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The map label for this gene is ppx [H]

Identifier: 124025223

GI number: 124025223

Start: 466529

End: 468163

Strand: Direct

Name: ppx [H]

Synonym: NATL1_05121

Alternate gene names: 124025223

Gene position: 466529-468163 (Clockwise)

Preceding gene: 124025220

Following gene: 124025229

Centisome position: 25.02

GC content: 34.07

Gene sequence:

>1635_bases
ATGTTAGGTGACTCAATAAATAATTCCTCTGAAAGGAATTCTATTGAATTAGATCTTGGCGAAAAGCAAGATGCTGAATT
ATGCCGTGTGGCAACAATTGATATTGGCACCAACTCAACGCATTTATTAATTGCAAAGATTGAACGAAAATTAAATACTT
TTAGTATTGAACTTGCAGAGAAATCGACAACACGACTAGGAGAAAGAGATCCTCAGACAGGAGAACTTACATCCCTTGCT
ATGAATAGGGCTTTTTCAACATTAAAGAGGTTTAAGGATTTATCAGAAAGTTATAAAGTTGAGAGTCTTATTATTGCCGC
TACAAGTGCAGTTAGAGAAGCACCAAATGGGAAAATCTTTATTGCTGAAATTAAAAAGAAGATAGGCTTAGATGTTGAAT
TGATAAGCGGAGCAGAAGAAGCAAGATTGATTTATCTAGGTGTACTTTCAGGGATGCAATTTGGAAATAAACCTCATCTC
GTCCTAGACATTGGAGGAGGTTCGACAGAATTAATCCTTGCTGATAGCTCTGAGGCTCGAGCATTAACAAGTACAAAGAT
TGGAGCAGTAAGATTACAAAGAGAATTTATTAAAAAAGATCCAATATCCTCTCAAAATGAATTGTTTTTAAGGTCATTTA
TCAGAGGCTCTATGGAGTCTGCAATTGATAAAGTATCTAAGAGAATTGAAGCGGGAGAAATACCAGTTTTAGTAGCAACT
AGTGGAACTGCTATGGCTATTGGGTCGTTACTATCTAATAAAGAAAATCATATGCAATCAAAATTACAAGGATATAAAAT
CACAAAAAATAATTTAGATATAATTGTTAGTCAGTTAATTAAAATGACACCTTCTGAACGGAGTCAATTATCCTCATTAA
GTGAAAGAAGATCTGAGATTATTGTTCCAGGCGCTTTGATTTTGCAAACCATAATGAATATGGTCGATGTTAATGAAATT
ATTTTAAGTGAAAGAGCTCTTCGGGAAGGATTGGTTGTTGATTGGATGTGTCGAAATAATTATTTAAAAGATCAGCTGAG
CTTTCAAGGATCAATTAGAGAAAGAACAGTTATTCATCAAGCGAAAAGATTTGGTGTTAATTCAAAACGATCAAAAAGTG
TATCAGAATTTGCACTTACTTTTTATGATCAGACTAAGGGGGTTTTGCACAATGATAATGGTGGAGGTAGAGATCTTTTG
TGGGCAGCAGCTAAGCTTCACGCATGTGGTAAACAAATTAACATAAGTGCCTATCACAAGCATTCGTGGTATTTAATCAA
AAATGGAGAGCTCTTAGGTTATTCCCAAAGTGAGCATTTAATGGTTGCTGCTATTGCAAGGTATCACAGGAAAAGTTTTC
CAAAAAAAAGACATGAATCTTGGCAATTATTGGTTGATGAAAGTCAAAGAAGTTTGGTAGCTGATATGTCTTTACTTCTT
CGACTTTCATGCTCTTTGGATAGAAGGCCAGAACCTTTGGTATCCAAAATAGTTATTGAAGCTAATAACAAGAAAGTTAA
TATAGAGCTGATTCCTAATAACTTAGGCCAAAATTTAGATCTTGAGAAATGGAGCTTAACTAAGGCAATTTTACTTGTTA
AAAAGATTATGGATGTTGATATAAATATACTTTAA

Upstream 100 bases:

>100_bases
AAGAAGATTATTCACGAAAATTCAAATTGATAATGATTTATAAGGTCTTCTCAATGGGGTGCTGAGGGTGTCAGAGTATT
GAGGTTAAACCTACTTATTT

Downstream 100 bases:

>100_bases
GAAGATATCTTTTTGTCTAATAAAAAGATATCACTTCGCTGAGGATTAAGGGAATCATTTTGTCCTTTTTCAATATATTT
TAATAGCATAATTGAAGTAA

Product: putative exopolyphosphatase

Products: NA

Alternate protein names: ExopolyPase; Metaphosphatase [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MLGDSINNSSERNSIELDLGEKQDAELCRVATIDIGTNSTHLLIAKIERKLNTFSIELAEKSTTRLGERDPQTGELTSLA
MNRAFSTLKRFKDLSESYKVESLIIAATSAVREAPNGKIFIAEIKKKIGLDVELISGAEEARLIYLGVLSGMQFGNKPHL
VLDIGGGSTELILADSSEARALTSTKIGAVRLQREFIKKDPISSQNELFLRSFIRGSMESAIDKVSKRIEAGEIPVLVAT
SGTAMAIGSLLSNKENHMQSKLQGYKITKNNLDIIVSQLIKMTPSERSQLSSLSERRSEIIVPGALILQTIMNMVDVNEI
ILSERALREGLVVDWMCRNNYLKDQLSFQGSIRERTVIHQAKRFGVNSKRSKSVSEFALTFYDQTKGVLHNDNGGGRDLL
WAAAKLHACGKQINISAYHKHSWYLIKNGELLGYSQSEHLMVAAIARYHRKSFPKKRHESWQLLVDESQRSLVADMSLLL
RLSCSLDRRPEPLVSKIVIEANNKKVNIELIPNNLGQNLDLEKWSLTKAILLVKKIMDVDINIL

Sequences:

>Translated_544_residues
MLGDSINNSSERNSIELDLGEKQDAELCRVATIDIGTNSTHLLIAKIERKLNTFSIELAEKSTTRLGERDPQTGELTSLA
MNRAFSTLKRFKDLSESYKVESLIIAATSAVREAPNGKIFIAEIKKKIGLDVELISGAEEARLIYLGVLSGMQFGNKPHL
VLDIGGGSTELILADSSEARALTSTKIGAVRLQREFIKKDPISSQNELFLRSFIRGSMESAIDKVSKRIEAGEIPVLVAT
SGTAMAIGSLLSNKENHMQSKLQGYKITKNNLDIIVSQLIKMTPSERSQLSSLSERRSEIIVPGALILQTIMNMVDVNEI
ILSERALREGLVVDWMCRNNYLKDQLSFQGSIRERTVIHQAKRFGVNSKRSKSVSEFALTFYDQTKGVLHNDNGGGRDLL
WAAAKLHACGKQINISAYHKHSWYLIKNGELLGYSQSEHLMVAAIARYHRKSFPKKRHESWQLLVDESQRSLVADMSLLL
RLSCSLDRRPEPLVSKIVIEANNKKVNIELIPNNLGQNLDLEKWSLTKAILLVKKIMDVDINIL
>Mature_544_residues
MLGDSINNSSERNSIELDLGEKQDAELCRVATIDIGTNSTHLLIAKIERKLNTFSIELAEKSTTRLGERDPQTGELTSLA
MNRAFSTLKRFKDLSESYKVESLIIAATSAVREAPNGKIFIAEIKKKIGLDVELISGAEEARLIYLGVLSGMQFGNKPHL
VLDIGGGSTELILADSSEARALTSTKIGAVRLQREFIKKDPISSQNELFLRSFIRGSMESAIDKVSKRIEAGEIPVLVAT
SGTAMAIGSLLSNKENHMQSKLQGYKITKNNLDIIVSQLIKMTPSERSQLSSLSERRSEIIVPGALILQTIMNMVDVNEI
ILSERALREGLVVDWMCRNNYLKDQLSFQGSIRERTVIHQAKRFGVNSKRSKSVSEFALTFYDQTKGVLHNDNGGGRDLL
WAAAKLHACGKQINISAYHKHSWYLIKNGELLGYSQSEHLMVAAIARYHRKSFPKKRHESWQLLVDESQRSLVADMSLLL
RLSCSLDRRPEPLVSKIVIEANNKKVNIELIPNNLGQNLDLEKWSLTKAILLVKKIMDVDINIL

Specific function: Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate [H]

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family [H]

Homologues:

Organism=Escherichia coli, GI1788848, Length=435, Percent_Identity=29.1954022988506, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI48994966, Length=506, Percent_Identity=26.2845849802372, Blast_Score=142, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022371
- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.11 [H]

Molecular weight: Translated: 60678; Mature: 60678

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGDSINNSSERNSIELDLGEKQDAELCRVATIDIGTNSTHLLIAKIERKLNTFSIELAE
CCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCEEEEEEHHHCCCEEEEEECC
KSTTRLGERDPQTGELTSLAMNRAFSTLKRFKDLSESYKVESLIIAATSAVREAPNGKIF
CHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHCCCCCEE
IAEIKKKIGLDVELISGAEEARLIYLGVLSGMQFGNKPHLVLDIGGGSTELILADSSEAR
EEEHHHHHCCCEEEECCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCC
ALTSTKIGAVRLQREFIKKDPISSQNELFLRSFIRGSMESAIDKVSKRIEAGEIPVLVAT
CHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
SGTAMAIGSLLSNKENHMQSKLQGYKITKNNLDIIVSQLIKMTPSERSQLSSLSERRSEI
CCHHHHHHHHHCCCHHHHHHHHCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCE
IVPGALILQTIMNMVDVNEIILSERALREGLVVDWMCRNNYLKDQLSFQGSIRERTVIHQ
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCHHHHHHHHH
AKRFGVNSKRSKSVSEFALTFYDQTKGVLHNDNGGGRDLLWAAAKLHACGKQINISAYHK
HHHCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHCCCEEEEEEEEC
HSWYLIKNGELLGYSQSEHLMVAAIARYHRKSFPKKRHESWQLLVDESQRSLVADMSLLL
CEEEEEECCEEECCCCCCCHHHHHHHHHHHHCCCHHHCCCCEEEECCHHHHHHHHHHHHH
RLSCSLDRRPEPLVSKIVIEANNKKVNIELIPNNLGQNLDLEKWSLTKAILLVKKIMDVD
HHHCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
INIL
CCCC
>Mature Secondary Structure
MLGDSINNSSERNSIELDLGEKQDAELCRVATIDIGTNSTHLLIAKIERKLNTFSIELAE
CCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCEEEEEEHHHCCCEEEEEECC
KSTTRLGERDPQTGELTSLAMNRAFSTLKRFKDLSESYKVESLIIAATSAVREAPNGKIF
CHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHCCCCCEE
IAEIKKKIGLDVELISGAEEARLIYLGVLSGMQFGNKPHLVLDIGGGSTELILADSSEAR
EEEHHHHHCCCEEEECCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCC
ALTSTKIGAVRLQREFIKKDPISSQNELFLRSFIRGSMESAIDKVSKRIEAGEIPVLVAT
CHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEC
SGTAMAIGSLLSNKENHMQSKLQGYKITKNNLDIIVSQLIKMTPSERSQLSSLSERRSEI
CCHHHHHHHHHCCCHHHHHHHHCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCE
IVPGALILQTIMNMVDVNEIILSERALREGLVVDWMCRNNYLKDQLSFQGSIRERTVIHQ
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCHHHHHHHHH
AKRFGVNSKRSKSVSEFALTFYDQTKGVLHNDNGGGRDLLWAAAKLHACGKQINISAYHK
HHHCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHCCCEEEEEEEEC
HSWYLIKNGELLGYSQSEHLMVAAIARYHRKSFPKKRHESWQLLVDESQRSLVADMSLLL
CEEEEEECCEEECCCCCCCHHHHHHHHHHHHCCCHHHCCCCEEEECCHHHHHHHHHHHHH
RLSCSLDRRPEPLVSKIVIEANNKKVNIELIPNNLGQNLDLEKWSLTKAILLVKKIMDVD
HHHCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
INIL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]