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The map label for this gene is pdhB [H]

Identifier: 124023323

GI number: 124023323

Start: 1420071

End: 1421054

Strand: Direct

Name: pdhB [H]

Synonym: P9303_16211

Alternate gene names: 124023323

Gene position: 1420071-1421054 (Clockwise)

Preceding gene: 124023322

Following gene: 124023324

Centisome position: 52.93

GC content: 51.22

Gene sequence:

>984_bases
GTGTCAGGGACGCTTCTCTTTAATGCTCTTCGAGATGCCATCGATGAAGAGATGGCCAGAGATTCGCATGTTTGTGTGAT
GGGAGAGGACGTCGGCCAATACGGCGGCTCCTACAAGGTCACCAAGGATCTCTACGAGAAATATGGCGAGTTGCGGGTGT
TGGATACACCGATTGCCGAGAACAGTTTTACGGGTATGGCCGTTGGCGCCGCCATGACTGGCCTACGCCCGATTGTGGAG
GGCATGAACATGGGTTTTCTGCTGCTTGCTTTCAACCAGATCTCCAACAACATGGGAATGCTTCGTTACACCAGTGGCGG
AAATTTCACAATTCCCACCGTGGTGCGTGGGCCTGGTGGTGTGGGGCGCCAACTCGGTGCTGAACATAGTCAGCGACTTG
AGGCCTATTTTCACGCTGTGCCTGGGATCAAGATCGTTGCTTGCAGCACGCCAACCAATGCCAAGGGCTTGATGAAAGCC
GCGATCCGAGACAACAATCCAGTTCTCTTTTTCGAGCATGTGCTGCTCTACAACCTGATTGAGGAGCTCCCAGACGGTGA
TTATGTCTGTGCCCTAGATCAAGCAGATCTGGTTCGTGAGGGTAAAGACGTCACGATCCTCACCTATTCGCGTATGCGTC
ATCACTGTCTCAAGGCTGTTGAACAGTTGGAGGCAGACGGCATCGATGTGGAATTGATCGATTTGATTAGTCTCAAGCCC
TTCGATATGGAGACCATTGTTCGCTCCATCCGTAAAACCCATCGGGTGATTGTGGTTGAGGAGTGTATGAAAACTGGTGG
GATTGGTGCTGAGTTGATTGCGCTGATTACTGAGCAGTGTTTTGACGAACTCGATGCTCGCCCAATTCGCCTCTCCAGTC
AGGACATTCCCACTCCATATAACGGCAAATTGGAGAATTTCACGATCATTCAGCCTCATCAGATTGTTGAAGCGGCTCAG
CAGATTGTTCTTAAGGGGCTTTGA

Upstream 100 bases:

>100_bases
CCGTTTTGCTGCTTTCAGCATTTGCCGCAGGAGGCTAATGCTGCTCAGGCTGAGATAGCTTGGCCTCTTCCACCGGCATG
GCCGGGATTGCAACGAAAAC

Downstream 100 bases:

>100_bases
TTGATGGCACGTCAACAAGGCTGGTTTGCGCTCATTCTTGCTCTTGCCATTGCGGCAGGTTCTGTCACTGCAAGCTTTCC
GCTTGAGTTGGGCCTTGATC

Product: pyruvate dehydrogenase E1 subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSGTLLFNALRDAIDEEMARDSHVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAENSFTGMAVGAAMTGLRPIVE
GMNMGFLLLAFNQISNNMGMLRYTSGGNFTIPTVVRGPGGVGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKA
AIRDNNPVLFFEHVLLYNLIEELPDGDYVCALDQADLVREGKDVTILTYSRMRHHCLKAVEQLEADGIDVELIDLISLKP
FDMETIVRSIRKTHRVIVVEECMKTGGIGAELIALITEQCFDELDARPIRLSSQDIPTPYNGKLENFTIIQPHQIVEAAQ
QIVLKGL

Sequences:

>Translated_327_residues
MSGTLLFNALRDAIDEEMARDSHVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAENSFTGMAVGAAMTGLRPIVE
GMNMGFLLLAFNQISNNMGMLRYTSGGNFTIPTVVRGPGGVGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKA
AIRDNNPVLFFEHVLLYNLIEELPDGDYVCALDQADLVREGKDVTILTYSRMRHHCLKAVEQLEADGIDVELIDLISLKP
FDMETIVRSIRKTHRVIVVEECMKTGGIGAELIALITEQCFDELDARPIRLSSQDIPTPYNGKLENFTIIQPHQIVEAAQ
QIVLKGL
>Mature_326_residues
SGTLLFNALRDAIDEEMARDSHVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAENSFTGMAVGAAMTGLRPIVEG
MNMGFLLLAFNQISNNMGMLRYTSGGNFTIPTVVRGPGGVGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKAA
IRDNNPVLFFEHVLLYNLIEELPDGDYVCALDQADLVREGKDVTILTYSRMRHHCLKAVEQLEADGIDVELIDLISLKPF
DMETIVRSIRKTHRVIVVEECMKTGGIGAELIALITEQCFDELDARPIRLSSQDIPTPYNGKLENFTIIQPHQIVEAAQQ
IVLKGL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=319, Percent_Identity=40.7523510971787, Blast_Score=261, Evalue=5e-70,
Organism=Homo sapiens, GI291084858, Length=319, Percent_Identity=38.5579937304075, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI4557353, Length=322, Percent_Identity=35.0931677018634, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI34101272, Length=322, Percent_Identity=35.0931677018634, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17538422, Length=319, Percent_Identity=43.2601880877743, Blast_Score=274, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI17506935, Length=323, Percent_Identity=35.9133126934984, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6319698, Length=321, Percent_Identity=41.1214953271028, Blast_Score=261, Evalue=2e-70,
Organism=Drosophila melanogaster, GI21358145, Length=321, Percent_Identity=40.4984423676012, Blast_Score=262, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24650940, Length=321, Percent_Identity=40.4984423676012, Blast_Score=262, Evalue=2e-70,
Organism=Drosophila melanogaster, GI160714832, Length=321, Percent_Identity=38.006230529595, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI160714828, Length=321, Percent_Identity=38.006230529595, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24650943, Length=85, Percent_Identity=52.9411764705882, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24650945, Length=85, Percent_Identity=52.9411764705882, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 36048; Mature: 35917

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTLLFNALRDAIDEEMARDSHVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAE
CCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCEEEHHHHHHHHCCEEEEECCCCC
NSFTGMAVGAAMTGLRPIVEGMNMGFLLLAFNQISNNMGMLRYTSGGNFTIPTVVRGPGG
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCEEEEECCCCEEECEEEECCCC
VGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKAAIRDNNPVLFFEHVLLYNLI
CCHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHH
EELPDGDYVCALDQADLVREGKDVTILTYSRMRHHCLKAVEQLEADGIDVELIDLISLKP
HHCCCCCEEEEECCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEHECCCCC
FDMETIVRSIRKTHRVIVVEECMKTGGIGAELIALITEQCFDELDARPIRLSSQDIPTPY
CCHHHHHHHHHHHHCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
NGKLENFTIIQPHQIVEAAQQIVLKGL
CCCCCCEEEECHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGTLLFNALRDAIDEEMARDSHVCVMGEDVGQYGGSYKVTKDLYEKYGELRVLDTPIAE
CCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHCCCCEEEHHHHHHHHCCEEEEECCCCC
NSFTGMAVGAAMTGLRPIVEGMNMGFLLLAFNQISNNMGMLRYTSGGNFTIPTVVRGPGG
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCEEEEECCCCEEECEEEECCCC
VGRQLGAEHSQRLEAYFHAVPGIKIVACSTPTNAKGLMKAAIRDNNPVLFFEHVLLYNLI
CCHHHCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHH
EELPDGDYVCALDQADLVREGKDVTILTYSRMRHHCLKAVEQLEADGIDVELIDLISLKP
HHCCCCCEEEEECCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEHECCCCC
FDMETIVRSIRKTHRVIVVEECMKTGGIGAELIALITEQCFDELDARPIRLSSQDIPTPY
CCHHHHHHHHHHHHCEEEHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
NGKLENFTIIQPHQIVEAAQQIVLKGL
CCCCCCEEEECHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]