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The map label for this gene is cspR [C]

Identifier: 124023171

GI number: 124023171

Start: 1262797

End: 1263297

Strand: Reverse

Name: cspR [C]

Synonym: P9303_14671

Alternate gene names: 124023171

Gene position: 1263297-1262797 (Counterclockwise)

Preceding gene: 124023172

Following gene: 124023170

Centisome position: 47.09

GC content: 52.69

Gene sequence:

>501_bases
ATGAGCGATCCAAGTGAACTCTCACCATTACGCGTAGCTTTATACGAGCCACGGATTCCACCCAATACAGGAAATATCGC
CAGGACCTGTGCGGCCTTCCGCATACCACTAGCTCTGATTGAACCTCTTGGGTTCAGCCTTGATGATCGCTATCTACGTA
GAGCAGGACTTGATTACTGGCCTCACGTTGCTTTGTCAGTTCATTCTGACCTGGATACATTTCGCGAGTGCCTTCCATTA
AATCATCGACTGATCGGTTGCAGCAGATTTGGAGGAATCCCCCTCCGTTCGATGCGTTTCCAAACCGGAGATGTTTTGCT
TTTTGGCCGTGAGGACACCGGGCTTCCCACCAAAATCCGTCAGCAGTGTGATCAGATCACGACAATTCCCATGCCTGGAG
CAGCAAGTGAACAGGGGCAGGGCGGCGTAAGGAGCCTCAACCTTTCTGTGGCTTGCGCCATCGTGAGCTTTGAGGCTGGC
CAGCAACTGGGGCTGTGGTGA

Upstream 100 bases:

>100_bases
TTGGCAAGCTATCTCAGGAACTGGAACCGTTGGCATCAAACAGCTGGCTTGTCATACAGGGTCGAGCATTGAATCTCAAA
CAGCTTGGTATTGCGGTGCC

Downstream 100 bases:

>100_bases
GTAAACCGAACCAAGCTTGCTTCTGCCCCCCCTTGCCTAATCGAGGTGAGATCAGCTAACTTCTCCCGGACTCCAGCCAA
GTTCGGCTTGATAGGGGTTT

Product: SpoU family tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MSDPSELSPLRVALYEPRIPPNTGNIARTCAAFRIPLALIEPLGFSLDDRYLRRAGLDYWPHVALSVHSDLDTFRECLPL
NHRLIGCSRFGGIPLRSMRFQTGDVLLFGREDTGLPTKIRQQCDQITTIPMPGAASEQGQGGVRSLNLSVACAIVSFEAG
QQLGLW

Sequences:

>Translated_166_residues
MSDPSELSPLRVALYEPRIPPNTGNIARTCAAFRIPLALIEPLGFSLDDRYLRRAGLDYWPHVALSVHSDLDTFRECLPL
NHRLIGCSRFGGIPLRSMRFQTGDVLLFGREDTGLPTKIRQQCDQITTIPMPGAASEQGQGGVRSLNLSVACAIVSFEAG
QQLGLW
>Mature_165_residues
SDPSELSPLRVALYEPRIPPNTGNIARTCAAFRIPLALIEPLGFSLDDRYLRRAGLDYWPHVALSVHSDLDTFRECLPLN
HRLIGCSRFGGIPLRSMRFQTGDVLLFGREDTGLPTKIRQQCDQITTIPMPGAASEQGQGGVRSLNLSVACAIVSFEAGQ
QLGLW

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=157, Percent_Identity=42.6751592356688, Blast_Score=110, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18263; Mature: 18132

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPSELSPLRVALYEPRIPPNTGNIARTCAAFRIPLALIEPLGFSLDDRYLRRAGLDYW
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCC
PHVALSVHSDLDTFRECLPLNHRLIGCSRFGGIPLRSMRFQTGDVLLFGREDTGLPTKIR
CCEEEEECCCHHHHHHHCCCCCEEEEECCCCCCCCCCEEECCCCEEEECCCCCCCCHHHH
QQCDQITTIPMPGAASEQGQGGVRSLNLSVACAIVSFEAGQQLGLW
HHHHHEEECCCCCCCCCCCCCCEEEECCEEEEEEEEECCCCCCCCC
>Mature Secondary Structure 
SDPSELSPLRVALYEPRIPPNTGNIARTCAAFRIPLALIEPLGFSLDDRYLRRAGLDYW
CCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCC
PHVALSVHSDLDTFRECLPLNHRLIGCSRFGGIPLRSMRFQTGDVLLFGREDTGLPTKIR
CCEEEEECCCHHHHHHHCCCCCEEEEECCCCCCCCCCEEECCCCEEEECCCCCCCCHHHH
QQCDQITTIPMPGAASEQGQGGVRSLNLSVACAIVSFEAGQQLGLW
HHHHHEEECCCCCCCCCCCCCCEEEECCEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]