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The map label for this gene is menF [H]

Identifier: 124024965

GI number: 124024965

Start: 232843

End: 234243

Strand: Direct

Name: menF [H]

Synonym: NATL1_02521

Alternate gene names: 124024965

Gene position: 232843-234243 (Clockwise)

Preceding gene: 124024961

Following gene: 124024968

Centisome position: 12.49

GC content: 34.33

Gene sequence:

>1401_bases
ATGAATTTAGAACCCGTCTTTAGTGAGCTTTTAGTTAGTTCTCTTCAGAAATGGAGTGTAAGAAAAGTTGATGAATGTAT
CTTGAGCATTTCTGTTCCTGTAAGTCAAACAGATCCTTTAACGACATTACCTTTAATTGCTGAAAAACATCAGTTTAGAT
TTCTTTGGGATCTCTCTCCCGGATTGTGCCTTTCTGCCGGAGGTCATTGTCAATCTTTGGATTTATCTGGACCAAAGAGG
TTTGAAAATGCACAAAGATTTAGTGATGAGATTTTTACTCGATTAATAGAAATTTCTCCAAGTCCAGCATTTTCAGCCTC
AAGGATTTTATTTTCATTTTCTTTCTTTGATCAAATTAAAAGTAATGAAAAATCAATAGATGATAAATTTTCCCTACAGG
CTGTTTTGCCTAAGTGGCAATTAACCGCAAAAGATGGATTAACATGGTTACGTTTAAATTCAGTTGCCCAAAATCCTTCA
GATGTTCGTGAAGCCATAGAAAGATTATGGTCAATTCGAGAAAAGATAAATAAATCACCAGTCCGAATTGTCAATGATGA
AAAAGAAAATTTCTTAGTCGATAATTTCTCAGATGATTGGAAATCGCAATATCGAGATGCGTTGGCTAAAGGGATCGAAT
TAATAAATGCAGGTGATTTGGATAAACTTGTATTGGCTACGAGACAACATCTCTCCCTAAGAAAACCATTAGATCCACTG
CATTTACTTGCTCGTTTAAGAGTTCAGCAAACTAATAGTTGTCGATTCCTATGGCAAAAAAATCATGATGAGTCATTTTT
TGGTGCATCGCCTGAAAGATTGATAAGTCTCAATCAAAATCAGTTATTAATCGATGCTTTAGCTGGTACTGCAAAAAAAG
GTGATGATGGGAGAGAATTGCTTGCTTCCTCTAAAGATTTAAGAGAACACCATTTTGTAGTTAATTCTATTGTCGAACAA
CTTTTAAAAAGAGGTATTAAAGCAAGTTATCCATCTCAACCAAAATTAATGACTCAAGACCATTTAATTCATTTGCATAC
TCTTATTCAAGCCTCTGTTAAAGAGAAATCTCCTCTTGATTTAGTTGAAGCACTTCATCCAACCCCTGCTGTTGCAGGAT
TACCTCTGAATAAATCTTTGAGTTGGTTGAGAGCTTTAGAACCATTTGACCGTAAAACATATGCTTCTCCAATTGGGTGG
ATAGATAAAAATCAAAATTCAGAATTTAGGGTTGCCATACGTTATGGACAGCTTAAAGCTAATGAACTTAAATTATTTGC
TGGCGCTGGTTTAGTAAAAGGTTCAACTGTTGAGGGGGAAATGCAAGAAGTAGCTTTGAAATTTGAAGTTTTAAGAAATC
AATTAAATTTAGATAGAGTTAATTGTTCAAATGATCTATAA

Upstream 100 bases:

>100_bases
CTGATAAACTAATCCTTCCTGTTTGTTTCTTGGTGTATCCCTTAAGACTAGGCAGCAGTTCATGCATTGGTTTATTTATT
TATAAAGGGTCTAATTGGTT

Downstream 100 bases:

>100_bases
GGTAATCAATTACTTGATCTGCTAATGGCACATTCATTAGGTTCTCTACTTCTCTAATACCAGTTGGACTCGTCACATTA
ATTTCACTTAGCATTCCTCC

Product: isochorismate synthase

Products: NA

Alternate protein names: Amonabactin; Isochorismate mutase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MNLEPVFSELLVSSLQKWSVRKVDECILSISVPVSQTDPLTTLPLIAEKHQFRFLWDLSPGLCLSAGGHCQSLDLSGPKR
FENAQRFSDEIFTRLIEISPSPAFSASRILFSFSFFDQIKSNEKSIDDKFSLQAVLPKWQLTAKDGLTWLRLNSVAQNPS
DVREAIERLWSIREKINKSPVRIVNDEKENFLVDNFSDDWKSQYRDALAKGIELINAGDLDKLVLATRQHLSLRKPLDPL
HLLARLRVQQTNSCRFLWQKNHDESFFGASPERLISLNQNQLLIDALAGTAKKGDDGRELLASSKDLREHHFVVNSIVEQ
LLKRGIKASYPSQPKLMTQDHLIHLHTLIQASVKEKSPLDLVEALHPTPAVAGLPLNKSLSWLRALEPFDRKTYASPIGW
IDKNQNSEFRVAIRYGQLKANELKLFAGAGLVKGSTVEGEMQEVALKFEVLRNQLNLDRVNCSNDL

Sequences:

>Translated_466_residues
MNLEPVFSELLVSSLQKWSVRKVDECILSISVPVSQTDPLTTLPLIAEKHQFRFLWDLSPGLCLSAGGHCQSLDLSGPKR
FENAQRFSDEIFTRLIEISPSPAFSASRILFSFSFFDQIKSNEKSIDDKFSLQAVLPKWQLTAKDGLTWLRLNSVAQNPS
DVREAIERLWSIREKINKSPVRIVNDEKENFLVDNFSDDWKSQYRDALAKGIELINAGDLDKLVLATRQHLSLRKPLDPL
HLLARLRVQQTNSCRFLWQKNHDESFFGASPERLISLNQNQLLIDALAGTAKKGDDGRELLASSKDLREHHFVVNSIVEQ
LLKRGIKASYPSQPKLMTQDHLIHLHTLIQASVKEKSPLDLVEALHPTPAVAGLPLNKSLSWLRALEPFDRKTYASPIGW
IDKNQNSEFRVAIRYGQLKANELKLFAGAGLVKGSTVEGEMQEVALKFEVLRNQLNLDRVNCSNDL
>Mature_466_residues
MNLEPVFSELLVSSLQKWSVRKVDECILSISVPVSQTDPLTTLPLIAEKHQFRFLWDLSPGLCLSAGGHCQSLDLSGPKR
FENAQRFSDEIFTRLIEISPSPAFSASRILFSFSFFDQIKSNEKSIDDKFSLQAVLPKWQLTAKDGLTWLRLNSVAQNPS
DVREAIERLWSIREKINKSPVRIVNDEKENFLVDNFSDDWKSQYRDALAKGIELINAGDLDKLVLATRQHLSLRKPLDPL
HLLARLRVQQTNSCRFLWQKNHDESFFGASPERLISLNQNQLLIDALAGTAKKGDDGRELLASSKDLREHHFVVNSIVEQ
LLKRGIKASYPSQPKLMTQDHLIHLHTLIQASVKEKSPLDLVEALHPTPAVAGLPLNKSLSWLRALEPFDRKTYASPIGW
IDKNQNSEFRVAIRYGQLKANELKLFAGAGLVKGSTVEGEMQEVALKFEVLRNQLNLDRVNCSNDL

Specific function: Involved in the synthesis of amonabactin, a phenolate siderophore containing 2,3-dihydroxybenzoic acid (2,3-DHB) [H]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=378, Percent_Identity=29.6296296296296, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI87082077, Length=265, Percent_Identity=31.6981132075472, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1788114, Length=285, Percent_Identity=24.2105263157895, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 52720; Mature: 52720

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEPVFSELLVSSLQKWSVRKVDECILSISVPVSQTDPLTTLPLIAEKHQFRFLWDLSP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHCCCCEEEEEECCC
GLCLSAGGHCQSLDLSGPKRFENAQRFSDEIFTRLIEISPSPAFSASRILFSFSFFDQIK
CCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SNEKSIDDKFSLQAVLPKWQLTAKDGLTWLRLNSVAQNPSDVREAIERLWSIREKINKSP
CCCCCCCHHHHHHHHCCCCEEECCCCCEEEEECHHHCCHHHHHHHHHHHHHHHHHHCCCC
VRIVNDEKENFLVDNFSDDWKSQYRDALAKGIELINAGDLDKLVLATRQHLSLRKPLDPL
EEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHH
HLLARLRVQQTNSCRFLWQKNHDESFFGASPERLISLNQNQLLIDALAGTAKKGDDGREL
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHEECCCCEEEHHHHHCCCCCCCCHHHH
LASSKDLREHHFVVNSIVEQLLKRGIKASYPSQPKLMTQDHLIHLHTLIQASVKEKSPLD
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHH
LVEALHPTPAVAGLPLNKSLSWLRALEPFDRKTYASPIGWIDKNQNSEFRVAIRYGQLKA
HHHHCCCCCCEECCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEEEECCCCC
NELKLFAGAGLVKGSTVEGEMQEVALKFEVLRNQLNLDRVNCSNDL
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNLEPVFSELLVSSLQKWSVRKVDECILSISVPVSQTDPLTTLPLIAEKHQFRFLWDLSP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHCCCCEEEEEECCC
GLCLSAGGHCQSLDLSGPKRFENAQRFSDEIFTRLIEISPSPAFSASRILFSFSFFDQIK
CCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
SNEKSIDDKFSLQAVLPKWQLTAKDGLTWLRLNSVAQNPSDVREAIERLWSIREKINKSP
CCCCCCCHHHHHHHHCCCCEEECCCCCEEEEECHHHCCHHHHHHHHHHHHHHHHHHCCCC
VRIVNDEKENFLVDNFSDDWKSQYRDALAKGIELINAGDLDKLVLATRQHLSLRKPLDPL
EEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHH
HLLARLRVQQTNSCRFLWQKNHDESFFGASPERLISLNQNQLLIDALAGTAKKGDDGREL
HHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHEECCCCEEEHHHHHCCCCCCCCHHHH
LASSKDLREHHFVVNSIVEQLLKRGIKASYPSQPKLMTQDHLIHLHTLIQASVKEKSPLD
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHH
LVEALHPTPAVAGLPLNKSLSWLRALEPFDRKTYASPIGWIDKNQNSEFRVAIRYGQLKA
HHHHCCCCCCEECCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEEEECCCCC
NELKLFAGAGLVKGSTVEGEMQEVALKFEVLRNQLNLDRVNCSNDL
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1830579 [H]