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The map label for this gene is glgX [H]

Identifier: 124026454

GI number: 124026454

Start: 1429812

End: 1431872

Strand: Direct

Name: glgX [H]

Synonym: NATL1_17491

Alternate gene names: 124026454

Gene position: 1429812-1431872 (Clockwise)

Preceding gene: 124026453

Following gene: 124026455

Centisome position: 76.68

GC content: 34.21

Gene sequence:

>2061_bases
TTGGGAAAAGTAAAAGTTGGTTCAGCATGGCCTTTGGGTAGCTCCGTTACTCTTGATGGGGTTAATTTCTCTATCGCAGC
ACCACATGCAACAAATTTAGAATTATTAATCTTCCAGAGTGCAGATGACGAGTATGCAAAAGAAACAATATCCTTAGATC
AGAAAAATAGGTCTGGAGATTATTGGCACGTTGAAATAGAGGGTCTAGCTGAAGGAACTTTATATGGATATAAAGTATCA
ATTGATGGGCAAAAAGCTGCCGAGGATCATATTGTTTTAGATCCATGTGCTAGAGCAATTACAGGGTGGGAGAATTACAT
AAGGAATCATAAAGAAAAAGTGAATAGAGGTTACGCTCATTGCCTCAAGGGAGTAGTAACTAAAAGAGATTATTTTGATT
TCAAATCTCACCCTAGGCCTAAACATTCATGGAATAAAACTATTATTTATGAATTACATGTTGGTGGATTTACGAAAAGC
AATGATTCAAGTATTAGTGAAAGTAAGAAAGGTACATTTTTAGGATTAATTGAAAAGATACCTTATCTTAAAAGTCTTGG
CATTACGACTATTGAATTGCTTCCGGTATTCGCTTTTGATTCTAATGATGCCCCTGAAGGACTTGTTAACTATTGGGGAT
ATAGTCCTATCAATTGGTTCACGCCACATCAAGATTTTGTAGATAGTTGTAATCCATTAGAAGCTAGGAGACAATTCAAA
AAGTTAGTAGAAGTTTGCCATGATAATGAGCTAGAAGTATTAATAGATGTTGTTTATAATCACACAACAGAAGGGAATCA
AAATGGTCCAAGTATAAGTTGGAAAAGTTTTGGTCCTAAAACTTACTATCATCAAGACGATAAGGAAAATTACCAAGATG
TGAGTGGATGTGGAAATAGTATTGCCGCTAATCGTCCTTTAGTTAGAAAATTAATATTGGAATCATTGCGTTGTTGGGCA
ATTGAGTTAGGTGTTGATGGATTTCGTTTTGACTTAGGTATTGCTTTGAGTAGAGGCGAAAATTTAATTCCTTTAGATAA
ACCTCCCTTATTTGAAGAAATAGAAGCAGATCCTAAACTAAGCGATGTGAAGTTAATCAGTGAGCCTTGGGATTGTGGTG
GACTTTACAAACTAGGTGATTTTCCAGCGAAACAATTCAGAACTTGGAATGGTCATTTTAGAGATGATATTAGACGATTT
TGGAAAGGAGATAAAGGCACAATCTGGCCACTTAAAGATAGATTAATAGGTAATAAGTCCCTATATAATGATAATAATTT
AGCAAATATATTTAGTATTAATTTCATAACTTCTCATGATGGATTCACATTAAAAGATCTAGTTAGTTTTAACAAAAAGC
ATAACCTTGCAAATGGGGAAAATAATAGAGATGGGGAGAACAATAATAATAGTTATAATTATGGAGTTGAAGGACCAACT
ACTGATAAAGAAATAAATAATTTAAGGCAAAAACAACAAAGAAATCTTCTTGCAACACTTCTTTTATCTCCTGGAATACC
AATGATTTTAATGGGAGACGAATTAGGTAGAAGCCAAGGAGGAAATAACAACACATGGTGCCAAGACAACCCACTTGGTT
GGATGGATTGGAATCACAACAATTGTGATCATGACTTAAAAGAATTTGTATTAAAGCTGATATCTCTAAGGAAACAACTA
TCTGAATTTTTCAGTCCTGATATTATTTATGATTGTCAAAAAGATAATACCAAGGTCAAAACATCTCATTTTTGGATTCA
GTGGCATGGCATTAAAGTTAATAAACCTGACTGGGGTGACTGGTCACATACTCTTGGATTTAGCATTAACAAAAACAATG
ATGGCTCAGCAATCTGGCTTGGCTTCAATGCTTACAAAGAATCAATGCTTTTTGACTTACCAACCCCAATTTCTCCTTGG
AAAAAATACATTGATACGTCTATCTTGAAAACTAAAAATGTCTCCAAAAAACCCCTAGCGAATCAATCAAATGTTCGGAT
TGAAAGTAATAGTCTTGTTCTTATGGTCTCCAGCGAGTATTCAAAAAAAATTAAGCTATGA

Upstream 100 bases:

