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The map label for this gene is miaE [H]

Identifier: 124023854

GI number: 124023854

Start: 1919683

End: 1920297

Strand: Reverse

Name: miaE [H]

Synonym: P9303_21611

Alternate gene names: 124023854

Gene position: 1920297-1919683 (Counterclockwise)

Preceding gene: 124023855

Following gene: 124023852

Centisome position: 71.58

GC content: 57.24

Gene sequence:

>615_bases
ATGAGTGCTCTTGCTGCTGCTAAGACGTCAAACGCCAAGATCAAATGGCTGGCGGCTCCCACCAGTGATGCTTGGTTGGA
GCAGGCGATTGCTCATCCGCTCGAGCTGCTGATTGATCACGCTCATTGCGAGCGCAAGGCGGCGGGTGCCGTCGTGCAAT
TGATGTTCCGCTACTTGTGTGAGCCTGGGCTCGCAGAAGTTCTCAGCCCTTTGGCAAGAGAGGAGCTGGAGCATTTTGAG
CGTGTGCTCACGTTGCTTAAGGCCAGAGGGCGTTACCTGGAGCCTCTGGCTGCGCCCCCTTATGGCGCAGTTTTGGCCAA
ACAGATCCGTAGGGATGAACCGCAGCGCATGCTCGATAGTTTTCTGGTGGCAGGGCTGATTGAAGCCCGTAGCCATGAAC
GCATGTCGTTGCTTGCCACCCACCTAGCTGATCTGGAGTTGCGTCAGCTCTATGCAGATCTCCTGGCGAGTGAGGCGCGT
CATTTCGGCCTCTATTGGAGGCTCTGTGAGCAGCGTTTTCAGCGTGAGCTGATCATTGCTCGTTTGCAGGAGTTGGCAGC
GGTGGAGGCAGGGATCCTCGCCAACTTGCATCCTCAGCCACGCATGCATAGCTGA

Upstream 100 bases:

>100_bases
CTGATCGCGCTGTGGGTGTGGTGAGCGGTTTTGGTGCATTGAGCTATCGCCTTGCTTTGGAAGGCTTGCTGGCTCATTTG
CGTCAGCCGCAGCAGGCTCT

Downstream 100 bases:

>100_bases
AGCCGATCAGCTTTATGGCAGTTTGGCGAATCTCTCGGCGATCGCATCCCCTGTGAAGCTTGCAATCCAGCCTTCTGGGT
TATCGAAGAAGCGCACAGCA

Product: tRNA-(MS[2]IO[6]A)-hydroxylase-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MSALAAAKTSNAKIKWLAAPTSDAWLEQAIAHPLELLIDHAHCERKAAGAVVQLMFRYLCEPGLAEVLSPLAREELEHFE
RVLTLLKARGRYLEPLAAPPYGAVLAKQIRRDEPQRMLDSFLVAGLIEARSHERMSLLATHLADLELRQLYADLLASEAR
HFGLYWRLCEQRFQRELIIARLQELAAVEAGILANLHPQPRMHS

Sequences:

>Translated_204_residues
MSALAAAKTSNAKIKWLAAPTSDAWLEQAIAHPLELLIDHAHCERKAAGAVVQLMFRYLCEPGLAEVLSPLAREELEHFE
RVLTLLKARGRYLEPLAAPPYGAVLAKQIRRDEPQRMLDSFLVAGLIEARSHERMSLLATHLADLELRQLYADLLASEAR
HFGLYWRLCEQRFQRELIIARLQELAAVEAGILANLHPQPRMHS
>Mature_203_residues
SALAAAKTSNAKIKWLAAPTSDAWLEQAIAHPLELLIDHAHCERKAAGAVVQLMFRYLCEPGLAEVLSPLAREELEHFER
VLTLLKARGRYLEPLAAPPYGAVLAKQIRRDEPQRMLDSFLVAGLIEARSHERMSLLATHLADLELRQLYADLLASEARH
FGLYWRLCEQRFQRELIIARLQELAAVEAGILANLHPQPRMHS

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 23011; Mature: 22880

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALAAAKTSNAKIKWLAAPTSDAWLEQAIAHPLELLIDHAHCERKAAGAVVQLMFRYLC
CCCHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPGLAEVLSPLAREELEHFERVLTLLKARGRYLEPLAAPPYGAVLAKQIRRDEPQRMLDS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHH
FLVAGLIEARSHERMSLLATHLADLELRQLYADLLASEARHFGLYWRLCEQRFQRELIIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLQELAAVEAGILANLHPQPRMHS
HHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SALAAAKTSNAKIKWLAAPTSDAWLEQAIAHPLELLIDHAHCERKAAGAVVQLMFRYLC
CCHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPGLAEVLSPLAREELEHFERVLTLLKARGRYLEPLAAPPYGAVLAKQIRRDEPQRMLDS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHH
FLVAGLIEARSHERMSLLATHLADLELRQLYADLLASEARHFGLYWRLCEQRFQRELIIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLQELAAVEAGILANLHPQPRMHS
HHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]