGPKVIEFNCRFGDPECQALMPLMGEEFASVLF
ACARGEIENAPKLTFNSECSACIVAASKGYPESPQKGEKIAINVESNSSLQIFHAGTTIDKFDNIITSGGRVLSVVAQGE
SFDKAFDLAYSNLKKINFNGMHFREDIGYQVRNI
>Mature_434_residues
MKKNQKAEELKRILIVGSGGRENSIAWALSKNQSIEQIYVCPGNGGTAKFEKCICLKPNSEDEKIIINECKRLAIDLVII
GPEDPLAEGLANKMREAGLTVFGPGKDGAQLEASKDWSKALMIENNIPTAKYWSAKSKEEALEILKRFNQPLVIKADGLA
AGKGVTVCETIEESKEAIKDIFSGKFGSAGNKVILEEKIEGPEVSIFALCDGEKLIVLPPAQDHKRLLDGDNGPNTGGMG
AYAPALLINEQDLKDLTELVLIPTLKGLKKKNINYIGVIYAGLMLTSSGPKVIEFNCRFGDPECQALMPLMGEEFASVLF
ACARGEIENAPKLTFNSECSACIVAASKGYPESPQKGEKIAINVESNSSLQIFHAGTTIDKFDNIITSGGRVLSVVAQGE
SFDKAFDLAYSNLKKINFNGMHFREDIGYQVRNI

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=422, Percent_Identity=42.4170616113744, Blast_Score=347, Evalue=1e-95,
Organism=Homo sapiens, GI4503915, Length=422, Percent_Identity=42.4170616113744, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI209869995, Length=422, Percent_Identity=42.4170616113744, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI209869993, Length=422, Percent_Identity=42.4170616113744, Blast_Score=347, Evalue=2e-95,
Organism=Escherichia coli, GI1790438, Length=422, Percent_Identity=38.8625592417062, Blast_Score=322, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=40.3755868544601, Blast_Score=295, Evalue=3e-80,
Organism=Saccharomyces cerevisiae, GI6321203, Length=435, Percent_Identity=39.0804597701149, Blast_Score=312, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=43.287037037037, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI62471619, Length=424, Percent_Identity=43.8679245283019, Blast_Score=326, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 47096; Mature: 47096

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNQKAEELKRILIVGSGGRENSIAWALSKNQSIEQIYVCPGNGGTAKFEKCICLKPNS
CCCCCHHHHEEEEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCC
EDEKIIINECKRLAIDLVIIGPEDPLAEGLANKMREAGLTVFGPGKDGAQLEASKDWSKA
CCCEEHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCEEECCCCCCEE
LMIENNIPTAKYWSAKSKEEALEILKRFNQPLVIKADGLAAGKGVTVCETIEESKEAIKD
EEEECCCCCCHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
IFSGKFGSAGNKVILEEKIEGPEVSIFALCDGEKLIVLPPAQDHKRLLDGDNGPNTGGMG
HHCCCCCCCCCEEEEEECCCCCCEEEEEEECCCEEEEECCCHHHHHHHCCCCCCCCCCCC
AYAPALLINEQDLKDLTELVLIPTLKGLKKKNINYIGVIYAGLMLTSSGPKVIEFNCRFG
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEEECCC
DPECQALMPLMGEEFASVLFACARGEIENAPKLTFNSECSACIVAASKGYPESPQKGEKI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEECCCCCCCCCCCCEE
AINVESNSSLQIFHAGTTIDKFDNIITSGGRVLSVVAQGESFDKAFDLAYSNLKKINFNG
EEEECCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHEEECCCC
MHFREDIGYQVRNI
EEEHHHCCCCCCCC
>Mature Secondary Structure
MKKNQKAEELKRILIVGSGGRENSIAWALSKNQSIEQIYVCPGNGGTAKFEKCICLKPNS
CCCCCHHHHEEEEEEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCC
EDEKIIINECKRLAIDLVIIGPEDPLAEGLANKMREAGLTVFGPGKDGAQLEASKDWSKA
CCCEEHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCEEECCCCCCEE
LMIENNIPTAKYWSAKSKEEALEILKRFNQPLVIKADGLAAGKGVTVCETIEESKEAIKD
EEEECCCCCCHHCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHH
IFSGKFGSAGNKVILEEKIEGPEVSIFALCDGEKLIVLPPAQDHKRLLDGDNGPNTGGMG
HHCCCCCCCCCEEEEEECCCCCCEEEEEEECCCEEEEECCCHHHHHHHCCCCCCCCCCCC
AYAPALLINEQDLKDLTELVLIPTLKGLKKKNINYIGVIYAGLMLTSSGPKVIEFNCRFG
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEEECCC
DPECQALMPLMGEEFASVLFACARGEIENAPKLTFNSECSACIVAASKGYPESPQKGEKI
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEECCCCCCCCCCCCEE
AINVESNSSLQIFHAGTTIDKFDNIITSGGRVLSVVAQGESFDKAFDLAYSNLKKINFNG
EEEECCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHEEECCCC
MHFREDIGYQVRNI
EEEHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]