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The map label for this gene is dapF [H]

Identifier: 124023206

GI number: 124023206

Start: 1305222

End: 1306193

Strand: Reverse

Name: dapF [H]

Synonym: P9303_15041

Alternate gene names: 124023206

Gene position: 1306193-1305222 (Counterclockwise)

Preceding gene: 124023209

Following gene: 124023205

Centisome position: 48.69

GC content: 46.19

Gene sequence:

>972_bases
ATGTTGCAGTTCAGCAAATATCAGGGTCTCGGCAACGACTTCGTCCTGATTGATGGGCGTGAGGGTCAGTTGTCTGATCA
AGTGATCAATCCTGATCCAGCCTGGGTACGAAAAATCTGTGATCGACACTTCGGGATTGGAGCTGATGGGCTGATTCTTG
CTTTACCACCTAGGGCTGATGGTGATCTGAGAATGCAAATTTTCAATGCTGATGGCAGCCTGGCCGAGATGTGTGGCAAT
GGCATCCGTTGCTTAACACGTTTCTTGGCTGATATCGACGGAGATTTGTGTGCTCAGCGGTGGAACATAGAAACCCTAGC
AGGAATAATTTGTCCAATCTTGCAGGAAGATGGACAAATTTGCGTAGATATGGGATCTCCATTCTTAGACCCAGAATCGA
TACCTACCACCCTTACAATTGGTTCTGCTGGATTGCCCCAGGGCGAGTGCCACTTGGGTGGTACATCATTGAATGTTGCA
GCTGTAGGAATGGGTAATCCGCATGTGATCGTGCCTGTTGAAGATCTTGAAAGTATTCCATTTGAAAAATGGGGGCAAAT
ACTTGAAAAACATCAGACCTTCCCAGCCAAAACAAATGTTCATTTCCTGAAAATACACAGTACGAATCAACTTGAAATTC
GGGTTTGGGAACGTGGGTCGGGACCAACCCTGGCTTGTGGTACAGGAGCCTGTGCATCTCTTGTAGCAGCTTGTTTGCTT
GGTCTTTCTGATGACCATGCGGAGGTTCTTTTACCTGGAGGTGTGTTACAGATCAGTTGGCCAGGGCGTAGGGGTTCTGT
TTTTATGACGGGGCCCGCTGAACCTGTTTTTGACGGGGTTTTGACACCTCTTCTCACGCCAAGTCATACTGAGGTCTTAC
CTCAAGATGATCAGATCAAATTCGTTGAAGACATCGAATATTCTGGAAACATTAAAAGCTCTAGAATTAATAAACACTCC
TATCTTGGATAA

Upstream 100 bases:

>100_bases
GGGATCTCATCGCGCACCCAACCCTGAATCAGACGAATCCCTGGAAGGCTGGGATTGAGAGGGGGGGTCTCCATAGGATT
GGGATCGTTACAAGCTGGTT

Downstream 100 bases:

>100_bases
GATAAAAACCCTGAGGTTGATTCCCTTAAAACGGTGAATGATTGTTCAACTAATCCCTTGTTAGAGAGCATGATCACTCC
TGCTTTTGATTCATTGGGGC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MLQFSKYQGLGNDFVLIDGREGQLSDQVINPDPAWVRKICDRHFGIGADGLILALPPRADGDLRMQIFNADGSLAEMCGN
GIRCLTRFLADIDGDLCAQRWNIETLAGIICPILQEDGQICVDMGSPFLDPESIPTTLTIGSAGLPQGECHLGGTSLNVA
AVGMGNPHVIVPVEDLESIPFEKWGQILEKHQTFPAKTNVHFLKIHSTNQLEIRVWERGSGPTLACGTGACASLVAACLL
GLSDDHAEVLLPGGVLQISWPGRRGSVFMTGPAEPVFDGVLTPLLTPSHTEVLPQDDQIKFVEDIEYSGNIKSSRINKHS
YLG

Sequences:

>Translated_323_residues
MLQFSKYQGLGNDFVLIDGREGQLSDQVINPDPAWVRKICDRHFGIGADGLILALPPRADGDLRMQIFNADGSLAEMCGN
GIRCLTRFLADIDGDLCAQRWNIETLAGIICPILQEDGQICVDMGSPFLDPESIPTTLTIGSAGLPQGECHLGGTSLNVA
AVGMGNPHVIVPVEDLESIPFEKWGQILEKHQTFPAKTNVHFLKIHSTNQLEIRVWERGSGPTLACGTGACASLVAACLL
GLSDDHAEVLLPGGVLQISWPGRRGSVFMTGPAEPVFDGVLTPLLTPSHTEVLPQDDQIKFVEDIEYSGNIKSSRINKHS
YLG
>Mature_323_residues
MLQFSKYQGLGNDFVLIDGREGQLSDQVINPDPAWVRKICDRHFGIGADGLILALPPRADGDLRMQIFNADGSLAEMCGN
GIRCLTRFLADIDGDLCAQRWNIETLAGIICPILQEDGQICVDMGSPFLDPESIPTTLTIGSAGLPQGECHLGGTSLNVA
AVGMGNPHVIVPVEDLESIPFEKWGQILEKHQTFPAKTNVHFLKIHSTNQLEIRVWERGSGPTLACGTGACASLVAACLL
GLSDDHAEVLLPGGVLQISWPGRRGSVFMTGPAEPVFDGVLTPLLTPSHTEVLPQDDQIKFVEDIEYSGNIKSSRINKHS
YLG

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=281, Percent_Identity=35.2313167259786, Blast_Score=191, Evalue=7e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 34818; Mature: 34818

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQFSKYQGLGNDFVLIDGREGQLSDQVINPDPAWVRKICDRHFGIGADGLILALPPRAD
CCCCCCCCCCCCCEEEEECCCCCCCCCEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCC
GDLRMQIFNADGSLAEMCGNGIRCLTRFLADIDGDLCAQRWNIETLAGIICPILQEDGQI
CCEEEEEECCCCHHHHHHCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCCCE
CVDMGSPFLDPESIPTTLTIGSAGLPQGECHLGGTSLNVAAVGMGNPHVIVPVEDLESIP
EEECCCCCCCCCCCCCEEEECCCCCCCCCEEECCCEEEEEEEECCCCEEEEEHHHHHCCC
FEKWGQILEKHQTFPAKTNVHFLKIHSTNQLEIRVWERGSGPTLACGTGACASLVAACLL
HHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHH
GLSDDHAEVLLPGGVLQISWPGRRGSVFMTGPAEPVFDGVLTPLLTPSHTEVLPQDDQIK
CCCCCCCEEEECCCEEEEECCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCEE
FVEDIEYSGNIKSSRINKHSYLG
EEEECCCCCCCCHHCCCCCCCCC
>Mature Secondary Structure
MLQFSKYQGLGNDFVLIDGREGQLSDQVINPDPAWVRKICDRHFGIGADGLILALPPRAD
CCCCCCCCCCCCCEEEEECCCCCCCCCEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCC
GDLRMQIFNADGSLAEMCGNGIRCLTRFLADIDGDLCAQRWNIETLAGIICPILQEDGQI
CCEEEEEECCCCHHHHHHCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCCCE
CVDMGSPFLDPESIPTTLTIGSAGLPQGECHLGGTSLNVAAVGMGNPHVIVPVEDLESIP
EEECCCCCCCCCCCCCEEEECCCCCCCCCEEECCCEEEEEEEECCCCEEEEEHHHHHCCC
FEKWGQILEKHQTFPAKTNVHFLKIHSTNQLEIRVWERGSGPTLACGTGACASLVAACLL
HHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEEEECCCCCEEEECCCHHHHHHHHHHH
GLSDDHAEVLLPGGVLQISWPGRRGSVFMTGPAEPVFDGVLTPLLTPSHTEVLPQDDQIK
CCCCCCCEEEECCCEEEEECCCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCEE
FVEDIEYSGNIKSSRINKHSYLG
EEEECCCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]