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The map label for this gene is cobB [H]

Identifier: 124026218

GI number: 124026218

Start: 1225579

End: 1226934

Strand: Direct

Name: cobB [H]

Synonym: NATL1_15121

Alternate gene names: 124026218

Gene position: 1225579-1226934 (Clockwise)

Preceding gene: 124026217

Following gene: 124026223

Centisome position: 65.72

GC content: 33.92

Gene sequence:

>1356_bases
ATGGCATGCGTAATCTCTGCAATATCAAGTAACAGTGGTAAAACTCTTTTAAGTCTTCTTTTAATTTCTTGGTTAAAAAG
TATAAATAAAACAGTTCAAACCTTTAAAGTTGGGCCTGATTATTTAGACCCTCAACAACTCACTGCAGTTTCAAAAAAAG
CTTGTCGCAATCTTGATTTAGTTCTCTCTGGTGAAAGTTGGGTTAAAGAAAATTTCAATCACTACGGAGGCTTAACTGAT
TATTCGTTTGTTGAAGGAGTAATGGGATTATTCGATGGAATTGGCAGTAGCTCAAAGGGGAGCACCGCTGAGTTAGCTAA
GGTTTTAAACCTGCCAGTAGTCCTTATTGTTGATGCTAGAGGGAAGGCTGCATCACTAGCAGCCTTAATAAAAGGATTTA
GAGAGCACGATAAAGAATTAAAAATTGCAGGCGTTGTTCTCAATAATGTTCAAACTACTAGACATGAAAAAATATTATTT
GAGGTTCTTGATCAAATCAATATAAAGTCATTAGGTTCTCTTCCGTTATGCAAAGACTTATATCTTCCTGCAAGGGATTT
AGGTTTAGCTCCGGCTCATGAAATTTTAGACTTAAATATTAAAGTTAAAAAGTGGACATCAATAGCCAAAGATTATCTAG
ATATAGAAAGTTTTAGAAAGCTTTTGTTACCTCCAGAACCTAACAACAAAACAGTTAGCTTTTTACCAAAAAAAGAATCA
GGCTTTATGCACCCAATAGCTATCGCTGAAGATGAAGCTTTCCATTTCAGGTATCAAGAAACAAAAGAATTATTAGAAGA
TAACGGAATGCCAACTATTACTTGGAAACCTCTTGAAAACGAACAGATCCCAAAAGAAGCTAGAGGATTAATAATACCTG
GAGGTTTCCCTGAGCAACATGCACACCAATTGAGTAATTCAAGAATAAGCATGAACTCAATAAAAATATTTTCGAAAAAG
TATCCTATATATGCTGAATGTGGTGGAATGATGCTTTTAGGTGAAAGTATCTTTGATATTGAAGGGAGAGAATATTCAAT
GGCTGGAATATTACCTTTTAAGGCTAAAAAGGGTAATTTAAAAATTGGTTATAGAGAAGCAACAAGTAAAAACAAAAGTC
CTATTACCACTCTTGGCAGTAAGTTAATAGGACATGAATTTCATCGCTGGGAAATAATTAATGAACGGTATAATTCAAAA
ATAAATCCACTATGGGATCTTAAAGGATGGAATATAAAGATTAAAAATGAAGGTTTTTGCAATCATTTAATTCATGCAAG
CTGGGTACATCTGCATTGGGCAAGTTCGCCTCTTATTCTAGAAAATTGGAAAAGAAGTGTTATGAATAAAGTCTAA

Upstream 100 bases:

>100_bases
AGTAGCAGAGGCAATACGAGTCCTTTACTTGAGAGTGCGACACCAATAGCAAAAGAGATGCTTGATTTAGTTAATCAAGC
AAACTAAATATAGTCATTAG

Downstream 100 bases:

>100_bases
AGCAAATCTATTTTTATAAATAACATTTTACAACTCAATCCATTAATAATTTCTCTAAAAGAGCTTGATTCATCTTGCTT
TTATTATCACCAACGATCCA

Product: putative cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MACVISAISSNSGKTLLSLLLISWLKSINKTVQTFKVGPDYLDPQQLTAVSKKACRNLDLVLSGESWVKENFNHYGGLTD
YSFVEGVMGLFDGIGSSSKGSTAELAKVLNLPVVLIVDARGKAASLAALIKGFREHDKELKIAGVVLNNVQTTRHEKILF
EVLDQINIKSLGSLPLCKDLYLPARDLGLAPAHEILDLNIKVKKWTSIAKDYLDIESFRKLLLPPEPNNKTVSFLPKKES
GFMHPIAIAEDEAFHFRYQETKELLEDNGMPTITWKPLENEQIPKEARGLIIPGGFPEQHAHQLSNSRISMNSIKIFSKK
YPIYAECGGMMLLGESIFDIEGREYSMAGILPFKAKKGNLKIGYREATSKNKSPITTLGSKLIGHEFHRWEIINERYNSK
INPLWDLKGWNIKIKNEGFCNHLIHASWVHLHWASSPLILENWKRSVMNKV

Sequences:

>Translated_451_residues
MACVISAISSNSGKTLLSLLLISWLKSINKTVQTFKVGPDYLDPQQLTAVSKKACRNLDLVLSGESWVKENFNHYGGLTD
YSFVEGVMGLFDGIGSSSKGSTAELAKVLNLPVVLIVDARGKAASLAALIKGFREHDKELKIAGVVLNNVQTTRHEKILF
EVLDQINIKSLGSLPLCKDLYLPARDLGLAPAHEILDLNIKVKKWTSIAKDYLDIESFRKLLLPPEPNNKTVSFLPKKES
GFMHPIAIAEDEAFHFRYQETKELLEDNGMPTITWKPLENEQIPKEARGLIIPGGFPEQHAHQLSNSRISMNSIKIFSKK
YPIYAECGGMMLLGESIFDIEGREYSMAGILPFKAKKGNLKIGYREATSKNKSPITTLGSKLIGHEFHRWEIINERYNSK
INPLWDLKGWNIKIKNEGFCNHLIHASWVHLHWASSPLILENWKRSVMNKV
>Mature_450_residues
ACVISAISSNSGKTLLSLLLISWLKSINKTVQTFKVGPDYLDPQQLTAVSKKACRNLDLVLSGESWVKENFNHYGGLTDY
SFVEGVMGLFDGIGSSSKGSTAELAKVLNLPVVLIVDARGKAASLAALIKGFREHDKELKIAGVVLNNVQTTRHEKILFE
VLDQINIKSLGSLPLCKDLYLPARDLGLAPAHEILDLNIKVKKWTSIAKDYLDIESFRKLLLPPEPNNKTVSFLPKKESG
FMHPIAIAEDEAFHFRYQETKELLEDNGMPTITWKPLENEQIPKEARGLIIPGGFPEQHAHQLSNSRISMNSIKIFSKKY
PIYAECGGMMLLGESIFDIEGREYSMAGILPFKAKKGNLKIGYREATSKNKSPITTLGSKLIGHEFHRWEIINERYNSKI
NPLWDLKGWNIKIKNEGFCNHLIHASWVHLHWASSPLILENWKRSVMNKV

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 50630; Mature: 50499

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACVISAISSNSGKTLLSLLLISWLKSINKTVQTFKVGPDYLDPQQLTAVSKKACRNLDL
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCEE
VLSGESWVKENFNHYGGLTDYSFVEGVMGLFDGIGSSSKGSTAELAKVLNLPVVLIVDAR
EECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECC
GKAASLAALIKGFREHDKELKIAGVVLNNVQTTRHEKILFEVLDQINIKSLGSLPLCKDL
CCHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHCCCCCCHHHC
YLPARDLGLAPAHEILDLNIKVKKWTSIAKDYLDIESFRKLLLPPEPNNKTVSFLPKKES
CCCHHHCCCCCCCCEEEEEEEEHHHHHHHHHHHCHHHHHHHCCCCCCCCCEEEEEECCCC
GFMHPIAIAEDEAFHFRYQETKELLEDNGMPTITWKPLENEQIPKEARGLIIPGGFPEQH
CCCCCEEEECCCHHEEHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHCCEEECCCCCHHH
AHQLSNSRISMNSIKIFSKKYPIYAECGGMMLLGESIFDIEGREYSMAGILPFKAKKGNL
HHHHCCCCEEEEEEEEECCCCCEEECCCCEEEECCCEEECCCCEEEEEEEEEEECCCCCE
KIGYREATSKNKSPITTLGSKLIGHEFHRWEIINERYNSKINPLWDLKGWNIKIKNEGFC
EEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCH
NHLIHASWVHLHWASSPLILENWKRSVMNKV
HHHHHCEEEEEEECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ACVISAISSNSGKTLLSLLLISWLKSINKTVQTFKVGPDYLDPQQLTAVSKKACRNLDL
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCCEE
VLSGESWVKENFNHYGGLTDYSFVEGVMGLFDGIGSSSKGSTAELAKVLNLPVVLIVDAR
EECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECC
GKAASLAALIKGFREHDKELKIAGVVLNNVQTTRHEKILFEVLDQINIKSLGSLPLCKDL
CCHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCHHHCCCCCCHHHC
YLPARDLGLAPAHEILDLNIKVKKWTSIAKDYLDIESFRKLLLPPEPNNKTVSFLPKKES
CCCHHHCCCCCCCCEEEEEEEEHHHHHHHHHHHCHHHHHHHCCCCCCCCCEEEEEECCCC
GFMHPIAIAEDEAFHFRYQETKELLEDNGMPTITWKPLENEQIPKEARGLIIPGGFPEQH
CCCCCEEEECCCHHEEHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHCCEEECCCCCHHH
AHQLSNSRISMNSIKIFSKKYPIYAECGGMMLLGESIFDIEGREYSMAGILPFKAKKGNL
HHHHCCCCEEEEEEEEECCCCCEEECCCCEEEECCCEEECCCCEEEEEEEEEEECCCCCE
KIGYREATSKNKSPITTLGSKLIGHEFHRWEIINERYNSKINPLWDLKGWNIKIKNEGFC
EEEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCCEEEECCCCCH
NHLIHASWVHLHWASSPLILENWKRSVMNKV
HHHHHCEEEEEEECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA