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The map label for this gene is hisF [H]

Identifier: 124023758

GI number: 124023758

Start: 1816689

End: 1817459

Strand: Reverse

Name: hisF [H]

Synonym: P9303_20641

Alternate gene names: 124023758

Gene position: 1817459-1816689 (Counterclockwise)

Preceding gene: 124023762

Following gene: 124023757

Centisome position: 67.75

GC content: 56.68

Gene sequence:

>771_bases
ATGGTCGCGCTTCGTTTGATTCCTTGCCTTGACGTGGCTGATGGTCGCGTTGTGAAGGGCGTGAACTTTGTGGGCCTTCG
TGATGCGGGTGATCCCGTGGAGCTTGCCTGCCGCTATAGCCAGGCTGGAGCTGATGAGTTGGTGTTTCTTGATATCGCTG
CCAGCCATGAAGGTCGGGCCACGCTGGTGGCATTAGTTCGACGTACTGCTGAGGCCGTGACCATTCCATTCACAGTTGGG
GGGGGAATCAGCTCTTTAGATGGCATCACAGAGTTGCTTCGGGCCGGAGCCGACAAGGTGAGTCTTAATTCCTCTGCTGT
GCGGGATCCAGATCTCGTAGCGCAGGGAGCCGACCGTTTTGGCTGTCAGTGCATTGTGGTGGCCATCGATGCTCGACGTC
GTCCTGAGGGCTCGGGGTGGGATGTTTTTGTCAAGGGTGGACGCCAAAACACAGGTTTGGATGTTGTCAGCTGGGCTCGG
CAGGCAGCGGAACTTGGTGCTGGTGAAATCCTGTTGACCTCAATGGATGGAGATGGAACTCAGGCTGGTTATGACTTGGA
GCTCACGCGAGCAGTCGTGGAAGCGGTGCCCGTGCCTGTAATCGCTTCGGGAGGTGCAGGTTGCCTGGATCACATCGCAG
AAGCCTTCACGATTGGCAAGGCCTCAGCTGCATTGTTGGCCTCTCTGCTTCACGATGGCGTGCTGAGTGTGGAGCAAATT
AAGACTGATCTGCTGATGCGAGGTTTGTCGATTCGGCCTCTTGAGGCCTGA

Upstream 100 bases:

>100_bases
CGGACCTTGGAGCCGATTCTGTAGGAAGTGGCAGCCTGGGACATGGCCTGCAAGGCTTAGAAGGTGGCGCAACCTTAACA
GTCGTCACTGCTTTATCCCA

Downstream 100 bases:

>100_bases
GGCTTTGTCTTTATGTTCAGCAACACACTCCTAAATTTGGCAGAAGTTCTGTTGGCGAGAGTGCTTTTGGATGTGTTGCT
GATCGATCACCCTTGGGCGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF1; ImGP synthase subunit hisF1; IGPS subunit hisF1 [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MVALRLIPCLDVADGRVVKGVNFVGLRDAGDPVELACRYSQAGADELVFLDIAASHEGRATLVALVRRTAEAVTIPFTVG
GGISSLDGITELLRAGADKVSLNSSAVRDPDLVAQGADRFGCQCIVVAIDARRRPEGSGWDVFVKGGRQNTGLDVVSWAR
QAAELGAGEILLTSMDGDGTQAGYDLELTRAVVEAVPVPVIASGGAGCLDHIAEAFTIGKASAALLASLLHDGVLSVEQI
KTDLLMRGLSIRPLEA

Sequences:

>Translated_256_residues
MVALRLIPCLDVADGRVVKGVNFVGLRDAGDPVELACRYSQAGADELVFLDIAASHEGRATLVALVRRTAEAVTIPFTVG
GGISSLDGITELLRAGADKVSLNSSAVRDPDLVAQGADRFGCQCIVVAIDARRRPEGSGWDVFVKGGRQNTGLDVVSWAR
QAAELGAGEILLTSMDGDGTQAGYDLELTRAVVEAVPVPVIASGGAGCLDHIAEAFTIGKASAALLASLLHDGVLSVEQI
KTDLLMRGLSIRPLEA
>Mature_256_residues
MVALRLIPCLDVADGRVVKGVNFVGLRDAGDPVELACRYSQAGADELVFLDIAASHEGRATLVALVRRTAEAVTIPFTVG
GGISSLDGITELLRAGADKVSLNSSAVRDPDLVAQGADRFGCQCIVVAIDARRRPEGSGWDVFVKGGRQNTGLDVVSWAR
QAAELGAGEILLTSMDGDGTQAGYDLELTRAVVEAVPVPVIASGGAGCLDHIAEAFTIGKASAALLASLLHDGVLSVEQI
KTDLLMRGLSIRPLEA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=46.09375, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI87082028, Length=212, Percent_Identity=31.6037735849057, Blast_Score=95, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=310, Percent_Identity=34.1935483870968, Blast_Score=154, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 26572; Mature: 26572

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVALRLIPCLDVADGRVVKGVNFVGLRDAGDPVELACRYSQAGADELVFLDIAASHEGRA
CCEEEEECCEECCCCCEEECEEEEEECCCCCHHHEEEECCCCCCCCEEEEEEECCCCCHH
TLVALVRRTAEAVTIPFTVGGGISSLDGITELLRAGADKVSLNSSAVRDPDLVAQGADRF
HHHHHHHHHHCEEEEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHCCCCCC
GCQCIVVAIDARRRPEGSGWDVFVKGGRQNTGLDVVSWARQAAELGAGEILLTSMDGDGT
CCEEEEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCC
QAGYDLELTRAVVEAVPVPVIASGGAGCLDHIAEAFTIGKASAALLASLLHDGVLSVEQI
CCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHH
KTDLLMRGLSIRPLEA
HHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVALRLIPCLDVADGRVVKGVNFVGLRDAGDPVELACRYSQAGADELVFLDIAASHEGRA
CCEEEEECCEECCCCCEEECEEEEEECCCCCHHHEEEECCCCCCCCEEEEEEECCCCCHH
TLVALVRRTAEAVTIPFTVGGGISSLDGITELLRAGADKVSLNSSAVRDPDLVAQGADRF
HHHHHHHHHHCEEEEEEEECCCCHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHCCCCCC
GCQCIVVAIDARRRPEGSGWDVFVKGGRQNTGLDVVSWARQAAELGAGEILLTSMDGDGT
CCEEEEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCC
QAGYDLELTRAVVEAVPVPVIASGGAGCLDHIAEAFTIGKASAALLASLLHDGVLSVEQI
CCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHH
KTDLLMRGLSIRPLEA
HHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]