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The map label for this gene is lipB

Identifier: 124025166

GI number: 124025166

Start: 413771

End: 414505

Strand: Direct

Name: lipB

Synonym: NATL1_04531

Alternate gene names: 124025166

Gene position: 413771-414505 (Clockwise)

Preceding gene: 124025161

Following gene: 124025167

Centisome position: 22.19

GC content: 37.96

Gene sequence:

>735_bases
ATGGATAAGAAGCTTCATTCTGTTTCGCCTGAGTCAGGTCCTAATAGCAACTTAGATCTAACCCCTCAACTTGCACAATC
TTCTTCCGCTTTTCTTTTTGAACCTAAAAATATTGTGCCATTTGAGACTGCCTTAGGTTGGCAGAAGAATTTTCTAAAAA
ACCTTATTGAGGAACCGTTCTCACCCCAAGCAGTATGGCTTCTTGAACATTTTTCTTGTTTCACAATGGGTAGAGGTAGC
GATAAAAAAAATTTGTTGTTCGAAGAAAATAATTCTCCTTTGCCAGTTTTTAGTATTGAGAGAGGAGGTGAAGTTACGCA
TCACATGCCTGGGCAGATCGTTGGATATCTGGTTCTAAATTTAAGTCTTCACAAAAAAGACTTGGCTTGGTATTTGAGAG
AATTGGAGCAAGTGCTGATCGATGTTCTTGACTTGTTGGGAATAGAGGGGAAGAGGGTAGATGGCTTAACAGGAGTTTGG
TGTGAAGATAAAAAGGTTGGTTCAATAGGAATTGGTTGCAAGAGATGGGTGACTCAGCATGGATTTTCACTAAATGTAGA
TTGTGATCTAATTGGTTTTGAAAAGATAATTCCTTGTGGACTAGATAAAGTGAAAGTAGGGAAATTGAGTGATTGGATAC
CTGGCATCAAAGTCTGTGACGTTACGCCTCTTTTAAGGGAATCTGTTAAAAGACGTTTTAAATTGAACTGGGAAAAAATA
AATCAATCTCTTTAA

Upstream 100 bases:

>100_bases
CATCAGCTTCTTGCACCATTTCTTGGAAATTGTGAGTTGCTTTATCAATTTTTGTTTTGGTGTAATCACGAAGTGATGGA
GTTAATTCAAGATTGCGTCC

Downstream 100 bases:

>100_bases
ATATCACTAAAAAAATAACAAATTATTTTTTGGCTATGACAAAAACTAACTCACAAAAAAATATATTTTCTTCTGAGGAT
GCTTTGGCTTTTTGGATCCC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MDKKLHSVSPESGPNSNLDLTPQLAQSSSAFLFEPKNIVPFETALGWQKNFLKNLIEEPFSPQAVWLLEHFSCFTMGRGS
DKKNLLFEENNSPLPVFSIERGGEVTHHMPGQIVGYLVLNLSLHKKDLAWYLRELEQVLIDVLDLLGIEGKRVDGLTGVW
CEDKKVGSIGIGCKRWVTQHGFSLNVDCDLIGFEKIIPCGLDKVKVGKLSDWIPGIKVCDVTPLLRESVKRRFKLNWEKI
NQSL

Sequences:

>Translated_244_residues
MDKKLHSVSPESGPNSNLDLTPQLAQSSSAFLFEPKNIVPFETALGWQKNFLKNLIEEPFSPQAVWLLEHFSCFTMGRGS
DKKNLLFEENNSPLPVFSIERGGEVTHHMPGQIVGYLVLNLSLHKKDLAWYLRELEQVLIDVLDLLGIEGKRVDGLTGVW
CEDKKVGSIGIGCKRWVTQHGFSLNVDCDLIGFEKIIPCGLDKVKVGKLSDWIPGIKVCDVTPLLRESVKRRFKLNWEKI
NQSL
>Mature_244_residues
MDKKLHSVSPESGPNSNLDLTPQLAQSSSAFLFEPKNIVPFETALGWQKNFLKNLIEEPFSPQAVWLLEHFSCFTMGRGS
DKKNLLFEENNSPLPVFSIERGGEVTHHMPGQIVGYLVLNLSLHKKDLAWYLRELEQVLIDVLDLLGIEGKRVDGLTGVW
CEDKKVGSIGIGCKRWVTQHGFSLNVDCDLIGFEKIIPCGLDKVKVGKLSDWIPGIKVCDVTPLLRESVKRRFKLNWEKI
NQSL

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=188, Percent_Identity=34.5744680851064, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI87081767, Length=165, Percent_Identity=35.7575757575758, Blast_Score=115, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21355483, Length=146, Percent_Identity=34.9315068493151, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_PROM1 (A2C0K7)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014282.1
- ProteinModelPortal:   A2C0K7
- SMR:   A2C0K7
- STRING:   A2C0K7
- GeneID:   4779293
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_04531
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   THHLPGQ
- ProtClustDB:   PRK14344
- BioCyc:   PMAR167555:NATL1_04531-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 27512; Mature: 27512

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKLHSVSPESGPNSNLDLTPQLAQSSSAFLFEPKNIVPFETALGWQKNFLKNLIEEPF
CCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SPQAVWLLEHFSCFTMGRGSDKKNLLFEENNSPLPVFSIERGGEVTHHMPGQIVGYLVLN
CCCEEHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHH
LSLHKKDLAWYLRELEQVLIDVLDLLGIEGKRVDGLTGVWCEDKKVGSIGIGCKRWVTQH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHC
GFSLNVDCDLIGFEKIIPCGLDKVKVGKLSDWIPGIKVCDVTPLLRESVKRRFKLNWEKI
CCEEECCEEEECHHHHCCCCCCCEECCCHHHCCCCCEEECCCHHHHHHHHHHHCCCHHHH
NQSL
CCCC
>Mature Secondary Structure
MDKKLHSVSPESGPNSNLDLTPQLAQSSSAFLFEPKNIVPFETALGWQKNFLKNLIEEPF
CCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SPQAVWLLEHFSCFTMGRGSDKKNLLFEENNSPLPVFSIERGGEVTHHMPGQIVGYLVLN
CCCEEHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEECCCCCCCCCCCHHHHHHHHHH
LSLHKKDLAWYLRELEQVLIDVLDLLGIEGKRVDGLTGVWCEDKKVGSIGIGCKRWVTQH
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHC
GFSLNVDCDLIGFEKIIPCGLDKVKVGKLSDWIPGIKVCDVTPLLRESVKRRFKLNWEKI
CCEEECCEEEECHHHHCCCCCCCEECCCHHHCCCCCEEECCCHHHHHHHHHHHCCCHHHH
NQSL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA