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The map label for this gene is ispA [C]

Identifier: 124022642

GI number: 124022642

Start: 845954

End: 846874

Strand: Direct

Name: ispA [C]

Synonym: P9303_09331

Alternate gene names: 124022642

Gene position: 845954-846874 (Clockwise)

Preceding gene: 124022641

Following gene: 124022643

Centisome position: 31.53

GC content: 54.51

Gene sequence:

>921_bases
ATGAACGAGACAAGGTTCATGGCGGCGGCGGTCAGCACCTTCTTCGATTTCCCCGCCTATTTGGCTGCGGCCAAAGCCAG
CGTTGAAGAGGCTTTGGATGATTCGCTTGGCCCTGAGCGTCCTGAGCAACTCAGGGAGGCCATGCGCTACTCCCTTTTGG
CGGGTGGTAAGCGTTTACGCCCCATCCTTTGCATAGCAGCTTGCGAACTTGCTGGAGGTGAACCGACACAGGCTCTGCCG
ACAGCAGTGGCTTTGGAAATGATTCACACGATGTCGCTGATTCATGACGATCTCCCCGCCATGGATAACGACGATTTACG
CCGCGGTCGTCCCACCAATCACAAGGTTTATGGAGACGCGGTTGCCATTCTCGCGGGTGATGCGCTGCTTACACGTTCCT
TTGAGATGGTTGCCTTGCGCAGCAAGGGTGTTGCGCCTGAGCGGCTGTTGAAGGTTGTTGGCGAGCTTTCTCTGGTTGCT
GGTGCTCCTGGTTTAGTGGGGGGGCAGGTTGTAGATCTGGAGTGTGAGGGCAAAGAGGTGGATCTGGAGACTTTGGAGTT
CATTCATCTCCATAAGACCGGGGCCTTGCTGAGTGCTTGTGTGATCTGCGGAGCGTTGATTGGTGGAGCAGAAGACGATT
TGATTGATGCGCTACGCATCTATGCAAGAGGGATTGGTTTGGCGTTTCAGATCATTGATGACATCCTTGATGTAACGGCG
AGCAGCGATGTTCTTGGCAAAACTGCTGGCAAGGATCTGCTTGCCGATAAGACCACGTATCCCAAATTGTTGGGTCTAGA
GGAATCCCGCCGGCGTGCAGAACTCCTTGTGAGCGAAGCCAAGGCAGCATTAGACCCTTGGCAATCTTCTGCTGCACCTT
TGCTGGCACTAGCCGATTACATCACCAGCCGCGATCGATGA

Upstream 100 bases:

>100_bases
GCTCTTGGTGAATACCGTGGCTAGATGGCAGCAGCATTGCGGTTTGCCCTTCGGCCTGAGAGATTTGCTGGTTTGAGACT
GGTTGGCCTGAGAGAATCCC

Downstream 100 bases:

>100_bases
TTGTCAGCGACTCTTCCCCATCAGCGTGGCTTGGACTTTTAGATAATGCTGTATTGGCTTGGGGGCTTGCGGCTTGTGGC
TTGGCTCAGCTTTCCAAGCT

Product: polyprenyl synthetase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MNETRFMAAAVSTFFDFPAYLAAAKASVEEALDDSLGPERPEQLREAMRYSLLAGGKRLRPILCIAACELAGGEPTQALP
TAVALEMIHTMSLIHDDLPAMDNDDLRRGRPTNHKVYGDAVAILAGDALLTRSFEMVALRSKGVAPERLLKVVGELSLVA
GAPGLVGGQVVDLECEGKEVDLETLEFIHLHKTGALLSACVICGALIGGAEDDLIDALRIYARGIGLAFQIIDDILDVTA
SSDVLGKTAGKDLLADKTTYPKLLGLEESRRRAELLVSEAKAALDPWQSSAAPLLALADYITSRDR

Sequences:

>Translated_306_residues
MNETRFMAAAVSTFFDFPAYLAAAKASVEEALDDSLGPERPEQLREAMRYSLLAGGKRLRPILCIAACELAGGEPTQALP
TAVALEMIHTMSLIHDDLPAMDNDDLRRGRPTNHKVYGDAVAILAGDALLTRSFEMVALRSKGVAPERLLKVVGELSLVA
GAPGLVGGQVVDLECEGKEVDLETLEFIHLHKTGALLSACVICGALIGGAEDDLIDALRIYARGIGLAFQIIDDILDVTA
SSDVLGKTAGKDLLADKTTYPKLLGLEESRRRAELLVSEAKAALDPWQSSAAPLLALADYITSRDR
>Mature_306_residues
MNETRFMAAAVSTFFDFPAYLAAAKASVEEALDDSLGPERPEQLREAMRYSLLAGGKRLRPILCIAACELAGGEPTQALP
TAVALEMIHTMSLIHDDLPAMDNDDLRRGRPTNHKVYGDAVAILAGDALLTRSFEMVALRSKGVAPERLLKVVGELSLVA
GAPGLVGGQVVDLECEGKEVDLETLEFIHLHKTGALLSACVICGALIGGAEDDLIDALRIYARGIGLAFQIIDDILDVTA
SSDVLGKTAGKDLLADKTTYPKLLGLEESRRRAELLVSEAKAALDPWQSSAAPLLALADYITSRDR

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=238, Percent_Identity=30.672268907563, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1786623, Length=300, Percent_Identity=44.6666666666667, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1789578, Length=330, Percent_Identity=28.7878787878788, Blast_Score=118, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17505681, Length=251, Percent_Identity=26.2948207171315, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24651612, Length=246, Percent_Identity=30.0813008130081, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 32680; Mature: 32680

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNETRFMAAAVSTFFDFPAYLAAAKASVEEALDDSLGPERPEQLREAMRYSLLAGGKRLR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHH
PILCIAACELAGGEPTQALPTAVALEMIHTMSLIHDDLPAMDNDDLRRGRPTNHKVYGDA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEHHCC
VAILAGDALLTRSFEMVALRSKGVAPERLLKVVGELSLVAGAPGLVGGQVVDLECEGKEV
HHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCC
DLETLEFIHLHKTGALLSACVICGALIGGAEDDLIDALRIYARGIGLAFQIIDDILDVTA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SSDVLGKTAGKDLLADKTTYPKLLGLEESRRRAELLVSEAKAALDPWQSSAAPLLALADY
CCHHHHHHCCCHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ITSRDR
HHCCCC
>Mature Secondary Structure
MNETRFMAAAVSTFFDFPAYLAAAKASVEEALDDSLGPERPEQLREAMRYSLLAGGKRLR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHH
PILCIAACELAGGEPTQALPTAVALEMIHTMSLIHDDLPAMDNDDLRRGRPTNHKVYGDA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEHHCC
VAILAGDALLTRSFEMVALRSKGVAPERLLKVVGELSLVAGAPGLVGGQVVDLECEGKEV
HHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCC
DLETLEFIHLHKTGALLSACVICGALIGGAEDDLIDALRIYARGIGLAFQIIDDILDVTA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SSDVLGKTAGKDLLADKTTYPKLLGLEESRRRAELLVSEAKAALDPWQSSAAPLLALADY
CCHHHHHHCCCHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ITSRDR
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486607; 7918490 [H]