>100_bases
TTTTATGATTGAATTTCGTTTTTTATAACTTGTTAAAACAAACTAACCACCTAACTTGTAGGGAAAGTTACTTGATTATT
TTTGTAAAGGACAAACACAA

Downstream 100 bases:

>100_bases
GAATCAAAATTAAGCGGGCGGCGGGAATCGAACCCGCATCATCAGCTTGGAAGGCTGAGGTTTTACCACTAAACTACGCC
CGCGCTAGAAATAAAAACTA

Product: putative isoamylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 685

Protein sequence:

>686_residues
MGKVKVGSAWPLGSSVTLDGVNFSIAAPHATNLELLIFQSADDEYAKETISLDQKNRSGDYWHVEIEGLAEGTLYGYKVS
IDGQKAAEDHIVLDPCARAITGWENYIRNHKEKVNRGYAHCLKGVVTKRDYFDFKSHPRPKHSWNKTIIYELHVGGFTKS
NDSSISESKKGTFLGLIEKIPYLKSLGITTIELLPVFAFDSNDAPEGLVNYWGYSPINWFTPHQDFVDSCNPLEARRQFK
KLVEVCHDNELEVLIDVVYNHTTEGNQNGPSISWKSFGPKTYYHQDDKENYQDVSGCGNSIAANRPLVRKLILESLRCWA
IELGVDGFRFDLGIALSRGENLIPLDKPPLFEEIEADPKLSDVKLISEPWDCGGLYKLGDFPAKQFRTWNGHFRDDIRRF
WKGDKGTIWPLKDRLIGNKSLYNDNNLANIFSINFITSHDGFTLKDLVSFNKKHNLANGENNRDGENNNNSYNYGVEGPT
TDKEINNLRQKQQRNLLATLLLSPGIPMILMGDELGRSQGGNNNTWCQDNPLGWMDWNHNNCDHDLKEFVLKLISLRKQL
SEFFSPDIIYDCQKDNTKVKTSHFWIQWHGIKVNKPDWGDWSHTLGFSINKNNDGSAIWLGFNAYKESMLFDLPTPISPW
KKYIDTSILKTKNVSKKPLANQSNVRIESNSLVLMVSSEYSKKIKL

Sequences:

>Translated_686_residues
MGKVKVGSAWPLGSSVTLDGVNFSIAAPHATNLELLIFQSADDEYAKETISLDQKNRSGDYWHVEIEGLAEGTLYGYKVS
IDGQKAAEDHIVLDPCARAITGWENYIRNHKEKVNRGYAHCLKGVVTKRDYFDFKSHPRPKHSWNKTIIYELHVGGFTKS
NDSSISESKKGTFLGLIEKIPYLKSLGITTIELLPVFAFDSNDAPEGLVNYWGYSPINWFTPHQDFVDSCNPLEARRQFK
KLVEVCHDNELEVLIDVVYNHTTEGNQNGPSISWKSFGPKTYYHQDDKENYQDVSGCGNSIAANRPLVRKLILESLRCWA
IELGVDGFRFDLGIALSRGENLIPLDKPPLFEEIEADPKLSDVKLISEPWDCGGLYKLGDFPAKQFRTWNGHFRDDIRRF
WKGDKGTIWPLKDRLIGNKSLYNDNNLANIFSINFITSHDGFTLKDLVSFNKKHNLANGENNRDGENNNNSYNYGVEGPT
TDKEINNLRQKQQRNLLATLLLSPGIPMILMGDELGRSQGGNNNTWCQDNPLGWMDWNHNNCDHDLKEFVLKLISLRKQL
SEFFSPDIIYDCQKDNTKVKTSHFWIQWHGIKVNKPDWGDWSHTLGFSINKNNDGSAIWLGFNAYKESMLFDLPTPISPW
KKYIDTSILKTKNVSKKPLANQSNVRIESNSLVLMVSSEYSKKIKL
>Mature_685_residues
GKVKVGSAWPLGSSVTLDGVNFSIAAPHATNLELLIFQSADDEYAKETISLDQKNRSGDYWHVEIEGLAEGTLYGYKVSI
DGQKAAEDHIVLDPCARAITGWENYIRNHKEKVNRGYAHCLKGVVTKRDYFDFKSHPRPKHSWNKTIIYELHVGGFTKSN
DSSISESKKGTFLGLIEKIPYLKSLGITTIELLPVFAFDSNDAPEGLVNYWGYSPINWFTPHQDFVDSCNPLEARRQFKK
LVEVCHDNELEVLIDVVYNHTTEGNQNGPSISWKSFGPKTYYHQDDKENYQDVSGCGNSIAANRPLVRKLILESLRCWAI
ELGVDGFRFDLGIALSRGENLIPLDKPPLFEEIEADPKLSDVKLISEPWDCGGLYKLGDFPAKQFRTWNGHFRDDIRRFW
KGDKGTIWPLKDRLIGNKSLYNDNNLANIFSINFITSHDGFTLKDLVSFNKKHNLANGENNRDGENNNNSYNYGVEGPTT
DKEINNLRQKQQRNLLATLLLSPGIPMILMGDELGRSQGGNNNTWCQDNPLGWMDWNHNNCDHDLKEFVLKLISLRKQLS
EFFSPDIIYDCQKDNTKVKTSHFWIQWHGIKVNKPDWGDWSHTLGFSINKNNDGSAIWLGFNAYKESMLFDLPTPISPWK
KYIDTSILKTKNVSKKPLANQSNVRIESNSLVLMVSSEYSKKIKL

