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The map label for this gene is nth [H]

Identifier: 124023464

GI number: 124023464

Start: 1540372

End: 1541025

Strand: Reverse

Name: nth [H]

Synonym: P9303_17641

Alternate gene names: 124023464

Gene position: 1541025-1540372 (Counterclockwise)

Preceding gene: 124023465

Following gene: 124023461

Centisome position: 57.44

GC content: 43.73

Gene sequence:

>654_bases
GTGAGAAAACATCAACGAGCAGAAACGATCATGCGACGACTAAACGAGCACTATCCTGACCCTGCCATTCCTCTCTACCA
TCATGACGATTTCACCCTATTGATTGCAGTTGTTTTAAGTGCGCAGTGCACTGACAAGAAAGTCAATGAGGTAACAGTAT
CTCTGTTTGAACATGCACAGACCCCAGAGGAGATGTATCAGTTAGGTGAAGTGAAAATACTAACTATGATCAGACAGCTA
GGCCTGTCAAAGCAAAAAGCCAAAAACATACATAGACTTTCGGGAATCATTGTTCAACGCTTCCATTCTTCCGTACCACA
GAATTTCGATGATCTAGAATCACTTCCTGGAGTGGGACACAAAACAGCAAGTGTTGTTATGGCCCAAGCTTTTGGAGTGC
CTACATTTCCAGTTGATACGCATATCCACCGTTTAGCCCAAAGATGGGGATTATCTAATGGCAGCAGCGTGGTTCAGACA
GAAAAAGATCTCAAAAAGCTGTTCCCGAAATCAGCATGGAATAAGCTTCACCTCCAGATCATCTACTATGGGCGTGAAAA
CTGTTCGGCAAGAGGGTGTGATGGAACAACCTGTGATTTATGTAGAGAGTTATATCCAAAACGACGCAATCCAGTGAAGT
GGATCAAAGCCTAG

Upstream 100 bases:

>100_bases
CACAGGTAGATAAGCAAGAGTAGATGTTCAAAAAGAAATTTTGGACAATGAATGATAAAAAGCAAACAAACACCGCAATT
CGCCATTTAATGAAAGACAC

Downstream 100 bases:

>100_bases
CTCGATAAATAATCAGATATCATCTATAATTATGCATGCGCAAATGACGTCAAGAATTATATGAACATATATAGCTAGAA
AAATATTTTATTGTCAACCC

Product: endonuclease

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MRKHQRAETIMRRLNEHYPDPAIPLYHHDDFTLLIAVVLSAQCTDKKVNEVTVSLFEHAQTPEEMYQLGEVKILTMIRQL
GLSKQKAKNIHRLSGIIVQRFHSSVPQNFDDLESLPGVGHKTASVVMAQAFGVPTFPVDTHIHRLAQRWGLSNGSSVVQT
EKDLKKLFPKSAWNKLHLQIIYYGRENCSARGCDGTTCDLCRELYPKRRNPVKWIKA

Sequences:

>Translated_217_residues
MRKHQRAETIMRRLNEHYPDPAIPLYHHDDFTLLIAVVLSAQCTDKKVNEVTVSLFEHAQTPEEMYQLGEVKILTMIRQL
GLSKQKAKNIHRLSGIIVQRFHSSVPQNFDDLESLPGVGHKTASVVMAQAFGVPTFPVDTHIHRLAQRWGLSNGSSVVQT
EKDLKKLFPKSAWNKLHLQIIYYGRENCSARGCDGTTCDLCRELYPKRRNPVKWIKA
>Mature_217_residues
MRKHQRAETIMRRLNEHYPDPAIPLYHHDDFTLLIAVVLSAQCTDKKVNEVTVSLFEHAQTPEEMYQLGEVKILTMIRQL
GLSKQKAKNIHRLSGIIVQRFHSSVPQNFDDLESLPGVGHKTASVVMAQAFGVPTFPVDTHIHRLAQRWGLSNGSSVVQT
EKDLKKLFPKSAWNKLHLQIIYYGRENCSARGCDGTTCDLCRELYPKRRNPVKWIKA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=161, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1787920, Length=204, Percent_Identity=42.156862745098, Blast_Score=146, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17554540, Length=189, Percent_Identity=31.7460317460317, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324530, Length=182, Percent_Identity=27.4725274725275, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6319304, Length=175, Percent_Identity=26.8571428571429, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=28.8135593220339, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24811; Mature: 24811

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKHQRAETIMRRLNEHYPDPAIPLYHHDDFTLLIAVVLSAQCTDKKVNEVTVSLFEHAQ
CCHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
TPEEMYQLGEVKILTMIRQLGLSKQKAKNIHRLSGIIVQRFHSSVPQNFDDLESLPGVGH
CHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCC
KTASVVMAQAFGVPTFPVDTHIHRLAQRWGLSNGSSVVQTEKDLKKLFPKSAWNKLHLQI
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHCCCEEEEEE
IYYGRENCSARGCDGTTCDLCRELYPKRRNPVKWIKA
EEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCHHCCC
>Mature Secondary Structure
MRKHQRAETIMRRLNEHYPDPAIPLYHHDDFTLLIAVVLSAQCTDKKVNEVTVSLFEHAQ
CCHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
TPEEMYQLGEVKILTMIRQLGLSKQKAKNIHRLSGIIVQRFHSSVPQNFDDLESLPGVGH
CHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCC
KTASVVMAQAFGVPTFPVDTHIHRLAQRWGLSNGSSVVQTEKDLKKLFPKSAWNKLHLQI
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHCCCEEEEEE
IYYGRENCSARGCDGTTCDLCRELYPKRRNPVKWIKA
EEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]