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The map label for this gene is lpd [H]

Identifier: 124023678

GI number: 124023678

Start: 1739137

End: 1740606

Strand: Reverse

Name: lpd [H]

Synonym: P9303_19781

Alternate gene names: 124023678

Gene position: 1740606-1739137 (Counterclockwise)

Preceding gene: 124023679

Following gene: 124023677

Centisome position: 64.88

GC content: 55.37

Gene sequence:

>1470_bases
GTGAGCGAAGCCAGTTTCGACTTCGATGTGATCGTGATCGGTGCGGGTTACGGCGGTTTTGATGCCGCCAAGCATGCCGC
TGATGATGGCCTCAAGGTGGCGATTGTGGAATCAGGTGATATGGGCGGCACTTGCGTGAATCGAGGATGTGTGCCCTCTA
AGGCATTGCTTGCTGCCAGTGGAAAGGTGCGTGAGCTGGCTGATGCTGAACATCTATCTGGTTTCGGCATCCATGCTGCA
CCGGTGCGTTTTGAACGCCAGAAGATTGCTGACCATGCCAACAAGTTGGTGGCCACGATTCGTAGCAACCTCACCAAAAC
GCTGCAGCGGGCTGGTGTCACGATCCTGCGTGGCCATGGACGATTGGAGGGAAGCCAGCGAATTGGCGTAAGAGAGAAAA
GTGGAGTGGATCGGCTGCTCACTGCGAGGGATGTGATCCTTGCCACAGGCTCTGATCCATTCGTGCCCCCTGGGATTGAA
ACCGATGGACGCACGGTTTTTACCAGTGATGAAGCCGTGAACTTGGAATGGCTGCCGCGTTGGATTGCAATTATCGGCAG
TGGTTATATCGGCCTTGAATTTGCCGATGTTTATACGGCTCTCGGCTGTGAAGTCACGATGATTGAGGCGTTGGATCGGG
TCATGCCAACCTTTGATCCCGACATCACCAAGATGGCAGCTCGGCATTTGATTGAAGGACGTGACATCGATGCACGTGCT
GGTGTGCTGGCTAGCAAGGTGATCCCTGGCTGCCCGGTAAGGATCGAACTGGCGGAGATGAAAAGCCGTGAGCTGGTGGA
CAGCTTGGAGGTGGATGCGGTGCTCGTCGCTACTGGTCGGGTGCCAAGTAGCAAGGGGCTCAATCTTGAGTCGGTTGGTG
TGGAGACCAATCGCGGCTTTGTGCCGATTGACGACAGCATGAGGGTATTGGTCAACGGAAACCCGCTGCCTCATCTTTGG
GCCGTCGGTGATGTCACTGGCAAGCTGATGTTGGCCCATACAGCCGCAGCACAGGGCACGCTGGCTGTTGACAACATCCA
GGGACACTCCCGCACGATTGATTACCGCAGCATTCCAGCCGCGACCTTCACTCATCCGGAGATCAGCTCAGTGGGGTTGA
GTGAAGCTGATGCCAAAGAGCTTGCCGCCAAGGATGGGTTTGAGTTGGGGAGTGTGCGCAGCTATTTCAAGGCCAATTCC
AAAGCACTAGCAGAGCTGGAGAGTGATGGATTGATGAAGTTGTTGTTCCGCAAGGACAGCGGTGAAGTTCTTGGTGCTCA
TATCTATGGATTGCACGCCGCCGATTTGATCCAGGAGGTAGCCAATGCGGTGGCACGACGTCAGAGCGTTGCTCAATTGG
CCAATGAAGTGCATACCCATCCCACCCTGAGTGAAGTGGTGGAAGTGGCCTATAAACAGGCGGCGAAACAGCTGGCTAAG
GCGGTTGTTGCATCGGCCAGTGCTTCCTAG

Upstream 100 bases:

>100_bases
ATAGCGCAGCAGTTGAGTCGCTCAACGTGGCGGCGGTGGCGGTGCCACTATTGCTTGAGCGGCGACGAGCCACAATGACC
TTCTCCACGCAGTAGTTCGG

Downstream 100 bases:

>100_bases
GCCGCTGTTCTTCAAGCAGGTCTTAGCCCTGCGGTTCACTGTTTTTAATTGCACTATCACCCGATGGAGATCCGCAGACG
TCCGCCCAATCCAAGCGTCA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; LPD; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSEASFDFDVIVIGAGYGGFDAAKHAADDGLKVAIVESGDMGGTCVNRGCVPSKALLAASGKVRELADAEHLSGFGIHAA
PVRFERQKIADHANKLVATIRSNLTKTLQRAGVTILRGHGRLEGSQRIGVREKSGVDRLLTARDVILATGSDPFVPPGIE
TDGRTVFTSDEAVNLEWLPRWIAIIGSGYIGLEFADVYTALGCEVTMIEALDRVMPTFDPDITKMAARHLIEGRDIDARA
GVLASKVIPGCPVRIELAEMKSRELVDSLEVDAVLVATGRVPSSKGLNLESVGVETNRGFVPIDDSMRVLVNGNPLPHLW
AVGDVTGKLMLAHTAAAQGTLAVDNIQGHSRTIDYRSIPAATFTHPEISSVGLSEADAKELAAKDGFELGSVRSYFKANS
KALAELESDGLMKLLFRKDSGEVLGAHIYGLHAADLIQEVANAVARRQSVAQLANEVHTHPTLSEVVEVAYKQAAKQLAK
AVVASASAS

Sequences:

>Translated_489_residues
MSEASFDFDVIVIGAGYGGFDAAKHAADDGLKVAIVESGDMGGTCVNRGCVPSKALLAASGKVRELADAEHLSGFGIHAA
PVRFERQKIADHANKLVATIRSNLTKTLQRAGVTILRGHGRLEGSQRIGVREKSGVDRLLTARDVILATGSDPFVPPGIE
TDGRTVFTSDEAVNLEWLPRWIAIIGSGYIGLEFADVYTALGCEVTMIEALDRVMPTFDPDITKMAARHLIEGRDIDARA
GVLASKVIPGCPVRIELAEMKSRELVDSLEVDAVLVATGRVPSSKGLNLESVGVETNRGFVPIDDSMRVLVNGNPLPHLW
AVGDVTGKLMLAHTAAAQGTLAVDNIQGHSRTIDYRSIPAATFTHPEISSVGLSEADAKELAAKDGFELGSVRSYFKANS
KALAELESDGLMKLLFRKDSGEVLGAHIYGLHAADLIQEVANAVARRQSVAQLANEVHTHPTLSEVVEVAYKQAAKQLAK
AVVASASAS
>Mature_488_residues
SEASFDFDVIVIGAGYGGFDAAKHAADDGLKVAIVESGDMGGTCVNRGCVPSKALLAASGKVRELADAEHLSGFGIHAAP
VRFERQKIADHANKLVATIRSNLTKTLQRAGVTILRGHGRLEGSQRIGVREKSGVDRLLTARDVILATGSDPFVPPGIET
DGRTVFTSDEAVNLEWLPRWIAIIGSGYIGLEFADVYTALGCEVTMIEALDRVMPTFDPDITKMAARHLIEGRDIDARAG
VLASKVIPGCPVRIELAEMKSRELVDSLEVDAVLVATGRVPSSKGLNLESVGVETNRGFVPIDDSMRVLVNGNPLPHLWA
VGDVTGKLMLAHTAAAQGTLAVDNIQGHSRTIDYRSIPAATFTHPEISSVGLSEADAKELAAKDGFELGSVRSYFKANSK
ALAELESDGLMKLLFRKDSGEVLGAHIYGLHAADLIQEVANAVARRQSVAQLANEVHTHPTLSEVVEVAYKQAAKQLAKA
VVASASAS

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=479, Percent_Identity=32.1503131524008, Blast_Score=243, Evalue=4e-64,
Organism=Homo sapiens, GI50301238, Length=475, Percent_Identity=29.6842105263158, Blast_Score=182, Evalue=9e-46,
Organism=Homo sapiens, GI22035672, Length=466, Percent_Identity=29.6137339055794, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI148277065, Length=488, Percent_Identity=26.844262295082, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI33519430, Length=488, Percent_Identity=26.844262295082, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI33519428, Length=488, Percent_Identity=26.844262295082, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI33519426, Length=488, Percent_Identity=26.844262295082, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI148277071, Length=488, Percent_Identity=26.844262295082, Blast_Score=143, Evalue=5e-34,
Organism=Homo sapiens, GI291045266, Length=483, Percent_Identity=27.3291925465839, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI291045268, Length=480, Percent_Identity=24.7916666666667, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786307, Length=464, Percent_Identity=32.7586206896552, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082354, Length=478, Percent_Identity=28.6610878661088, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789915, Length=449, Percent_Identity=29.1759465478842, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI87081717, Length=467, Percent_Identity=27.1948608137045, Blast_Score=131, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI32565766, Length=482, Percent_Identity=32.1576763485477, Blast_Score=220, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17557007, Length=485, Percent_Identity=29.0721649484536, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71983419, Length=472, Percent_Identity=29.4491525423729, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=472, Percent_Identity=29.4491525423729, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71982272, Length=497, Percent_Identity=26.7605633802817, Blast_Score=118, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=32.9875518672199, Blast_Score=216, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6325166, Length=482, Percent_Identity=28.2157676348548, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6325240, Length=488, Percent_Identity=27.6639344262295, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21358499, Length=485, Percent_Identity=32.9896907216495, Blast_Score=240, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24640549, Length=497, Percent_Identity=28.9738430583501, Blast_Score=142, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24640553, Length=495, Percent_Identity=29.0909090909091, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24640551, Length=495, Percent_Identity=29.0909090909091, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17737741, Length=496, Percent_Identity=27.8225806451613, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51881; Mature: 51749

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEASFDFDVIVIGAGYGGFDAAKHAADDGLKVAIVESGDMGGTCVNRGCVPSKALLAAS
CCCCCCCEEEEEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCC
GKVRELADAEHLSGFGIHAAPVRFERQKIADHANKLVATIRSNLTKTLQRAGVTILRGHG
CCHHHHHCHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
RLEGSQRIGVREKSGVDRLLTARDVILATGSDPFVPPGIETDGRTVFTSDEAVNLEWLPR
CCCCCCCCCCCCCCCHHHHHHHCEEEEECCCCCCCCCCCCCCCCEEEECCCEECHHHHHH
WIAIIGSGYIGLEFADVYTALGCEVTMIEALDRVMPTFDPDITKMAARHLIEGRDIDARA
HHHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
GVLASKVIPGCPVRIELAEMKSRELVDSLEVDAVLVATGRVPSSKGLNLESVGVETNRGF
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECEECCCCE
VPIDDSMRVLVNGNPLPHLWAVGDVTGKLMLAHTAAAQGTLAVDNIQGHSRTIDYRSIPA
EECCCCCEEEECCCCCCCEEEECCCCCEEEEEEHHCCCCCEEEECCCCCCEEECCCCCCC
ATFTHPEISSVGLSEADAKELAAKDGFELGSVRSYFKANSKALAELESDGLMKLLFRKDS
CCCCCCCHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCC
GEVLGAHIYGLHAADLIQEVANAVARRQSVAQLANEVHTHPTLSEVVEVAYKQAAKQLAK
CCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AVVASASAS
HHHHHCCCC
>Mature Secondary Structure 
SEASFDFDVIVIGAGYGGFDAAKHAADDGLKVAIVESGDMGGTCVNRGCVPSKALLAAS
CCCCCCEEEEEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCC
GKVRELADAEHLSGFGIHAAPVRFERQKIADHANKLVATIRSNLTKTLQRAGVTILRGHG
CCHHHHHCHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
RLEGSQRIGVREKSGVDRLLTARDVILATGSDPFVPPGIETDGRTVFTSDEAVNLEWLPR
CCCCCCCCCCCCCCCHHHHHHHCEEEEECCCCCCCCCCCCCCCCEEEECCCEECHHHHHH
WIAIIGSGYIGLEFADVYTALGCEVTMIEALDRVMPTFDPDITKMAARHLIEGRDIDARA
HHHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
GVLASKVIPGCPVRIELAEMKSRELVDSLEVDAVLVATGRVPSSKGLNLESVGVETNRGF
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECEECCCCE
VPIDDSMRVLVNGNPLPHLWAVGDVTGKLMLAHTAAAQGTLAVDNIQGHSRTIDYRSIPA
EECCCCCEEEECCCCCCCEEEECCCCCEEEEEEHHCCCCCEEEECCCCCCEEECCCCCCC
ATFTHPEISSVGLSEADAKELAAKDGFELGSVRSYFKANSKALAELESDGLMKLLFRKDS
CCCCCCCHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCC
GEVLGAHIYGLHAADLIQEVANAVARRQSVAQLANEVHTHPTLSEVVEVAYKQAAKQLAK
CCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AVVASASAS
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231; 9387233 [H]