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=574, Percent_Identity=38.5017421602787, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 78086; Mature: 77954

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVKVGSAWPLGSSVTLDGVNFSIAAPHATNLELLIFQSADDEYAKETISLDQKNRSGD
CCCEECCCCCCCCCCEEECCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCC
YWHVEIEGLAEGTLYGYKVSIDGQKAAEDHIVLDPCARAITGWENYIRNHKEKVNRGYAH
EEEEEEEEEECCEEEEEEEEECCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
CLKGVVTKRDYFDFKSHPRPKHSWNKTIIYELHVGGFTKSNDSSISESKKGTFLGLIEKI
HHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHCCCCHHHHHHHHC
PYLKSLGITTIELLPVFAFDSNDAPEGLVNYWGYSPINWFTPHQDFVDSCNPLEARRQFK
CHHHHCCCEEEEEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHHH
KLVEVCHDNELEVLIDVVYNHTTEGNQNGPSISWKSFGPKTYYHQDDKENYQDVSGCGNS
HHHHHHCCCCHHEEEEEHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHCCCCC
IAANRPLVRKLILESLRCWAIELGVDGFRFDLGIALSRGENLIPLDKPPLFEEIEADPKL
CCCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCHHHHCCCCCC
SDVKLISEPWDCGGLYKLGDFPAKQFRTWNGHFRDDIRRFWKGDKGTIWPLKDRLIGNKS
CCEEEECCCCCCCCEEECCCCCHHHHCCCCCCHHHHHHHHHCCCCCCEEEHHHHHCCCCC
LYNDNNLANIFSINFITSHDGFTLKDLVSFNKKHNLANGENNRDGENNNNSYNYGVEGPT
CCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCC
TDKEINNLRQKQQRNLLATLLLSPGIPMILMGDELGRSQGGNNNTWCQDNPLGWMDWNHN
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHCCCCCCCCCCCCCCCCCEEECCCCC
NCDHDLKEFVLKLISLRKQLSEFFSPDIIYDCQKDNTKVKTSHFWIQWHGIKVNKPDWGD
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEEEEEEEECCCCCCC
WSHTLGFSINKNNDGSAIWLGFNAYKESMLFDLPTPISPWKKYIDTSILKTKNVSKKPLA
CCCEECEEEECCCCCCEEEEEHHHHHHCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCC
NQSNVRIESNSLVLMVSSEYSKKIKL
CCCCEEEECCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
GKVKVGSAWPLGSSVTLDGVNFSIAAPHATNLELLIFQSADDEYAKETISLDQKNRSGD
CCEECCCCCCCCCCEEECCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCC
YWHVEIEGLAEGTLYGYKVSIDGQKAAEDHIVLDPCARAITGWENYIRNHKEKVNRGYAH
EEEEEEEEEECCEEEEEEEEECCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
CLKGVVTKRDYFDFKSHPRPKHSWNKTIIYELHVGGFTKSNDSSISESKKGTFLGLIEKI
HHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHCCCCHHHHHHHHC
PYLKSLGITTIELLPVFAFDSNDAPEGLVNYWGYSPINWFTPHQDFVDSCNPLEARRQFK
CHHHHCCCEEEEEEEEEEECCCCCCCHHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHHH
KLVEVCHDNELEVLIDVVYNHTTEGNQNGPSISWKSFGPKTYYHQDDKENYQDVSGCGNS
HHHHHHCCCCHHEEEEEHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHCCCCC
IAANRPLVRKLILESLRCWAIELGVDGFRFDLGIALSRGENLIPLDKPPLFEEIEADPKL
CCCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCHHHHCCCCCC
SDVKLISEPWDCGGLYKLGDFPAKQFRTWNGHFRDDIRRFWKGDKGTIWPLKDRLIGNKS
CCEEEECCCCCCCCEEECCCCCHHHHCCCCCCHHHHHHHHHCCCCCCEEEHHHHHCCCCC
LYNDNNLANIFSINFITSHDGFTLKDLVSFNKKHNLANGENNRDGENNNNSYNYGVEGPT
CCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCC
TDKEINNLRQKQQRNLLATLLLSPGIPMILMGDELGRSQGGNNNTWCQDNPLGWMDWNHN
CHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHCCCCCCCCCCCCCCCCCEEECCCCC
NCDHDLKEFVLKLISLRKQLSEFFSPDIIYDCQKDNTKVKTSHFWIQWHGIKVNKPDWGD
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEEEEEEEECCCCCCC
WSHTLGFSINKNNDGSAIWLGFNAYKESMLFDLPTPISPWKKYIDTSILKTKNVSKKPLA
CCCEECEEEECCCCCCEEEEEHHHHHHCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCC
NQSNVRIESNSLVLMVSSEYSKKIKL
CCCCEEEECCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]