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The map label for this gene is leuA

Identifier: 124026226

GI number: 124026226

Start: 1233783

End: 1235402

Strand: Reverse

Name: leuA

Synonym: NATL1_15201

Alternate gene names: 124026226

Gene position: 1235402-1233783 (Counterclockwise)

Preceding gene: 124026228

Following gene: 124026225

Centisome position: 66.25

GC content: 39.44

Gene sequence:

>1620_bases
ATGGCCAAAGATCCGGGCCGAGTTTTAATTTTTGACACTACATTAAGAGATGGAGAGCAATCTCCTGGAGCTAGTCTTAA
TTTAGAAGAAAAGTTAGCTATTGCTCAACAATTAGCAAGATTAGGAGTTGATGTTATTGAGGCAGGATTCCCTTTTGCTA
GCCCTGGGGATTTCGCTGCAGTTCAGAAAATAGCTGAGAATGTAGGAGGAGAAGAAGGACCTATCATTTGCGGACTATCA
AGAGCCTCAAAACCTGATATCAAAGCTTGTGCCAATGCGATTGCTCCAGCCCCAAAAAAAAGGATTCATACCTTCATTGC
AACAAGTGATATACATCTTGAACATAAATTAAGGAAATCCAGAAAAGAAGTACTTGATATCGTTCCAGATATGGTTGGCT
ATGCTAAAAGTTTTGTTGATGACGTTGAATTTTCCTGTGAAGATGCAGCAAGAAGTGATTTAGATTTTCTTTATGAAGTA
ATAGAACTAGCCATATCCTCAGGGGCTAATACAATAAATATTCCAGATACAGTTGGTTATATAACCCCTTCTGAATTTGG
AGATTTGATATTAAATATCAACGAAAATGTTCCAAATATCAATGAGGCAGTTCTGTCAGTTCATGGTCACAACGATTTAG
GACTTGCTGTCGCAAACTTCCTTGAAGCTGTAAAGAATGGAGCTAGACAACTTGAATGCACCATTAACGGAATAGGTGAG
AGAGCAGGTAATGCTGCTTTAGAAGAATTAATCATGGCGCTTCATGTAAGAAGATCATATTTTAATCCATTTTTTGGGAG
GCCTCCTGAATCCCCTACTCCTTTGACAGCAGTTAGAACAGAGGAGATAACTAAGTCTTCTCGCTTGGTTTCAAATTTGA
CTGGGATGGTCGTACAACCGAACAAAGCAATTGTTGGGGCAAACGCTTTTGCGCATGAATCTGGAATACACCAAGATGGA
GTATTGAAAAATAGGCTTACATATGAAATTATCGATGCAAAAACAGTAGGGTTGTCTGACAATAAGATTTCTTTGGGAAA
ATTAAGTGGTAGGAGTGCTGTTCGAGCAAGATTAGAGGACCTTGGATATGATTTAAACAGAGAAGATCTTAATGACGCTT
TCGCTAGATTTAAAGATTTAGCCGATAGAAAAAGAGAGATAACAGATCGTGATCTAGAGGCCATTGTTAGTGAACAAGTT
CAGCTGCCAGAAGCATTGTTCCAATTAAAATTGGTCCAAGTAAGCTGTGGCACTTCTCTAATGCCAACTGCAACAGTAAC
TGTTGTTGGAGAAGATGGAGAGGAGAAGACCGCCGTCTCTCTTGGAACAGGTCCTGTTGATGCAGTAGTACGAGCCTTGG
ATTCCCTAACTGAAGAACCTAATGAATTGATTGAATTCTCAGTAAAGTCAGTTACAGAGGGGATAGATGCTCTGGGTGAA
GTTACTATTAGAATAAGAAGAGATGGAAATCTCTTTTCTGGCCATTCTGCAGATACTGACGTTGTTGTTGCCGCTGCTCA
AGCATACATAAATGCTCTTAATAGATTAGTAGCTGCTCATGGAAGGAAATCCATTCATCCACAACATGATTTGGCTAAGG
TAGACAAAAAAGGGATTTGA

Upstream 100 bases:

>100_bases
AATCTAATAGTCTTTGTAGATAATTAAAATAAATATACTACAATTAACTCAATTCATATAGATCTTAAACTGGTCTAATT
ATCTTTATATATTTCATGCT

Downstream 100 bases:

>100_bases
TAACCTAGTTTTCATTAGCTATCTTTAAAAGATTTCCGTAAATTTTTTGTAGTTAAATTGCACTAATGTATGAATCAATG
ATTAATATGTGATGTGACAA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MAKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASPGDFAAVQKIAENVGGEEGPIICGLS
RASKPDIKACANAIAPAPKKRIHTFIATSDIHLEHKLRKSRKEVLDIVPDMVGYAKSFVDDVEFSCEDAARSDLDFLYEV
IELAISSGANTINIPDTVGYITPSEFGDLILNINENVPNINEAVLSVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNAALEELIMALHVRRSYFNPFFGRPPESPTPLTAVRTEEITKSSRLVSNLTGMVVQPNKAIVGANAFAHESGIHQDG
VLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEDLGYDLNREDLNDAFARFKDLADRKREITDRDLEAIVSEQV
QLPEALFQLKLVQVSCGTSLMPTATVTVVGEDGEEKTAVSLGTGPVDAVVRALDSLTEEPNELIEFSVKSVTEGIDALGE
VTIRIRRDGNLFSGHSADTDVVVAAAQAYINALNRLVAAHGRKSIHPQHDLAKVDKKGI

Sequences:

>Translated_539_residues
MAKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASPGDFAAVQKIAENVGGEEGPIICGLS
RASKPDIKACANAIAPAPKKRIHTFIATSDIHLEHKLRKSRKEVLDIVPDMVGYAKSFVDDVEFSCEDAARSDLDFLYEV
IELAISSGANTINIPDTVGYITPSEFGDLILNINENVPNINEAVLSVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
RAGNAALEELIMALHVRRSYFNPFFGRPPESPTPLTAVRTEEITKSSRLVSNLTGMVVQPNKAIVGANAFAHESGIHQDG
VLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEDLGYDLNREDLNDAFARFKDLADRKREITDRDLEAIVSEQV
QLPEALFQLKLVQVSCGTSLMPTATVTVVGEDGEEKTAVSLGTGPVDAVVRALDSLTEEPNELIEFSVKSVTEGIDALGE
VTIRIRRDGNLFSGHSADTDVVVAAAQAYINALNRLVAAHGRKSIHPQHDLAKVDKKGI
>Mature_538_residues
AKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASPGDFAAVQKIAENVGGEEGPIICGLSR
ASKPDIKACANAIAPAPKKRIHTFIATSDIHLEHKLRKSRKEVLDIVPDMVGYAKSFVDDVEFSCEDAARSDLDFLYEVI
ELAISSGANTINIPDTVGYITPSEFGDLILNINENVPNINEAVLSVHGHNDLGLAVANFLEAVKNGARQLECTINGIGER
AGNAALEELIMALHVRRSYFNPFFGRPPESPTPLTAVRTEEITKSSRLVSNLTGMVVQPNKAIVGANAFAHESGIHQDGV
LKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLEDLGYDLNREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQ
LPEALFQLKLVQVSCGTSLMPTATVTVVGEDGEEKTAVSLGTGPVDAVVRALDSLTEEPNELIEFSVKSVTEGIDALGEV
TIRIRRDGNLFSGHSADTDVVVAAAQAYINALNRLVAAHGRKSIHPQHDLAKVDKKGI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=516, Percent_Identity=46.7054263565892, Blast_Score=437, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6320019, Length=403, Percent_Identity=28.7841191066998, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320071, Length=392, Percent_Identity=28.5714285714286, Blast_Score=133, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=502, Percent_Identity=25.8964143426295, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324682, Length=390, Percent_Identity=25.6410256410256, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_PROM1 (A2C3L7)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015342.1
- ProteinModelPortal:   A2C3L7
- SMR:   A2C3L7
- STRING:   A2C3L7
- GeneID:   4780698
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_15201
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   PMAR167555:NATL1_15201-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 58092; Mature: 57961

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASPGDFAA
CCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHH
VQKIAENVGGEEGPIICGLSRASKPDIKACANAIAPAPKKRIHTFIATSDIHLEHKLRKS
HHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHH
RKEVLDIVPDMVGYAKSFVDDVEFSCEDAARSDLDFLYEVIELAISSGANTINIPDTVGY
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
ITPSEFGDLILNINENVPNINEAVLSVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCHHCCCCEEEEECCCCCCCCHHHEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECHHH
RAGNAALEELIMALHVRRSYFNPFFGRPPESPTPLTAVRTEEITKSSRLVSNLTGMVVQP
HCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEECC
NKAIVGANAFAHESGIHQDGVLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLED
CCEEEECCHHHHHCCCCCCCCHHCCCEEEEECCEEECCCCCCEEECCCCCHHHHHHHHHH
LGYDLNREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQLPEALFQLKLVQVSCGTSL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
MPTATVTVVGEDGEEKTAVSLGTGPVDAVVRALDSLTEEPNELIEFSVKSVTEGIDALGE
CCCEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCE
VTIRIRRDGNLFSGHSADTDVVVAAAQAYINALNRLVAAHGRKSIHPQHDLAKVDKKGI
EEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
AKDPGRVLIFDTTLRDGEQSPGASLNLEEKLAIAQQLARLGVDVIEAGFPFASPGDFAA
CCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHH
VQKIAENVGGEEGPIICGLSRASKPDIKACANAIAPAPKKRIHTFIATSDIHLEHKLRKS
HHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHEECCHHHHHHHHHH
RKEVLDIVPDMVGYAKSFVDDVEFSCEDAARSDLDFLYEVIELAISSGANTINIPDTVGY
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
ITPSEFGDLILNINENVPNINEAVLSVHGHNDLGLAVANFLEAVKNGARQLECTINGIGE
CCHHCCCCEEEEECCCCCCCCHHHEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECHHH
RAGNAALEELIMALHVRRSYFNPFFGRPPESPTPLTAVRTEEITKSSRLVSNLTGMVVQP
HCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCEEECC
NKAIVGANAFAHESGIHQDGVLKNRLTYEIIDAKTVGLSDNKISLGKLSGRSAVRARLED
CCEEEECCHHHHHCCCCCCCCHHCCCEEEEECCEEECCCCCCEEECCCCCHHHHHHHHHH
LGYDLNREDLNDAFARFKDLADRKREITDRDLEAIVSEQVQLPEALFQLKLVQVSCGTSL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
MPTATVTVVGEDGEEKTAVSLGTGPVDAVVRALDSLTEEPNELIEFSVKSVTEGIDALGE
CCCEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCE
VTIRIRRDGNLFSGHSADTDVVVAAAQAYINALNRLVAAHGRKSIHPQHDLAKVDKKGI
EEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA