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The map label for this gene is proB [H]

Identifier: 124023436

GI number: 124023436

Start: 1519207

End: 1520292

Strand: Reverse

Name: proB [H]

Synonym: P9303_17361

Alternate gene names: 124023436

Gene position: 1520292-1519207 (Counterclockwise)

Preceding gene: 124023437

Following gene: 124023435

Centisome position: 56.67

GC content: 56.91

Gene sequence:

>1086_bases
ATGAGTCTGTGGGTGGTGAAGGTGGGAACCAGCCTGCTGAGAGGCAATGAGAAGCAATCCACCGCCGAAGTGATTGAGAG
CTACAGCGCCTGTCTTTCAGCAAGCCTCAAGCGGGGGGATCATGTTGTCCTCGTAACAAGTGGCGCCGTAGGTCTGGGTT
GCAATCGTCTAGGGCTAAGCCAACGGCCGGTTGATGTGGTGGCTCTCCAAGCGACGGCGGCTATCGGCCAAGGACAACTC
ATGGCTCTCTATGAGGCTGCCATGAGCCGAAGGGGCTACACCGTGGCTCAGGTATTGCTCACCCGCTCAGATCTTGGCTC
GAGGCAGCGATACCGCAATGCTTCAAGCACCTTGAAGCAATTACTCGACTGGGGCGTGCTGCCGGTGGTGAATGAGAACG
ACGCCCTCTCTCCGGAAGAATTGCGTTACGGCGACAACGACACCCTTTCAGCTCTCGTTGCAACCGCAGTGGAGGCTGAT
CAGCTCATCATGCTCACCGACGTGGATCGCCTTTATTCCTCAGATCCCCGCATTAATGCCTCTGCCGAACCGATCTCAGA
TGTTTACCATCCCCATGAACTGACTGCCCTGGAAGTCGCGGCAGGGGAGGGAGGTCCCTGGGGTACTGGGGGTATGAGTA
CAAAACTTGCGGCAGCTCGCATCGCTACCGCAAGTGGCATCACCGTTCTTCTTGCTGATGGCCGCGATCCTCAAGTGCTT
GATGGGTTGCTGCAAGGAAGACGGAGCGGCACGGTGTTTCATCCTCACCCTCAACCTCTCGGTAATCGCAAGAGCTGGCT
GGCTCATGCCCTCAAGCCCTTAGGGACCCTTCAGCTGGATGAAGGCGCCTGCGATGCCCTTCAACATCGTGGCGCCTCCC
TACTCTTGGTGGGGGTTAAAGAGGTGGAAGGAAACTTTGAAGCCAACCAGCCGGTAAGACTAATTAATCCCGAAGGTAAC
GAGCTCGCTCGAGGCTTGTGTTCCTTGAGTAGCAATGAACTGCGCGAGGCCATTGAGACCCAAATGAGCACGAATCGTTC
ACCGGTCGTGGTGCATAGGGACGTGTTGGTTCTCCGCAACGCGTGA

Upstream 100 bases:

>100_bases
TTCTAGTTCGACCACTACGCGCCGATGGTTACCCCTGCAACAACACTCGAGTTCAGCGTCTTGAGCAAAAACTTGCCGCA
TGGCTAGGGGCCCCACAGAC

Downstream 100 bases:

>100_bases
CTTGAGTGAACATCAACAACACATCACGCCTACGATCCGCCGAAAAGTGATTTGACATGCGCTTCAGCCAGTTGATCGCC
TCCCTCCAACAAGGAAGCGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSLWVVKVGTSLLRGNEKQSTAEVIESYSACLSASLKRGDHVVLVTSGAVGLGCNRLGLSQRPVDVVALQATAAIGQGQL
MALYEAAMSRRGYTVAQVLLTRSDLGSRQRYRNASSTLKQLLDWGVLPVVNENDALSPEELRYGDNDTLSALVATAVEAD
QLIMLTDVDRLYSSDPRINASAEPISDVYHPHELTALEVAAGEGGPWGTGGMSTKLAAARIATASGITVLLADGRDPQVL
DGLLQGRRSGTVFHPHPQPLGNRKSWLAHALKPLGTLQLDEGACDALQHRGASLLLVGVKEVEGNFEANQPVRLINPEGN
ELARGLCSLSSNELREAIETQMSTNRSPVVVHRDVLVLRNA

Sequences:

>Translated_361_residues
MSLWVVKVGTSLLRGNEKQSTAEVIESYSACLSASLKRGDHVVLVTSGAVGLGCNRLGLSQRPVDVVALQATAAIGQGQL
MALYEAAMSRRGYTVAQVLLTRSDLGSRQRYRNASSTLKQLLDWGVLPVVNENDALSPEELRYGDNDTLSALVATAVEAD
QLIMLTDVDRLYSSDPRINASAEPISDVYHPHELTALEVAAGEGGPWGTGGMSTKLAAARIATASGITVLLADGRDPQVL
DGLLQGRRSGTVFHPHPQPLGNRKSWLAHALKPLGTLQLDEGACDALQHRGASLLLVGVKEVEGNFEANQPVRLINPEGN
ELARGLCSLSSNELREAIETQMSTNRSPVVVHRDVLVLRNA
>Mature_360_residues
SLWVVKVGTSLLRGNEKQSTAEVIESYSACLSASLKRGDHVVLVTSGAVGLGCNRLGLSQRPVDVVALQATAAIGQGQLM
ALYEAAMSRRGYTVAQVLLTRSDLGSRQRYRNASSTLKQLLDWGVLPVVNENDALSPEELRYGDNDTLSALVATAVEADQ
LIMLTDVDRLYSSDPRINASAEPISDVYHPHELTALEVAAGEGGPWGTGGMSTKLAAARIATASGITVLLADGRDPQVLD
GLLQGRRSGTVFHPHPQPLGNRKSWLAHALKPLGTLQLDEGACDALQHRGASLLLVGVKEVEGNFEANQPVRLINPEGNE
LARGLCSLSSNELREAIETQMSTNRSPVVVHRDVLVLRNA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=282, Percent_Identity=31.9148936170213, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI21361368, Length=284, Percent_Identity=31.6901408450704, Blast_Score=125, Evalue=6e-29,
Organism=Escherichia coli, GI1786437, Length=365, Percent_Identity=42.1917808219178, Blast_Score=262, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17569967, Length=265, Percent_Identity=32.4528301886792, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17569969, Length=265, Percent_Identity=32.4528301886792, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6320506, Length=413, Percent_Identity=32.4455205811138, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321822, Length=349, Percent_Identity=36.1031518624642, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI21357643, Length=285, Percent_Identity=31.2280701754386, Blast_Score=128, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 38549; Mature: 38418

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLWVVKVGTSLLRGNEKQSTAEVIESYSACLSASLKRGDHVVLVTSGAVGLGCNRLGLS
CEEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
QRPVDVVALQATAAIGQGQLMALYEAAMSRRGYTVAQVLLTRSDLGSRQRYRNASSTLKQ
CCCCEEEEEEHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCCHHHHHHH
LLDWGVLPVVNENDALSPEELRYGDNDTLSALVATAVEADQLIMLTDVDRLYSSDPRINA
HHHCCCCEEECCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCC
SAEPISDVYHPHELTALEVAAGEGGPWGTGGMSTKLAAARIATASGITVLLADGRDPQVL
CCCCHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHEECCCCEEEEECCCCHHHH
DGLLQGRRSGTVFHPHPQPLGNRKSWLAHALKPLGTLQLDEGACDALQHRGASLLLVGVK
HHHHHCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHCCCEEEEEEEE
EVEGNFEANQPVRLINPEGNELARGLCSLSSNELREAIETQMSTNRSPVVVHRDVLVLRN
ECCCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCEEEEEC
A
C
>Mature Secondary Structure 
SLWVVKVGTSLLRGNEKQSTAEVIESYSACLSASLKRGDHVVLVTSGAVGLGCNRLGLS
EEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
QRPVDVVALQATAAIGQGQLMALYEAAMSRRGYTVAQVLLTRSDLGSRQRYRNASSTLKQ
CCCCEEEEEEHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCCHHHHHHH
LLDWGVLPVVNENDALSPEELRYGDNDTLSALVATAVEADQLIMLTDVDRLYSSDPRINA
HHHCCCCEEECCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHCCCCCCCC
SAEPISDVYHPHELTALEVAAGEGGPWGTGGMSTKLAAARIATASGITVLLADGRDPQVL
CCCCHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHEECCCCEEEEECCCCHHHH
DGLLQGRRSGTVFHPHPQPLGNRKSWLAHALKPLGTLQLDEGACDALQHRGASLLLVGVK
HHHHHCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHCCCEEEEEEEE
EVEGNFEANQPVRLINPEGNELARGLCSLSSNELREAIETQMSTNRSPVVVHRDVLVLRN
ECCCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCEEEEEC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]