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The map label for this gene is cysS

Identifier: 124026295

GI number: 124026295

Start: 1298261

End: 1299763

Strand: Direct

Name: cysS

Synonym: NATL1_15891

Alternate gene names: 124026295

Gene position: 1298261-1299763 (Clockwise)

Preceding gene: 124026294

Following gene: 124026297

Centisome position: 69.62

GC content: 31.2

Gene sequence:

>1503_bases
TTGTCCCTAAAATTCACAAACACCTTATCCAAAAAGAAAGAGGATTTTATTTCTATAAATCCTAATCAAGTTAAAATATA
TTGTTGTGGTGTAACTGTTTATGATCTTTGTCATCTTGGTCATGCAAGAAGTTACCTGAATTGGGATGTATTGAGACGGT
TTTTAATTTGGAAAGGATTTGAAGTTAAATTTGTACAAAACTTCACTGATATTGATGACAAAATTATTAATCGAGCAAAT
AAAGAAGGATGTTCTACTGATGAATTAAGTGAAAGAAATATTGATGAATTCCACAAAGATATGGATACTCTTTCCATTCT
TAGACCAACTAGCATGCCAAGAGCAACAAAATGCCTCCATCAAATTATTAATTTCATAGAAGAATTAGAACAAAAAAAAG
TAGCTTATTCATCAAATGGTGATGTTTATTTTTCAGTAAATAAACATAAAAATTATGGGAAACTTAGTGGAAGAGAAATT
GAGAATCAGATAGATAATGCAGCAGGTAGATTAAAAACAAATCAAAAAGAAAGTAAAAAGAACTCGCTTGATTTTGCTCT
CTGGAAAAAGTCCAACTCAGGTGAGGTTAGTTACTCTTCGCCTTGGGGAAACGGCAGGCCAGGTTGGCATATTGAGTGTT
CAGCAATGGTTAAACAAGAATTAGGAGAAAGTATTGATATTCACCTTGGTGGATCAGACCTAATATTTCCTCATCATGAG
AATGAAATAGCTCAATCTGAAGCCTGTAATGGGAAAGAGTTAGCAAAATACTGGCTTCACAATGGAATGGTTAATGTGGG
AGGTGAAAAGATGAGTAAATCACTAGGGAATTTTACAACCATTAGGTCTTTATTAGAGGAAGGTATTTCACCTATGACTT
TGAGATTTTTTGTATTACAAACTAATTACCGAAAGCCCCTAGATTTTACTCAAGAAGCACTAAAAGCTGCTTCAAAAGGG
TGGGAAAGATTAAATAATTGCCTGTCTTTTGGTTATATTTATAAAATTAAAGATCAAGCTAAAAACGAAATCATCCTAGA
TAAACCGATAAAAAAATCTGCCAACACTAATCTTGATAAAGAATCATTTAAATTATTATCAGACTTTGAAAGGTATATGG
ATGACGACCTAAATACATCTGGAGCTTTATCTATTCTTTTTGAACTATCTCAACCAATTAGAAAGATCATTAACTTCTTA
AAAGAAAAAGATATTAATGAAGTTGATCAAGATGAATTAAATCAAGTTTTTTATAAATGGGAACTACTCTCTGAGTTAGC
AGCAGTTCTAGGTTTAAAAGTAAATTTAAATCATGAAAACCCAAAAAACAATCCTGAACTTGACACTAATAAGATTGAAG
AACTTATCAAGAAGAGATCCTTAGCAAAAGCTAATAAAGACTTTTTACTCGCTGATAAGATAAGAGATGATTTTAAAAAT
ATTGGAATTGATTTAATTGATAAACCTAAAGGGGTTACTGAATGGAAACAACTTTCAGATTAA

Upstream 100 bases:

>100_bases
AGTGTCCCTCTTATCGTTGAAACTGGCGTTGGAGTAAATTGGATGGAGGCAAAATAGTTGCTGACAAATTCAATAATTTC
TCACATTTTCATCTAAGAAA

Downstream 100 bases:

>100_bases
GCAAATGTTTCTATTGTAGATAAGACCGTTAAATAAAGTCCAATAGCAATAACTGGTGGAGACACCCAGCGCAGCATAAA
CTTTAGATACCTTCTAACTC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSLKFTNTLSKKKEDFISINPNQVKIYCCGVTVYDLCHLGHARSYLNWDVLRRFLIWKGFEVKFVQNFTDIDDKIINRAN
KEGCSTDELSERNIDEFHKDMDTLSILRPTSMPRATKCLHQIINFIEELEQKKVAYSSNGDVYFSVNKHKNYGKLSGREI
ENQIDNAAGRLKTNQKESKKNSLDFALWKKSNSGEVSYSSPWGNGRPGWHIECSAMVKQELGESIDIHLGGSDLIFPHHE
NEIAQSEACNGKELAKYWLHNGMVNVGGEKMSKSLGNFTTIRSLLEEGISPMTLRFFVLQTNYRKPLDFTQEALKAASKG
WERLNNCLSFGYIYKIKDQAKNEIILDKPIKKSANTNLDKESFKLLSDFERYMDDDLNTSGALSILFELSQPIRKIINFL
KEKDINEVDQDELNQVFYKWELLSELAAVLGLKVNLNHENPKNNPELDTNKIEELIKKRSLAKANKDFLLADKIRDDFKN
IGIDLIDKPKGVTEWKQLSD

Sequences:

>Translated_500_residues
MSLKFTNTLSKKKEDFISINPNQVKIYCCGVTVYDLCHLGHARSYLNWDVLRRFLIWKGFEVKFVQNFTDIDDKIINRAN
KEGCSTDELSERNIDEFHKDMDTLSILRPTSMPRATKCLHQIINFIEELEQKKVAYSSNGDVYFSVNKHKNYGKLSGREI
ENQIDNAAGRLKTNQKESKKNSLDFALWKKSNSGEVSYSSPWGNGRPGWHIECSAMVKQELGESIDIHLGGSDLIFPHHE
NEIAQSEACNGKELAKYWLHNGMVNVGGEKMSKSLGNFTTIRSLLEEGISPMTLRFFVLQTNYRKPLDFTQEALKAASKG
WERLNNCLSFGYIYKIKDQAKNEIILDKPIKKSANTNLDKESFKLLSDFERYMDDDLNTSGALSILFELSQPIRKIINFL
KEKDINEVDQDELNQVFYKWELLSELAAVLGLKVNLNHENPKNNPELDTNKIEELIKKRSLAKANKDFLLADKIRDDFKN
IGIDLIDKPKGVTEWKQLSD
>Mature_499_residues
SLKFTNTLSKKKEDFISINPNQVKIYCCGVTVYDLCHLGHARSYLNWDVLRRFLIWKGFEVKFVQNFTDIDDKIINRANK
EGCSTDELSERNIDEFHKDMDTLSILRPTSMPRATKCLHQIINFIEELEQKKVAYSSNGDVYFSVNKHKNYGKLSGREIE
NQIDNAAGRLKTNQKESKKNSLDFALWKKSNSGEVSYSSPWGNGRPGWHIECSAMVKQELGESIDIHLGGSDLIFPHHEN
EIAQSEACNGKELAKYWLHNGMVNVGGEKMSKSLGNFTTIRSLLEEGISPMTLRFFVLQTNYRKPLDFTQEALKAASKGW
ERLNNCLSFGYIYKIKDQAKNEIILDKPIKKSANTNLDKESFKLLSDFERYMDDDLNTSGALSILFELSQPIRKIINFLK
EKDINEVDQDELNQVFYKWELLSELAAVLGLKVNLNHENPKNNPELDTNKIEELIKKRSLAKANKDFLLADKIRDDFKNI
GIDLIDKPKGVTEWKQLSD

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=319, Percent_Identity=42.0062695924765, Blast_Score=246, Evalue=5e-65,
Organism=Homo sapiens, GI21269877, Length=233, Percent_Identity=42.0600858369099, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI10835051, Length=233, Percent_Identity=42.0600858369099, Blast_Score=182, Evalue=8e-46,
Organism=Homo sapiens, GI62240992, Length=233, Percent_Identity=42.0600858369099, Blast_Score=182, Evalue=9e-46,
Organism=Homo sapiens, GI303304987, Length=233, Percent_Identity=42.0600858369099, Blast_Score=182, Evalue=9e-46,
Organism=Escherichia coli, GI1786737, Length=499, Percent_Identity=41.6833667334669, Blast_Score=350, Evalue=9e-98,
Organism=Caenorhabditis elegans, GI17509795, Length=299, Percent_Identity=35.1170568561873, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17509797, Length=299, Percent_Identity=35.1170568561873, Blast_Score=160, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324082, Length=306, Percent_Identity=37.2549019607843, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI19922218, Length=397, Percent_Identity=36.272040302267, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI19922364, Length=316, Percent_Identity=37.6582278481013, Blast_Score=189, Evalue=6e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_PROM1 (A2C3T6)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015411.1
- ProteinModelPortal:   A2C3T6
- STRING:   A2C3T6
- GeneID:   4779352
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_15891
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   PMAR167555:NATL1_15891-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 57429; Mature: 57298

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKFTNTLSKKKEDFISINPNQVKIYCCGVTVYDLCHLGHARSYLNWDVLRRFLIWKGF
CCCCCCCHHHHCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
EVKFVQNFTDIDDKIINRANKEGCSTDELSERNIDEFHKDMDTLSILRPTSMPRATKCLH
EEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
QIINFIEELEQKKVAYSSNGDVYFSVNKHKNYGKLSGREIENQIDNAAGRLKTNQKESKK
HHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
NSLDFALWKKSNSGEVSYSSPWGNGRPGWHIECSAMVKQELGESIDIHLGGSDLIFPHHE
CCCCEEEEECCCCCCEEECCCCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCEECCCCC
NEIAQSEACNGKELAKYWLHNGMVNVGGEKMSKSLGNFTTIRSLLEEGISPMTLRFFVLQ
CHHHHHHCCCHHHHHHHHHHCCCEECCHHHHHHHHCHHHHHHHHHHHCCCCEEEEEEEEE
TNYRKPLDFTQEALKAASKGWERLNNCLSFGYIYKIKDQAKNEIILDKPIKKSANTNLDK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEECCCCCCCCCCCCCH
ESFKLLSDFERYMDDDLNTSGALSILFELSQPIRKIINFLKEKDINEVDQDELNQVFYKW
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
ELLSELAAVLGLKVNLNHENPKNNPELDTNKIEELIKKRSLAKANKDFLLADKIRDDFKN
HHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHH
IGIDLIDKPKGVTEWKQLSD
CCCEEECCCCCCHHHHHCCC
>Mature Secondary Structure 
SLKFTNTLSKKKEDFISINPNQVKIYCCGVTVYDLCHLGHARSYLNWDVLRRFLIWKGF
CCCCCCHHHHCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
EVKFVQNFTDIDDKIINRANKEGCSTDELSERNIDEFHKDMDTLSILRPTSMPRATKCLH
EEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
QIINFIEELEQKKVAYSSNGDVYFSVNKHKNYGKLSGREIENQIDNAAGRLKTNQKESKK
HHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
NSLDFALWKKSNSGEVSYSSPWGNGRPGWHIECSAMVKQELGESIDIHLGGSDLIFPHHE
CCCCEEEEECCCCCCEEECCCCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCEECCCCC
NEIAQSEACNGKELAKYWLHNGMVNVGGEKMSKSLGNFTTIRSLLEEGISPMTLRFFVLQ
CHHHHHHCCCHHHHHHHHHHCCCEECCHHHHHHHHCHHHHHHHHHHHCCCCEEEEEEEEE
TNYRKPLDFTQEALKAASKGWERLNNCLSFGYIYKIKDQAKNEIILDKPIKKSANTNLDK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEECCCCCCCCCCCCCH
ESFKLLSDFERYMDDDLNTSGALSILFELSQPIRKIINFLKEKDINEVDQDELNQVFYKW
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
ELLSELAAVLGLKVNLNHENPKNNPELDTNKIEELIKKRSLAKANKDFLLADKIRDDFKN
HHHHHHHHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHH
IGIDLIDKPKGVTEWKQLSD
CCCEEECCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA