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The map label for this gene is cysK [H]

Identifier: 124024908

GI number: 124024908

Start: 181234

End: 182202

Strand: Reverse

Name: cysK [H]

Synonym: NATL1_01951

Alternate gene names: 124024908

Gene position: 182202-181234 (Counterclockwise)

Preceding gene: 124024909

Following gene: 124024907

Centisome position: 9.77

GC content: 40.87

Gene sequence:

>969_bases
ATGCCCATTGCTAATGACATAACTTATTTAGTTGGCCAAACACCTTTGGTCAAACTGAATCGATTACCTAATGAATTTAA
TTGTAGGGCTGAAATTATAGCCAAATTAGAAAGTTTCAACCCCACAGCATCCGTAAAAGACCGCATAGCTGGAGCAATGG
TGAAATCAGCAGAAAAAGAGGGCACTATCAAACCTGGACATACTGTTCTTGTTGAACCAACTAGCGGAAACACAGGAATT
GCATTAGCGATGGTTGCGGCAGCAAAAGGTTATCGGCTCATACTTACAATGCCTGACACAATGAGTACTGAACGACGCTC
AATGTTAAGAGCATTTGGAGCAGAGCTTCAACTAACTCCTGGCCAAGACGGTATCCAAGGCGCTATTCAGCTAGCAAAGG
AATTGGTTGCTTCTGTACCTAATGCATACTTGCTTCAGCAATTCGATAATTTATCCAATCCTGAAATTCATGAAAAAACA
ACCGCTGAAGAAATATGGGAAGATTGCGAAGGCAAACTTGATGCATTAATTGCAGGAGTAGGAACAGGAGGAACAATCAC
AGGTTGTGCCAGATTCTTAAAACAAAAAAATCCAAAAATCAAGGTTTTTGCAGTAGAACCTTCATCAAGCCCTGTTCTGT
CAGGAGGGAATCCTGGATCTCATGCGATACAAGGCATTGGGGCTGGTTTCATTCCTAATGTATTAGATATGAATCAGATT
GATGAAGTCATAAGAATCAATGATAATGAAGCAATGGACATAGGCAGAAGACTTGCCAAAGAAGAGGGATTGCTAAGTGG
TGTCAGCAGTGGTGCTGCAGTAGCCGCTGCTTTAAAAGTAGGAAATCAACCTGAGTTTGCGAATAAACGCTTGATTGTTA
TTCTGCCTAGTTTTGGTGAAAGATATCTATCAACAACAATGTTTACTTCTATCCCCGCAAAGCCAGTAAAAGGGAATGAG
TTTCTCTAA

Upstream 100 bases:

>100_bases
TAAAGCTTTTGAATAACAAATTTTCAAGCATTATTAAAAAAGAATTGAATTATTAAATAACTTTTGCCTCGAAACTAATT
CAAGTTTTTACAATAAGACC

Downstream 100 bases:

>100_bases
CAAGAAATAATCTTTCAACAATGAGCAAAGACCCATATCAAATTTTGAAAGTTTATCCCAGCGCAAAACTAGAAGAGATT
AAAAAAGCATACAGAAAACT

Product: O-acetylserine (thiol)-lyase A

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MPIANDITYLVGQTPLVKLNRLPNEFNCRAEIIAKLESFNPTASVKDRIAGAMVKSAEKEGTIKPGHTVLVEPTSGNTGI
ALAMVAAAKGYRLILTMPDTMSTERRSMLRAFGAELQLTPGQDGIQGAIQLAKELVASVPNAYLLQQFDNLSNPEIHEKT
TAEEIWEDCEGKLDALIAGVGTGGTITGCARFLKQKNPKIKVFAVEPSSSPVLSGGNPGSHAIQGIGAGFIPNVLDMNQI
DEVIRINDNEAMDIGRRLAKEEGLLSGVSSGAAVAAALKVGNQPEFANKRLIVILPSFGERYLSTTMFTSIPAKPVKGNE
FL

Sequences:

>Translated_322_residues
MPIANDITYLVGQTPLVKLNRLPNEFNCRAEIIAKLESFNPTASVKDRIAGAMVKSAEKEGTIKPGHTVLVEPTSGNTGI
ALAMVAAAKGYRLILTMPDTMSTERRSMLRAFGAELQLTPGQDGIQGAIQLAKELVASVPNAYLLQQFDNLSNPEIHEKT
TAEEIWEDCEGKLDALIAGVGTGGTITGCARFLKQKNPKIKVFAVEPSSSPVLSGGNPGSHAIQGIGAGFIPNVLDMNQI
DEVIRINDNEAMDIGRRLAKEEGLLSGVSSGAAVAAALKVGNQPEFANKRLIVILPSFGERYLSTTMFTSIPAKPVKGNE
FL
>Mature_321_residues
PIANDITYLVGQTPLVKLNRLPNEFNCRAEIIAKLESFNPTASVKDRIAGAMVKSAEKEGTIKPGHTVLVEPTSGNTGIA
LAMVAAAKGYRLILTMPDTMSTERRSMLRAFGAELQLTPGQDGIQGAIQLAKELVASVPNAYLLQQFDNLSNPEIHEKTT
AEEIWEDCEGKLDALIAGVGTGGTITGCARFLKQKNPKIKVFAVEPSSSPVLSGGNPGSHAIQGIGAGFIPNVLDMNQID
EVIRINDNEAMDIGRRLAKEEGLLSGVSSGAAVAAALKVGNQPEFANKRLIVILPSFGERYLSTTMFTSIPAKPVKGNEF
L

