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The map label for this gene is cobB [H]

Identifier: 124022647

GI number: 124022647

Start: 850257

End: 851684

Strand: Reverse

Name: cobB [H]

Synonym: P9303_09381

Alternate gene names: 124022647

Gene position: 851684-850257 (Counterclockwise)

Preceding gene: 124022648

Following gene: 124022646

Centisome position: 31.75

GC content: 55.25

Gene sequence:

>1428_bases
ATGGCCTGTGTGATTGCTGCCCCAGCCAGTGGCACTGGGAAAACCTTACTAAGCCTGACCCTCACAGCCTGGGCGCGCAG
CCGTGATCTAAGTCTCCAACCATTCAAGGTGGGGCCCGACTATCTCGATCCGCAACAACTGACGGTTGCTTCCGGCAGAC
CCTGTCGAAACCTTGACCTACTGCTCTGTGGCCCCGACTGGGTGCGAAACAGCTTTCATGGCTTTGGTGCAGCTGCCGAC
CTTGCGCTGGTGGAAGGCGTCATGGGCCTATTCGATGGTGTCGGCACATCGCAGCAAGGCAGTACTGCTGCCGTAGCTCG
TCACCTGCAACTACCTGTCGTGCTTGTCGTGGATGCAAGCGGCCAAGCAGGCTCCCTAGCGGCCCTGGTTAAAGGCTTCC
GAGACCACGATCCACAACTGACTCTGGCCGGGGTGGTGTTGAACCGGGTCAATAGCAATCGCCACAAAGAATTACTGAAA
GAAGTCCTCAACAGCATTGAGGTCAAAGTGCTCGGTTGCTTGCCCCGAGATCCTGAACTCAATCTTCCCAGCAGACATCT
CGGCCTTGCCCCAGCCCATGAGCTTGAGCGATTGGAAGCTCGACTACAAGCCTGGAGCGCCATCGCAGAAACTCATCTCG
ATCTAACGAGCTTCAAAACGTTGCTCCGCGCACCTACCCGGGTAATAGATCCAATTCATGACCTGCTTGGAGAAAAGAGC
ATCCAATCACCACAACCACTACGGCCTGTAGCAGTAGCTCAAGACGATGCCTTTCACTTTCGCTATCCAGAAACCAAAGA
TTGCCTTGAAGCTCTTGGGATGCCAGTGCTGCCCTGGAAACCACTGGAGGATGAACCCCTACCTGCACAAGCGAAAGGTG
TGATCCTTCCAGGCGGCTTCCCCGAACAATATGCGGCGAGTCTTAGTCAATGTGATCGCAGCATGGCAGACCTACGAGCC
TGGTATGGACGGCGCCCGATTTATGCCGAATGTGGCGGGATGCTGCTGCTTGGACAGACCCTCACTGATCTGGAAGGACA
AGCCCATCCGATGACAGGCCTACTGCCTTTTCATGCCAAGAAAGGCTCCCTTCAAGTGGGTTATCGCAGCCTTGATGGCA
CAGAAGACAGTCTTGTTGTACGCAAAGGTGATCGACTCATGGGCCATGAATTCCACCGCTGGGAACTCCACCCCACGGGC
CCTAACGATGTCTTAAGCAACCTTCTTGATCGACCACACAAGATCAACAAACCAAGATCTCTATGGCAAGTTCAAGGGTG
GCGGGTGCAGCAACACAAAGAAGGATGGAGCCATCAGACATTGCATGCCAGCTGGGTGCACCTCCACTGGGCGAGCTGCT
CAATGATCTCCCGTCGATGGCGAGCCGCACTCGAGCCGCAATCAATCCCAGCAACAAGTGATTTCTGA

Upstream 100 bases:

>100_bases
TAACACCACCACCCCCCTGCTGGCCGCTGCAGCCCCCCTTGCTGCCAAGCTGCTTAGCTAGACGCCCTTTGGCGAAGCCC
TTTTTCAGCCGCCACCCGTC

Downstream 100 bases:

>100_bases
ACAGCACAAACCCAGGAAGACATCATGTGACGATGCTCGTCAAAATTCAGGCAGCAGCAACCGCCCTGCATGACATCTCG
TCAACTTTGGATCATCCGCC

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MACVIAAPASGTGKTLLSLTLTAWARSRDLSLQPFKVGPDYLDPQQLTVASGRPCRNLDLLLCGPDWVRNSFHGFGAAAD
LALVEGVMGLFDGVGTSQQGSTAAVARHLQLPVVLVVDASGQAGSLAALVKGFRDHDPQLTLAGVVLNRVNSNRHKELLK
EVLNSIEVKVLGCLPRDPELNLPSRHLGLAPAHELERLEARLQAWSAIAETHLDLTSFKTLLRAPTRVIDPIHDLLGEKS
IQSPQPLRPVAVAQDDAFHFRYPETKDCLEALGMPVLPWKPLEDEPLPAQAKGVILPGGFPEQYAASLSQCDRSMADLRA
WYGRRPIYAECGGMLLLGQTLTDLEGQAHPMTGLLPFHAKKGSLQVGYRSLDGTEDSLVVRKGDRLMGHEFHRWELHPTG
PNDVLSNLLDRPHKINKPRSLWQVQGWRVQQHKEGWSHQTLHASWVHLHWASCSMISRRWRAALEPQSIPATSDF

Sequences:

>Translated_475_residues
MACVIAAPASGTGKTLLSLTLTAWARSRDLSLQPFKVGPDYLDPQQLTVASGRPCRNLDLLLCGPDWVRNSFHGFGAAAD
LALVEGVMGLFDGVGTSQQGSTAAVARHLQLPVVLVVDASGQAGSLAALVKGFRDHDPQLTLAGVVLNRVNSNRHKELLK
EVLNSIEVKVLGCLPRDPELNLPSRHLGLAPAHELERLEARLQAWSAIAETHLDLTSFKTLLRAPTRVIDPIHDLLGEKS
IQSPQPLRPVAVAQDDAFHFRYPETKDCLEALGMPVLPWKPLEDEPLPAQAKGVILPGGFPEQYAASLSQCDRSMADLRA
WYGRRPIYAECGGMLLLGQTLTDLEGQAHPMTGLLPFHAKKGSLQVGYRSLDGTEDSLVVRKGDRLMGHEFHRWELHPTG
PNDVLSNLLDRPHKINKPRSLWQVQGWRVQQHKEGWSHQTLHASWVHLHWASCSMISRRWRAALEPQSIPATSDF
>Mature_474_residues
ACVIAAPASGTGKTLLSLTLTAWARSRDLSLQPFKVGPDYLDPQQLTVASGRPCRNLDLLLCGPDWVRNSFHGFGAAADL
ALVEGVMGLFDGVGTSQQGSTAAVARHLQLPVVLVVDASGQAGSLAALVKGFRDHDPQLTLAGVVLNRVNSNRHKELLKE
VLNSIEVKVLGCLPRDPELNLPSRHLGLAPAHELERLEARLQAWSAIAETHLDLTSFKTLLRAPTRVIDPIHDLLGEKSI
QSPQPLRPVAVAQDDAFHFRYPETKDCLEALGMPVLPWKPLEDEPLPAQAKGVILPGGFPEQYAASLSQCDRSMADLRAW
YGRRPIYAECGGMLLLGQTLTDLEGQAHPMTGLLPFHAKKGSLQVGYRSLDGTEDSLVVRKGDRLMGHEFHRWELHPTGP
NDVLSNLLDRPHKINKPRSLWQVQGWRVQQHKEGWSHQTLHASWVHLHWASCSMISRRWRAALEPQSIPATSDF

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 52276; Mature: 52144

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACVIAAPASGTGKTLLSLTLTAWARSRDLSLQPFKVGPDYLDPQQLTVASGRPCRNLDL
CEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCEEECCCCCCCCCEE
LLCGPDWVRNSFHGFGAAADLALVEGVMGLFDGVGTSQQGSTAAVARHLQLPVVLVVDAS
EEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECC
GQAGSLAALVKGFRDHDPQLTLAGVVLNRVNSNRHKELLKEVLNSIEVKVLGCLPRDPEL
CCCCHHHHHHHHHHCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCCCC
NLPSRHLGLAPAHELERLEARLQAWSAIAETHLDLTSFKTLLRAPTRVIDPIHDLLGEKS
CCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCC
IQSPQPLRPVAVAQDDAFHFRYPETKDCLEALGMPVLPWKPLEDEPLPAQAKGVILPGGF
CCCCCCCCCEEEECCCCEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCC
PEQYAASLSQCDRSMADLRAWYGRRPIYAECGGMLLLGQTLTDLEGQAHPMTGLLPFHAK
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCHHHHCCCCCCCCCCCEECCCC
KGSLQVGYRSLDGTEDSLVVRKGDRLMGHEFHRWELHPTGPNDVLSNLLDRPHKINKPRS
CCCEEECEECCCCCCCCEEEECCCHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCHH
LWQVQGWRVQQHKEGWSHQTLHASWVHLHWASCSMISRRWRAALEPQSIPATSDF
HHHCCCCEEHHHHCCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ACVIAAPASGTGKTLLSLTLTAWARSRDLSLQPFKVGPDYLDPQQLTVASGRPCRNLDL
EEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCEEECCCCCCCCCEE
LLCGPDWVRNSFHGFGAAADLALVEGVMGLFDGVGTSQQGSTAAVARHLQLPVVLVVDAS
EEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECC
GQAGSLAALVKGFRDHDPQLTLAGVVLNRVNSNRHKELLKEVLNSIEVKVLGCLPRDPEL
CCCCHHHHHHHHHHCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEECCCCCCCC
NLPSRHLGLAPAHELERLEARLQAWSAIAETHLDLTSFKTLLRAPTRVIDPIHDLLGEKS
CCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCC
IQSPQPLRPVAVAQDDAFHFRYPETKDCLEALGMPVLPWKPLEDEPLPAQAKGVILPGGF
CCCCCCCCCEEEECCCCEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCC
PEQYAASLSQCDRSMADLRAWYGRRPIYAECGGMLLLGQTLTDLEGQAHPMTGLLPFHAK
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCHHHHCCCCCCCCCCCEECCCC
KGSLQVGYRSLDGTEDSLVVRKGDRLMGHEFHRWELHPTGPNDVLSNLLDRPHKINKPRS
CCCEEECEECCCCCCCCEEEECCCHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCHH
LWQVQGWRVQQHKEGWSHQTLHASWVHLHWASCSMISRRWRAALEPQSIPATSDF
HHHCCCCEEHHHHCCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]