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The map label for this gene is pykF [H]

Identifier: 124025674

GI number: 124025674

Start: 888112

End: 889569

Strand: Reverse

Name: pykF [H]

Synonym: NATL1_09671

Alternate gene names: 124025674

Gene position: 889569-888112 (Counterclockwise)

Preceding gene: 124025676

Following gene: 124025673

Centisome position: 47.7

GC content: 38.34

Gene sequence:

>1458_bases
ATGAAAATACTCGACCTAGCCAGAAGGACCAAGATTGTAGCTACGATAGGACCCGCAACTGAGAGTGCGAGTCAAATAAG
CAAGTTAGTAGAGGCCGGTGCAACTACTTTCCGTCTAAATTTTAGTCATGGTGATCATGAAGAACATGCTCAAAGAATAA
AAACAATTAGAAAAGTTTCTGACGATCTAGGCATTCACATAGGAATCCTTCAGGATCTTCAGGGGCCTAAGATTAGACTT
GGTAGATTTAAAAATGGCCCAGTTAATGTCAAAAATGGCGATAAATTTATTCTTACATCGAAAAATATTGAATGTAATCA
GGACCAAGCAAATGTCACTTACGACAAGCTAGCTGAAGAAGTAAATGTTGGAAATCGAATTCTGTTGGATGATGGAAGGG
TAGAGATGAAAGTTGAAGAAGTAGATATAAAAGAACAAAGATTAATTTGTTCAATAACTGTGGGTGGGGTTCTATCTAAT
AACAAAGGAGTTAATTTCCCTGATGTTCAACTCTCCATAAGGGCTTTAACAGACAAAGATAAAAAAGATCTAGCATTTGG
TTTAAAACAAGGTGTTGATTGGGTAGCACTAAGTTTTGTAAGAAATCCTTCTGATTTAATAGAAATAAAAGATTTAATTA
GAAATCATGGATATGACACTCCAGTAGTTGCGAAGATAGAAAAATTTGAAGCAATTGATCAAATAGACGCTGTCTTAAAA
TTATGTGATGGAGTCATGGTTGCTAGAGGTGATCTCGGTGTAGAGATGCCAGCAGAAGAAGTTCCACTATTACAAAAACA
ACTTATCAAAAAGGCAAACAGCCTTGGCATACCAATAATTACAGCCACACAAATGCTTGATTCAATGGCCTCAAGCCCAA
GGCCTACAAGAGCAGAGGTGAGTGATGTTGCAAATGCAATACTCGATGGCACGGATGCTGTAATGCTCTCAAATGAGACT
GCAGTGGGTGATTATCCCATTGAGGCTGTGGCGACTATGGCTACTATTGCGAAAAGAATCGAACGAGATTATCCACAAAA
AGCATTAGAAAGTCATCTTCCAAGCACTATTCCAAATGCTATTAGTGCGGCTGTAAGCAGTATTGCTAGACAAATAAACG
CTGCCGCAATAATACCTTTAACTAAAAGTGGTGCTACCGCCAAAAACGTAAGCAAGTTTAGACCTCCCACTCCAGTATTA
GCAGTAACTAATGAAAAGAGTGTTGCCAGCACACTTCAACTTGTCTGGGGAGTAACGCCCCTTCTAATAGAAAATCAAAC
AAGCACATCAAAAACATTTGTTGATGCCATGAATATGGCCAAAAAAATGAAAATCCTCAAGCAAGGAGATCTTGTTGTTG
AGACAGCTGGAACACTATCAGGGGTAAGTGGGTCAACAGACTTGGTAAAGGTTGGGATAGTTAGTTCAGAAGATGAAAAT
GAATCTTTATTTATCTAA

Upstream 100 bases:

>100_bases
TTTGATAATCATTTAATTCCATCTTTATTCTCCTGAAAAAGTAAAGTTGACAAATAAAACACAATCAGATGTTAGTTTTT
TGTTATCACAAGTCACATAA

Downstream 100 bases:

>100_bases
AAACATATGAAAAGAAAACTCCCGCTTGCTGAAACTTCGGGAATGGCAATAAAGAATCTTAAGTCAAATAAGTTTAGAAG
CTTATTAACAATGCTAGGAA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKILDLARRTKIVATIGPATESASQISKLVEAGATTFRLNFSHGDHEEHAQRIKTIRKVSDDLGIHIGILQDLQGPKIRL
GRFKNGPVNVKNGDKFILTSKNIECNQDQANVTYDKLAEEVNVGNRILLDDGRVEMKVEEVDIKEQRLICSITVGGVLSN
NKGVNFPDVQLSIRALTDKDKKDLAFGLKQGVDWVALSFVRNPSDLIEIKDLIRNHGYDTPVVAKIEKFEAIDQIDAVLK
LCDGVMVARGDLGVEMPAEEVPLLQKQLIKKANSLGIPIITATQMLDSMASSPRPTRAEVSDVANAILDGTDAVMLSNET
AVGDYPIEAVATMATIAKRIERDYPQKALESHLPSTIPNAISAAVSSIARQINAAAIIPLTKSGATAKNVSKFRPPTPVL
AVTNEKSVASTLQLVWGVTPLLIENQTSTSKTFVDAMNMAKKMKILKQGDLVVETAGTLSGVSGSTDLVKVGIVSSEDEN
ESLFI

Sequences:

>Translated_485_residues
MKILDLARRTKIVATIGPATESASQISKLVEAGATTFRLNFSHGDHEEHAQRIKTIRKVSDDLGIHIGILQDLQGPKIRL
GRFKNGPVNVKNGDKFILTSKNIECNQDQANVTYDKLAEEVNVGNRILLDDGRVEMKVEEVDIKEQRLICSITVGGVLSN
NKGVNFPDVQLSIRALTDKDKKDLAFGLKQGVDWVALSFVRNPSDLIEIKDLIRNHGYDTPVVAKIEKFEAIDQIDAVLK
LCDGVMVARGDLGVEMPAEEVPLLQKQLIKKANSLGIPIITATQMLDSMASSPRPTRAEVSDVANAILDGTDAVMLSNET
AVGDYPIEAVATMATIAKRIERDYPQKALESHLPSTIPNAISAAVSSIARQINAAAIIPLTKSGATAKNVSKFRPPTPVL
AVTNEKSVASTLQLVWGVTPLLIENQTSTSKTFVDAMNMAKKMKILKQGDLVVETAGTLSGVSGSTDLVKVGIVSSEDEN
ESLFI
>Mature_485_residues
MKILDLARRTKIVATIGPATESASQISKLVEAGATTFRLNFSHGDHEEHAQRIKTIRKVSDDLGIHIGILQDLQGPKIRL
GRFKNGPVNVKNGDKFILTSKNIECNQDQANVTYDKLAEEVNVGNRILLDDGRVEMKVEEVDIKEQRLICSITVGGVLSN
NKGVNFPDVQLSIRALTDKDKKDLAFGLKQGVDWVALSFVRNPSDLIEIKDLIRNHGYDTPVVAKIEKFEAIDQIDAVLK
LCDGVMVARGDLGVEMPAEEVPLLQKQLIKKANSLGIPIITATQMLDSMASSPRPTRAEVSDVANAILDGTDAVMLSNET
AVGDYPIEAVATMATIAKRIERDYPQKALESHLPSTIPNAISAAVSSIARQINAAAIIPLTKSGATAKNVSKFRPPTPVL
AVTNEKSVASTLQLVWGVTPLLIENQTSTSKTFVDAMNMAKKMKILKQGDLVVETAGTLSGVSGSTDLVKVGIVSSEDEN
ESLFI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=491, Percent_Identity=38.4928716904277, Blast_Score=320, Evalue=3e-87,
Organism=Homo sapiens, GI10835121, Length=491, Percent_Identity=38.4928716904277, Blast_Score=319, Evalue=3e-87,
Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=37.4233128834356, Blast_Score=318, Evalue=8e-87,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=37.4233128834356, Blast_Score=318, Evalue=8e-87,
Organism=Homo sapiens, GI33286418, Length=491, Percent_Identity=37.8818737270876, Blast_Score=318, Evalue=1e-86,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=36.551724137931, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=36.551724137931, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=32.7731092436975, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=32.7731092436975, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=31.4814814814815, Blast_Score=119, Evalue=7e-27,
Organism=Escherichia coli, GI1787965, Length=473, Percent_Identity=43.1289640591966, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1788160, Length=474, Percent_Identity=41.7721518987342, Blast_Score=335, Evalue=4e-93,
Organism=Caenorhabditis elegans, GI17544584, Length=481, Percent_Identity=38.2536382536383, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=38.477366255144, Blast_Score=297, Evalue=9e-81,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=38.477366255144, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI71984406, Length=486, Percent_Identity=38.477366255144, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17506831, Length=486, Percent_Identity=38.477366255144, Blast_Score=296, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6319279, Length=479, Percent_Identity=39.4572025052192, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=37.5770020533881, Blast_Score=293, Evalue=6e-80,
Organism=Drosophila melanogaster, GI24648964, Length=488, Percent_Identity=38.5245901639344, Blast_Score=309, Evalue=3e-84,
Organism=Drosophila melanogaster, GI28571814, Length=488, Percent_Identity=38.5245901639344, Blast_Score=309, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24648966, Length=436, Percent_Identity=39.4495412844037, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24581235, Length=509, Percent_Identity=33.9882121807466, Blast_Score=268, Evalue=5e-72,
Organism=Drosophila melanogaster, GI24646914, Length=298, Percent_Identity=36.9127516778523, Blast_Score=189, Evalue=4e-48,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52419; Mature: 52419

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILDLARRTKIVATIGPATESASQISKLVEAGATTFRLNFSHGDHEEHAQRIKTIRKVS
CCHHHHHHHCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHH
DDLGIHIGILQDLQGPKIRLGRFKNGPVNVKNGDKFILTSKNIECNQDQANVTYDKLAEE
HHCCEEEEEHHCCCCCCEEECCCCCCCEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHH
VNVGNRILLDDGRVEMKVEEVDIKEQRLICSITVGGVLSNNKGVNFPDVQLSIRALTDKD
HCCCCEEEEECCCEEEEEECCCCCCCEEEEEEEECCEEECCCCCCCCCCEEEEEECCCCC
KKDLAFGLKQGVDWVALSFVRNPSDLIEIKDLIRNHGYDTPVVAKIEKFEAIDQIDAVLK
HHHHHHHHHHCCCCEEHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LCDGVMVARGDLGVEMPAEEVPLLQKQLIKKANSLGIPIITATQMLDSMASSPRPTRAEV
HHCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCHHHH
SDVANAILDGTDAVMLSNETAVGDYPIEAVATMATIAKRIERDYPQKALESHLPSTIPNA
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHH
ISAAVSSIARQINAAAIIPLTKSGATAKNVSKFRPPTPVLAVTNEKSVASTLQLVWGVTP
HHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCC
LLIENQTSTSKTFVDAMNMAKKMKILKQGDLVVETAGTLSGVSGSTDLVKVGIVSSEDEN
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCC
ESLFI
CCCCC
>Mature Secondary Structure
MKILDLARRTKIVATIGPATESASQISKLVEAGATTFRLNFSHGDHEEHAQRIKTIRKVS
CCHHHHHHHCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHH
DDLGIHIGILQDLQGPKIRLGRFKNGPVNVKNGDKFILTSKNIECNQDQANVTYDKLAEE
HHCCEEEEEHHCCCCCCEEECCCCCCCEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHH
VNVGNRILLDDGRVEMKVEEVDIKEQRLICSITVGGVLSNNKGVNFPDVQLSIRALTDKD
HCCCCEEEEECCCEEEEEECCCCCCCEEEEEEEECCEEECCCCCCCCCCEEEEEECCCCC
KKDLAFGLKQGVDWVALSFVRNPSDLIEIKDLIRNHGYDTPVVAKIEKFEAIDQIDAVLK
HHHHHHHHHHCCCCEEHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LCDGVMVARGDLGVEMPAEEVPLLQKQLIKKANSLGIPIITATQMLDSMASSPRPTRAEV
HHCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCHHHH
SDVANAILDGTDAVMLSNETAVGDYPIEAVATMATIAKRIERDYPQKALESHLPSTIPNA
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHH
ISAAVSSIARQINAAAIIPLTKSGATAKNVSKFRPPTPVLAVTNEKSVASTLQLVWGVTP
HHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHCCCCCCEEEEECCHHHHHHHHHHHCCCC
LLIENQTSTSKTFVDAMNMAKKMKILKQGDLVVETAGTLSGVSGSTDLVKVGIVSSEDEN
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCC
ESLFI
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]