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The map label for this gene is cbiB [H]

Identifier: 124026442

GI number: 124026442

Start: 1422604

End: 1423548

Strand: Direct

Name: cbiB [H]

Synonym: NATL1_17371

Alternate gene names: 124026442

Gene position: 1422604-1423548 (Clockwise)

Preceding gene: 124026441

Following gene: 124026446

Centisome position: 76.29

GC content: 32.17

Gene sequence:

>945_bases
TTGCCACACCCTGTCCAATTGATGGGTTTGGTTATTAGTTTTTCAAGAGGGTTAACAGAGAGAATTGCGAAAGATAATAA
ACTAAAGTTATATTTAGGAGGATTAATAATTACAATTATAGTTGTATCATTAAGTGGAATATCTGGTTGGATTATAGAAA
GATTATTTTTATTCTTTGAAATAAAAAATCCTATTATAAGTACATTATTTTTTGGTTTGATTTTAAGCAGCTCTCTAGCC
TCAAGAAGTCTAAATAAAAGTATTCTTGAAATTTTAAATATAATAAGCAAAGAAAATACTATAGACAATTTAAGTAATAT
AAGACAAAAATTAAGTCTTATAGTTGGTAGAGATGTTGAAAATTTGAATAGAAATGAAGTTCTTAGAGCTTTAGCTGAAA
CAGCAAGTGAAAATTCTGTGGATGGTATCTTTGCTCCATTATTTTGGATGTTTTTAGGAACTATTTTATGGGAATTCAAT
CAATCCTTGCCAGGCCCATTGGCGATGGCATGGATTTTCAAAGCATCGAGCACCATTGATTCAATGCTTGGATACAAAGA
GGGGAAATTAAAGTGGCTTGGCTTTACTGGTGCGAAGCTGGATGATCTGATGGTATGGATACCCTCAAGAATTGTATTAA
TTACTCTTCCTTTATGCTGCAAAACAAAGCAGTCAATTTTCAAAACAATAAAAATTGCTTGGAAGGATGGAATTGCTGAC
TCTTCGCCAAACTCAGGAATTTCTGAAGCAATTTTTGCTTATTGTGCCGAAGTAAAAATGGGGGGGGTAAACTATTACAA
AGGGCGAAAAAGTATAAAGCCAATAATTGCAGAACCATACCCTATAGCAAGTATTAATTCAGTCAGAAAAATACTTGGTC
TGAGTCTTAGGCTTGAGCTAGTTTGGCTTATAGGTTTTTACTTGATAATAAAATTTATAAATTAA

Upstream 100 bases:

>100_bases
CACTTCGCTCGATGTTTAGTTGGCTTAAATCAGACTAAATATCATTCACTTAATCTTAGTTGCTGTTATTCTTGACCTTT
TAATAGGTGATCCAAAAAGC

Downstream 100 bases:

>100_bases
TAAATTCAAAAAGCACCTCTATCCATTGGAAAGATTAAAACGCCTAACGTAAGAATAATGATTTCCAAATCAAGTAGAAA
ATTCCTATTTCTTGCATAAA

Product: putative cobalamin biosynthetic protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MPHPVQLMGLVISFSRGLTERIAKDNKLKLYLGGLIITIIVVSLSGISGWIIERLFLFFEIKNPIISTLFFGLILSSSLA
SRSLNKSILEILNIISKENTIDNLSNIRQKLSLIVGRDVENLNRNEVLRALAETASENSVDGIFAPLFWMFLGTILWEFN
QSLPGPLAMAWIFKASSTIDSMLGYKEGKLKWLGFTGAKLDDLMVWIPSRIVLITLPLCCKTKQSIFKTIKIAWKDGIAD
SSPNSGISEAIFAYCAEVKMGGVNYYKGRKSIKPIIAEPYPIASINSVRKILGLSLRLELVWLIGFYLIIKFIN

Sequences:

>Translated_314_residues
MPHPVQLMGLVISFSRGLTERIAKDNKLKLYLGGLIITIIVVSLSGISGWIIERLFLFFEIKNPIISTLFFGLILSSSLA
SRSLNKSILEILNIISKENTIDNLSNIRQKLSLIVGRDVENLNRNEVLRALAETASENSVDGIFAPLFWMFLGTILWEFN
QSLPGPLAMAWIFKASSTIDSMLGYKEGKLKWLGFTGAKLDDLMVWIPSRIVLITLPLCCKTKQSIFKTIKIAWKDGIAD
SSPNSGISEAIFAYCAEVKMGGVNYYKGRKSIKPIIAEPYPIASINSVRKILGLSLRLELVWLIGFYLIIKFIN
>Mature_313_residues
PHPVQLMGLVISFSRGLTERIAKDNKLKLYLGGLIITIIVVSLSGISGWIIERLFLFFEIKNPIISTLFFGLILSSSLAS
RSLNKSILEILNIISKENTIDNLSNIRQKLSLIVGRDVENLNRNEVLRALAETASENSVDGIFAPLFWMFLGTILWEFNQ
SLPGPLAMAWIFKASSTIDSMLGYKEGKLKWLGFTGAKLDDLMVWIPSRIVLITLPLCCKTKQSIFKTIKIAWKDGIADS
SPNSGISEAIFAYCAEVKMGGVNYYKGRKSIKPIIAEPYPIASINSVRKILGLSLRLELVWLIGFYLIIKFIN

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 35063; Mature: 34932

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHPVQLMGLVISFSRGLTERIAKDNKLKLYLGGLIITIIVVSLSGISGWIIERLFLFFE
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IKNPIISTLFFGLILSSSLASRSLNKSILEILNIISKENTIDNLSNIRQKLSLIVGRDVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
NLNRNEVLRALAETASENSVDGIFAPLFWMFLGTILWEFNQSLPGPLAMAWIFKASSTID
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
SMLGYKEGKLKWLGFTGAKLDDLMVWIPSRIVLITLPLCCKTKQSIFKTIKIAWKDGIAD
HHHCCCCCCEEEEECCCCCHHHHHHHHCCEEEEEEECHHHCCHHHHHHHHHHHHHCCCCC
SSPNSGISEAIFAYCAEVKMGGVNYYKGRKSIKPIIAEPYPIASINSVRKILGLSLRLEL
CCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VWLIGFYLIIKFIN
HHHHHHHHHHHHCC
>Mature Secondary Structure 
PHPVQLMGLVISFSRGLTERIAKDNKLKLYLGGLIITIIVVSLSGISGWIIERLFLFFE
CCHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IKNPIISTLFFGLILSSSLASRSLNKSILEILNIISKENTIDNLSNIRQKLSLIVGRDVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
NLNRNEVLRALAETASENSVDGIFAPLFWMFLGTILWEFNQSLPGPLAMAWIFKASSTID
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
SMLGYKEGKLKWLGFTGAKLDDLMVWIPSRIVLITLPLCCKTKQSIFKTIKIAWKDGIAD
HHHCCCCCCEEEEECCCCCHHHHHHHHCCEEEEEEECHHHCCHHHHHHHHHHHHHCCCCC
SSPNSGISEAIFAYCAEVKMGGVNYYKGRKSIKPIIAEPYPIASINSVRKILGLSLRLEL
CCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VWLIGFYLIIKFIN
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]