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=310, Percent_Identity=43.8709677419355, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI295821200, Length=310, Percent_Identity=43.8709677419355, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI4557415, Length=310, Percent_Identity=43.8709677419355, Blast_Score=259, Evalue=3e-69,
Organism=Escherichia coli, GI1788754, Length=318, Percent_Identity=56.9182389937107, Blast_Score=315, Evalue=2e-87,
Organism=Escherichia coli, GI2367138, Length=299, Percent_Identity=43.1438127090301, Blast_Score=226, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17535051, Length=302, Percent_Identity=49.6688741721854, Blast_Score=310, Evalue=6e-85,
Organism=Caenorhabditis elegans, GI115535073, Length=309, Percent_Identity=52.1035598705502, Blast_Score=305, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17562970, Length=301, Percent_Identity=48.8372093023256, Blast_Score=296, Evalue=8e-81,
Organism=Caenorhabditis elegans, GI32566674, Length=293, Percent_Identity=50.1706484641638, Blast_Score=291, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17561720, Length=301, Percent_Identity=48.1727574750831, Blast_Score=260, Evalue=8e-70,
Organism=Caenorhabditis elegans, GI32566672, Length=215, Percent_Identity=46.5116279069767, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17534315, Length=307, Percent_Identity=39.413680781759, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI25147552, Length=308, Percent_Identity=38.961038961039, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17561716, Length=121, Percent_Identity=42.9752066115703, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=40.6349206349206, Blast_Score=230, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321449, Length=327, Percent_Identity=35.7798165137615, Blast_Score=132, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24643623, Length=316, Percent_Identity=40.5063291139241, Blast_Score=204, Evalue=5e-53,
Organism=Drosophila melanogaster, GI20129101, Length=316, Percent_Identity=40.5063291139241, Blast_Score=204, Evalue=5e-53,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34298; Mature: 34167

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIANDITYLVGQTPLVKLNRLPNEFNCRAEIIAKLESFNPTASVKDRIAGAMVKSAEKE
CCCCCCHHHHHCCCCEEEHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
GTIKPGHTVLVEPTSGNTGIALAMVAAAKGYRLILTMPDTMSTERRSMLRAFGAELQLTP
CCCCCCCEEEEECCCCCCCHHHHEEHHCCCCEEEEECCCCCCHHHHHHHHHCCCCEEECC
GQDGIQGAIQLAKELVASVPNAYLLQQFDNLSNPEIHEKTTAEEIWEDCEGKLDALIAGV
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHEEEC
GTGGTITGCARFLKQKNPKIKVFAVEPSSSPVLSGGNPGSHAIQGIGAGFIPNVLDMNQI
CCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEECCCCCCHHHHHHCCCCCCCCCCCHHHH
DEVIRINDNEAMDIGRRLAKEEGLLSGVSSGAAVAAALKVGNQPEFANKRLIVILPSFGE
HHHEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCH
RYLSTTMFTSIPAKPVKGNEFL
HHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
PIANDITYLVGQTPLVKLNRLPNEFNCRAEIIAKLESFNPTASVKDRIAGAMVKSAEKE
CCCCCHHHHHCCCCEEEHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
GTIKPGHTVLVEPTSGNTGIALAMVAAAKGYRLILTMPDTMSTERRSMLRAFGAELQLTP
CCCCCCCEEEEECCCCCCCHHHHEEHHCCCCEEEEECCCCCCHHHHHHHHHCCCCEEECC
GQDGIQGAIQLAKELVASVPNAYLLQQFDNLSNPEIHEKTTAEEIWEDCEGKLDALIAGV
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHEEEC
GTGGTITGCARFLKQKNPKIKVFAVEPSSSPVLSGGNPGSHAIQGIGAGFIPNVLDMNQI
CCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEECCCCCCHHHHHHCCCCCCCCCCCHHHH
DEVIRINDNEAMDIGRRLAKEEGLLSGVSSGAAVAAALKVGNQPEFANKRLIVILPSFGE
HHHEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCH
RYLSTTMFTSIPAKPVKGNEFL
HHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]