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The map label for this gene is pyrB

Identifier: 124025025

GI number: 124025025

Start: 289712

End: 290731

Strand: Reverse

Name: pyrB

Synonym: NATL1_03121

Alternate gene names: 124025025

Gene position: 290731-289712 (Counterclockwise)

Preceding gene: 124025026

Following gene: 124025023

Centisome position: 15.59

GC content: 34.41

Gene sequence:

>1020_bases
ATGAATAATTGGAAACATAATCACGTTCTTGATTTATCTACGTTTTCTTTAGAGGATTACAAAACAGTTTTAGAATTAAC
AACAAGATTTAAAGATGTCCATAAATCAAGTTCTAAGAAACTTCCCGCTCTTCATGGAAGATTAATTGCCAACTTATTTT
TCGAGCCAAGTACAAGGACAAGAACTAGCTTTGAGCTTGCAGCAAAGAGACTATCTGCGGATGTTCAGAATTTTTCTGTA
TCTTCAAGTTCTCTAAGCAAAGGAGAGACTCCTTTAGACACTATTCTCACATACATCTCAATGGGGGCTGATATCTTAGT
AATTAGGCATGAGTCAACAAATGTACCCGCAGAGTTAGCTAATTACGTTGACATAAATAACATCAATACATCCATTCTCA
ATGCAGGTGATGGATTTCATAGCCATCCAAGTCAAGGTCTTTTGGATCTATTCACACTTGCTACTTTTTTTAATCCAAAT
GAACCATCAACTAATAGTCTTTTAAATAAAAAAATTACAATAGTTGGAGATATTCTTCATTCTAGAGTTGCCAGATCAAA
CCTTTGGGCTCTAACTGCATGTGGAGCAGAGGTAACACTATGTGGACCTCCAAGCCTTCTTCCAGAAGAATTCATTGATT
TTGTTCAGAATCCTCGGCTAGGGCAAAATTTTGATCCCATTAATAAGAGAGGTTCTGTTTTCATAAAAAGATCGCTAAAA
GACGCATTAAAAAATAGTGATGCTGTTATGACACTTCGATTACAGAAAGAGAGAATGAAGCAAAATATGCTTAAGGATCT
TGATAGTTATTATGCACAATATGGGATAACCCATGAAAGTTTAAAATGGTGTGAAAAGAAAGTTCCTGTCCTTCATCCTG
GACCAGTAAATAGAGGGGTAGAAATAAGTAACCGATTAGTTGAAGATAATTCAATCAATCTTATAAGTAAACAAGTAGAA
AATGGTATTCCAACAAGAATGGCTTTGTTGTATTTACTAGGGTTAAATAAAAAAGATTAA

Upstream 100 bases:

>100_bases
GTTGGTACCTTAAAAGTAGTAGAAGTGTTAGCAAAAGGGTTAGAGGTGATCGAATTCCTCCAAAAAATAAGTGTTGGGAA
CCATCAGCATTTGAGGGATT

Downstream 100 bases:

>100_bases
TTATATTTAGAATAGATTTATAAAAGTTTTAAACCTTCTGAAAAAAGTCCATACGCTAATCCTATTCTGAACATATCAAA
AACCCTAACTAGTATTATTG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNNWKHNHVLDLSTFSLEDYKTVLELTTRFKDVHKSSSKKLPALHGRLIANLFFEPSTRTRTSFELAAKRLSADVQNFSV
SSSSLSKGETPLDTILTYISMGADILVIRHESTNVPAELANYVDINNINTSILNAGDGFHSHPSQGLLDLFTLATFFNPN
EPSTNSLLNKKITIVGDILHSRVARSNLWALTACGAEVTLCGPPSLLPEEFIDFVQNPRLGQNFDPINKRGSVFIKRSLK
DALKNSDAVMTLRLQKERMKQNMLKDLDSYYAQYGITHESLKWCEKKVPVLHPGPVNRGVEISNRLVEDNSINLISKQVE
NGIPTRMALLYLLGLNKKD

Sequences:

>Translated_339_residues
MNNWKHNHVLDLSTFSLEDYKTVLELTTRFKDVHKSSSKKLPALHGRLIANLFFEPSTRTRTSFELAAKRLSADVQNFSV
SSSSLSKGETPLDTILTYISMGADILVIRHESTNVPAELANYVDINNINTSILNAGDGFHSHPSQGLLDLFTLATFFNPN
EPSTNSLLNKKITIVGDILHSRVARSNLWALTACGAEVTLCGPPSLLPEEFIDFVQNPRLGQNFDPINKRGSVFIKRSLK
DALKNSDAVMTLRLQKERMKQNMLKDLDSYYAQYGITHESLKWCEKKVPVLHPGPVNRGVEISNRLVEDNSINLISKQVE
NGIPTRMALLYLLGLNKKD
>Mature_339_residues
MNNWKHNHVLDLSTFSLEDYKTVLELTTRFKDVHKSSSKKLPALHGRLIANLFFEPSTRTRTSFELAAKRLSADVQNFSV
SSSSLSKGETPLDTILTYISMGADILVIRHESTNVPAELANYVDINNINTSILNAGDGFHSHPSQGLLDLFTLATFFNPN
EPSTNSLLNKKITIVGDILHSRVARSNLWALTACGAEVTLCGPPSLLPEEFIDFVQNPRLGQNFDPINKRGSVFIKRSLK
DALKNSDAVMTLRLQKERMKQNMLKDLDSYYAQYGITHESLKWCEKKVPVLHPGPVNRGVEISNRLVEDNSINLISKQVE
NGIPTRMALLYLLGLNKKD

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=326, Percent_Identity=30.6748466257669, Blast_Score=131, Evalue=1e-30,
Organism=Escherichia coli, GI2367364, Length=336, Percent_Identity=31.25, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790703, Length=209, Percent_Identity=28.2296650717703, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=327, Percent_Identity=30.8868501529052, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322331, Length=335, Percent_Identity=25.3731343283582, Blast_Score=102, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24642586, Length=330, Percent_Identity=31.2121212121212, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_PROM1 (A2C066)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014141.1
- ProteinModelPortal:   A2C066
- SMR:   A2C066
- STRING:   A2C066
- GeneID:   4780421
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_03121
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   PMAR167555:NATL1_03121-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 37948; Mature: 37948

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNWKHNHVLDLSTFSLEDYKTVLELTTRFKDVHKSSSKKLPALHGRLIANLFFEPSTRT
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC
RTSFELAAKRLSADVQNFSVSSSSLSKGETPLDTILTYISMGADILVIRHESTNVPAELA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHH
NYVDINNINTSILNAGDGFHSHPSQGLLDLFTLATFFNPNEPSTNSLLNKKITIVGDILH
HCEECCCCCHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
SRVARSNLWALTACGAEVTLCGPPSLLPEEFIDFVQNPRLGQNFDPINKRGSVFIKRSLK
HHHHHCCCEEEEECCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHH
DALKNSDAVMTLRLQKERMKQNMLKDLDSYYAQYGITHESLKWCEKKVPVLHPGPVNRGV
HHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEECCCCCCCCC
EISNRLVEDNSINLISKQVENGIPTRMALLYLLGLNKKD
HHHHHEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNNWKHNHVLDLSTFSLEDYKTVLELTTRFKDVHKSSSKKLPALHGRLIANLFFEPSTRT
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCC
RTSFELAAKRLSADVQNFSVSSSSLSKGETPLDTILTYISMGADILVIRHESTNVPAELA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHH
NYVDINNINTSILNAGDGFHSHPSQGLLDLFTLATFFNPNEPSTNSLLNKKITIVGDILH
HCEECCCCCHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
SRVARSNLWALTACGAEVTLCGPPSLLPEEFIDFVQNPRLGQNFDPINKRGSVFIKRSLK
HHHHHCCCEEEEECCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHH
DALKNSDAVMTLRLQKERMKQNMLKDLDSYYAQYGITHESLKWCEKKVPVLHPGPVNRGV
HHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEECCCCCCCCC
EISNRLVEDNSINLISKQVENGIPTRMALLYLLGLNKKD
HHHHHEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA