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The map label for this gene is ispD [H]

Identifier: 124025220

GI number: 124025220

Start: 464000

End: 464671

Strand: Direct

Name: ispD [H]

Synonym: NATL1_05091

Alternate gene names: 124025220

Gene position: 464000-464671 (Clockwise)

Preceding gene: 124025218

Following gene: 124025223

Centisome position: 24.88

GC content: 40.03

Gene sequence:

>672_bases
TTGTTAATTGCGGCGGCAGGAAGTGGGAGTCGAATGGGTGCTGATAGAAATAAACTCCTTTTGAAGGTTGCAGGTAAGAC
GGTTTTAGAATGGACTTTAAAAGCAGCATTCGAGGCTAAAACAATTTCTTGGATTGGAATTATTGGACAACCCAAAGATA
AAAATCCCATTTGTTCAATTCTTGATAACTCAGTAAAGGCAGTTCAATGGATCAATGGAGGATCAACAAGACAGCAGTCA
GTTCAACTGGGATTAGCTGCCCTTCCAAATGATGCAAAGTCTGTGCTGATTCATGATGGAGCAAGATGCTTGGTGAGACC
ATTCGTTTTTGATGAGATCTCAAAGATTGTTTCCAAGGGACAATCTGTTATTGCTGCTTCACAAGTCACTGACACCATAA
AAAAGGTTGATATAGATGGTGAGATTATTGAGAGCCCTCCTCGCTCAGATTTATGGGCTGCGCAGACACCTCAGGGTTTT
CCAGTAAATAAACTGAAGCATGCTCATAGTGAAGCAATCTCCAAAGGATGGAATGTAACCGATGATGCATCCTTGTTTGA
ACGTTTAGGATTGACAGTGAAAATATATGATGCGGGACCTTCAAATATTAAAGTGACAACTCCATTTGATCTCGTAATAG
CAGAGTCTTTATTGTCTACTTTAAAAGACTAA

Upstream 100 bases:

>100_bases
TGCTTTTTATAAACACTTTAAGCTCAGACAGTAAAAATAGTTATTTTTACTGGTCGAATATATTTAATAAATAATCAATG
AAGGGGGATCAGTTTTGCAT

Downstream 100 bases:

>100_bases
GGCTCCCTTTGTCGCCATTCAAGATGGCTTGACTACCTAGTGGAAGTGATGCATTCCCACAGCAGTGTCCAATAGGCAGA
CTTGAAACGATTGGGATTTT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MLIAAAGSGSRMGADRNKLLLKVAGKTVLEWTLKAAFEAKTISWIGIIGQPKDKNPICSILDNSVKAVQWINGGSTRQQS
VQLGLAALPNDAKSVLIHDGARCLVRPFVFDEISKIVSKGQSVIAASQVTDTIKKVDIDGEIIESPPRSDLWAAQTPQGF
PVNKLKHAHSEAISKGWNVTDDASLFERLGLTVKIYDAGPSNIKVTTPFDLVIAESLLSTLKD

Sequences:

>Translated_223_residues
MLIAAAGSGSRMGADRNKLLLKVAGKTVLEWTLKAAFEAKTISWIGIIGQPKDKNPICSILDNSVKAVQWINGGSTRQQS
VQLGLAALPNDAKSVLIHDGARCLVRPFVFDEISKIVSKGQSVIAASQVTDTIKKVDIDGEIIESPPRSDLWAAQTPQGF
PVNKLKHAHSEAISKGWNVTDDASLFERLGLTVKIYDAGPSNIKVTTPFDLVIAESLLSTLKD
>Mature_223_residues
MLIAAAGSGSRMGADRNKLLLKVAGKTVLEWTLKAAFEAKTISWIGIIGQPKDKNPICSILDNSVKAVQWINGGSTRQQS
VQLGLAALPNDAKSVLIHDGARCLVRPFVFDEISKIVSKGQSVIAASQVTDTIKKVDIDGEIIESPPRSDLWAAQTPQGF
PVNKLKHAHSEAISKGWNVTDDASLFERLGLTVKIYDAGPSNIKVTTPFDLVIAESLLSTLKD

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=224, Percent_Identity=32.1428571428571, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 23957; Mature: 23957

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIAAAGSGSRMGADRNKLLLKVAGKTVLEWTLKAAFEAKTISWIGIIGQPKDKNPICSI
CEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCEEEEEEEECCCCCCCCHHHH
LDNSVKAVQWINGGSTRQQSVQLGLAALPNDAKSVLIHDGARCLVRPFVFDEISKIVSKG
HCCCHHEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCC
QSVIAASQVTDTIKKVDIDGEIIESPPRSDLWAAQTPQGFPVNKLKHAHSEAISKGWNVT
CCEEHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
DDASLFERLGLTVKIYDAGPSNIKVTTPFDLVIAESLLSTLKD
CHHHHHHHHCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLIAAAGSGSRMGADRNKLLLKVAGKTVLEWTLKAAFEAKTISWIGIIGQPKDKNPICSI
CEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCEEEEEEEECCCCCCCCHHHH
LDNSVKAVQWINGGSTRQQSVQLGLAALPNDAKSVLIHDGARCLVRPFVFDEISKIVSKG
HCCCHHEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCC
QSVIAASQVTDTIKKVDIDGEIIESPPRSDLWAAQTPQGFPVNKLKHAHSEAISKGWNVT
CCEEHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
DDASLFERLGLTVKIYDAGPSNIKVTTPFDLVIAESLLSTLKD
CHHHHHHHHCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA