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The map label for this gene is murE

Identifier: 124025177

GI number: 124025177

Start: 425023

End: 426552

Strand: Direct

Name: murE

Synonym: NATL1_04641

Alternate gene names: 124025177

Gene position: 425023-426552 (Clockwise)

Preceding gene: 124025176

Following gene: 124025179

Centisome position: 22.79

GC content: 36.6

Gene sequence:

>1530_bases
TTGTCGCGTTATCTGCACACTTTGTTGAAAGCAATTGATCTTCAGGTCCGTTCAGGGTTAGCAAATCCAGAAATAAAAAA
TCTGTCCACTGATTCTAGAGAAATTGAAAAAGGTGATTTGTTCTTAGGTTTAGACGGTGAAAAAGTTGATGGGGGGACCT
TTTGGGCAAAAGCAATTGAGAGAGGTGCTTGTGCAGCCATTATTAGTAAAAAGGCTTCTCTTTTAAATCCGCCAACTAAC
GAAGACCCCGTAGTTATTCTCCCAGAGCCTGTATCACTATTTATGGGTAAATTAGCTGCAGATTTTTGGGGTAAGCCATC
TAGTGAGATTTGTTTGATAGGTATTACTGGTACTAACGGAAAAACGACTACATCATTTTTAATTGAATTTCTGACTACTT
CGCTTGGCCATCCATCCGCTTTGTTTGGAACATTAATTAATAGATGGCCTAACTATGAGGAAACTTCAAAATATACAACT
ACTTTTGCGGTCCCTTTGCAGGCAAAACTTAGGAAAGCCGTTCAGGCAGGAGTTGAATATGGAGCAATGGAAGTAAGTTC
ACATGCGTTGTCTCAGAATAGAGTCGCTGGTTGTGATTTCAATGGGGCAATATTTACAAACCTTTCAAGAGACCATTTAG
ATTATCACGATTCAATGGAATCTTATTTTGAGGCTAAAGCTAGTTTGTTCCGATCACATCTGATAGACGATGATGGTCCT
AGATCAGTAATTAATATAGACGATAAATGGGGTTCAATATTAGCAAAAGAGCTCAACAAAAAATGTTGGACATGTTCATT
AAAAGAGAACTCCCAAATCAGAGAGAAACCAGATTTATATATTAGCAATCTTCAAATTATGCAAGACGGATACATGGGAA
AATTGCATACGCCATTTGGGGTGGGAAATTTTATTTCACCACTAATAGGTGAGTTTAATTTAATGAATATGTTGCAAGCA
ATAGGAATTTTAGTTCAAAGGGGACTCCCATTAAATGATCTTTTAGAAGCTTTAAACAAGTTCCCTGGAGTGCCAGGGAG
AATGCAACTGATAAACATGGATGGATTTAAAGTAAAAGACGGCTATCCACTAGTGATAGTAGATTATGCTCATACACCAG
ATGGTTTACAAAATGCTTTAATCGCATCGAGATCATTGACGAAAAAAAGATTAATTTGTGTCTTTGGTTGTGGTGGTGAT
AGAGATAAAGGTAAAAGATCTAAGATGGGGGAAGTTGCTGCTAAGTTTGCAGATTATATAGTTGTGACATCTGATAATCC
TCGACAAGAAGACCCAATCGAAATTATTAAAGATATTCAAAAGGGTATAACAATTGATTCTGAAATTTCTGTTGAGCCAG
AGAGATCCATTGCAATTCAATTTGCCATAGCAAAAGCCAAGAAAAATGATGTTGTTTTAATCGCAGGGAAAGGTCATGAG
GATTATCAAATTCTAAAAGATCAGACAATTTATTTTGACGATCGTGAACAAGCTAGAAAAGCACTATCTTTAAGAACAGA
TGTTATTTGA

Upstream 100 bases:

>100_bases
TTACTGAATCGTAAGTAGATTTCTTTTGTGGTGAAAATCATGCAGATCTTTTTATACTTGAAATGATCCTTCATTGATTT
TTCATTATTCGAGGTTTGTT

Downstream 100 bases:

>100_bases
CTACTTAGTCGAGATGAAATTTTTTAGACCAATAACAAGGTTATCTATTTCGTTTGAATCTGTAGTTATGTGAACGCAAG
CACGAAGCCATTTAGGATCC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MSRYLHTLLKAIDLQVRSGLANPEIKNLSTDSREIEKGDLFLGLDGEKVDGGTFWAKAIERGACAAIISKKASLLNPPTN
EDPVVILPEPVSLFMGKLAADFWGKPSSEICLIGITGTNGKTTTSFLIEFLTTSLGHPSALFGTLINRWPNYEETSKYTT
TFAVPLQAKLRKAVQAGVEYGAMEVSSHALSQNRVAGCDFNGAIFTNLSRDHLDYHDSMESYFEAKASLFRSHLIDDDGP
RSVINIDDKWGSILAKELNKKCWTCSLKENSQIREKPDLYISNLQIMQDGYMGKLHTPFGVGNFISPLIGEFNLMNMLQA
IGILVQRGLPLNDLLEALNKFPGVPGRMQLINMDGFKVKDGYPLVIVDYAHTPDGLQNALIASRSLTKKRLICVFGCGGD
RDKGKRSKMGEVAAKFADYIVVTSDNPRQEDPIEIIKDIQKGITIDSEISVEPERSIAIQFAIAKAKKNDVVLIAGKGHE
DYQILKDQTIYFDDREQARKALSLRTDVI

Sequences:

>Translated_509_residues
MSRYLHTLLKAIDLQVRSGLANPEIKNLSTDSREIEKGDLFLGLDGEKVDGGTFWAKAIERGACAAIISKKASLLNPPTN
EDPVVILPEPVSLFMGKLAADFWGKPSSEICLIGITGTNGKTTTSFLIEFLTTSLGHPSALFGTLINRWPNYEETSKYTT
TFAVPLQAKLRKAVQAGVEYGAMEVSSHALSQNRVAGCDFNGAIFTNLSRDHLDYHDSMESYFEAKASLFRSHLIDDDGP
RSVINIDDKWGSILAKELNKKCWTCSLKENSQIREKPDLYISNLQIMQDGYMGKLHTPFGVGNFISPLIGEFNLMNMLQA
IGILVQRGLPLNDLLEALNKFPGVPGRMQLINMDGFKVKDGYPLVIVDYAHTPDGLQNALIASRSLTKKRLICVFGCGGD
RDKGKRSKMGEVAAKFADYIVVTSDNPRQEDPIEIIKDIQKGITIDSEISVEPERSIAIQFAIAKAKKNDVVLIAGKGHE
DYQILKDQTIYFDDREQARKALSLRTDVI
>Mature_508_residues
SRYLHTLLKAIDLQVRSGLANPEIKNLSTDSREIEKGDLFLGLDGEKVDGGTFWAKAIERGACAAIISKKASLLNPPTNE
DPVVILPEPVSLFMGKLAADFWGKPSSEICLIGITGTNGKTTTSFLIEFLTTSLGHPSALFGTLINRWPNYEETSKYTTT
FAVPLQAKLRKAVQAGVEYGAMEVSSHALSQNRVAGCDFNGAIFTNLSRDHLDYHDSMESYFEAKASLFRSHLIDDDGPR
SVINIDDKWGSILAKELNKKCWTCSLKENSQIREKPDLYISNLQIMQDGYMGKLHTPFGVGNFISPLIGEFNLMNMLQAI
GILVQRGLPLNDLLEALNKFPGVPGRMQLINMDGFKVKDGYPLVIVDYAHTPDGLQNALIASRSLTKKRLICVFGCGGDR
DKGKRSKMGEVAAKFADYIVVTSDNPRQEDPIEIIKDIQKGITIDSEISVEPERSIAIQFAIAKAKKNDVVLIAGKGHED
YQILKDQTIYFDDREQARKALSLRTDVI

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=475, Percent_Identity=40.2105263157895, Blast_Score=280, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_PROM1 (A2C0L8)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014293.1
- ProteinModelPortal:   A2C0L8
- SMR:   A2C0L8
- STRING:   A2C0L8
- GeneID:   4780153
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_04641
- eggNOG:   COG0769
- HOGENOM:   HBG602753
- OMA:   VLGCGGD
- ProtClustDB:   PRK00139
- BioCyc:   PMAR167555:NATL1_04641-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 56281; Mature: 56150

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: BINDING 32-32 BINDING 186-186 BINDING 192-192 BINDING 194-194 BINDING 401-401 BINDING 476-476 BINDING 480-480

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYLHTLLKAIDLQVRSGLANPEIKNLSTDSREIEKGDLFLGLDGEKVDGGTFWAKAIE
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHH
RGACAAIISKKASLLNPPTNEDPVVILPEPVSLFMGKLAADFWGKPSSEICLIGITGTNG
CCHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
KTTTSFLIEFLTTSLGHPSALFGTLINRWPNYEETSKYTTTFAVPLQAKLRKAVQAGVEY
CHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHH
GAMEVSSHALSQNRVAGCDFNGAIFTNLSRDHLDYHDSMESYFEAKASLFRSHLIDDDGP
CHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCC
RSVINIDDKWGSILAKELNKKCWTCSLKENSQIREKPDLYISNLQIMQDGYMGKLHTPFG
CCEEEECCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEECEEEECCCCCCCCCCCCC
VGNFISPLIGEFNLMNMLQAIGILVQRGLPLNDLLEALNKFPGVPGRMQLINMDGFKVKD
CHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEECC
GYPLVIVDYAHTPDGLQNALIASRSLTKKRLICVFGCGGDRDKGKRSKMGEVAAKFADYI
CCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCEE
VVTSDNPRQEDPIEIIKDIQKGITIDSEISVEPERSIAIQFAIAKAKKNDVVLIAGKGHE
EEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCC
DYQILKDQTIYFDDREQARKALSLRTDVI
CHHEECCCEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRYLHTLLKAIDLQVRSGLANPEIKNLSTDSREIEKGDLFLGLDGEKVDGGTFWAKAIE
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHH
RGACAAIISKKASLLNPPTNEDPVVILPEPVSLFMGKLAADFWGKPSSEICLIGITGTNG
CCHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
KTTTSFLIEFLTTSLGHPSALFGTLINRWPNYEETSKYTTTFAVPLQAKLRKAVQAGVEY
CHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHH
GAMEVSSHALSQNRVAGCDFNGAIFTNLSRDHLDYHDSMESYFEAKASLFRSHLIDDDGP
CHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCC
RSVINIDDKWGSILAKELNKKCWTCSLKENSQIREKPDLYISNLQIMQDGYMGKLHTPFG
CCEEEECCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEECEEEECCCCCCCCCCCCC
VGNFISPLIGEFNLMNMLQAIGILVQRGLPLNDLLEALNKFPGVPGRMQLINMDGFKVKD
CHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEECC
GYPLVIVDYAHTPDGLQNALIASRSLTKKRLICVFGCGGDRDKGKRSKMGEVAAKFADYI
CCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCEE
VVTSDNPRQEDPIEIIKDIQKGITIDSEISVEPERSIAIQFAIAKAKKNDVVLIAGKGHE
EEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCC
DYQILKDQTIYFDDREQARKALSLRTDVI
CHHEECCCEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA