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The map label for this gene is cca [H]

Identifier: 124024926

GI number: 124024926

Start: 198070

End: 199257

Strand: Direct

Name: cca [H]

Synonym: NATL1_02131

Alternate gene names: 124024926

Gene position: 198070-199257 (Clockwise)

Preceding gene: 124024923

Following gene: 124024927

Centisome position: 10.62

GC content: 35.02

Gene sequence:

>1188_bases
TTGATAATTCAACACACATTACTATCAAATGATCTTTTTGGAGATTTAAATCCAAAAGATTGGCCTATATCCATAGCTGA
TTTGCCTCCTGGAAGTGCCTTAGTTGGAGGTTCTGTTAGAGATGGATTGTTGAAAAAATTGAGTCCAAAACCTGATTTAG
ATTTTGTTATTCCAGTAAATGCTATTGAGTTCAGCGAGAATTTATCTAAAAAGATTCATGCCACTTTTATAAAGCTTGAT
GAAAAGAGAGATATTGCTCGATTAGTTATCAATGGGTGGACTCTAGATTTTGCTCGACAAGTAGGAGAGAATCTGAAAGA
TGATTTATTGAGAAGAGATTTTCGAATAAATGCTATTGCTTTAAAGCTCAGAGAAAAACCAGAAATTTTTGACCCGATGG
GAGGAATGGATGATTTAAAAAATAACAAAATTGTTGCAATAAGCGAGAAGAACTTGCTTGATGATCCTTTGAGGCTCCTT
AGAGGTTTTCGATTAATGTGTGAATTAGATTTTGAGTTAGAACAAAAAACTAAAAGGTTTCTAAAAAATAATGTAGAAAA
ATTAAATAATGTTGCACCTGAGAGGATAAAAATGGAGGTCTTAAAAATTGCCCATTCAAAATGGAATTCCTCCGTTTGGC
AAACATATTCAGAACTTCAATTATTGAAAAGGTGGAATGACGATAATCTTAATGATAGAGAGCTAAAAAGAAAAGATATT
GCCTCAAATAATTCTTTATTTGGGAGCTTTTTGGCAAAGCTCATATTTCTTCTCAGTGATGAGGGCTTGTCAAGATTGAT
GTTTAGTAAAAACGAAATCAAAAGGTGCAAGAACTTGAGGTTTTGGGTTCATAAAATTAATCATTTAGATGATCTTTCCG
AAGATGAAAGATTTCAACTTCACATTGATTTGGAACAGGATTTGCCCTCTTTTATCCTTTTTTTGCAAGAAAGGGATTCA
AATTCTTGGCTAAAACGCTGGAAAGACCCCTCTGACCCTCTCTTTCACCCTTCTCCGCCCGTTAACGGCCATTTACTTCA
AAAGGTTTTTCAGCTCCCTCCTGGTCCGTTTTTAGGAGAACTTATTAGATATCTTTCTAAGGAAAAAGCATATGGAAGAT
TCTTCACGGATGCAGAGGCTTTGGAGGTTGCTCGTAAATGGACCCTAGAGAATTCACCCTTTTTGTGA

Upstream 100 bases:

>100_bases
CCATGATTTTCTTCAACTGTGTAGTAGGTCTTACAACTATCTACCCAAGCACAATCAACACATATGCACATAACTATTCA
AGGAAAGTTTAGGTGTCAAT

Downstream 100 bases:

>100_bases
TTAACTACACACAAATCAATTTAATCTCGGCCGTATGCCGTCGTCTTCTTATCCCCTTTTTTCTAAATGAGTGTTCGCCT
TTACATCGGTAATTTGCCGC

Product: poly A polymerase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MIIQHTLLSNDLFGDLNPKDWPISIADLPPGSALVGGSVRDGLLKKLSPKPDLDFVIPVNAIEFSENLSKKIHATFIKLD
EKRDIARLVINGWTLDFARQVGENLKDDLLRRDFRINAIALKLREKPEIFDPMGGMDDLKNNKIVAISEKNLLDDPLRLL
RGFRLMCELDFELEQKTKRFLKNNVEKLNNVAPERIKMEVLKIAHSKWNSSVWQTYSELQLLKRWNDDNLNDRELKRKDI
ASNNSLFGSFLAKLIFLLSDEGLSRLMFSKNEIKRCKNLRFWVHKINHLDDLSEDERFQLHIDLEQDLPSFILFLQERDS
NSWLKRWKDPSDPLFHPSPPVNGHLLQKVFQLPPGPFLGELIRYLSKEKAYGRFFTDAEALEVARKWTLENSPFL

Sequences:

>Translated_395_residues
MIIQHTLLSNDLFGDLNPKDWPISIADLPPGSALVGGSVRDGLLKKLSPKPDLDFVIPVNAIEFSENLSKKIHATFIKLD
EKRDIARLVINGWTLDFARQVGENLKDDLLRRDFRINAIALKLREKPEIFDPMGGMDDLKNNKIVAISEKNLLDDPLRLL
RGFRLMCELDFELEQKTKRFLKNNVEKLNNVAPERIKMEVLKIAHSKWNSSVWQTYSELQLLKRWNDDNLNDRELKRKDI
ASNNSLFGSFLAKLIFLLSDEGLSRLMFSKNEIKRCKNLRFWVHKINHLDDLSEDERFQLHIDLEQDLPSFILFLQERDS
NSWLKRWKDPSDPLFHPSPPVNGHLLQKVFQLPPGPFLGELIRYLSKEKAYGRFFTDAEALEVARKWTLENSPFL
>Mature_395_residues
MIIQHTLLSNDLFGDLNPKDWPISIADLPPGSALVGGSVRDGLLKKLSPKPDLDFVIPVNAIEFSENLSKKIHATFIKLD
EKRDIARLVINGWTLDFARQVGENLKDDLLRRDFRINAIALKLREKPEIFDPMGGMDDLKNNKIVAISEKNLLDDPLRLL
RGFRLMCELDFELEQKTKRFLKNNVEKLNNVAPERIKMEVLKIAHSKWNSSVWQTYSELQLLKRWNDDNLNDRELKRKDI
ASNNSLFGSFLAKLIFLLSDEGLSRLMFSKNEIKRCKNLRFWVHKINHLDDLSEDERFQLHIDLEQDLPSFILFLQERDS
NSWLKRWKDPSDPLFHPSPPVNGHLLQKVFQLPPGPFLGELIRYLSKEKAYGRFFTDAEALEVARKWTLENSPFL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45952; Mature: 45952

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIQHTLLSNDLFGDLNPKDWPISIADLPPGSALVGGSVRDGLLKKLSPKPDLDFVIPVN
CEEEEEHHCCCCCCCCCCCCCCEEEECCCCCCCEECCCHHHHHHHHCCCCCCCEEEEECC
AIEFSENLSKKIHATFIKLDEKRDIARLVINGWTLDFARQVGENLKDDLLRRDFRINAIA
HHHHHHHHHHHHHHHHEEECCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHCCCEEEEE
LKLREKPEIFDPMGGMDDLKNNKIVAISEKNLLDDPLRLLRGFRLMCELDFELEQKTKRF
EEECCCCCCCCCCCCHHHCCCCCEEEEECCCCCHHHHHHHHHCEEEEEECCHHHHHHHHH
LKNNVEKLNNVAPERIKMEVLKIAHSKWNSSVWQTYSELQLLKRWNDDNLNDRELKRKDI
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ASNNSLFGSFLAKLIFLLSDEGLSRLMFSKNEIKRCKNLRFWVHKINHLDDLSEDERFQL
CCCCCHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
HIDLEQDLPSFILFLQERDSNSWLKRWKDPSDPLFHPSPPVNGHLLQKVFQLPPGPFLGE
EEEHHHCCHHHEEHHHCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHH
LIRYLSKEKAYGRFFTDAEALEVARKWTLENSPFL
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIIQHTLLSNDLFGDLNPKDWPISIADLPPGSALVGGSVRDGLLKKLSPKPDLDFVIPVN
CEEEEEHHCCCCCCCCCCCCCCEEEECCCCCCCEECCCHHHHHHHHCCCCCCCEEEEECC
AIEFSENLSKKIHATFIKLDEKRDIARLVINGWTLDFARQVGENLKDDLLRRDFRINAIA
HHHHHHHHHHHHHHHHEEECCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHCCCEEEEE
LKLREKPEIFDPMGGMDDLKNNKIVAISEKNLLDDPLRLLRGFRLMCELDFELEQKTKRF
EEECCCCCCCCCCCCHHHCCCCCEEEEECCCCCHHHHHHHHHCEEEEEECCHHHHHHHHH
LKNNVEKLNNVAPERIKMEVLKIAHSKWNSSVWQTYSELQLLKRWNDDNLNDRELKRKDI
HHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
ASNNSLFGSFLAKLIFLLSDEGLSRLMFSKNEIKRCKNLRFWVHKINHLDDLSEDERFQL
CCCCCHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
HIDLEQDLPSFILFLQERDSNSWLKRWKDPSDPLFHPSPPVNGHLLQKVFQLPPGPFLGE
EEEHHHCCHHHEEHHHCCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHH
LIRYLSKEKAYGRFFTDAEALEVARKWTLENSPFL
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA