Click here to switch to the map view.

The map label for this gene is gldA [C]

Identifier: 124026199

GI number: 124026199

Start: 1204858

End: 1205976

Strand: Direct

Name: gldA [C]

Synonym: NATL1_14931

Alternate gene names: 124026199

Gene position: 1204858-1205976 (Clockwise)

Preceding gene: 124026198

Following gene: 124026200

Centisome position: 64.61

GC content: 36.55

Gene sequence:

>1119_bases
ATGTTTATAAATAATCACTGCATTTCACCTTCAAATGTCGTTAGAGGTGAAGGAGCATGGGGTGACTCATTAGATCTAAT
AATTAAATTATGCAAAAGACCTTTAATAATAGGTAGAAGTAGCTCTACAAAAAAAATAAGAACTTCATTCAAAAAAGATC
TTCTTTTAAAAGGTATTCAATCAATCTCTCTCGATTTAGATCATGATTGCTGTGAATTAGATATCCAAAATGCATATCTA
ATCTCAAAACACAATAACTGTGATGGGATTATTGCTGCAGGAGGTGGGAAAGTACTTGACGCAGGAAAACTAATCGCTGA
TTTACTTGGTATTAATTGCATAACTGTTCCATTAAGTGCCTCCACATGTGCTGGATGGACCGCTTTATCTAATATTTATA
CTCCCGATGGAAAATTTGTAAAAGATGTAACTTTGAAAAGCTGTCCAAACTTATTGATCTTTGATCACACAATTGTTAGA
AACGCCCCACCAAGAACACTTGCTAGCGGCATGGCTGATGCTGTCGCAAAATGGTACGAGTCTTCCCTAACAAGCAGTTC
AAGCCAAGATGGCTTTGTTCAGCAAGCAGTTCAGATGGCTAGGGTTTTACGCGATCAATTATTTTTAAACGGTCAAAAGG
CTTTCTTGGATCCATTAAGCAATTCTTGGGAAACTGTTGCAGAAGGATGTGCCTTAACTGCTGGGATAATAGGAGGGCTT
GGGGGCGCAAGATGCAGAACAGCCGCAGCTCATCCTATACACAATGGATTAACACAACTCGCATACACTAACAAACCACT
TCATGGAGAACTTGTTGGATTTGGCTTACTCGTGCAATTACATCTAGAAGAAAAAAATTCAAACAGTCAATTACCAAAGC
AAGCTAAATCACAACTTTTAGAGTTCTTCTCGCAACTCAATCTCCCTATTTCAATTGAGTCTATTTGTCTTAGAAATACT
ACCTCGAATGAACTATATAAAGCATGTAAATTCGCCTGTAATGATGACTCTGATATACATAAATTACCTTTCCCAATAAA
TGAAAAAGACATTTTAGAGGCTATTCAAAGTTTACAATCCTTGCCAACAACCTCCAGAATAAAAATAAATAATACTTAA

Upstream 100 bases:

>100_bases
TTGATGAAAGCAAGAAAGTAATTGTTAAACATCTTGGGAAAGATGGTTCCAAACCAGAATTAGCAAGCGCCACTGTTTAA
CTTTTAAATAGAAAAGTTAT

Downstream 100 bases:

>100_bases
GAGCATTGAATCAAGAAGAAAAGAAAAATATAGATACAACAAATGCTACGAAGGCATATATAAATGAAATACGAAATCAA
ATTATAGACAATCAAGCTAG

Product: putative glycerol dehydrogenase

Products: glycerone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MFINNHCISPSNVVRGEGAWGDSLDLIIKLCKRPLIIGRSSSTKKIRTSFKKDLLLKGIQSISLDLDHDCCELDIQNAYL
ISKHNNCDGIIAAGGGKVLDAGKLIADLLGINCITVPLSASTCAGWTALSNIYTPDGKFVKDVTLKSCPNLLIFDHTIVR
NAPPRTLASGMADAVAKWYESSLTSSSSQDGFVQQAVQMARVLRDQLFLNGQKAFLDPLSNSWETVAEGCALTAGIIGGL
GGARCRTAAAHPIHNGLTQLAYTNKPLHGELVGFGLLVQLHLEEKNSNSQLPKQAKSQLLEFFSQLNLPISIESICLRNT
TSNELYKACKFACNDDSDIHKLPFPINEKDILEAIQSLQSLPTTSRIKINNT

Sequences:

>Translated_372_residues
MFINNHCISPSNVVRGEGAWGDSLDLIIKLCKRPLIIGRSSSTKKIRTSFKKDLLLKGIQSISLDLDHDCCELDIQNAYL
ISKHNNCDGIIAAGGGKVLDAGKLIADLLGINCITVPLSASTCAGWTALSNIYTPDGKFVKDVTLKSCPNLLIFDHTIVR
NAPPRTLASGMADAVAKWYESSLTSSSSQDGFVQQAVQMARVLRDQLFLNGQKAFLDPLSNSWETVAEGCALTAGIIGGL
GGARCRTAAAHPIHNGLTQLAYTNKPLHGELVGFGLLVQLHLEEKNSNSQLPKQAKSQLLEFFSQLNLPISIESICLRNT
TSNELYKACKFACNDDSDIHKLPFPINEKDILEAIQSLQSLPTTSRIKINNT
>Mature_372_residues
MFINNHCISPSNVVRGEGAWGDSLDLIIKLCKRPLIIGRSSSTKKIRTSFKKDLLLKGIQSISLDLDHDCCELDIQNAYL
ISKHNNCDGIIAAGGGKVLDAGKLIADLLGINCITVPLSASTCAGWTALSNIYTPDGKFVKDVTLKSCPNLLIFDHTIVR
NAPPRTLASGMADAVAKWYESSLTSSSSQDGFVQQAVQMARVLRDQLFLNGQKAFLDPLSNSWETVAEGCALTAGIIGGL
GGARCRTAAAHPIHNGLTQLAYTNKPLHGELVGFGLLVQLHLEEKNSNSQLPKQAKSQLLEFFSQLNLPISIESICLRNT
TSNELYKACKFACNDDSDIHKLPFPINEKDILEAIQSLQSLPTTSRIKINNT

Specific function: Glycerol utilization. [C]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=344, Percent_Identity=28.7790697674419, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1786815, Length=370, Percent_Identity=27.027027027027, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.6

Molecular weight: Translated: 40346; Mature: 40346

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFINNHCISPSNVVRGEGAWGDSLDLIIKLCKRPLIIGRSSSTKKIRTSFKKDLLLKGIQ
CEECCCCCCCCCEECCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHH
SISLDLDHDCCELDIQNAYLISKHNNCDGIIAAGGGKVLDAGKLIADLLGINCITVPLSA
HHCCCCCCCHHEEECCCEEEEEECCCCCEEEECCCCCEEHHHHHHHHHHCCCEEEEECCC
STCAGWTALSNIYTPDGKFVKDVTLKSCPNLLIFDHTIVRNAPPRTLASGMADAVAKWYE
CHHHHHHHHHCCCCCCCCEEHHHHHHHCCCEEEECHHHHCCCCCHHHHHHHHHHHHHHHH
SSLTSSSSQDGFVQQAVQMARVLRDQLFLNGQKAFLDPLSNSWETVAEGCALTAGIIGGL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCHHCCCHHHHHHHHHHHHHHHHCC
GGARCRTAAAHPIHNGLTQLAYTNKPLHGELVGFGLLVQLHLEEKNSNSQLPKQAKSQLL
CCHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHEEEEEEEEEECCCCCCCCCHHHHHHHH
EFFSQLNLPISIESICLRNTTSNELYKACKFACNDDSDIHKLPFPINEKDILEAIQSLQS
HHHHHCCCCEEHHHHEECCCCHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHH
LPTTSRIKINNT
CCCCCEEEECCC
>Mature Secondary Structure
MFINNHCISPSNVVRGEGAWGDSLDLIIKLCKRPLIIGRSSSTKKIRTSFKKDLLLKGIQ
CEECCCCCCCCCEECCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHH
SISLDLDHDCCELDIQNAYLISKHNNCDGIIAAGGGKVLDAGKLIADLLGINCITVPLSA
HHCCCCCCCHHEEECCCEEEEEECCCCCEEEECCCCCEEHHHHHHHHHHCCCEEEEECCC
STCAGWTALSNIYTPDGKFVKDVTLKSCPNLLIFDHTIVRNAPPRTLASGMADAVAKWYE
CHHHHHHHHHCCCCCCCCEEHHHHHHHCCCEEEECHHHHCCCCCHHHHHHHHHHHHHHHH
SSLTSSSSQDGFVQQAVQMARVLRDQLFLNGQKAFLDPLSNSWETVAEGCALTAGIIGGL
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCHHCCCHHHHHHHHHHHHHHHHCC
GGARCRTAAAHPIHNGLTQLAYTNKPLHGELVGFGLLVQLHLEEKNSNSQLPKQAKSQLL
CCHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHEEEEEEEEEECCCCCCCCCHHHHHHHH
EFFSQLNLPISIESICLRNTTSNELYKACKFACNDDSDIHKLPFPINEKDILEAIQSLQS
HHHHHCCCCEEHHHHEECCCCHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHH
LPTTSRIKINNT
CCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.13 {NAD+}} 0.12 {NADH}} 0.06 {dihydroxyacetone}} 0.5 {glycerol}} [C]

Substrates: glycerol; NAD+

Specific reaction: glycerol + NAD+ = glycerone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Chelatingagents; Li+; Na+; Thiolcompounds [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]