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The map label for this gene is gatB

Identifier: 124024516

GI number: 124024516

Start: 2490827

End: 2492317

Strand: Reverse

Name: gatB

Synonym: P9303_28281

Alternate gene names: 124024516

Gene position: 2492317-2490827 (Counterclockwise)

Preceding gene: 124024518

Following gene: 124024514

Centisome position: 92.9

GC content: 55.2

Gene sequence:

>1491_bases
ATGACAGCGGCCACCAGCTCGGCTAACGGCGCATGGGAAGCCGTGATCGGTCTGGAGACACACGTGCAGCTAGGCACCAA
TAGCAAGATCTTCACATGCGCTTCAACTACCTTTGGCGACGACCCCAACACCCACATTGACCCTGTTGTGTGTGGCCTGC
CCGGCACGTTACCGGTGCTTAATCAGATGGTGTTGGAATACGCGGTCAAGGCAGCGATGGCACTCAACCTCAATATTGCC
GAGCACAGCAAGTTTGATCGCAAACAGTATTTCTATCCCGATCTACCGAAGAACTATCAGATCTCTCAATTTGATGAGCC
GATTGCAGAAGAAGGCTGGATTGAGGTGGAAGTTGCGGAGAAGGGGAAGGACACTTACCTCAAGCGTATTGGCATCGAGC
GTCTCCATATGGAGGAGGACGCCGGCAAGCTCGTTCATGCTGGCAGCGATCGACTCGCTGGCTCCACCCACTCCCTGGTG
GACTACAACCGGGCCGGCGTGGCGTTAGCTGAGATTGTCAGTAAGCCTGATCTGCGCACTGGGCGTGAGGCGGCTGAATA
TGCCTCGGAGATTCGCAGGATCATGCGTTATTTAGGGGTCTCTGATGGAAACATGCAGGAAGGCTCTTTGCGTTGTGACG
TCAATATCTCCGTGCGCCAAGGGGCCGACGCTCCCTTTGGCACCAAGGTTGAGATCAAAAACATGAATTCGTTTTCGGCC
ATCCAGAAAGCTTGTGAGTACGAGATTCAGCGCCAGATCAAGGCCTATGAGGCCGGTGAAGCAGTGGTGCAGGAAACCCG
CCTTTGGGATGAGGGCAAGCAACTCACCAAGAGCATGCGCTCGAAGGAAGGCAGCAGCGATTACCGCTATTTTCCGGATC
CTGATCTAGGTCCGATTGAGGTGATTGCATCTGTACGTGAGGGTTGGCGTACGGAGTTGCCAGAGTTGCCGTCGGCGAAG
CGTCATCGCTATGCCGAAGAGTTTGGTTTGTCGGTGTATGACGCCAGGGTGCTCACTGATGAATATGCGATGGCGGAATA
CTTCGAGGCGGCCGTGGCGGCAGGTGCCGAGGCCAAGGGAGTGGCGAATTGGATCCAGGGCGATATTGCTGCTTACGTGA
ATGCCAATCGCCTTTCTTATTCCACGTTGCCATTTCGACCGGAGCAGTTGGCAGAGATGGTGCAACTGATCGATGGCGGC
AAGATCAGCGGCAAGATCGCCAAGGAAATCTTGCCTGAGTTGCTGGAGAAGGGCGGCTCAGCTAAGGCGATTGTTGATCA
GCGCGGTCTGGGGATGATCAGTGATCCGGCGGCTATCACAACGATTGTGGAGGAACTGTTGGCGGCTCATCCTCAGGAAG
TAGAGGCATTCCGCGGCGGTAAGACCAAGCTGCAGGGTTTCTTTGTTGGGCAGCTCATGAAGAAGACTGGCGGCAAAGCC
GATCCCAAGCTCGCCAATCAGATCCTCAGCCAGAAGTTGAAGGGTGGATAG

Upstream 100 bases:

>100_bases
CAGATCAGTTCAGTGTTTCAGCCTGTTGACCCGACTTCGGCCATGTGAGGGAACCTTTCCATAGGATCAAGGACATATAC
CCAATAATTGGAGATCGGCA

Downstream 100 bases:

>100_bases
TTTTTTTTGCTGGGCTTGCCGCCCAGCACATGTTGAGTGCGCTGATGTAAAAACTTGAGATTTCGATGAGTAGTCTTATG
ATTGCTAGCAGTTCACAAAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MTAATSSANGAWEAVIGLETHVQLGTNSKIFTCASTTFGDDPNTHIDPVVCGLPGTLPVLNQMVLEYAVKAAMALNLNIA
EHSKFDRKQYFYPDLPKNYQISQFDEPIAEEGWIEVEVAEKGKDTYLKRIGIERLHMEEDAGKLVHAGSDRLAGSTHSLV
DYNRAGVALAEIVSKPDLRTGREAAEYASEIRRIMRYLGVSDGNMQEGSLRCDVNISVRQGADAPFGTKVEIKNMNSFSA
IQKACEYEIQRQIKAYEAGEAVVQETRLWDEGKQLTKSMRSKEGSSDYRYFPDPDLGPIEVIASVREGWRTELPELPSAK
RHRYAEEFGLSVYDARVLTDEYAMAEYFEAAVAAGAEAKGVANWIQGDIAAYVNANRLSYSTLPFRPEQLAEMVQLIDGG
KISGKIAKEILPELLEKGGSAKAIVDQRGLGMISDPAAITTIVEELLAAHPQEVEAFRGGKTKLQGFFVGQLMKKTGGKA
DPKLANQILSQKLKGG

Sequences:

>Translated_496_residues
MTAATSSANGAWEAVIGLETHVQLGTNSKIFTCASTTFGDDPNTHIDPVVCGLPGTLPVLNQMVLEYAVKAAMALNLNIA
EHSKFDRKQYFYPDLPKNYQISQFDEPIAEEGWIEVEVAEKGKDTYLKRIGIERLHMEEDAGKLVHAGSDRLAGSTHSLV
DYNRAGVALAEIVSKPDLRTGREAAEYASEIRRIMRYLGVSDGNMQEGSLRCDVNISVRQGADAPFGTKVEIKNMNSFSA
IQKACEYEIQRQIKAYEAGEAVVQETRLWDEGKQLTKSMRSKEGSSDYRYFPDPDLGPIEVIASVREGWRTELPELPSAK
RHRYAEEFGLSVYDARVLTDEYAMAEYFEAAVAAGAEAKGVANWIQGDIAAYVNANRLSYSTLPFRPEQLAEMVQLIDGG
KISGKIAKEILPELLEKGGSAKAIVDQRGLGMISDPAAITTIVEELLAAHPQEVEAFRGGKTKLQGFFVGQLMKKTGGKA
DPKLANQILSQKLKGG
>Mature_495_residues
TAATSSANGAWEAVIGLETHVQLGTNSKIFTCASTTFGDDPNTHIDPVVCGLPGTLPVLNQMVLEYAVKAAMALNLNIAE
HSKFDRKQYFYPDLPKNYQISQFDEPIAEEGWIEVEVAEKGKDTYLKRIGIERLHMEEDAGKLVHAGSDRLAGSTHSLVD
YNRAGVALAEIVSKPDLRTGREAAEYASEIRRIMRYLGVSDGNMQEGSLRCDVNISVRQGADAPFGTKVEIKNMNSFSAI
QKACEYEIQRQIKAYEAGEAVVQETRLWDEGKQLTKSMRSKEGSSDYRYFPDPDLGPIEVIASVREGWRTELPELPSAKR
HRYAEEFGLSVYDARVLTDEYAMAEYFEAAVAAGAEAKGVANWIQGDIAAYVNANRLSYSTLPFRPEQLAEMVQLIDGGK
ISGKIAKEILPELLEKGGSAKAIVDQRGLGMISDPAAITTIVEELLAAHPQEVEAFRGGKTKLQGFFVGQLMKKTGGKAD
PKLANQILSQKLKGG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=506, Percent_Identity=36.5612648221344, Blast_Score=315, Evalue=8e-86,
Organism=Caenorhabditis elegans, GI115534094, Length=305, Percent_Identity=40, Blast_Score=211, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=503, Percent_Identity=31.2127236580517, Blast_Score=223, Evalue=8e-59,
Organism=Drosophila melanogaster, GI45550808, Length=501, Percent_Identity=34.1317365269461, Blast_Score=290, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_PROM3 (A2CDJ8)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001018823.1
- STRING:   A2CDJ8
- GeneID:   4776962
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_28281
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54361; Mature: 54230

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAATSSANGAWEAVIGLETHVQLGTNSKIFTCASTTFGDDPNTHIDPVVCGLPGTLPVL
CCCCCCCCCCCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEECCCCHHHHH
NQMVLEYAVKAAMALNLNIAEHSKFDRKQYFYPDLPKNYQISQFDEPIAEEGWIEVEVAE
HHHHHHHHHHHHHHEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEECC
KGKDTYLKRIGIERLHMEEDAGKLVHAGSDRLAGSTHSLVDYNRAGVALAEIVSKPDLRT
CCHHHHHHHHCHHHHHHHHHCCCEEECCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCC
GREAAEYASEIRRIMRYLGVSDGNMQEGSLRCDVNISVRQGADAPFGTKVEIKNMNSFSA
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCHHHH
IQKACEYEIQRQIKAYEAGEAVVQETRLWDEGKQLTKSMRSKEGSSDYRYFPDPDLGPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
VIASVREGWRTELPELPSAKRHRYAEEFGLSVYDARVLTDEYAMAEYFEAAVAAGAEAKG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VANWIQGDIAAYVNANRLSYSTLPFRPEQLAEMVQLIDGGKISGKIAKEILPELLEKGGS
HHHHHHHHHHHEEECCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
AKAIVDQRGLGMISDPAAITTIVEELLAAHPQEVEAFRGGKTKLQGFFVGQLMKKTGGKA
CEEEHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
DPKLANQILSQKLKGG
CHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAATSSANGAWEAVIGLETHVQLGTNSKIFTCASTTFGDDPNTHIDPVVCGLPGTLPVL
CCCCCCCCCCEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCEEECCCCHHHHH
NQMVLEYAVKAAMALNLNIAEHSKFDRKQYFYPDLPKNYQISQFDEPIAEEGWIEVEVAE
HHHHHHHHHHHHHHEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEECC
KGKDTYLKRIGIERLHMEEDAGKLVHAGSDRLAGSTHSLVDYNRAGVALAEIVSKPDLRT
CCHHHHHHHHCHHHHHHHHHCCCEEECCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCC
GREAAEYASEIRRIMRYLGVSDGNMQEGSLRCDVNISVRQGADAPFGTKVEIKNMNSFSA
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCHHHH
IQKACEYEIQRQIKAYEAGEAVVQETRLWDEGKQLTKSMRSKEGSSDYRYFPDPDLGPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
VIASVREGWRTELPELPSAKRHRYAEEFGLSVYDARVLTDEYAMAEYFEAAVAAGAEAKG
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VANWIQGDIAAYVNANRLSYSTLPFRPEQLAEMVQLIDGGKISGKIAKEILPELLEKGGS
HHHHHHHHHHHEEECCCEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
AKAIVDQRGLGMISDPAAITTIVEELLAAHPQEVEAFRGGKTKLQGFFVGQLMKKTGGKA
CEEEHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
DPKLANQILSQKLKGG
CHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA