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The map label for this gene is gldA [C]

Identifier: 124022697

GI number: 124022697

Start: 900466

End: 901719

Strand: Reverse

Name: gldA [C]

Synonym: P9303_09901

Alternate gene names: 124022697

Gene position: 901719-900466 (Counterclockwise)

Preceding gene: 124022698

Following gene: 124022696

Centisome position: 33.61

GC content: 54.31

Gene sequence:

>1254_bases
ATGACAACAAGCAAATCGTTGTCCGCCATGTCAGCAAAACTACTGCCACACCGGAACTCGCTGGAGCAGGCGTCTGACAC
CTCTCCAACATCAATGAACAAAAACAATTTGCCTGAGGAGGTCTCTCACTGCTCTCACTCCATCGCCCCCGCAAAGGTTG
TTAGGGGAGAAAGGGCTTGGGAAGAAAGCCATCAACTCATTGCCTCGATTTGCCGCTCACCTTTACTTCTTGGACGCAGC
ATCGCCACGGCTGAGTTACGCCAGGGATTACTCAACAACCTCAGGAACCTTGGACTCAACACAGTTTCTGCTGAGCTCAA
GCACGATTGTTGCGAGATGGATCTCAAGCGGATCAATGCTCTTGCCTTGTACAACTCCTGCGACGGCGTGATTGCCGCCG
GTGGGGGCAAGGTGCTAGATGCCGGGAAGCTTTTGGCCCATCGGCTAGGCATCCCATGCATCACAGTTCCACTTAGTGCA
GCCACCTGTGCCGGTTGGACCGCCCTCGCCAATATCTATTCACCAACAGGTGCTTTTCAACGCGATCAAGTTCTGGCTCG
ATGTCCACATCTGATGATCTTTGATCATGGTCTCGTGCGTCAGGCTCCTCCCCGGACTCTCGCAAGCGGCATCGCAGATG
CAATCGCCAAGTGGTACGAAGCGTCTGTCAGCAATGGCTCCAGCAACGATGGCTTGATTCAACAAGCTGTGCAGATGGCA
AGGGTCTTGCGCGATCAGCTGCTGCTTGATGGGCCTGAAGCGCTGAAAGATCAAAACAGCCTGGCCTGGATCAGGGTTGC
CGAAGCCTGCGCACTAACTGCAGGGCTGATCGGAGGGATTGGCGGTTCTCGCTGCCGTACTGCCGCTGCCCACGCGGTAC
ACAACGCACTCACGCAATTAAAAGATTGCCATGAGGTCCTTCATGGAGAAAAGGTGGGTTACGGGATTCTCGTACAGTTG
CGTCTTGAGGAGATTATTGGAGGCCATCAACTGGCCGGACAAGCAAGGCGACAGCTGATTCCATTCCTCAAAGAACTTGA
TCTGCCTGTAAATCTTGAGGATCTGGGCCTATCGAATTTGAGTCTTCACGAGCTGCATGAGGTCTGCCAATTTGCCTGTC
AACAAGGCTCTGACCTTCATCAACTGCCGTTTCCCGTCAATTCCAGCGCCCTACTCGAAGCACTCCTCGGCGCTAGCGAC
AATGGCCCGGTCCCCGTCGAATCCACAGTCACCAAGCGAATGGCAGCCTCTTGA

Upstream 100 bases:

>100_bases
CCGCTGCGAAGGGCTGTCATGCGGCTCATGGAAGACAGTCTTGCCGAGGAAGTGCTTACTGGCCGCATCAAGGACGGTGA
TGCTGCGGAAATGGACGTAG

Downstream 100 bases:

>100_bases
ACCTCAAAACTGGAATGGCAGCCCAAACAATCGCCTCGTCAGAGACCCTTTTACGTCAGCTCACACCACAACTTCTGGAT
CCATTAGCCCGTGAACTGGC

Product: glycerol dehydrogenase

Products: glycerone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MTTSKSLSAMSAKLLPHRNSLEQASDTSPTSMNKNNLPEEVSHCSHSIAPAKVVRGERAWEESHQLIASICRSPLLLGRS
IATAELRQGLLNNLRNLGLNTVSAELKHDCCEMDLKRINALALYNSCDGVIAAGGGKVLDAGKLLAHRLGIPCITVPLSA
ATCAGWTALANIYSPTGAFQRDQVLARCPHLMIFDHGLVRQAPPRTLASGIADAIAKWYEASVSNGSSNDGLIQQAVQMA
RVLRDQLLLDGPEALKDQNSLAWIRVAEACALTAGLIGGIGGSRCRTAAAHAVHNALTQLKDCHEVLHGEKVGYGILVQL
RLEEIIGGHQLAGQARRQLIPFLKELDLPVNLEDLGLSNLSLHELHEVCQFACQQGSDLHQLPFPVNSSALLEALLGASD
NGPVPVESTVTKRMAAS

Sequences:

>Translated_417_residues
MTTSKSLSAMSAKLLPHRNSLEQASDTSPTSMNKNNLPEEVSHCSHSIAPAKVVRGERAWEESHQLIASICRSPLLLGRS
IATAELRQGLLNNLRNLGLNTVSAELKHDCCEMDLKRINALALYNSCDGVIAAGGGKVLDAGKLLAHRLGIPCITVPLSA
ATCAGWTALANIYSPTGAFQRDQVLARCPHLMIFDHGLVRQAPPRTLASGIADAIAKWYEASVSNGSSNDGLIQQAVQMA
RVLRDQLLLDGPEALKDQNSLAWIRVAEACALTAGLIGGIGGSRCRTAAAHAVHNALTQLKDCHEVLHGEKVGYGILVQL
RLEEIIGGHQLAGQARRQLIPFLKELDLPVNLEDLGLSNLSLHELHEVCQFACQQGSDLHQLPFPVNSSALLEALLGASD
NGPVPVESTVTKRMAAS
>Mature_416_residues
TTSKSLSAMSAKLLPHRNSLEQASDTSPTSMNKNNLPEEVSHCSHSIAPAKVVRGERAWEESHQLIASICRSPLLLGRSI
ATAELRQGLLNNLRNLGLNTVSAELKHDCCEMDLKRINALALYNSCDGVIAAGGGKVLDAGKLLAHRLGIPCITVPLSAA
TCAGWTALANIYSPTGAFQRDQVLARCPHLMIFDHGLVRQAPPRTLASGIADAIAKWYEASVSNGSSNDGLIQQAVQMAR
VLRDQLLLDGPEALKDQNSLAWIRVAEACALTAGLIGGIGGSRCRTAAAHAVHNALTQLKDCHEVLHGEKVGYGILVQLR
LEEIIGGHQLAGQARRQLIPFLKELDLPVNLEDLGLSNLSLHELHEVCQFACQQGSDLHQLPFPVNSSALLEALLGASDN
GPVPVESTVTKRMAAS

Specific function: Glycerol utilization. [C]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=364, Percent_Identity=30.4945054945055, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1786815, Length=298, Percent_Identity=33.2214765100671, Blast_Score=128, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.6

Molecular weight: Translated: 44516; Mature: 44384

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSKSLSAMSAKLLPHRNSLEQASDTSPTSMNKNNLPEEVSHCSHSIAPAKVVRGERAW
CCCCHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHCHHHH
EESHQLIASICRSPLLLGRSIATAELRQGLLNNLRNLGLNTVSAELKHDCCEMDLKRINA
HHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LALYNSCDGVIAAGGGKVLDAGKLLAHRLGIPCITVPLSAATCAGWTALANIYSPTGAFQ
HHEECCCCCEEECCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCCCH
RDQVLARCPHLMIFDHGLVRQAPPRTLASGIADAIAKWYEASVSNGSSNDGLIQQAVQMA
HHHHHHHCCCEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RVLRDQLLLDGPEALKDQNSLAWIRVAEACALTAGLIGGIGGSRCRTAAAHAVHNALTQL
HHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KDCHEVLHGEKVGYGILVQLRLEEIIGGHQLAGQARRQLIPFLKELDLPVNLEDLGLSNL
HHHHHHHCCCCCCCHHEEEEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCC
SLHELHEVCQFACQQGSDLHQLPFPVNSSALLEALLGASDNGPVPVESTVTKRMAAS
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TTSKSLSAMSAKLLPHRNSLEQASDTSPTSMNKNNLPEEVSHCSHSIAPAKVVRGERAW
CCCHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHCHHHH
EESHQLIASICRSPLLLGRSIATAELRQGLLNNLRNLGLNTVSAELKHDCCEMDLKRINA
HHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LALYNSCDGVIAAGGGKVLDAGKLLAHRLGIPCITVPLSAATCAGWTALANIYSPTGAFQ
HHEECCCCCEEECCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHCCCCCCH
RDQVLARCPHLMIFDHGLVRQAPPRTLASGIADAIAKWYEASVSNGSSNDGLIQQAVQMA
HHHHHHHCCCEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
RVLRDQLLLDGPEALKDQNSLAWIRVAEACALTAGLIGGIGGSRCRTAAAHAVHNALTQL
HHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KDCHEVLHGEKVGYGILVQLRLEEIIGGHQLAGQARRQLIPFLKELDLPVNLEDLGLSNL
HHHHHHHCCCCCCCHHEEEEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCC
SLHELHEVCQFACQQGSDLHQLPFPVNSSALLEALLGASDNGPVPVESTVTKRMAAS
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NAD+; NADH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.13 {NAD+}} 0.12 {NADH}} 0.06 {dihydroxyacetone}} 0.5 {glycerol}} [C]

Substrates: glycerol; NAD+

Specific reaction: glycerol + NAD+ = glycerone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Chelatingagents; Li+; Na+; Thiolcompounds [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]