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The map label for this gene is bioB

Identifier: 124026193

GI number: 124026193

Start: 1197337

End: 1198374

Strand: Reverse

Name: bioB

Synonym: NATL1_14871

Alternate gene names: 124026193

Gene position: 1198374-1197337 (Counterclockwise)

Preceding gene: 124026194

Following gene: 124026192

Centisome position: 64.27

GC content: 40.46

Gene sequence:

>1038_bases
ATGACTCTAATTAATCCTAATATCCAGGAATCTAATAAACTCAAGTTCAAAGACGAATCATATTTAGATTTTAATTCCAT
AAATGGTGGAGATATTAGACATGATTGGTCTTCAGAAGAAATCAAAGAAATACTTGATTTGCCGTTAATGGATTTGTTGT
GGAGAGCTCAAATAGTTCATAGGTCTTACAATCCCGGTTATAAAGTTCAGCTTGCTTCTCTTCTAAGTGTGAAGACAGGT
GGATGCTCAGAAGACTGTGCATATTGTCCTCAATCTGTTCACAATGAAACAACTGTTCAACCTAATCCTGTAATTGAAGT
TGAGTCAGTTCTTGATAGAGCAAGAGCTGCAAAAGATGCAGGAGCAGATAGATTTTGCATGGGTTGGGCTTGGCGTGAGA
TCAAAGACGGAAACGCATTCGATTCAATGCTTGAAATGGTAAGAGGTGTTAGAGAGCTTGGCCTTGAGGCATGTGTCACC
GCTGGAATGATTACTGATTCTCAAGCCTCTAGATTGGCAGAAGCAGGTTTAACAGCCTATAACCATAATTTAGATACTAG
TCCTGAGCATTATTCCAAAATCATTTCAACAAGAACATATCAAGATCGACTTGAAACATTGAGAAGAGTACGCATGGCTG
GAATTACAGTGTGCTGTGGTGGGATTATTGGCATGGGGGAATCTGTTTCAGATAGAGCATCTTTACTTAAGGTTTTAGCA
ACTTTAGACCCGCATCCTGAAAGTGTACCTATTAATGCGTTGGTTGCAGTGGAGGGGACACCCATGGAGGATTTGTCTTC
TATCGATCCATTAGAGATGGTTCGTATGGTCGCGACGGCAAGGGTTATTATGCCTAAAAGCCGAATAAGACTTAGCGCAG
GGAGACAACAATTAGGTAGGGAAGCTCAGATTCTATGTCTACAATCTGGAGCTGATTCTATATTTTATGGAGATACACTT
TTAACTACAAGCAATCCGGAGGTGGAAGCAGACCGTAAGCTTTTAGCGGATGCTGGAATTACGGCTAATTTCTCTTAA

Upstream 100 bases:

>100_bases
TGTATAACTAAATTAATTGAATATTTTGAGTTGTATGATTTTTTATAGATTATCGGCTAAAACTAAATAGCAATTAAATT
TCTTATATTCAAATTCTTTT

Downstream 100 bases:

>100_bases
GAATAAGTAGGTATGAAAAAAGATGATAGCCTTAAGAAATTTAAATATAAAGTTGCTGCGTTTTACAATTTTATATCGAT
TATTGATGAAGAAATTCTTT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTLINPNIQESNKLKFKDESYLDFNSINGGDIRHDWSSEEIKEILDLPLMDLLWRAQIVHRSYNPGYKVQLASLLSVKTG
GCSEDCAYCPQSVHNETTVQPNPVIEVESVLDRARAAKDAGADRFCMGWAWREIKDGNAFDSMLEMVRGVRELGLEACVT
AGMITDSQASRLAEAGLTAYNHNLDTSPEHYSKIISTRTYQDRLETLRRVRMAGITVCCGGIIGMGESVSDRASLLKVLA
TLDPHPESVPINALVAVEGTPMEDLSSIDPLEMVRMVATARVIMPKSRIRLSAGRQQLGREAQILCLQSGADSIFYGDTL
LTTSNPEVEADRKLLADAGITANFS

Sequences:

>Translated_345_residues
MTLINPNIQESNKLKFKDESYLDFNSINGGDIRHDWSSEEIKEILDLPLMDLLWRAQIVHRSYNPGYKVQLASLLSVKTG
GCSEDCAYCPQSVHNETTVQPNPVIEVESVLDRARAAKDAGADRFCMGWAWREIKDGNAFDSMLEMVRGVRELGLEACVT
AGMITDSQASRLAEAGLTAYNHNLDTSPEHYSKIISTRTYQDRLETLRRVRMAGITVCCGGIIGMGESVSDRASLLKVLA
TLDPHPESVPINALVAVEGTPMEDLSSIDPLEMVRMVATARVIMPKSRIRLSAGRQQLGREAQILCLQSGADSIFYGDTL
LTTSNPEVEADRKLLADAGITANFS
>Mature_344_residues
TLINPNIQESNKLKFKDESYLDFNSINGGDIRHDWSSEEIKEILDLPLMDLLWRAQIVHRSYNPGYKVQLASLLSVKTGG
CSEDCAYCPQSVHNETTVQPNPVIEVESVLDRARAAKDAGADRFCMGWAWREIKDGNAFDSMLEMVRGVRELGLEACVTA
GMITDSQASRLAEAGLTAYNHNLDTSPEHYSKIISTRTYQDRLETLRRVRMAGITVCCGGIIGMGESVSDRASLLKVLAT
LDPHPESVPINALVAVEGTPMEDLSSIDPLEMVRMVATARVIMPKSRIRLSAGRQQLGREAQILCLQSGADSIFYGDTLL
TTSNPEVEADRKLLADAGITANFS

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=308, Percent_Identity=52.2727272727273, Blast_Score=340, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6321725, Length=316, Percent_Identity=46.8354430379747, Blast_Score=310, Evalue=3e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_PROM1 (A2C3I4)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015309.1
- ProteinModelPortal:   A2C3I4
- SMR:   A2C3I4
- STRING:   A2C3I4
- GeneID:   4779672
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_14871
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2391510
- BioCyc:   PMAR167555:NATL1_14871-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37939; Mature: 37807

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLINPNIQESNKLKFKDESYLDFNSINGGDIRHDWSSEEIKEILDLPLMDLLWRAQIVH
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHH
RSYNPGYKVQLASLLSVKTGGCSEDCAYCPQSVHNETTVQPNPVIEVESVLDRARAAKDA
CCCCCCCEEEHHHHHHHCCCCCCHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
GADRFCMGWAWREIKDGNAFDSMLEMVRGVRELGLEACVTAGMITDSQASRLAEAGLTAY
CCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHC
NHNLDTSPEHYSKIISTRTYQDRLETLRRVRMAGITVCCGGIIGMGESVSDRASLLKVLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TLDPHPESVPINALVAVEGTPMEDLSSIDPLEMVRMVATARVIMPKSRIRLSAGRQQLGR
HCCCCCCCCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
EAQILCLQSGADSIFYGDTLLTTSNPEVEADRKLLADAGITANFS
CEEEEEEECCCCCEEECCEEEECCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TLINPNIQESNKLKFKDESYLDFNSINGGDIRHDWSSEEIKEILDLPLMDLLWRAQIVH
CCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHH
RSYNPGYKVQLASLLSVKTGGCSEDCAYCPQSVHNETTVQPNPVIEVESVLDRARAAKDA
CCCCCCCEEEHHHHHHHCCCCCCHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
GADRFCMGWAWREIKDGNAFDSMLEMVRGVRELGLEACVTAGMITDSQASRLAEAGLTAY
CCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHC
NHNLDTSPEHYSKIISTRTYQDRLETLRRVRMAGITVCCGGIIGMGESVSDRASLLKVLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
TLDPHPESVPINALVAVEGTPMEDLSSIDPLEMVRMVATARVIMPKSRIRLSAGRQQLGR
HCCCCCCCCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCC
EAQILCLQSGADSIFYGDTLLTTSNPEVEADRKLLADAGITANFS
CEEEEEEECCCCCEEECCEEEECCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA