Click here to switch to the map view.

The map label for this gene is gshB [H]

Identifier: 124024966

GI number: 124024966

Start: 234221

End: 235150

Strand: Reverse

Name: gshB [H]

Synonym: NATL1_02531

Alternate gene names: 124024966

Gene position: 235150-234221 (Counterclockwise)

Preceding gene: 124024967

Following gene: 124024964

Centisome position: 12.61

GC content: 39.35

Gene sequence:

>930_bases
ATGAAACAACTATTTGTTTTAGATCCAATTGAAAATATCAATCCTAAGAAGGATTCATCAGCAGCACTTATGCAAGCCGC
ATCAAGAGCCAACATAGATGTTTGGATCTGTACTCCCTCGGACCTGCAAGCCCGAGGAGACGATGCATGGGTCGTTTCTA
ACAAGGTCAATTGTGAACCATGGATCAATGTCCAGTCACCTCGAAGCCTTCCTTTAAGAGATTTCTCATGCATTTGGATG
CGCAAAGATCCACCTGTTGACGAGGCTTTTTTATACGCCACTCATTTATTAGAAGTTGCAGAAAGAGATGGTGTCAATGT
AATTAACAAGCCTGCATCACTTAGAGCTTGGAATGAAAAGTTAGGAGCTTTAAGATTTAGCGATTTAATGGCTCCCACTC
TTGTCGCAAGTAGGGTGGAACAATTAATTACATTTGCAAAAGAGTATGGAGAAGTTGTATTAAAACCACTTGGAGGGAAA
GGTGGGCAAGGAGTCATACGAATTGCAAAGGATGCTCCAGGCTTAGAAGCATTACTCGAACTGGTTACTTCACAAGAACA
TTTGCCAGTGATGATGCAACAATTCCTACCAGAAGTAATCAATGGTGATAAAAGAATCCTTTTAGTTAATGGAGAGCCAT
TAGGTGCAATTAATAGACGTCCAAAGGAAGGAGACTTCAGAAGCAACTTGGCTTTAGGTGGAAAAGCAGAGACAACTAAA
TTAACTCCTAAAGAAATAGAGATATGTAATCAAATAAAGCCCGCTTTACAAGAGGAAGGTCTCTTTTTTGTTGGAATAGA
TGTGATCGGAGGAATGCTAAGTGAAATTAATGTGACGAGTCCAACTGGTATTAGAGAAGTAGAGAACCTAATGAATGTGC
CATTAGCAGATCAAGTAATTGATTACCTTATAGATCATTTGAACAATTAA

Upstream 100 bases:

>100_bases
AATTTTTATCAATGGAAAAAGTATTGGAGGTTGCGACGAGCTCTACGAACTTGAGAGAAATAACGAATTAAATGAACTGA
TTGGAATTAGAAATTGATTC

Downstream 100 bases:

>100_bases
CTCTATCTAAATTTAATTGATTTCTTAAAACTTCAAATTTCAAAGCTACTTCTTGCATTTCCCCCTCAACAGTTGAACCT
TTTACTAAACCAGCGCCAGC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKQLFVLDPIENINPKKDSSAALMQAASRANIDVWICTPSDLQARGDDAWVVSNKVNCEPWINVQSPRSLPLRDFSCIWM
RKDPPVDEAFLYATHLLEVAERDGVNVINKPASLRAWNEKLGALRFSDLMAPTLVASRVEQLITFAKEYGEVVLKPLGGK
GGQGVIRIAKDAPGLEALLELVTSQEHLPVMMQQFLPEVINGDKRILLVNGEPLGAINRRPKEGDFRSNLALGGKAETTK
LTPKEIEICNQIKPALQEEGLFFVGIDVIGGMLSEINVTSPTGIREVENLMNVPLADQVIDYLIDHLNN

Sequences:

>Translated_309_residues
MKQLFVLDPIENINPKKDSSAALMQAASRANIDVWICTPSDLQARGDDAWVVSNKVNCEPWINVQSPRSLPLRDFSCIWM
RKDPPVDEAFLYATHLLEVAERDGVNVINKPASLRAWNEKLGALRFSDLMAPTLVASRVEQLITFAKEYGEVVLKPLGGK
GGQGVIRIAKDAPGLEALLELVTSQEHLPVMMQQFLPEVINGDKRILLVNGEPLGAINRRPKEGDFRSNLALGGKAETTK
LTPKEIEICNQIKPALQEEGLFFVGIDVIGGMLSEINVTSPTGIREVENLMNVPLADQVIDYLIDHLNN
>Mature_309_residues
MKQLFVLDPIENINPKKDSSAALMQAASRANIDVWICTPSDLQARGDDAWVVSNKVNCEPWINVQSPRSLPLRDFSCIWM
RKDPPVDEAFLYATHLLEVAERDGVNVINKPASLRAWNEKLGALRFSDLMAPTLVASRVEQLITFAKEYGEVVLKPLGGK
GGQGVIRIAKDAPGLEALLELVTSQEHLPVMMQQFLPEVINGDKRILLVNGEPLGAINRRPKEGDFRSNLALGGKAETTK
LTPKEIEICNQIKPALQEEGLFFVGIDVIGGMLSEINVTSPTGIREVENLMNVPLADQVIDYLIDHLNN

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=302, Percent_Identity=43.046357615894, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1787076, Length=159, Percent_Identity=32.7044025157233, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34111; Mature: 34111

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLFVLDPIENINPKKDSSAALMQAASRANIDVWICTPSDLQARGDDAWVVSNKVNCEP
CCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCCCCC
WINVQSPRSLPLRDFSCIWMRKDPPVDEAFLYATHLLEVAERDGVNVINKPASLRAWNEK
CCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHH
LGALRFSDLMAPTLVASRVEQLITFAKEYGEVVLKPLGGKGGQGVIRIAKDAPGLEALLE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEECCCCCHHHHHH
LVTSQEHLPVMMQQFLPEVINGDKRILLVNGEPLGAINRRPKEGDFRSNLALGGKAETTK
HHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCC
LTPKEIEICNQIKPALQEEGLFFVGIDVIGGMLSEINVTSPTGIREVENLMNVPLADQVI
CCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHH
DYLIDHLNN
HHHHHHHCC
>Mature Secondary Structure
MKQLFVLDPIENINPKKDSSAALMQAASRANIDVWICTPSDLQARGDDAWVVSNKVNCEP
CCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCCCCC
WINVQSPRSLPLRDFSCIWMRKDPPVDEAFLYATHLLEVAERDGVNVINKPASLRAWNEK
CCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHH
LGALRFSDLMAPTLVASRVEQLITFAKEYGEVVLKPLGGKGGQGVIRIAKDAPGLEALLE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEECCCCCHHHHHH
LVTSQEHLPVMMQQFLPEVINGDKRILLVNGEPLGAINRRPKEGDFRSNLALGGKAETTK
HHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCC
LTPKEIEICNQIKPALQEEGLFFVGIDVIGGMLSEINVTSPTGIREVENLMNVPLADQVI
CCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHH
DYLIDHLNN
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917486 [H]